{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T04:58:51Z","timestamp":1785560331036,"version":"3.56.0"},"reference-count":32,"publisher":"Japanese Society of Animal Reproduction","issue":"4","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82271643"],"award-info":[{"award-number":["82271643"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"crossref","award":["ZR2024MH207"],"award-info":[{"award-number":["ZR2024MH207"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Jinan Science and Technology Development Plan","award":["202134060"],"award-info":[{"award-number":["202134060"]}]},{"name":"Jinan Science and Technology Development Plan","award":["202225032"],"award-info":[{"award-number":["202225032"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Reprod. Dev.","Journal of Reproduction and Development"],"published-print":{"date-parts":[[2026]]},"DOI":"10.1262\/jrd.2025-064","type":"journal-article","created":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T22:08:39Z","timestamp":1772748519000},"page":"632-640","source":"Crossref","is-referenced-by-count":1,"title":["N-myristoyltransferase 1, a key gene for protein N-myristoylation, is dispensable for fertility in male mice"],"prefix":"10.1262","volume":"72","author":[{"given":"Yang","family":"LIU","sequence":"first","affiliation":[{"name":"Research Center of Basic Medicine, Jinan Central Hospital, Shandong University, Jinan 250013, China"},{"name":"Department of Clinical Laboratory, Xuanwu Hospital, National Clinical Research Center for Geriatric Diseases, Capital Medical University, Beijing 100053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvxia","family":"ZHANG","sequence":"additional","affiliation":[{"name":"The First People\u2019s Hospital of Kunshan, Suzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailing","family":"DING","sequence":"additional","affiliation":[{"name":"College of Medicine, Qilu University of Technology, Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"LI","sequence":"additional","affiliation":[{"name":"Medical Research and Laboratory Diagnostic Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"GAO","sequence":"additional","affiliation":[{"name":"Medical Research and Laboratory Diagnostic Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongzhuang","family":"WEN","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunshan","family":"WANG","sequence":"additional","affiliation":[{"name":"Research Center of Basic Medicine, Jinan Central Hospital, Shandong University, Jinan 250013, China"},{"name":"Basic Medical Research Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"WU","sequence":"additional","affiliation":[{"name":"Research Center of Basic Medicine, Jinan Central Hospital, Shandong University, Jinan 250013, China"},{"name":"Department of Reproductive Medicine, Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"349","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1. Krausz C, Riera-Escamilla A. Genetics of male infertility. Nat Rev Urol<\/i> 2018; 15: 369\u2013384.","DOI":"10.1038\/s41585-018-0003-3"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Neto FT, Bach PV, Najari BB, Li PS, Goldstein M. Spermatogenesis in humans and its affecting factors. Semin Cell Dev Biol<\/i> 2016; 59: 10\u201326.","DOI":"10.1016\/j.semcdb.2016.04.009"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. Rahman MS, Lee JS, Kwon WS, Pang MG. Sperm proteomics: road to male fertility and contraception. Int J Endocrinol<\/i> 2013; 2013: 360986.","DOI":"10.1155\/2013\/360986"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Jan SZ, Hamer G, Repping S, de Rooij DG, van Pelt AM, Vormer TL. Molecular control of rodent spermatogenesis. Biochim Biophys Acta<\/i> 2012; 1822: 1838\u20131850.","DOI":"10.1016\/j.bbadis.2012.02.008"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk MM. Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice. Proc Natl Acad Sci USA<\/i> 2016; 113: 7704\u20137710.","DOI":"10.1073\/pnas.1608458113"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Wang H, Xu X, Wang J, Qiao Y. The role of N-myristoyltransferase 1 in tumour development . Ann Med<\/i> 2023; 55: 1422\u20131430.","DOI":"10.1080\/07853890.2023.2193425"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Farazi TA, Waksman G, Gordon JI. The biology and enzymology of protein N-myristoylation. J Biol Chem<\/i> 2001; 276: 39501\u201339504.","DOI":"10.1074\/jbc.R100042200"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Meinnel T, Dian C, Giglione C. Myristoylation, an ancient protein modification mirroring eukaryogenesis and evolution. Trends Biochem Sci<\/i> 2020; 45: 619\u2013632.","DOI":"10.1016\/j.tibs.2020.03.007"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Dian C, P\u00e9rez-Dorado I, Rivi\u00e8re F, Asensio T, Legrand P, Ritzefeld M, Shen M, Cota E, Meinnel T, Tate EW, Giglione C. High-resolution snapshots of human N-myristoyltransferase in action illuminate a mechanism promoting N-terminal Lys and Gly myristoylation. Nat Commun<\/i> 2020; 11: 1132.","DOI":"10.1038\/s41467-020-14847-3"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Castrec B, Dian C, Ciccone S, Ebert CL, Bienvenut WV, Le Caer JP, Steyaert JM, Giglione C, Meinnel T. Structural and genomic decoding of human and plant myristoylomes reveals a definitive recognition pattern. Nat Chem Biol<\/i> 2018; 14: 671\u2013679.","DOI":"10.1038\/s41589-018-0077-5"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Tate EW, Soday L, de la Lastra AL, Wang M, Lin H. Protein lipidation in cancer: mechanisms, dysregulation and emerging drug targets . Nat Rev Cancer<\/i> 2024; 24: 240\u2013260.","DOI":"10.1038\/s41568-024-00666-x"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Yang SH, Shrivastav A, Kosinski C, Sharma RK, Chen MH, Berthiaume LG, Peters LL, Chuang PT, Young SG, Bergo MO. N-myristoyltransferase 1 is essential in early mouse development. J Biol Chem<\/i> 2005; 280: 18990\u201318995.","DOI":"10.1074\/jbc.M412917200"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Udenwobele DI, Su RC, Good SV, Ball TB, Varma Shrivastav S, Shrivastav A. Myristoylation: an important protein modification in the immune response. Front Immunol<\/i> 2017; 8: 751.","DOI":"10.3389\/fimmu.2017.00751"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Shrivastav A, Suri SS, Mohr R, Janardhan KS, Sharma RK, Singh B. Expression and activity of N-myristoyltransferase in lung inflammation of cattle and its role in neutrophil apoptosis. Vet Res<\/i> 2010; 41: 9.","DOI":"10.1051\/vetres\/2009057"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Thinon E, Morales-Sanfrutos J, Mann DJ, Tate EW. N-Myristoyltransferase inhibition induces er-stress, cell cycle arrest, and apoptosis in cancer cells. ACS Chem Biol<\/i> 2016; 11: 2165\u20132176.","DOI":"10.1021\/acschembio.6b00371"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. Ducker CE, Upson JJ, French KJ, Smith CD. Two N-myristoyltransferase isozymes play unique roles in protein myristoylation, proliferation, and apoptosis. Mol Cancer Res<\/i> 2005; 3: 463\u2013476.","DOI":"10.1158\/1541-7786.MCR-05-0037"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. Raju RV, Moyana TN, Sharma RK. N-Myristoyltransferase overexpression in human colorectal adenocarcinomas. Exp Cell Res<\/i> 1997; 235: 145\u2013154.","DOI":"10.1006\/excr.1997.3679"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18. Rajala RV, Radhi JM, Kakkar R, Datla RS, Sharma RK. Increased expression of N-myristoyltransferase in gallbladder carcinomas. Cancer<\/i> 2000; 88: 1992\u20131999.","DOI":"10.1002\/(SICI)1097-0142(20000501)88:9<1992::AID-CNCR3>3.3.CO;2-7"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19. Lu Y, Selvakumar P, Ali K, Shrivastav A, Bajaj G, Resch L, Griebel R, Fourney D, Meguro K, Sharma RK. Expression of N-myristoyltransferase in human brain tumors. Neurochem Res<\/i> 2005; 30: 9\u201313.","DOI":"10.1007\/s11064-004-9680-9"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20. Ma X, Yuan Y, Lu J, Li M, Yu Y, Liu J, Zhou J. Long noncoding RNA ANCR promotes migration, invasion, EMT progress and stemness of nasopharyngeal carcinoma cells via the miR-4731-5p\/NMT1 axis. Pathol Res Pract<\/i> 2021; 224: 153540.","DOI":"10.1016\/j.prp.2021.153540"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21. Maurer-Stroh S, Eisenhaber F. Myristoylation of viral and bacterial proteins. Trends Microbiol<\/i> 2004; 12: 178\u2013185.","DOI":"10.1016\/j.tim.2004.02.006"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22. Shrivastav A, Varma S, Lawman Z, Yang SH, Ritchie SA, Bonham K, Singh SM, Saxena A, Sharma RK. Requirement of N-myristoyltransferase 1 in the development of monocytic lineage. J Immunol<\/i> 2008; 180: 1019\u20131028.","DOI":"10.4049\/jimmunol.180.2.1019"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23. Tan XP, He Y, Yang J, Wei X, Fan YL, Zhang GG, Zhu YD, Li ZQ, Liao HX, Qin DJ, Guan XY, Li B. Blockade of NMT1 enzymatic activity inhibits N-myristoylation of VILIP3 protein and suppresses liver cancer progression. Signal Transduct Target Ther<\/i> 2023; 8: 14.","DOI":"10.1038\/s41392-022-01248-9"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24. Mousnier A, Bell AS, Swieboda DP, Morales-Sanfrutos J, P\u00e9rez-Dorado I, Brannigan JA, Newman J, Ritzefeld M, Hutton JA, Gued\u00e1n A, Asfor AS, Robinson SW, Hopkins-Navratilova I, Wilkinson AJ, Johnston SL, Leatherbarrow RJ, Tuthill TJ, Solari R, Tate EW. Fragment-derived inhibitors of human N-myristoyltransferase block capsid assembly and replication of the common cold virus. Nat Chem<\/i> 2018; 10: 599\u2013606.","DOI":"10.1038\/s41557-018-0039-2"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25. Kim S, Alsaidan OA, Goodwin O, Li Q, Sulejmani E, Han Z, Bai A, Albers T, Beharry Z, Zheng YG, Norris JS, Szulc ZM, Bielawska A, Lebedyeva I, Pegan SD, Cai H. Blocking myristoylation of src inhibits its kinase activity and suppresses prostate cancer progression. Cancer Res<\/i> 2017; 77: 6950\u20136962.","DOI":"10.1158\/0008-5472.CAN-17-0981"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26. Wright MH, Clough B, Rackham MD, Rangachari K, Brannigan JA, Grainger M, Moss DK, Bottrill AR, Heal WP, Broncel M, Serwa RA, Brady D, Mann DJ, Leatherbarrow RJ, Tewari R, Wilkinson AJ, Holder AA, Tate EW. Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach. Nat Chem<\/i> 2014; 6: 112\u2013121.","DOI":"10.1038\/nchem.1830"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27. Wright MH, Heal WP, Mann DJ, Tate EW. Protein myristoylation in health and disease. J Chem Biol<\/i> 2010; 3: 19\u201335.","DOI":"10.1007\/s12154-009-0032-8"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28. Thinon E, Serwa RA, Broncel M, Brannigan JA, Brassat U, Wright MH, Heal WP, Wilkinson AJ, Mann DJ, Tate EW. Global profiling of co- and post-translationally N-myristoylated proteomes in human cells. Nat Commun<\/i> 2014; 5: 4919.","DOI":"10.1038\/ncomms5919"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29. Mou Z, Wang X, Fu Z, Dai Y, Han C, Ouyang J, Bao F, Hu Y, Li J. Silencing of phosphoethanolamine N-methyltransferase results in temperature-sensitive male sterility and salt hypersensitivity in Arabidopsis. Plant Cell<\/i> 2002; 14: 2031\u20132043.","DOI":"10.1105\/tpc.001701"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30. Dai SY, Hsu WH, Yang CH. The Gene ANTHER DEHISCENCE REPRESSOR (ADR) controls male fertility by suppressing the ros accumulation and anther cell wall thickening in arabidopsis. Sci Rep<\/i> 2019; 9: 5112.","DOI":"10.1038\/s41598-019-41382-z"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31. Pierre M, Traverso JA, Boisson B, Domenichini S, Bouchez D, Giglione C, Meinnel T. N-myristoylation regulates the SnRK1 pathway in Arabidopsis. Plant Cell<\/i> 2007; 19: 2804\u20132821.","DOI":"10.1105\/tpc.107.051870"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32. Rioux V, Beauchamp E, Pedrono F, Daval S, Molle D, Catheline D, Legrand P. Identification and characterization of recombinant and native rat myristoyl-CoA: protein N-myristoyltransferases. Mol Cell Biochem<\/i> 2006; 286: 161\u2013170.","DOI":"10.1007\/s11010-005-9108-0"}],"container-title":["Journal of Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/4\/72_2025-064\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T04:23:58Z","timestamp":1785558238000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/4\/72_2025-064\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.1262\/jrd.2025-064","relation":{},"ISSN":["0916-8818","1348-4400"],"issn-type":[{"value":"0916-8818","type":"print"},{"value":"1348-4400","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"2025-064"}}