{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T05:44:09Z","timestamp":1777873449311,"version":"3.51.4"},"reference-count":30,"publisher":"Frontiers Media SA","issue":"10","license":[{"start":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T00:00:00Z","timestamp":1317427200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Exp Biol Med (Maywood)"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:p>Patagonfibrase is a P-III class metalloproteinase isolated from the venom of Philodryas patagoniensis, a South-American, rear-fanged \u2018colubrid\u2019 snake responsible for accidents with clinical significance. Since local inflammatory reactions are conspicuous signs of snakebites inflicted by this species and taking into consideration that most snake venom metalloproteinases exhibit inflammatory activity, this study deals with the proinflammatory effects evoked by patagonfibrase. Herein, we demonstrate that patagonfibrase causes a time- and dose-dependent hemorrhagic edema when injected into mouse hind paws. The peak of edema occurred at 30 min after injection, and the minimum edematogenic dose was 0.021 \u03bcg. By histological analysis, the presence of moderate to marked edema and hemorrhage, and a mild inflammatory infiltrate was observed. When injected subcutaneously into the scrotal bag of mice, patagonfibrase induced cell recruitment with a significant alteration in physiological parameters of leukocyte\u2013endothelium interaction. The presence of 1 mmol\/L o-phenanthroline, which chelates metal ions, significantly inhibited the proinflammatory effects induced by patagonfibrase. Taken together, these results imply that patagonfibrase is an important contributor to local inflammation elicited by P. patagoniensis envenomation, which may pave the way for novel therapeutic strategies to treat this snakebite. Moreover, our findings demonstrate for the first time that a venom metalloproteinase from a rear-fanged snake elicits proinflammatory effects mainly mediated by its catalytic activity.<\/jats:p>","DOI":"10.1258\/ebm.2011.011125","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T20:25:35Z","timestamp":1314908735000},"page":"1166-1172","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["Inflammatory effects of patagonfibrase, a metalloproteinase from\n                    <i>Philodryas patagoniensis<\/i>\n                    (Patagonia Green Racer; Dipsadidae) venom"],"prefix":"10.3389","volume":"236","author":[{"given":"Mar\u00eda Elisa","family":"Peichoto","sequence":"first","affiliation":[{"name":"C\u00e1tedra de Farmacolog\u00eda, Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste, Sargento Cabral 2139, 3400 Corrientes, Argentina"},{"name":"Laborat\u00f3rio de Fisiopatologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900 S\u00e3o Paulo-SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca Cestari","family":"Zychar","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Fisiopatologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900 S\u00e3o Paulo-SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1vio Luiz","family":"Tavares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Fisiopatologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900 S\u00e3o Paulo-SP, Brazil"},{"name":"C\u00e1tedra de Fisiolog\u00eda, Facultad de Ingenier\u00eda, Universidad de la Cuenca del Plata, Lavalle 50, 3400 Corrientes, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Roberto","family":"de Camargo Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Fisiopatologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900 S\u00e3o Paulo-SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ofelia","family":"Acosta","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Farmacolog\u00eda, Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste, Sargento Cabral 2139, 3400 Corrientes, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Larami","family":"Santoro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Fisiopatologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900 S\u00e3o Paulo-SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2011,10,1]]},"reference":[{"key":"bibr-EBM-1104-RM-125C1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420008661.sec1"},{"key":"bibr-EBM-1104-RM-125C2","first-page":"115","volume-title":"Handbook of Venoms and Toxins of Reptiles","author":"Guti\u00e9rrez JM","year":"2009"},{"key":"bibr-EBM-1104-RM-125C3","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.02.012"},{"key":"bibr-EBM-1104-RM-125C4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2008.06466.x"},{"key":"bibr-EBM-1104-RM-125C5","first-page":"95","volume-title":"Handbook of Venoms and Toxins of Reptiles","author":"Fox JW","year":"2009"},{"key":"bibr-EBM-1104-RM-125C6","doi-asserted-by":"publisher","DOI":"10.1023\/A:1014465611487"},{"key":"bibr-EBM-1104-RM-125C7","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324475"},{"key":"bibr-EBM-1104-RM-125C8","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2003.12.008"},{"key":"bibr-EBM-1104-RM-125C9","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.07.010"},{"key":"bibr-EBM-1104-RM-125C10","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(00)00089-1"},{"key":"bibr-EBM-1104-RM-125C11","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2006.12.014"},{"key":"bibr-EBM-1104-RM-125C12","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2010.06.005"},{"key":"bibr-EBM-1104-RM-125C13","doi-asserted-by":"publisher","DOI":"10.1016\/j.crvi.2006.10.001"},{"key":"bibr-EBM-1104-RM-125C14","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2010.07.006"},{"key":"bibr-EBM-1104-RM-125C15","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762005000900031"},{"key":"bibr-EBM-1104-RM-125C16","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2009.07.025"},{"key":"bibr-EBM-1104-RM-125C17","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(83)90137-X"},{"key":"bibr-EBM-1104-RM-125C18","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90442-7"},{"key":"bibr-EBM-1104-RM-125C19","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2007.01.017"},{"key":"bibr-EBM-1104-RM-125C20","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-0886-7_10"},{"key":"bibr-EBM-1104-RM-125C21","doi-asserted-by":"publisher","DOI":"10.1080\/096293500411569"},{"key":"bibr-EBM-1104-RM-125C22","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(73)90054-X"},{"key":"bibr-EBM-1104-RM-125C24","doi-asserted-by":"publisher","DOI":"10.1590\/S0101-81752007000200019"},{"key":"bibr-EBM-1104-RM-125C25","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.05.030"},{"key":"bibr-EBM-1104-RM-125C26","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.06.002"},{"key":"bibr-EBM-1104-RM-125C27","doi-asserted-by":"publisher","DOI":"10.1081\/TXR-120004744"},{"key":"bibr-EBM-1104-RM-125C28","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.02.013"},{"key":"bibr-EBM-1104-RM-125C29","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.06.019"},{"key":"bibr-EBM-1104-RM-125C30","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90279-8"},{"key":"bibr-EBM-1104-RM-125C31","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.02.001"}],"container-title":["Experimental Biology and Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/ebm.2011.011125","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1258\/ebm.2011.011125","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/ebm.2011.011125","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T17:56:36Z","timestamp":1777571796000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ebm-journal.org\/journals\/experimental-biology-and-medicine\/articles\/10.1258\/ebm.2011.011125\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10]]},"references-count":30,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2011,10]]}},"alternative-id":["10.1258\/ebm.2011.011125"],"URL":"https:\/\/doi.org\/10.1258\/ebm.2011.011125","relation":{},"ISSN":["1535-3702","1535-3699"],"issn-type":[{"value":"1535-3702","type":"print"},{"value":"1535-3699","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10]]}}}