{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:41:09Z","timestamp":1791250869569,"version":"4.1.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2006,3,1]]},"abstract":"<jats:p>We conducted an electrophoretic analysis of monoclonal \u03b3-globulin found in the serum of a patient with splenic marginal-zone lymphoma. This monoclonal protein showed electrophoretic mobility to the \u03b3 region and reacted with antii-mmunoglobulin (IgG) antiserum but not with anti- \u03ba or anti- \u03bb light chain antisera. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting of the monoclonal protein-rich \u03b3-globulin fraction extracted from the sliced gel revealed the presence of two types of abnormal IgG molecule, low- and high-molecular-weight IgG, neither of which reacted with anti- \u03ba or anti- \u03bb light chain antisera. Additionally, an abnormal high-molecular-weight \u03b3 heavy chain was identified by reducing SDS-PAGE. These findings suggest that this monoclonal protein is composed only of \u03b3 heavy chains of normal and larger size. The presence of abnormal serum immunoglobulin composed of only \u03b3 heavy chain has been known as a fundamental feature of \u03b3 heavy chain disease (HCD). However, the unique composition of monoclonal \u03b3-globulin makes our case distinct from typical \u03b3HCD, which is characterized by an abnormal truncated low-molecular-weight \u03b3 heavy chain. Thus, the unusual monoclonal protein may have been produced by a somatic mutation of IgH gene associated with splenic marginal-zone lymphoma.<\/jats:p>","DOI":"10.1258\/000456306776021490","type":"journal-article","created":{"date-parts":[[2006,2,25]],"date-time":"2006-02-25T19:50:51Z","timestamp":1140897051000},"page":"161-164","source":"Crossref","is-referenced-by-count":5,"title":["Unusual\n                    <i>\u03b3<\/i>\n                    heavy chain disease protein in a patient with splenic marginal-zone lymphoma"],"prefix":"10.1177","volume":"43","author":[{"given":"Keiko","family":"Morita","sequence":"first","affiliation":[{"name":"Central Clinical Laboratory, Nara Medical University, Shijo-Cho 840, Kashihara, Nara 634-8522, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Kawamoto","sequence":"additional","affiliation":[{"name":"Central Clinical Laboratory, Nara Medical University, Shijo-Cho 840, Kashihara, Nara 634-8522, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honami","family":"Takada","sequence":"additional","affiliation":[{"name":"Central Clinical Laboratory, Nara Medical University, Shijo-Cho 840, Kashihara, Nara 634-8522, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinobu","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of General Medicine and Clinical Investigation, Nara Medical University, Shijo-Cho 840, Kashihara, Nara 634-8522, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyoshi","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Haematology, Kishiwada City Hospital, Gakuhara-Cho 1001, Kishiwada, Osaka 596-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyuki","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Central Clinical Laboratory, Nara Medical University, Shijo-Cho 840, Kashihara, Nara 634-8522, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2006,3,1]]},"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/000456306776021490","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/000456306776021490","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:54:10Z","timestamp":1777377250000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1258\/000456306776021490"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,3,1]]}},"alternative-id":["10.1258\/000456306776021490"],"URL":"https:\/\/doi.org\/10.1258\/000456306776021490","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,3,1]]}}}