{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T05:39:41Z","timestamp":1741153181998,"version":"3.38.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1995,10,1]],"date-time":"1995-10-01T00:00:00Z","timestamp":812505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lab Anim"],"published-print":{"date-parts":[[1995,10,1]]},"abstract":"<jats:p> Mice homozygous for recessive mutation osteopetrosis ( op\/op) on chromosome 3 provide a unique model to study the mechanism of haematopoiesis in conjunction with bone formation. Based on the DNA sequence data recently reported, we established a PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) assay which identifies an amplified fragment having an insertional point mutation present in macrophage colony-stimulating factor (M-CSF) gene of op\/op mice. With this assay, three genotypes, op\/op, + \/op, and +\/+ can be distinguished. Although heterozygous (+\/ op) and normal (+\/+) mice could not be discriminated phenotypically, we could generate op\/op mutant mice starting from a single heterozygous (+\/ op) mouse using only the PCR-SSCP aided screening method. This assay will permit introduction of the op mutant into any strain to generate a new animal model to study the cytokine network and haematopoiesis. <\/jats:p>","DOI":"10.1258\/002367795780740014","type":"journal-article","created":{"date-parts":[[2007,5,20]],"date-time":"2007-05-20T23:45:00Z","timestamp":1179704700000},"page":"442-446","source":"Crossref","is-referenced-by-count":2,"title":["Non-phenotypic detection of osteopetrotic (<i>op\/op<\/i>) mutation by using PCR-SSCP analysis"],"prefix":"10.1177","volume":"29","author":[{"given":"H.","family":"Kaneko","sequence":"first","affiliation":[{"name":"Department of Pathology, National Children's Medical Research Center Setagaya-ku, Tokyo 154, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Iizuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Morohashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Kokai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Fujimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1995,10,1]]},"container-title":["Laboratory Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/002367795780740014","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/002367795780740014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T15:45:51Z","timestamp":1741103151000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1258\/002367795780740014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,10,1]]}},"alternative-id":["10.1258\/002367795780740014"],"URL":"https:\/\/doi.org\/10.1258\/002367795780740014","relation":{},"ISSN":["0023-6772","1758-1117"],"issn-type":[{"type":"print","value":"0023-6772"},{"type":"electronic","value":"1758-1117"}],"subject":[],"published":{"date-parts":[[1995,10,1]]}}}