{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T03:41:01Z","timestamp":1777434061990,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2003,5,1]]},"abstract":"<jats:p>Background: Serum ornithine carbamoyltransferase is a diagnostic marker of hepatic disorders due to its localization in periportal mitochondria.<\/jats:p>\n                  <jats:p>\n                    Methods: We have developed a new method for the determination of serum ornithine carbamoyltransferase. It is based on the reverse reaction of ornithine carbamoyltransferase, using ornithine-ketoacid aminotransferase, \u2206\n                    <jats:sup>1<\/jats:sup>\n                    -pyrroline-5-carboxylate dehydrogenase and glutamate dehydrogenase, which together convert citrulline through ornithine to glutamate. The glutamate is then quantitatively measured using glutamate oxidase and Trinder's reagent.\n                  <\/jats:p>\n                  <jats:p>\n                    Results: The results obtained by this method agreed well with those obtained using the diacetylmonoxime method as a gold standard [correlation coefficient (r) = 0\u00b7973 P&lt;0\u00b7001]. The endogenous amino acids sensitive to this method in serum (glutamate, ornithine and \u2206\n                    <jats:sup>1<\/jats:sup>\n                    -pyrroline-5-carboxylate) were eliminated by the initial futile reaction. The new method appears to be more accurate at low levels of ornithine carbamoyltransferase activity than the diacetylmonoxime method.\n                  <\/jats:p>\n                  <jats:p>Conclusions: Here we report a new method for serum ornithine carbamoyl-transferase assay which might be useful for clinical diagnosis of hepatic disorders, including hepatic cancer.<\/jats:p>","DOI":"10.1258\/000456303321610583","type":"journal-article","created":{"date-parts":[[2003,5,21]],"date-time":"2003-05-21T20:17:02Z","timestamp":1053548222000},"page":"264-268","source":"Crossref","is-referenced-by-count":8,"title":["A novel method for measuring serum ornithine                carbamoyltransferase"],"prefix":"10.1177","volume":"40","author":[{"given":"Hiroaki","family":"Ishikawa","sequence":"first","affiliation":[{"name":"Department of Medical Technology, Fujita Health University College,\r                  Toyoake, Aichi 470-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeo","family":"Matsuzawa","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, School of Health Sciences, Fujita\r                  Health University, Toyoake, Aichi 470-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Ohashi","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, School of Health Sciences, Fujita\r                  Health University, Toyoake, Aichi 470-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoichi","family":"Nagamura","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, School of Health Sciences, Fujita\r                  Health University, Toyoake, Aichi 470-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2003,5,1]]},"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/000456303321610583","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/000456303321610583","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:52:38Z","timestamp":1777377158000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1258\/000456303321610583"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,5,1]]}},"alternative-id":["10.1258\/000456303321610583"],"URL":"https:\/\/doi.org\/10.1258\/000456303321610583","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,5,1]]}}}