{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:49:13Z","timestamp":1761511753467,"version":"3.33.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2002,8,1]],"date-time":"2002-08-01T00:00:00Z","timestamp":1028160000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J STD AIDS"],"published-print":{"date-parts":[[2002,8,1]]},"abstract":"<jats:p> The sensitivity of two urine pool sizes versus individual testing, to detect Chlamydia trachomatis urogenital infection, was evaluated using the Gen-Probe AMP-CT assay. <\/jats:p><jats:p> Thirty-three (33) known polymerase chain reaction (PCR) positive urine specimens were combined with 231 fresh first-catch urine (FCU) samples in 33 groups of four and 33 groups of eight, to make up 4X and 8X pooled samples, respectively. Gen-Probe AMP-CT assay was performed on pools as well as on individual samples at the same time. For the discrepant cases, the known positive samples were diluted 1:4 and 1:8 using the manufacturer's dilution buffer and were retested. Additional positive specimens found among fresh FCU samples were also tested by the Amplicor-PCR assay to confirm their positivity. The sensitivities of 8X pooling, 4X pooling and individual testing were 86.5%, 94.3% and 91.9%, respectively. <\/jats:p><jats:p> The Gen-Probe AMP-CT assay applied to a 4X urine pooling model was highly sensitive and may be useful for a population based screening programme. <\/jats:p>","DOI":"10.1258\/095646202760159648","type":"journal-article","created":{"date-parts":[[2002,10,16]],"date-time":"2002-10-16T09:48:30Z","timestamp":1034761710000},"page":"540-542","source":"Crossref","is-referenced-by-count":5,"title":["Sensitivity evaluation of the Gen-Probe AMP-CT assay by pooling urine                samples for the screening of <i>Chlamydia trachomatis<\/i> urogenital                infection"],"prefix":"10.1177","volume":"13","author":[{"given":"J P","family":"Gomes","sequence":"first","affiliation":[{"name":"Bacteriology Department, National Institute of Health, Lisbon,\r                  Portugal"}]},{"given":"S","family":"Viegas","sequence":"additional","affiliation":[{"name":"Bacteriology Department, National Institute of Health, Lisbon,\r                  Portugal"}]},{"given":"A","family":"Paulino","sequence":"additional","affiliation":[{"name":"Bacteriology Department, National Institute of Health, Lisbon,\r                  Portugal"}]},{"given":"M A","family":"Catry","sequence":"additional","affiliation":[{"name":"Bacteriology Department, National Institute of Health, Lisbon,\r                  Portugal"}]}],"member":"179","published-online":{"date-parts":[[2002,8,1]]},"container-title":["International Journal of STD &amp; AIDS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/095646202760159648","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1258\/095646202760159648","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T13:09:46Z","timestamp":1738069786000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1258\/095646202760159648"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,8,1]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2002,8,1]]}},"alternative-id":["10.1258\/095646202760159648"],"URL":"https:\/\/doi.org\/10.1258\/095646202760159648","relation":{},"ISSN":["0956-4624","1758-1052"],"issn-type":[{"type":"print","value":"0956-4624"},{"type":"electronic","value":"1758-1052"}],"subject":[],"published":{"date-parts":[[2002,8,1]]}}}