{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:56:00Z","timestamp":1792018560269,"version":"4.4.0"},"reference-count":18,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The clinical requirements of the users of assay results must be at the centre of assay development. We aimed to develop a single liquid chromatography tandem mass spectrometry (LC-MS\/MS) assay for drugs of abuse in urine that would meet the needs of our service users and replace the multiple screening and confirmatory techniques previously in use.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>After discussion with our users, it was decided that 13 drugs and metabolites should be measured in our panel: morphine, codeine, norcodeine, dihydrocodeine, 6-monoacetylmorphine, acetyl codeine, methadone and its metabolite, buprenorphine and its metabolite, amphetamine, benzoylecgonine and cotinine. Urine samples were prepared by the addition of internal standard, enzymatic hydrolysis and solid-phase extraction. Chromatography conditions were optimized so that the analytes were separated within a run time of 6 min. Optimal parent to daughter m\/z ion transitions were chosen for all drugs and daughter ion ratios were used.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The LC-MS\/MS assay was successfully validated with acceptable precision and lower limits of quantification for all drugs. No matrix effects were seen. The results produced by the LC-MS\/MS assay compared well with the previous combination of techniques in use.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We have developed and validated a fit-for-purpose LC-MS\/MS assay for 13 drugs of abuse in urine that obviates the need for multiple screening and confirmatory analytical techniques.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1258\/acb.2010.010049","type":"journal-article","created":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T22:12:43Z","timestamp":1278022363000},"page":"415-422","source":"Crossref","is-referenced-by-count":13,"title":["Development of a clinically relevant liquid chromatography tandem mass spectrometry assay for 13 drugs of abuse in urine, designed to meet the needs of the service users"],"prefix":"10.1177","volume":"47","author":[{"given":"K","family":"Duxbury","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, Salford Royal NHS Foundation Trust, Stott Lane, Salford M6 8HD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Romagnoli","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Salford Royal NHS Foundation Trust, Stott Lane, Salford M6 8HD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Salford Royal NHS Foundation Trust, Stott Lane, Salford M6 8HD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Watts","sequence":"additional","affiliation":[{"name":"Waters Corporation, MS Technologies Centre, Atlas Park, Simonsway, Manchester, M22 5PP, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Waite","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Salford Royal NHS Foundation Trust, Stott Lane, Salford M6 8HD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2010,7,1]]},"reference":[{"key":"bibr-ACB-10-049C1","unstructured":"www.dh.gov.uk\/en\/Publichealth\/Healthimprovement\/Drugmisuse\/DH_085886 (last checked December 2009)"},{"key":"bibr-ACB-10-049C2","doi-asserted-by":"publisher","DOI":"10.1136\/oem.2002.006163"},{"key":"bibr-ACB-10-049C3","doi-asserted-by":"publisher","DOI":"10.1177\/000456329503200203"},{"key":"bibr-ACB-10-049C4","doi-asserted-by":"publisher","DOI":"10.1177\/000456329703400502"},{"key":"bibr-ACB-10-049C5","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/29.5.359"},{"key":"bibr-ACB-10-049C6","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2009.07.019"},{"key":"bibr-ACB-10-049C7","doi-asserted-by":"publisher","DOI":"10.1080\/15563650802516160"},{"key":"bibr-ACB-10-049C8","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep.2008.09.002"},{"key":"bibr-ACB-10-049C9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1360-0443.2005.01039.x"},{"key":"bibr-ACB-10-049C10","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/47.2.301"},{"key":"bibr-ACB-10-049C11","unstructured":"www.heathcontrol.com (last checked December 2009)"},{"key":"bibr-ACB-10-049C12","doi-asserted-by":"publisher","DOI":"10.1258\/000456307781645996"},{"key":"bibr-ACB-10-049C13","first-page":"8","volume":"221","author":"European Union Decision 2002\/657\/EC 17.08.2002","year":"2002","journal-title":"Off J Eur Commun"},{"key":"bibr-ACB-10-049C14","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/26.8.561"},{"key":"bibr-ACB-10-049C15","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/31.1.1"},{"key":"bibr-ACB-10-049C16","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2006.04.019"},{"key":"bibr-ACB-10-049C17","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/31.7.359"},{"key":"bibr-ACB-10-049C18","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.09.017"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/acb.2010.010049","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1258\/acb.2010.010049","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/acb.2010.010049","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:56:03Z","timestamp":1777377363000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1258\/acb.2010.010049"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,1]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1258\/acb.2010.010049"],"URL":"https:\/\/doi.org\/10.1258\/acb.2010.010049","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,1]]}}}