{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:14:25Z","timestamp":1791288865421,"version":"4.3.0"},"reference-count":24,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Measurements of plasma normetanephrine (NMN) and metanephrine (MN) provide a sensitive test for diagnosis of phaeochromocytomas and paragangliomas (PPGLs), but do not allow detection of dopamine-producing tumours. Here we introduce a novel mass spectrometric based method coupled to ultraperformance liquid chromatography (LC-MS\/MS) for measuring NMN, MN and 3-methoxytyramine (MTY), the O-methylated metabolite of dopamine.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Specific collision-induced fragment ions assessed by multireaction monitoring transitions were used for identification, with quantification according to signal intensities of analytes relative to stable isotope labelled internal standards. Results for solid-phase extracted samples from 196 subjects analysed by LC-MS\/MS were compared with those analysed by liquid chromatography with electrochemical detection (LC-ECD). Concentration ranges in 125 volunteers were compared with those from 63 patients with PPGLs, including 14 with metastatic disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The LC-MS\/MS method showed linearity over four orders of magnitude with analytical sensitivity sufficient to measure to 0.02 nmol\/L. Intra- and inter-assay coefficients of variation ranged from 2.8% to 13.5%. NMN and MN were respectively measured 17% and 10% higher and MTY 26% lower by LC-MS\/MS than by LC-ECD. Medians and ranges for 3-methoxytramine, NMN and MN were respectively 0.08 (0.03-0.13), 0.35 (0.13-0.95) and 0.15 (0.07-0.33) nmol\/L in volunteers. Among patients with PPGLs, plasma methoxytyramine was six-fold higher in patients with than without metastastases (1.09 versus 0.19 nmol\/L) and in three patients was the only metabolite increased.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The LC-MS\/MS method enables accurate, selective and sensitive measurements of catecholamine O-methylated metabolites that should be particularly useful for screening and management of dopamine-producing metastatic PPGLs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1258\/acb.2012.012112","type":"journal-article","created":{"date-parts":[[2013,4,15]],"date-time":"2013-04-15T20:07:26Z","timestamp":1366056446000},"page":"147-155","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":110,"title":["Analysis of plasma 3-methoxytyramine, normetanephrine and metanephrine by ultraperformance liquid chromatographytandem mass spectrometry: utility for diagnosis of dopamine-producing metastatic phaeochromocytoma"],"prefix":"10.1177","volume":"50","author":[{"given":"Mirko","family":"Peitzsch","sequence":"first","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine, University of Dresden, Fetscherstra\u00dfe 74, Dresden 01307, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksander","family":"Prejbisz","sequence":"additional","affiliation":[{"name":"Department of Hypertension, Institute of Cardiology, Warsaw, Poland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Kroi\u00df","sequence":"additional","affiliation":[{"name":"Endocrine and Diabetes Unit, Medical University Hospital, W\u00fcrzburg;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Beuschlein","sequence":"additional","affiliation":[{"name":"Endocrine Research Unit, Medical Clinic Innenstadt, LMU, Munich, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiebke","family":"Arlt","sequence":"additional","affiliation":[{"name":"Centre for Endocrinology, Diabetes and Metabolism, University of Birmingham, Birmingham, UK;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Januszewicz","sequence":"additional","affiliation":[{"name":"Department of Hypertension, Institute of Cardiology, Warsaw, Poland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Siegert","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine, University of Dresden, Fetscherstra\u00dfe 74, Dresden 01307, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graeme","family":"Eisenhofer","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine, University of Dresden, Fetscherstra\u00dfe 74, Dresden 01307, Germany;"},{"name":"Department of Medicine III, University of Dresden, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,3,11]]},"reference":[{"key":"bibr1-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2004.01.015"},{"key":"bibr2-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.11.1427"},{"key":"bibr3-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2010.153320"},{"key":"bibr4-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-1862"},{"key":"bibr5-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2011.07.016"},{"key":"bibr6-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/39.1.97"},{"key":"bibr7-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1296.072"},{"key":"bibr8-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1353.040"},{"key":"bibr9-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-008-0162-2"},{"key":"bibr10-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1224133"},{"key":"bibr11-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.024703"},{"key":"bibr12-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.104083"},{"key":"bibr13-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2010.01.012"},{"key":"bibr14-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.06.013"},{"key":"bibr15-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2011.010235"},{"key":"bibr16-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2007.087114"},{"key":"bibr17-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2009.009153"},{"key":"bibr18-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/48.3.533"},{"key":"bibr19-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1276"},{"key":"bibr20-acb.2012.012112","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijnurstu.2009.10.001"},{"key":"bibr21-acb.2012.012112","author":"Eisenhofer 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