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Wakayama T, Yanagimachi R. Development of normal mice from oocytes injected with freeze-dried spermatozoa. <i>Nat Biotechnol<\/i> 1998; 16: 639\u2013641.","DOI":"10.1038\/nbt0798-639"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2.  Hirabayashi M, Kato M, Ito J, Hochi S. Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads. <i>Zygote<\/i> 2005; 13: 79\u201385.","DOI":"10.1017\/S096719940500300X"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3.  Hochi S, Watanabe K, Kato M, Hirabayashi M. Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year. <i>Mol Reprod Dev<\/i> 2008; 75: 890\u2013894.","DOI":"10.1002\/mrd.20825"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4.  Muneto N, Horiuchi T. Full-term development of hamster embryos produced by injecting freeze-dried spermatozoa into oocytes. <i>J Mamm Ova Res<\/i> 2011; 28: 32\u201339.","DOI":"10.1274\/jmor.28.32"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5.  Liu JL, Kusakabe H, Chang CC, Suzuki H, Schmidt DW, Julian M, Pfeffer R, Bormann CL, Tian XC, Yanagimachi R, Yang X. Freeze-dried sperm fertilization leads to full-term development in rabbits. <i>Biol Reprod<\/i> 2004; 70: 1776\u20131781.","DOI":"10.1095\/biolreprod.103.025957"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6.  Choi YH, Varner DD, Love CC, Hartman DL, Hinrichs K. Production of live foals via intracytoplasmic injection of lyophilized sperm and sperm extract in the horse. <i>Reproduction<\/i> 2011; 142: 529\u2013538.","DOI":"10.1530\/REP-11-0145"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7.  Kusakabe H, Szczygiel MA, Whittingham DG, Yanagimachi R. Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa. <i>Proc Natl Acad Sci USA<\/i> 2001; 98: 13501\u201313506.","DOI":"10.1073\/pnas.241517598"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8.  Janny L, Menezo YJ. Evidence for a strong paternal effect on human preimplantation embryo development and blastocyst formation. <i>Mol Reprod Dev<\/i> 1994; 38: 36\u201342.","DOI":"10.1002\/mrd.1080380107"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9.  Men NT, Kikuchi K, Nakai M, Fukuda A, Tanihara F, Noguchi J, Kaneko H, Linh NV, Nguyen BX, Nagai T, Tajima A. Effect of trehalose on DNA integrity of freeze-dried boar sperm, fertilization, and embryo development after intracytoplasmic sperm injection. <i>Theriogenology<\/i> 2013; 80: 1033\u20131044.","DOI":"10.1016\/j.theriogenology.2013.08.001"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10.  Olaciregui M, Lu\u00f1o V, Gonz\u00e1lez N, Domingo P, de Blas I, Gil L. Chelating agents in combination with rosmarinic acid for boar sperm freeze-drying. <i>Reprod Biol<\/i> 2017; 17: 193\u2013198.","DOI":"10.1016\/j.repbio.2017.05.001"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11.  Kawase Y, Wada NA, Jishage K. Evaluation of DNA fragmentation of freeze-dried mouse sperm using a modified sperm chromatin structure assay. <i>Theriogenology<\/i> 2009; 72: 1047\u20131053.","DOI":"10.1016\/j.theriogenology.2009.06.021"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12.  Enciso M, Sarasa J, Agarwal A, Fern\u00e1ndez JL, Gos\u00e1lvez J. A two-tailed Comet assay for assessing DNA damage in spermatozoa. <i>Reprod Biomed Online<\/i> 2009; 18: 609\u2013616.","DOI":"10.1016\/S1472-6483(10)60003-X"},{"key":"13","unstructured":"13.  Cort\u00e9s-Guti\u00e9rrez EI, L\u00f3pez-Fern\u00e1ndez C, Fern\u00e1ndez JL, D\u00e1vila-Rodr\u00edguez MI, Johnston SD, Gos\u00e1lvez J. Interpreting sperm DNA damage in a diverse range of mammalian sperm by means of the two-tailed comet assay. <i>Front Genet<\/i> 2014; 5: 404."},{"key":"14","doi-asserted-by":"crossref","unstructured":"14.  Salamon S, Maxwell WMC. Storage of ram semen. <i>Anim Reprod Sci<\/i> 2000; 62: 77\u2013111.","DOI":"10.1016\/S0378-4320(00)00155-X"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15.  Anzalone DA, Palazzese L, Iuso D, Martino G, Loi P. Freeze-dried spermatozoa: An alternative biobanking option for endangered species. <i>Anim Reprod Sci<\/i> 2018; 190: 85\u201393.","DOI":"10.1016\/j.anireprosci.2018.01.010"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16.  Saragusty J, Hildebrandt TB, Behr B, Knieriem A, Kruse J, Hermes R. Successful cryopreservation of Asian elephant (Elephas maximus) spermatozoa. <i>Anim Reprod Sci<\/i> 2009; 115: 255\u2013266.","DOI":"10.1016\/j.anireprosci.2008.11.010"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17.  Saragusty J. Directional freezing for large volume cryopreservation. <i>In<\/i>: Wolkers WF, Oldenhof H (eds.), Methods in Cryopreservation and Freeze-Drying. New York: Springer Verlarg; 2015: 381\u2212397.","DOI":"10.1007\/978-1-4939-2193-5_19"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18.  Saragusty J, Walzer C, Petit T, Stalder G, Horowitz I, Hermes R. Cooling and freezing of epididymal sperm in the common hippopotamus (<i>Hippopotamus amphibius<\/i>). <i>Theriogenology<\/i> 2010; 74: 1256\u20131263.","DOI":"10.1016\/j.theriogenology.2010.05.031"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19.  Anzalone DA, Iuso D, Czernik M, Ptak G, Loi P. Plasma membrane and acrosome loss before ICSI is required for sheep embryonic development. <i>J Assist Reprod Genet<\/i> 2016; 33: 757\u2013763.","DOI":"10.1007\/s10815-016-0709-1"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20.  Loi P, Ledda S, Fulka J Jr, Cappai P, Moor RM. 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Freeze-dried stallion spermatozoa: evaluation of two chelating agents and comparative analysis of three sperm DNA damage assays. <i>Andrologia<\/i> 2016; 48: 900\u2013906.","DOI":"10.1111\/and.12530"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26.  Oldenhof H, Zhang M, Narten K, Bigalk J, Sydykov B, Wolkers WF, Sieme H. Freezing-induced uptake of disaccharides for preservation of chromatin in freeze-dried stallion sperm during accelerated aging. <i>Biol Reprod<\/i> 2017; 97: 892\u2013901.","DOI":"10.1093\/biolre\/iox142"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27.  Arav A, Saragusty J. Directional freezing of sperm and associated derived technologies. <i>Anim Reprod Sci<\/i> 2016; 169: 6\u201313.","DOI":"10.1016\/j.anireprosci.2016.02.007"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28.  Mio Y, Maeda K. 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