{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T05:54:49Z","timestamp":1760853289456,"version":"3.38.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1996,10,1]],"date-time":"1996-10-01T00:00:00Z","timestamp":844128000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lab Anim"],"published-print":{"date-parts":[[1996,10,1]]},"abstract":"<jats:p> Wistar Shionogi rats of the ( od\/od) substrain with the osteogenic disorder are unable to synthesize L-ascorbic acid ( L-AA) and appear to be an appropriate animal model for studying the effect of L-AA in carcinogenesis. To determine the minimal L-AA requirements of these animals for prolonged survival in a satisfactory physical condition during experimentation, four concentrations of L-AA (0.33 g\/l, 0.67 g\/l, 1.67 g\/l and 3.33 g\/l) were administered via drinking water to four groups of animals ( n=2). Their water intake per cage was recorded three times weekly and the plasma L-AA levels were determined at the start, after 2, 4, 8 and 12 weeks and at the termination of the experiment. To simulate the procedures to be undertaken in oral mucosal carcinogenesis experiments, the animals were gently restrained and a designated amount of sterile NaCl was applied to the palatal mucosa three times a week for 26 weeks. <\/jats:p><jats:p> The L-AA supplement group with the lowest concentration (0.33 g\/l L-AA) achieved mean plasma levels of 7 \u00b1 1.38 \u03bcM, approximately one-eighth that of the normal level (mean plasma L-AA level in outbred Wistar rats was found to be 58 \u00b1 3 \u03bcM) whilst those in the higher supplement group (3.33 g\/l L-AA) achieved a mean of 18 \u00b1 1.25 \u03bcM. All of the animals employed in the present study survived for 26 weeks and showed no clinical signs of L-AA deficiency during this period. <\/jats:p>","DOI":"10.1258\/002367796780739826","type":"journal-article","created":{"date-parts":[[2007,5,20]],"date-time":"2007-05-20T23:45:00Z","timestamp":1179704700000},"page":"337-346","source":"Crossref","is-referenced-by-count":12,"title":["Determination of the <i>L<\/i>-ascorbic acid requirements in Wistar osteogenic disorder Shionogi rats for prolonged carcinogenesis experiments"],"prefix":"10.1177","volume":"30","author":[{"given":"S. W. Y.","family":"Chan","sequence":"first","affiliation":[{"name":"Oral Medicine & Surgery Research Unit, School of Dental Science, University of Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. C.","family":"Reade","sequence":"additional","affiliation":[{"name":"Oral Medicine & Surgery Research Unit, School of Dental Science, University of Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1996,10,1]]},"container-title":["Laboratory Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/002367796780739826","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/002367796780739826","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T07:54:20Z","timestamp":1740988460000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1258\/002367796780739826"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1996,10,1]]}},"alternative-id":["10.1258\/002367796780739826"],"URL":"https:\/\/doi.org\/10.1258\/002367796780739826","relation":{},"ISSN":["0023-6772","1758-1117"],"issn-type":[{"type":"print","value":"0023-6772"},{"type":"electronic","value":"1758-1117"}],"subject":[],"published":{"date-parts":[[1996,10,1]]}}}