{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T15:36:45Z","timestamp":1778600205308,"version":"3.51.4"},"reference-count":36,"publisher":"Frontiers Media SA","issue":"8","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Exp Biol Med (Maywood)"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>Diabetic nephropathy (DN) remains the most common cause of end-stage renal disease (ESRD) as the burden of diabetes increases worldwide. To find improved intervention strategies for this disease, it is necessary to investigate the molecular mechanisms involved. To obtain more insight into processes that lead to DN, mRNA expression profiles of diabetic and normal glomeruli from rat kidneys were compared. Rats were divided into a control group and a DN group randomly. The DN group was injected with streptozotocin. Fasting blood glucose (FBG) and weight were measured monthly. On the 12th week, blood samples were collected and analyzed for plasma creatinine and blood urea nitrogen (BUN). Glomeruli were isolated and Illumina Rat Ref-12 V1.0 Expression Beadchip gene array was performed. Quantitative realtime polymerase chain reaction (Q-RT-PCR) was used to confirm the results of gene array for a selected number of genes. We found FBG, 24-h urinary albumin, serum creatinine and BUN were significantly increased, while urinary creatinine and body weight were significantly decreased in the DN group. Glomeruli from the DN group had 624 genes with differential expression. DAVID (Database for Annotation, Visualization and integrated Discovery) analysis showed that the three most enriched terms were \u2018cytosol\u2019 (GO:0005829), \u2018translational elongation\u2019 (GO:0006414) and \u2018mitochondion\u2019 (GO:0005739). Those genes could be mapped to eight pathways. The most common type of enriched pathway was related to \u2018extracellular matrix (ECM)-receptor interaction\u2019. Other pathways included those for \u2018ribosome\u2019, \u2018focal adhesion\u2019, \u2018oxidative phosphorylation\u2019, \u2018transforming growth factor (TGF)-beta signaling pathway\u2019, \u2018Parkinson's disease\u2019, \u2018Alzheimer's disease\u2019 and \u2018renin\u2013angiotensin system\u2019. Q-RT-PCR verified that Atp5b (F1-ATPase beta subunit), Col1a1 (collagen type 1 alpha 1), Cox6c (cytochrome c oxidase subunit VIc), Ndufs3 (NADH dehydrogenase [ubiquinone] Fe-S protein 3) and Tgfb1 (transforming growth factor \u03b21) were significantly up-regulated in the DN group. The expressions of NDUFS3 and TGF- \u03b21 in DN rats were increased. Our findings suggested that the oxidative phosphorylation pathway, ECM-receptor interaction, TGF- \u03b2 pathway and renin\u2013angiotensin system may be involved in the development of DN.<\/jats:p>","DOI":"10.1258\/ebm.2012.012032","type":"journal-article","created":{"date-parts":[[2012,8,18]],"date-time":"2012-08-18T00:25:14Z","timestamp":1345249514000},"page":"903-911","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Gene expression profiling in glomeruli of diabetic nephropathy rat"],"prefix":"10.3389","volume":"237","author":[{"given":"Qian","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Endocrinology, Ministry of Health, Department of Endocrinology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No. 1 Shuaifu Garden, Beijing 100730, 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