{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:00:44Z","timestamp":1791046844907,"version":"4.1.0"},"reference-count":25,"publisher":"Frontiers Media SA","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Exp Biol Med (Maywood)"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p>Cholangiocarcinoma (CCA) affects the intra- and extrahepatic bile ducts and is commonly burdened by a late presentation and resulting high mortality rate. Accordingly, finding non-invasive biomarkers with adequate diagnostic\/prognostic values is a priority in high-risk populations. In this study, we analyzed proteomes of serum samples from six CCA cases and ten healthy subjects using two-dimensional polyacrylamide gel electrophoresis to identify CCA-associated spots. Thirty-six CCA-associated proteins found in sera were identified by mass spectrometry. \u03b11 \u03b2-Glycoprotein (A1BG) and afamin (AFM) were detected consistently at different degrees in CCA sera compared with controls and were validated for their diagnostic and prognostic potential in a larger cohort of 64 patients with CCA, 4 with benign biliary diseases and 20 healthy subjects and compared between pre- and postsurgery serum samples from 26 CCA patients to ascertain a prognostic correlation. A single blot test developed to assess the serum A1BG\/AFM ratio could detect CCA cases with 87.5% specificity, 84.4% sensitivity and the levels were significantly higher in CCA compared with controls. A high level of postoperative serum A1BG\/AFM ratio was associated with worse outcomes and the infiltration of resection margins. The A1BG\/AFM ratio may constitute a novel non-invasive candidate marker to diagnose CCA and its outcomes with high specificity and sensitivity. Prospective studies are awaited to demonstrate the clinical value of this observation.<\/jats:p>","DOI":"10.1258\/ebm.2012.012215","type":"journal-article","created":{"date-parts":[[2012,10,26]],"date-time":"2012-10-26T22:44:05Z","timestamp":1351291445000},"page":"1142-1149","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":22,"title":["Serum\n                    <i>\u03b1<\/i>\n                    <sub>1<\/sub>\n                    <i>\u03b2<\/i>\n                    -glycoprotein and afamin ratio as potential diagnostic and prognostic markers in cholangiocarcinoma"],"prefix":"10.3389","volume":"237","author":[{"given":"Arthit","family":"Tolek","sequence":"first","affiliation":[{"name":"Department of Biochemistry"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaisiri","family":"Wongkham","sequence":"additional","affiliation":[{"name":"Department of Biochemistry"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siriporn","family":"Proungvitaya","sequence":"additional","affiliation":[{"name":"Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine"},{"name":"Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atit","family":"Silsirivanit","sequence":"additional","affiliation":[{"name":"Department of Biochemistry"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sittiruk","family":"Roytrakul","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology, NSTDA, Pathumthani 12120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narong","family":"Khuntikeo","sequence":"additional","affiliation":[{"name":"Department of Surgery"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sopit","family":"Wongkham","sequence":"additional","affiliation":[{"name":"Department of Biochemistry"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2012,10,1]]},"reference":[{"key":"bibr-EBM-1206-RM-215C1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.25087"},{"key":"bibr-EBM-1206-RM-215C2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-828889"},{"key":"bibr-EBM-1206-RM-215C3","first-page":"83","volume":"9","author":"Khuntikeo N","year":"2008","journal-title":"Asian Pac J Cancer Prev"},{"key":"bibr-EBM-1206-RM-215C4","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401287"},{"key":"bibr-EBM-1206-RM-215C5","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200700246"},{"key":"bibr-EBM-1206-RM-215C6","doi-asserted-by":"publisher","DOI":"10.1021\/pr800570s"},{"key":"bibr-EBM-1206-RM-215C7","doi-asserted-by":"publisher","DOI":"10.1186\/1477-5956-9-21"},{"key":"bibr-EBM-1206-RM-215C8","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-3656-4"},{"key":"bibr-EBM-1206-RM-215C9","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"bibr-EBM-1206-RM-215C10","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"bibr-EBM-1206-RM-215C11","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1997.2061"},{"key":"bibr-EBM-1206-RM-215C12","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)61165-0"},{"key":"bibr-EBM-1206-RM-215C13","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-200112000-00008"},{"key":"bibr-EBM-1206-RM-215C14","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-8-241"},{"key":"bibr-EBM-1206-RM-215C15","first-page":"315","volume":"29","author":"Yoon SY","year":"2006","journal-title":"Int J Oncol"},{"key":"bibr-EBM-1206-RM-215C16","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32429-8"},{"key":"bibr-EBM-1206-RM-215C17","doi-asserted-by":"publisher","DOI":"10.1021\/bi026513v"},{"key":"bibr-EBM-1206-RM-215C18","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-0747"},{"key":"bibr-EBM-1206-RM-215C19","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-08-0653"},{"key":"bibr-EBM-1206-RM-215C20","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(00)00589-9"},{"key":"bibr-EBM-1206-RM-215C21","doi-asserted-by":"publisher","DOI":"10.3802\/jgo.2008.19.3.173"},{"key":"bibr-EBM-1206-RM-215C22","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-147-7-200710020-00003"},{"key":"bibr-EBM-1206-RM-215C23","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2011.06.011"},{"key":"bibr-EBM-1206-RM-215C24","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)61965-4"},{"key":"bibr-EBM-1206-RM-215C25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2008.08.024"}],"container-title":["Experimental Biology and Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/ebm.2012.012215","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1258\/ebm.2012.012215","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/ebm.2012.012215","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T17:57:14Z","timestamp":1777571834000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ebm-journal.org\/journals\/experimental-biology-and-medicine\/articles\/10.1258\/ebm.2012.012215\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10]]},"references-count":25,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2012,10]]}},"alternative-id":["10.1258\/ebm.2012.012215"],"URL":"https:\/\/doi.org\/10.1258\/ebm.2012.012215","relation":{},"ISSN":["1535-3702","1535-3699"],"issn-type":[{"value":"1535-3702","type":"print"},{"value":"1535-3699","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10]]}}}