{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T17:55:37Z","timestamp":1791482137905,"version":"4.3.4"},"reference-count":25,"publisher":"Cold Spring Harbor Laboratory","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["RNA"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p>The mRNA processing body (P-body) is a cellular structure that regulates gene expression by degrading cytoplasmic mRNA. The objective of this study was to identify and characterize novel components of the mammalian P-body. Approximately 5% of patients with the autoimmune disease primary biliary cirrhosis have antibodies directed against this structure. Serum from one of these patients was used to identify a cDNA encoding Ge-1, a 1401-amino-acid protein. Ge-1 contains an N-terminal WD40 motif and C-terminal domains characterized by a repeating \u03c8(X<jats:sub>2\u20133<\/jats:sub>) motif. Ge-1 co-localized with previously identified P-body components, including proteins involved in mRNA decapping (DCP1a and DCP2) and the autoantigen GW182. The Ge-1 C-terminal domain was necessary and sufficient to target the protein to P-bodies. Following exposure of cells to oxidative stress, Ge-1-containing P-bodies were found adjacent to TIA-containing stress granules. During the recovery period, TIA returned to the nucleus while Ge-1-containing P-bodies localized to the perinuclear region. siRNA-mediated knock-down of Ge-1 resulted in loss of P-bodies containing Ge-1, DCP1a, and DCP2. In contrast, Ge-1-containing P-bodies persisted despite knock-down of DCP2. Taken together, the results of this study show that Ge-1 is a central component of P-bodies and suggest that Ge-1 may act prior to the 5<jats:sup>\u2032<\/jats:sup>-decapping step in mRNA degradation.<\/jats:p>","DOI":"10.1261\/rna.2142405","type":"journal-article","created":{"date-parts":[[2005,11,28]],"date-time":"2005-11-28T20:18:36Z","timestamp":1133209116000},"page":"1795-1802","source":"Crossref","is-referenced-by-count":177,"title":["Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body"],"prefix":"10.1261","volume":"11","author":[{"given":"JIANG HONG","family":"YU","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WEI-HONG","family":"YANG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TOD","family":"GULICK","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KENNETH D.","family":"BLOCH","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DONALD B.","family":"BLOCH","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2005,11,28]]},"reference":[{"key":"2021112105402312000_11.12.1795.1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2340405"},{"key":"2021112105402312000_11.12.1795.2","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1006\/clin.1994.1156","volume":"72","year":"1994","journal-title":"Clin. 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