{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:43:46Z","timestamp":1778028226861,"version":"3.51.4"},"reference-count":90,"publisher":"Cold Spring Harbor Laboratory","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["RNA"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:p>Studies over the past years indicate that there is extensive coupling between nuclear export of mRNA and pre-mRNA processing. Here, we visualized the distribution of exon junction complex (EJC) proteins and RNA export factors relative to sites of abundant pre-mRNA synthesis in the nucleus. We analyzed both HeLa cells infected with adenovirus and murine erythroleukemia (MEL) cells stably transfected with the human \u03b2-globin gene. Using in situ hybridization and confocal microscopy, we observe accumulation of EJC proteins (REF\/Aly, Y14, SRm160, UAP56, RNPS1, and Magoh) and core spliceosome components (U snRNPs) at sites of transcription. This suggests that EJC proteins bind stably to pre-mRNA cotranscriptionally. No concentration of the export factors NXF1\/TAP, p15, and Dbp5 was detected on nascent transcripts, arguing that in mammalian cells these proteins bind the mRNA shortly before or after release from the sites of transcription. These results also suggest that binding of EJC proteins to the mRNA is not sufficient to recruit TAP-p15, consistent with recent findings showing that the EJC does not play a crucial role in mRNA export. Contrasting to the results obtained in MEL cells expressing normal human \u03b2-globin transcripts, mutant pre-mRNAs defective in splicing and 3\u2032end processing do not colocalize with SRm160, REF, UAP56, or Sm proteins. This shows that the accumulation of EJC proteins at transcription sites requires efficient processing of the nascent pre-mRNAs, arguing that transcription per se is not sufficient for the stable assembly of the EJC.<\/jats:p>","DOI":"10.1261\/rna.5258504","type":"journal-article","created":{"date-parts":[[2004,3,22]],"date-time":"2004-03-22T22:13:58Z","timestamp":1079993638000},"page":"622-633","source":"Crossref","is-referenced-by-count":75,"title":["In vivo recruitment of exon junction complex proteins to transcription sites in mammalian cell nuclei"],"prefix":"10.1261","volume":"10","author":[{"given":"NO\u00c9LIA","family":"CUST\u00d3DIO","sequence":"first","affiliation":[]},{"given":"C\u00c9LIA","family":"CARVALHO","sequence":"additional","affiliation":[]},{"given":"IN\u00caS","family":"CONDADO","sequence":"additional","affiliation":[]},{"given":"MICHAEL","family":"ANTONIOU","sequence":"additional","affiliation":[]},{"given":"BENJAMIN J.","family":"BLENCOWE","sequence":"additional","affiliation":[]},{"given":"MARIA","family":"CARMO-FONSECA","sequence":"additional","affiliation":[]}],"member":"246","published-online":{"date-parts":[[2004,3,22]]},"reference":[{"key":"2021112105343642000_10.4.622.1","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1101\/gad.14.11.1308","volume":"14","year":"2000","journal-title":"Genes & Dev."},{"key":"2021112105343642000_10.4.622.2","doi-asserted-by":"crossref","unstructured":"Antoniou, A. 1991. Induction of erythroid-specific expression in murine erythroleukemia (MEL) cells. In Methods in molecular biology: Gene transfer and expression protocols. (ed. E.J. Murray), pp. 421\u2013434. The Human Press, Inc., Clinfton, N.J.","DOI":"10.1385\/0-89603-178-0:421"},{"key":"2021112105343642000_10.4.622.3","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.3.721"},{"key":"2021112105343642000_10.4.622.4","doi-asserted-by":"publisher","DOI":"10.1017\/S1355838200991994"},{"key":"2021112105343642000_10.4.622.5","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90182-1"},{"key":"2021112105343642000_10.4.622.6","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.133.5.929"},{"key":"2021112105343642000_10.4.622.7","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2.6.754"},{"key":"2021112105343642000_10.4.622.8","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.7.996"},{"key":"2021112105343642000_10.4.622.9","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1128\/jvi.69.1.281-290.1995","volume":"69","year":"1995","journal-title":"J. Virol."},{"key":"2021112105343642000_10.4.622.10","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.135.2.303"},{"key":"2021112105343642000_10.4.622.11","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1002\/j.1460-2075.1990.tb08100.x","volume":"9","year":"1990","journal-title":"EMBO J."},{"key":"2021112105343642000_10.4.622.12","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00268"},{"key":"2021112105343642000_10.4.622.13","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.10.2855"},{"key":"2021112105343642000_10.4.622.14","doi-asserted-by":"publisher","DOI":"10.1038\/nrm760"},{"key":"2021112105343642000_10.4.622.15","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E02-09-0602"},{"key":"2021112105343642000_10.4.622.16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006454200"},{"key":"2021112105343642000_10.4.622.17","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.5.1654"},{"key":"2021112105343642000_10.4.622.18","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.14.1864"},{"key":"2021112105343642000_10.4.622.19","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0065-3527(08)60264-X","volume":"31","year":"1986","journal-title":"Adv. Virus Res."},{"key":"2021112105343642000_10.4.622.20","first-page":"1739","volume":"10","year":"1995","journal-title":"Oncogene"},{"key":"2021112105343642000_10.4.622.21","doi-asserted-by":"publisher","DOI":"10.1038\/nsb926"},{"key":"2021112105343642000_10.4.622.22","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.137.5.975"},{"key":"2021112105343642000_10.4.622.23","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(03)00251-9"},{"key":"2021112105343642000_10.4.622.24","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200207128"},{"key":"2021112105343642000_10.4.622.25","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00532-2"},{"key":"2021112105343642000_10.4.622.26","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90382-Z"},{"key":"2021112105343642000_10.4.622.27","doi-asserted-by":"crossref","first-page":"2560","DOI":"10.4049\/jimmunol.143.8.2560","volume":"143","year":"1989","journal-title":"J. Immunol."},{"key":"2021112105343642000_10.4.622.28","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00508-5"},{"key":"2021112105343642000_10.4.622.29","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.23.8996-9008.2000"},{"key":"2021112105343642000_10.4.622.30","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.20.5778"},{"key":"2021112105343642000_10.4.622.31","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.133.4.719"},{"key":"2021112105343642000_10.4.622.32","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1128\/MCB.16.4.1534","volume":"16","year":"1996","journal-title":"Mol. Cell. Biol."},{"key":"2021112105343642000_10.4.622.33","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00089-3"},{"key":"2021112105343642000_10.4.622.34","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00273"},{"key":"2021112105343642000_10.4.622.35","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90159-N"},{"key":"2021112105343642000_10.4.622.36","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)00065-4"},{"key":"2021112105343642000_10.4.622.37","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.22.6424"},{"key":"2021112105343642000_10.4.622.38","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)00840-0"},{"key":"2021112105343642000_10.4.622.39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1016-8478(23)17055-5","volume":"12","year":"2001","journal-title":"Mol. Cells"},{"key":"2021112105343642000_10.4.622.40","doi-asserted-by":"publisher","DOI":"10.1126\/science.1062829"},{"key":"2021112105343642000_10.4.622.41","doi-asserted-by":"publisher","DOI":"10.1101\/gad.851301"},{"key":"2021112105343642000_10.4.622.42","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00328-2"},{"key":"2021112105343642000_10.4.622.43","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.24.6860"},{"key":"2021112105343642000_10.4.622.44","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1101\/gad.14.9.1098","volume":"14","year":"2000","journal-title":"Genes & Dev."},{"key":"2021112105343642000_10.4.622.45","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kve245"},{"key":"2021112105343642000_10.4.622.46","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.17.4987"},{"key":"2021112105343642000_10.4.622.47","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1032902"},{"key":"2021112105343642000_10.4.622.48","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00134-X"},{"key":"2021112105343642000_10.4.622.49","doi-asserted-by":"publisher","DOI":"10.1101\/gad.892401"},{"key":"2021112105343642000_10.4.622.50","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf345"},{"key":"2021112105343642000_10.4.622.51","first-page":"6059","volume":"10","year":"1990","journal-title":"Mol. Cell. Biol."},{"key":"2021112105343642000_10.4.622.52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.5.2737"},{"key":"2021112105343642000_10.4.622.53","doi-asserted-by":"publisher","DOI":"10.1101\/gad.852101"},{"key":"2021112105343642000_10.4.622.54","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1111\/j.1432-1033.1977.tb11618.x","volume":"76","year":"1977","journal-title":"Eur. J. Biochem."},{"key":"2021112105343642000_10.4.622.55","doi-asserted-by":"publisher","DOI":"10.1261\/rna.5420503"},{"key":"2021112105343642000_10.4.622.56","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1242\/jcs.111.11.1495","volume":"111","year":"1998","journal-title":"J. Cell. Sci."},{"key":"2021112105343642000_10.4.622.57","doi-asserted-by":"publisher","DOI":"10.1261\/rna.5260303"},{"key":"2021112105343642000_10.4.622.58","doi-asserted-by":"publisher","DOI":"10.1038\/35098106"},{"key":"2021112105343642000_10.4.622.59","doi-asserted-by":"publisher","DOI":"10.1126\/science.1062786"},{"key":"2021112105343642000_10.4.622.60","doi-asserted-by":"publisher","DOI":"10.1261\/rna.5480803"},{"key":"2021112105343642000_10.4.622.61","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.16.4560"},{"key":"2021112105343642000_10.4.622.62","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.24.3306"},{"key":"2021112105343642000_10.4.622.63","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.1.148-160.2002"},{"key":"2021112105343642000_10.4.622.64","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.2.309"},{"key":"2021112105343642000_10.4.622.65","doi-asserted-by":"crossref","first-page":"2886","DOI":"10.1101\/gad.927001","volume":"15","year":"2001","journal-title":"Genes & Dev."},{"key":"2021112105343642000_10.4.622.66","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.24.3279"},{"key":"2021112105343642000_10.4.622.67","doi-asserted-by":"publisher","DOI":"10.1261\/rna.5250403"},{"key":"2021112105343642000_10.4.622.68","first-page":"1","volume":"14","year":"1975","journal-title":"Virol. Monogr."},{"key":"2021112105343642000_10.4.622.69","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1002\/rmv.1980050405","volume":"5","year":"1995","journal-title":"Rev. Med. Virol."},{"key":"2021112105343642000_10.4.622.70","doi-asserted-by":"crossref","first-page":"5075","DOI":"10.1002\/j.1460-2075.1994.tb06837.x","volume":"13","year":"1994","journal-title":"EMBO J."},{"key":"2021112105343642000_10.4.622.71","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00627-X"},{"key":"2021112105343642000_10.4.622.72","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1030602"},{"key":"2021112105343642000_10.4.622.73","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.031586198"},{"key":"2021112105343642000_10.4.622.74","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.15.4332"},{"key":"2021112105343642000_10.4.622.75","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.9.2663"},{"key":"2021112105343642000_10.4.622.76","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.20.5761"},{"key":"2021112105343642000_10.4.622.77","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.3.410"},{"key":"2021112105343642000_10.4.622.78","doi-asserted-by":"publisher","DOI":"10.1038\/nature746"},{"key":"2021112105343642000_10.4.622.79","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(03)00106-5"},{"key":"2021112105343642000_10.4.622.80","doi-asserted-by":"publisher","DOI":"10.1017\/S1355838200000078"},{"key":"2021112105343642000_10.4.622.81","doi-asserted-by":"publisher","DOI":"10.1038\/ng0295-184"},{"key":"2021112105343642000_10.4.622.82","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.9.2651"},{"key":"2021112105343642000_10.4.622.83","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80369-X"},{"key":"2021112105343642000_10.4.622.84","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1934877100"},{"key":"2021112105343642000_10.4.622.85","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.113.3.465"},{"key":"2021112105343642000_10.4.622.86","doi-asserted-by":"publisher","DOI":"10.1126\/science.8446901"},{"key":"2021112105343642000_10.4.622.87","doi-asserted-by":"publisher","DOI":"10.1038\/372809a0"},{"key":"2021112105343642000_10.4.622.88","doi-asserted-by":"publisher","DOI":"10.1101\/gad.851701"},{"key":"2021112105343642000_10.4.622.89","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.5.1177"},{"key":"2021112105343642000_10.4.622.90","doi-asserted-by":"publisher","DOI":"10.1038\/35030160"}],"container-title":["RNA"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1261\/rna.5258504","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T19:20:20Z","timestamp":1705087220000},"score":1,"resource":{"primary":{"URL":"http:\/\/rnajournal.cshlp.org\/lookup\/doi\/10.1261\/rna.5258504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,3,22]]},"references-count":90,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,4]]}},"alternative-id":["10.1261\/rna.5258504"],"URL":"https:\/\/doi.org\/10.1261\/rna.5258504","relation":{},"ISSN":["1355-8382","1469-9001"],"issn-type":[{"value":"1355-8382","type":"print"},{"value":"1469-9001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,3,22]]}}}