{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T03:09:55Z","timestamp":1792465795145,"version":"4.5.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2012,8,8]],"date-time":"2012-08-08T00:00:00Z","timestamp":1344384000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Teicoplanin is an antibiotic used for the treatment of endocarditis, osteomyelitis, septic arthritis and methicillin-resistant Staphylococcus aureus. Teicoplanin is emerging as a suitable alternative antibiotic to vancomycin, where their trough serum levels are monitored by immunoassay routinely. This is the first report detailing the development of a liquid chromatography-tandem mass spectrometry (LC-MS\/MS) method for measuring teicoplanin in patients' serum.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>An Acquity\u2122 UPLC (ultra-pressure liquid chromatography) tandem mass spectrometer was used to measure teicoplanin concentrations in samples from patients, quality assurance schemes and quality control preparations. Ristocetin was successfully implemented as a suitable internal standard. Ion suppression, linearity, stability, matrix effects, recovery, imprecision, lower limits of quantification and detection, interference and method comparison against immunoassay were all assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Teicoplanin and ristocetin had elution times of 1.39 and 1.24 min, respectively. Ion suppression was shown to be negligible, and linear calibration curves (0\u2013200 \u03bcg\/mL) were consistently reproduced to have r\n                      <jats:sup>2<\/jats:sup>\n                      values &gt;0.99. Postextraction stability was achieved up to 20 h, while matrix effects were minimal coupled with sample recovery of &gt;93%. The lower limit of quantification was 1 \u03bcg\/mL, and 0.2 \u03bcg\/mL was the lower limit of detection. Interference with other antibiotics was dependent on the combination of drugs present in patients\u2019 serum. A method comparison between immunoassay and LC-MS\/MS suggested a negative bias for tandem mass spectrometry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This novel method of teicoplanin determination by LC-MS\/MS is proven to be a robust protocol that is consistent and reproducible. Clinicians searching for alternatives in therapeutic drug monitoring may have an additional option that is potentially more accurate and specific.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1258\/acb.2012.011257","type":"journal-article","created":{"date-parts":[[2012,8,9]],"date-time":"2012-08-09T01:43:58Z","timestamp":1344476638000},"page":"475-481","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":24,"title":["Measurement of teicoplanin by liquid chromatography-tandem mass spectrometry: development of a novel method"],"prefix":"10.1177","volume":"49","author":[{"given":"Francis H Y","family":"Fung","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry and Metabolic Medicine, Royal Liverpool and Broadgreen University Hospital, 4th floor Duncan Building, Liverpool L7 8XP"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan C 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