{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T04:08:39Z","timestamp":1777435719639,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2012,7,24]],"date-time":"2012-07-24T00:00:00Z","timestamp":1343088000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2012,9]]},"abstract":"\n Background<\/jats:title>\n \n Accurate measurement of haemoglobin A\n 1c<\/jats:sub>\n (HbA\n 1c<\/jats:sub>\n ) is useful for long-term glycaemic control in patients with diabetes. Many Hb variants can interfere with HbA\n 1c<\/jats:sub>\n measurement and cause inaccurate results.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The subject was a 31-year-old Thai man who was discovered because of an unexpected HbA\n 1c<\/jats:sub>\n result; other diabetic parameters were within the normal range. Abnormal Hb was investigated using automated high-pressure liquid chromatography (HPLC) and a capillary electrophoresis system. Mutation analysis was done by cDNA sequencing, polymerase chain reaction\u2013restriction fragment length polymorphism (PCR\u2013RFLP) and multiplex allele-specific PCR assays.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Evaluation of HbA\n 1c<\/jats:sub>\n by cation-exchange HPLC showed a value of 34.9% (reference interval, 4.0\u20136.0%), but a value of only 4.0% (reference value, 4.8\u20135.9%) was found with a turbidimetric immunoassay. Haematological analysis revealed a mild anaemia but other parameters were within the normal range. Hb-HPLC analysis demonstrated an unknown Hb variant (47.0%) separating from HbA (46.7%), but capillary electrophoresis identified no abnormal peaks. Mutation analysis identified the Hb Raleigh ( \u03b21[NA1]Val \u2192 Ala [G TG \u2192 G CG]) mutation in combination with an \u03b1\n +<\/jats:sup>\n -thalassaemia, a hitherto undescribed association. The Hb Raleigh mutation could be detected by PCR\u2013RFLP or a multiplex allele-specific PCR assay.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Hb Raleigh can cause falsely increased HbA\n 1c<\/jats:sub>\n values on cation-exchange HPLC. Definitive diagnosis of this variant using combined Hb and DNA analyses is therefore essential.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1258\/acb.2012.011234","type":"journal-article","created":{"date-parts":[[2012,7,24]],"date-time":"2012-07-24T20:17:41Z","timestamp":1343161061000},"page":"445-449","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["A spurious haemoglobin A\n 1c<\/sub>\n result associated with double heterozygote for haemoglobin Raleigh (\n \u03b2<\/i>\n 1[NA1]Val \u2192 Ala) and\n \u03b1<\/i>\n +<\/sup>\n -thalassaemia"],"prefix":"10.1177","volume":"49","author":[{"given":"Kritsada","family":"Singha","sequence":"first","affiliation":[{"name":"The Graduate School"},{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goonnapa","family":"Fucharoen","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Attawut","family":"Chaibunruang","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paripat","family":"Netnee","sequence":"additional","affiliation":[{"name":"Regional Health Promotion Center, Region 5, Nakhon Ratchasima, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Supan","family":"Fucharoen","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 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