{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:11:55Z","timestamp":1760983915654,"version":"3.38.0"},"reference-count":11,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lab Anim"],"published-print":{"date-parts":[[2011,4]]},"abstract":" The use of dried blood spots (DBS) in preclinical studies has seen an enormous increase over the past two years. Despite its positive impact on the 3Rs (reduce, replace and refine), its uptake in exploratory drug discovery has been limited due mainly to protracted method development time in bioanalysis but also the need for small volumes (<20 \u03bcL) to be sampled manually. Automatic blood sampling technology such as the DiLab\u00ae<\/jats:sup> AccuSampler\u00ae<\/jats:sup> is widely used in drug discovery to facilitate exploratory rodent-based pharmacokinetic and pharmacokinetic\/pharmacodynamic studies with minimal animal handling. Propranolol was orally administered to a Han-Wistar rat attached to either a standard DiLab\u00ae<\/jats:sup> AccuSampler\u00ae<\/jats:sup> or a retrofitted unit designed to directly collect the DBS samples. In all, 50 or 20 \u03bcL blood samples were then collected via the standard or retrofitted unit, respectively, at six timepoints over a 7 h period. After drying and storage the DBS samples were analysed for propranolol via liquid chromatography-mass spectrometry. In this report we demonstrate that a standard DiLab\u00ae<\/jats:sup> AccuSampler\u00ae<\/jats:sup> can be easily retrofitted to facilitate automatic dried blood spot sampling and that time\u2013concentration data generated from these samples are equivalent to that from manually spotted samples. <\/jats:p>","DOI":"10.1258\/la.2010.010155","type":"journal-article","created":{"date-parts":[[2011,2,26]],"date-time":"2011-02-26T02:22:11Z","timestamp":1298686931000},"page":"124-126","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["Utilization of dried blood spots within drug discovery: modification of a standard DiLab\u00ae<\/sup> AccuSampler\u00ae<\/sup> to facilitate automatic dried blood spot sampling"],"prefix":"10.1177","volume":"45","author":[{"given":"G T","family":"Clark","sequence":"first","affiliation":[{"name":"Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Worldwide Research and Development, Sandwich, Kent CT13 9NJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Giddens","sequence":"additional","affiliation":[{"name":"Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Worldwide Research and Development, Sandwich, Kent CT13 9NJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Burrows","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine, Pfizer Worldwide Research and Development, Sandwich, Kent CT13 9NJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Strand","sequence":"additional","affiliation":[{"name":"DiLab (part of CMA group), Skarpskyttev\u00e4gen 3, SE-226 42 Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2011,4,1]]},"reference":[{"key":"bibr-LA-10-155C1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1367"},{"key":"bibr-LA-10-155C2","doi-asserted-by":"publisher","DOI":"10.4155\/bio.10.91"},{"key":"bibr-LA-10-155C3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2008.05.025"},{"key":"bibr-LA-10-155C4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.06.018"},{"key":"bibr-LA-10-155C5","doi-asserted-by":"publisher","DOI":"10.4155\/bio.10.94"},{"key":"bibr-LA-10-155C6","first-page":"435","volume-title":"In Vivo","author":"Siswanto H","year":"2008"},{"key":"bibr-LA-10-155C7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2006.00029.x"},{"key":"bibr-LA-10-155C8","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1800145"},{"key":"bibr-LA-10-155C9","doi-asserted-by":"publisher","DOI":"10.1097\/01.ftd.0000170879.18139.40"},{"key":"bibr-LA-10-155C10","doi-asserted-by":"publisher","DOI":"10.1002\/bdd.2510070206"},{"key":"bibr-LA-10-155C11","unstructured":"KwonY. Handbook of Essential Pharmacokinetics, Pharmacodynamics and Drug Metabolism for Industrial Scientists. New York: Kluwer Academic\/Plenum Publishers, 2001:229\u201340"}],"container-title":["Laboratory Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/la.2010.010155","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1258\/la.2010.010155","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/la.2010.010155","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,2]],"date-time":"2025-03-02T21:10:13Z","timestamp":1740949813000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1258\/la.2010.010155"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,4]]},"references-count":11,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,4]]}},"alternative-id":["10.1258\/la.2010.010155"],"URL":"https:\/\/doi.org\/10.1258\/la.2010.010155","relation":{},"ISSN":["0023-6772","1758-1117"],"issn-type":[{"type":"print","value":"0023-6772"},{"type":"electronic","value":"1758-1117"}],"subject":[],"published":{"date-parts":[[2011,4]]}}}