{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:24:11Z","timestamp":1785907451052,"version":"3.56.0"},"reference-count":13,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Dexamethasone is a synthetic glucocorticoid and is analogous to cortisol. It is used in the low-dose overnight dexamethasone suppression test (LDODST) to diagnose hypercortisolism in patients suspected to be suffering from Cushing's syndrome (CS). Measuring plasma dexamethasone in conjunction with measuring the amount of cortisol following the LDODST may allow clinicians to improve the diagnosis of CS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Plasma samples were cleaned up by solid-phase extraction before analysis. Liquid chromatographic separation was carried out under reversed-phase conditions prior to detection by tandem mass spectrometry. The analytes were determined in the presence of deuterated internal standards cortisol-d4 and dexamethasone-d4.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Limit of quantitation (LOQ) was 1.89 nmol\/L for dexamethasone and &lt;0.02 \u03bcmol\/L for cortisol. Recoveries of both analytes ranged from 80.2% to 114.4%. Intra- and interassay coefficients of variation were &lt;15%. The concentration of dexamethasone and cortisol was determined in 62 patients after performing LDODST. Dexamethasone concentrations ranged from 3.0 to 21.5 nmol\/L (median 7.4 nmol\/L) for 57 of these samples. For five patients the concentration was &lt;LOQ. Cortisol concentrations were &lt;0.08 \u03bcmol\/L (median 0.037 \u03bcmol\/L, n = 54) except for eight patients (&gt;0.22 \u03bcmol\/L).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A method for the simultaneous measurement of dexamethasone and cortisol in human plasma by liquid chromatography\/tandem mass spectrometry has been developed and validated. The method is suitable for controlling the compliance to the LDODST and for determining the cortisol plasma concentration after the test. The interpretation of LDODSTs was improved by the simultaneous determination of both analytes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1258\/acb.2011.011004","type":"journal-article","created":{"date-parts":[[2012,1,13]],"date-time":"2012-01-13T01:03:12Z","timestamp":1326416592000},"page":"170-176","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":32,"title":["Dexamethasone suppression test: development of a method for simultaneous determination of cortisol and dexamethasone in human plasma by liquid chromatography\/tandem mass spectrometry"],"prefix":"10.1177","volume":"49","author":[{"given":"C","family":"Hempen","sequence":"first","affiliation":[{"name":"Medisch Spectrum Twente Laboratory, PO Box 50000, 7500 KA, Enschede"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Elfering","sequence":"additional","affiliation":[{"name":"Medisch Spectrum Twente Laboratory, PO Box 50000, 7500 KA, Enschede"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A H L","family":"Mulder","sequence":"additional","affiliation":[{"name":"Hospital Group Twente Laboratory, PO Box 546, 7550 AM Hengelo, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F A J T M","family":"van den Bergh","sequence":"additional","affiliation":[{"name":"Medisch Spectrum Twente Laboratory, PO Box 50000, 7500 KA, Enschede"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R G H J","family":"Maatman","sequence":"additional","affiliation":[{"name":"Hospital Group Twente Laboratory, PO Box 546, 7550 AM Hengelo, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,3,1]]},"reference":[{"key":"bibr-ACB-11-004C1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01844196"},{"key":"bibr-ACB-11-004C2","doi-asserted-by":"publisher","DOI":"10.1016\/S0967-5868(99)90031-0"},{"key":"bibr-ACB-11-004C3","volume-title":"Basic and Clinical Endocrinology","author":"Greenspan FS","year":"1991","edition":"3"},{"key":"bibr-ACB-11-004C4","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2008-0125"},{"key":"bibr-ACB-11-004C5","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-27302007000800006"},{"key":"bibr-ACB-11-004C6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.1982.tb00733.x"},{"key":"bibr-ACB-11-004C7","doi-asserted-by":"publisher","DOI":"10.1192\/bjp.148.1.66"},{"key":"bibr-ACB-11-004C8","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3223(96)00094-7"},{"key":"bibr-ACB-11-004C9","doi-asserted-by":"publisher","DOI":"10.1081\/JLC-120004749"},{"key":"bibr-ACB-11-004C10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.12.015"},{"key":"bibr-ACB-11-004C11","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(91)90312-S"},{"key":"bibr-ACB-11-004C12","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-007-9291-7"},{"key":"bibr-ACB-11-004C13","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.033605"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/acb.2011.011004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1258\/acb.2011.011004","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/acb.2011.011004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:56:39Z","timestamp":1777377399000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1258\/acb.2011.011004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1258\/acb.2011.011004"],"URL":"https:\/\/doi.org\/10.1258\/acb.2011.011004","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3]]}}}