{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T17:55:38Z","timestamp":1791482138733,"version":"4.3.4"},"reference-count":30,"publisher":"Cold Spring Harbor Laboratory","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["RNA"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:p>The mRNA processing body (P-body) is a cellular structure that has an important role in mRNA degradation. P-bodies have also been implicated in RNAi-mediated post-transcriptional gene silencing. The objective of this study was to identify and characterize novel components of the mammalian P-body. Approximately 5% of patients with the autoimmune disease primary biliary cirrhosis have antibodies directed against this structure. Serum from one of these patients was used to identify a cDNA encoding RAP55, a 463\u2013amino acid protein. RAP55 colocalized with previously identified P-body components DCP1a and Ge-1. RAP55 contains an N-terminal Sm-like domain and two C-terminal RGG-rich domains separated by an FDF motif. The two RGG domains and the FDF domain were necessary and sufficient to target the protein to P-bodies. A fragment of RAP55 consisting of the FDF and the second RGG domains did not localize to P-bodies, but was able to displace other P-body components from this structure. After cells were subjected to arsenite-induced stress, RAP55 was detected in TIA-containing stress granules. The second RGG domain was necessary and sufficient for stress granule localization. siRNA-mediated knock-down of RAP55 resulted in loss of P-bodies, suggesting that RAP55 acts prior to the 5\u2032-decapping step in mRNA degradation. The results of this study show that RAP55 is a component of P-bodies in cells at rest and localizes in stress granules in arsenite-treated cells. RAP55 may serve to shuttle mRNAs between P-bodies and stress granules.<\/jats:p>","DOI":"10.1261\/rna.2302706","type":"journal-article","created":{"date-parts":[[2006,2,16]],"date-time":"2006-02-16T20:43:59Z","timestamp":1140122639000},"page":"547-554","source":"Crossref","is-referenced-by-count":161,"title":["RNA-associated protein 55 (RAP55) localizes to mRNA processing bodies and stress granules"],"prefix":"10.1261","volume":"12","author":[{"given":"WEI-HONG","family":"YANG","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JIANG HONG","family":"YU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TOD","family":"GULICK","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KENNETH D.","family":"BLOCH","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DONALD B.","family":"BLOCH","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2006,2,16]]},"reference":[{"key":"2021112105404496000_12.4.547.1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-5-45"},{"key":"2021112105404496000_12.4.547.2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2340405"},{"key":"2021112105404496000_12.4.547.3","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200506124"},{"key":"2021112105404496000_12.4.547.4","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1006\/clin.1994.1156","volume":"72","year":"1994","journal-title":"Clin. 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