{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T07:17:46Z","timestamp":1793085466093,"version":"4.5.0"},"reference-count":12,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2009,9,3]],"date-time":"2009-09-03T00:00:00Z","timestamp":1251936000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2009,11]]},"abstract":"\n Background<\/jats:title>\n Circulating cell-free DNA (CFD) levels may be elevated in trauma, stroke, sepsis, pre-eclampsia and cancer. Owing to the complex and expensive methodology, detection of CFD has hitherto been confined to research laboratories. This study presents a simple, inexpensive and accurate test for CFD.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Using the commercial fluorescent SYBR\n \u00ae<\/jats:sup>\n Gold stain, biological fluids were directly assayed for CFD without prior DNA extraction and amplification. Stain was added to the sample in 96-well plates (final stain dilution: 1:10,000) and fluorescence was read by a fluorometer (excitation wavelength 488 nm, emission wavelength 535 nm).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The assay was validated with serum, whole blood, urine and supernatant of cell cultures. Specificity and linearity were demonstrated over a wide range of concentrations; the results correlated with the conventional quantitative polymerase chain reaction assay of \u03b2-globin ( R\n 2<\/jats:sup>\n = 0.9987, P < 0.001). The assay was not affected by exposure of whole blood or serum to room temperature for four or 24 h, respectively. Intra and day-to-day coefficients of variation (16\u20134.8% and 31\u20138%, respectively; depending on DNA level) compared well with published data describing more work-intensive tests. The limit of quantitation (170 ng\/mL) was below the mean DNA level in a cohort of normal individuals (471 [203] ng\/mL). Finally, free DNA in supernatant of cell cultures after cell lysis accurately reflected cell number ( R\n 2<\/jats:sup>\n = 0.974, P < 0.0001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n The direct SYBR\n \u00ae<\/jats:sup>\n Gold assay proved to be an accurate and simple technique for measuring CFD in biological fluids.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1258\/acb.2009.009002","type":"journal-article","created":{"date-parts":[[2009,9,3]],"date-time":"2009-09-03T21:49:58Z","timestamp":1252014598000},"page":"488-494","source":"Crossref","is-referenced-by-count":114,"title":["A rapid direct fluorescent assay for cell-free DNA quantification in biological fluids"],"prefix":"10.1177","volume":"46","author":[{"given":"Hagit","family":"Goldshtein","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, Ben-Gurion University of the Negev"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J","family":"Hausmann","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Soroka Medical University Center, Beer-Sheva, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amos","family":"Douvdevani","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Ben-Gurion University of the Negev"},{"name":"Department of Nephrology, Soroka Medical University Center, Beer-Sheva, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2009,9,3]]},"reference":[{"key":"bibr-ACB-09-002C1","doi-asserted-by":"publisher","DOI":"10.1186\/cc4894"},{"key":"bibr-ACB-09-002C2","doi-asserted-by":"publisher","DOI":"10.1016\/S0305-7372(02)00077-4"},{"key":"bibr-ACB-09-002C3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2003.12.019"},{"key":"bibr-ACB-09-002C4","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/46.3.319"},{"key":"bibr-ACB-09-002C5","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2005.05.021"},{"key":"bibr-ACB-09-002C6","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.191"},{"key":"bibr-ACB-09-002C7","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1998.3067"},{"key":"bibr-ACB-09-002C8","doi-asserted-by":"publisher","DOI":"10.1373\/49.6.1028"},{"key":"bibr-ACB-09-002C9","doi-asserted-by":"publisher","DOI":"10.1172\/JCI102492"},{"key":"bibr-ACB-09-002C10","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105479"},{"key":"bibr-ACB-09-002C11","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1368.026"},{"key":"bibr-ACB-09-002C12","first-page":"4675","volume":"61","author":"Sozzi G","year":"2001","journal-title":"Cancer Res"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/acb.2009.009002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1258\/acb.2009.009002","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/acb.2009.009002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:55:39Z","timestamp":1777377339000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1258\/acb.2009.009002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,3]]},"references-count":12,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,11]]}},"alternative-id":["10.1258\/acb.2009.009002"],"URL":"https:\/\/doi.org\/10.1258\/acb.2009.009002","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,3]]}}}