{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T16:18:55Z","timestamp":1772813935619,"version":"3.50.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2001,12,1]],"date-time":"2001-12-01T00:00:00Z","timestamp":1007164800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J STD AIDS"],"published-print":{"date-parts":[[2001,12,1]]},"abstract":"<jats:p> Detection of Haemophilus ducreyi in genital ulcer specimens by culture lacks sensitivity. To enhance detection, a heminested polymerase chain reaction (PCR) assay was developed targeting the nucleotide sequence of a gene, designated p27, which encodes for a 27 kDa H. ducreyi-specific protein. The p27 PCR assay detected all (37\/37) H. ducreyi strains tested and gave no amplified product from DNA extracts of any of 31 other microorganisms, from 30 non-genital ulcer specimens, or from 29 urethral and vaginal swab specimens collected from non-chancroid STD patients. In genital ulcer disease specimens, compared to combined positive results obtained by culture and a previously described PCR assay, the p27 PCR assay showed a sensitivity of 91% (48\/53). The p27 PCR assay provides a specific and a sensitive detection of H. ducreyi in clinical specimens. <\/jats:p>","DOI":"10.1258\/0956462011924425","type":"journal-article","created":{"date-parts":[[2002,10,16]],"date-time":"2002-10-16T09:48:30Z","timestamp":1034761710000},"page":"797-803","source":"Crossref","is-referenced-by-count":9,"title":["Development of a heminested polymerase chain reaction assay for the                detection of <i>Haemophilus ducreyi<\/i> in clinical                specimens"],"prefix":"10.1177","volume":"12","author":[{"given":"Tsegaye","family":"Tekle-Michael","sequence":"first","affiliation":[{"name":"Department of Microbiology, Institute of Tropical Medicine, Antwerp,\r                  Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eddy","family":"Van Dyck","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Tropical Medicine, Antwerp,\r                  Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sa\u00efd","family":"Abdellati","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Tropical Medicine, Antwerp,\r                  Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Laga","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Tropical Medicine, Antwerp,\r                  Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2001,12,1]]},"container-title":["International Journal of STD &amp; AIDS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/0956462011924425","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/0956462011924425","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T03:59:38Z","timestamp":1741060778000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1258\/0956462011924425"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,12,1]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2001,12,1]]}},"alternative-id":["10.1258\/0956462011924425"],"URL":"https:\/\/doi.org\/10.1258\/0956462011924425","relation":{},"ISSN":["0956-4624","1758-1052"],"issn-type":[{"value":"0956-4624","type":"print"},{"value":"1758-1052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,12,1]]}}}