{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T07:04:05Z","timestamp":1788246245144,"version":"build-2803163510"},"reference-count":0,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2002,1,1]]},"abstract":"<jats:p>\n                    Background This study aimed to characterize the molecular basis of a \u03b2-thalassaemia heterozygote who had a normal haemoglobin A\n                    <jats:sub>2<\/jats:sub>\n                    level. Methods Using haematological and molecular analyses, the -\u03b1 and \u03b2-globin genotypes in a Thai woman with normal haemoglobin A\n                    <jats:sub>2<\/jats:sub>\n                    level, who was identified in a thalassaemia and haemoglobinopathy screening programme, were examined. Results The patient was positive for a 0\u00b736% one-tube osmotic fragility test but negative for the dichlorophenolindophenol dye test for haemoglobin E. Haemoglobin A and A\n                    <jats:sub>2<\/jats:sub>\n                    were observed on cellulose acetate electrophoresis with the haemoglobin A\n                    <jats:sub>2<\/jats:sub>\n                    of 2\u00b70%, compatible with an \u03b1-thalassaemia 1 carrier. Polymerase chain reaction analysis failed to detect \u03b1-thalassaemia 1 (South East Asian deletion). \u03b2-globin gene analysis detected a severe \u03b2\n                    <jats:sup>o<\/jats:sup>\n                    -thalassaemia allele with the 4 bp (-CTTT) deletion at codons 41\/42. Further analysis of the-globin gene identified the homozygosity of the 3\u00b77 kb deletion \u03b1-thalassaemia 2. Conclusions The patient was found to be an unusual case: a \u03b2\n                    <jats:sup>o<\/jats:sup>\n                    -thalassaemia carrier with a low haemoglobin A\n                    <jats:sub>2<\/jats:sub>\n                    concentration, in combination with a homozygosity for the 3\u00b77 kb \u03b1-thalassaemia 2 deletion. The approach used to characterize the patient and the result obtained in this report will prove useful for population screening of thalassaemia in regions where both \u03b1- and \u03b2-thalassaemias are common.\n                  <\/jats:p>","DOI":"10.1258\/0004563021901720","type":"journal-article","created":{"date-parts":[[2002,10,16]],"date-time":"2002-10-16T05:28:56Z","timestamp":1034746136000},"page":"44-49","source":"Crossref","is-referenced-by-count":59,"title":["Molecular analysis of a Thai \u03b2-thalassaemia heterozygote with normal                haemoglobin A\n                    <sub>2<\/sub>\n                    level: implication for population screening"],"prefix":"10.1177","volume":"39","author":[{"given":"Supan","family":"Fucharoen","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goonnapa","family":"Fucharoen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microscopy, Faculty of Associated Medical\r                  Sciences, Khon Kaen University, Khon Kaen, Thailand 40002"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanokwan","family":"Sanchaisuriya","sequence":"additional","affiliation":[{"name":"Department of Clinical Microscopy, Faculty of Associated Medical\r                  Sciences, Khon Kaen University, Khon Kaen, Thailand 40002"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutthana","family":"Pengjam","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2002,1,1]]},"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/0004563021901720","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/0004563021901720","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:51:50Z","timestamp":1777377110000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1258\/0004563021901720"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,1,1]]}},"alternative-id":["10.1258\/0004563021901720"],"URL":"https:\/\/doi.org\/10.1258\/0004563021901720","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1,1]]}}}