{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:13:28Z","timestamp":1790900008249,"version":"4.1.0"},"reference-count":64,"publisher":"Cold Spring Harbor Laboratory","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["RNA"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p>Humans have two near identical copies of the <jats:italic>survival of motor neuron<\/jats:italic> (<jats:italic>SMN<\/jats:italic>) gene, <jats:italic>SMN1<\/jats:italic> and <jats:italic>SMN2<\/jats:italic>. In spinal muscular atrophy (SMA), <jats:italic>SMN2<\/jats:italic> is not able to compensate for the loss of <jats:italic>SMN1<\/jats:italic> due to an inhibitory mutation at position 6 (C6U mutation in transcript) of exon 7. We have recently shown that C6U creates an <jats:underline>ex<\/jats:underline>tended <jats:underline>in<\/jats:underline>hibitory <jats:underline>c<\/jats:underline>ontex<jats:underline>t<\/jats:underline> (Exinct) that causes skipping of exon 7 in <jats:italic>SMN2<\/jats:italic>. Previous studies have shown that an exonic splicing enhancer associated with Tra2 (Tra2-ESE) is required for exon 7 inclusion in both <jats:italic>SMN1<\/jats:italic> and <jats:italic>SMN2<\/jats:italic>. Here we describe the method of in vivo selection that determined the position-specific role of wild-type nucleotides within the entire exon 7. Our results confirmed the existence of Exinct and revealed the presence of an additional inhibitory tract (3\u2032-Cluster) near the 3\u2032-end of exon 7. We also demonstrate that a single nucleotide substitution at the last position of exon 7 improves the 5\u2032 splice site (ss) such that the presence of inhibitory elements (Exinct as well as the 3\u2032-Cluster) and the absence of Tra2-ESE no longer determined exon 7 usage. Our results suggest that the evolutionary conserved weak 5\u2032 ss may serve as a mechanism to regulate exon 7 splicing under different physiological contexts. This is the first report in which a functional selection method has been applied to analyze the entire exon. This method offers unparallel advantage for determining the relative strength of splice sites, as well as for identifying the novel exonic <jats:italic>cis<\/jats:italic>-elements.<\/jats:p>","DOI":"10.1261\/rna.7580704","type":"journal-article","created":{"date-parts":[[2004,7,23]],"date-time":"2004-07-23T01:54:47Z","timestamp":1090547687000},"page":"1291-1305","source":"Crossref","is-referenced-by-count":121,"title":["In vivo selection reveals combinatorial controls that define a critical exon in the spinal muscular atrophy genes"],"prefix":"10.1261","volume":"10","author":[{"given":"NATALIA N.","family":"SINGH","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ELLIOT J.","family":"ANDROPHY","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RAVINDRA N.","family":"SINGH","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2004,7,22]]},"reference":[{"key":"2021112105345726000_10.8.1291.1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.24.2841"},{"key":"2021112105345726000_10.8.1291.2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90527-6"},{"key":"2021112105345726000_10.8.1291.3","doi-asserted-by":"crossref","first-page":"7559","DOI":"10.1128\/jvi.69.12.7559-7569.1995","volume":"69","year":"1995","journal-title":"J. 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