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Vishwanath                     R.                  Artificial insemination: the state of the art.                     <i>Theriogenology<\/i>               2003; 59:                  571\u2013584.","DOI":"10.1016\/S0093-691X(02)01241-4"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2.  L\u00f3pez-Gatius                     F. Site                  of semen deposition in cattle: a review.                     <i>Theriogenology<\/i>               2000; 53:                  1407\u20131414.","DOI":"10.1016\/S0093-691X(00)00283-1"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3.  Brackett                     BG,                        Bousquet                     D,                        Boice                     ML,                        Donawick                     WJ,                        Evans                     JF,                        Dressel                     MA.                  Normal development following in vitro fertilization in the                  cow. <i>Biol Reprod<\/i>               1982; 27:                  147\u2013158.","DOI":"10.1095\/biolreprod27.1.147"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4.  Sheldon                     IM,                        Cronin                     JG,                        Healey                     GD,                        Gabler                     C,                        Heuwieser                     W,                        Streyl                     D,                        Bromfield                     JJ,                        Miyamoto                     A,                        Fergani                     C,                        Dobson                     H.                  Innate immunity and inflammation of the bovine female reproductive                  tract in health and disease. <i>Reproduction<\/i>               2014; 148:                  R41\u2013R51.","DOI":"10.1530\/REP-14-0163"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5.  Akthar                     I,                        Marey                     MA,                        Kim                     Y,                        Shimada                     M,                        Suarez                     SS,                        Miyamoto                     A.                  Sperm interaction with the uterine innate immune system: toll-like                  receptor 2 (TLR2) is a main sensor in cattle.                     <i>Reprod Fertil Dev<\/i>               2021; 34:                  139\u2013148.","DOI":"10.1071\/RD21265"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6.  Talukder                     AK,                        Yousef                     MS,                        Rashid                     MB,                        Awai                     K,                        Acosta                     TJ,                        Shimizu                     T,                        Okuda                     K,                        Shimada                     M,                        Imakawa                     K,                        Miyamoto                     A.                  Bovine embryo induces an anti-inflammatory response in uterine                  epithelial cells and immune cells in vitro: possible involvement of interferon tau                  as an intermediator. <i>J Reprod Dev<\/i>               2017; 63:                  425\u2013434.","DOI":"10.1262\/jrd.2017-056"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7.  Dobrowolski                     W,                        Hafez                     ES.                  Transport and distribution of spermatozoa in the reproductive tract                  of the cow. <i>J Anim Sci<\/i>               1970; 31:                  940\u2013943.","DOI":"10.2527\/jas1970.315940x"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8.  Hawk                     HW.                  Transport and fate of spermatozoa after insemination of                  cattle. <i>J Dairy Sci<\/i>               1987; 70:                  1487\u20131503.","DOI":"10.3168\/jds.S0022-0302(87)80173-X"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9.  Marey                     MA,                        Ma                     D,                        Yoshino                     H,                        Elesh                     IF,                        Zinnah                     MA,                        Fiorenza                     MF,                        Moriyasu                     S,                        Miyamoto                     A.                  Sperm induce proinflammatory responses in the uterus and peripheral                  blood immune cells of artificially inseminated cows.                     <i>J Reprod Dev<\/i>               2023; 69:               95\u2013102.","DOI":"10.1262\/jrd.2022-124"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10.  Elweza                     AE,                        Ezz                     MA,                        Acosta                     TJ,                        Talukder                     AK,                        Shimizu                     T,                        Hayakawa                     H,                        Shimada                     M,                        Imakawa                     K,                        Zaghloul                     AH,                        Miyamoto                     A. A                  proinflammatory response of bovine endometrial epithelial cells to active sperm in                  vitro. <i>Mol Reprod Dev<\/i>               2018; 85:                  215\u2013226.","DOI":"10.1002\/mrd.22955"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11.  Akthar                     I,                        Suarez                     SS,                        Morillo                     VA,                        Sasaki                     M,                        Ezz                     MA,                        Takahashi                     KI,                        Shimada                     M,                        Marey                     MA,                        Miyamoto                     A.                  Sperm enter glands of preovulatory bovine endometrial explants and                  initiate inflammation. <i>Reproduction<\/i>               2020; 159:                  181\u2013192.","DOI":"10.1530\/REP-19-0414"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12.  Janeway                     CA                     Jr, Medzhitov                     R.                  Innate immune recognition. <i>Annu Rev                     Immunol<\/i>               2002; 20:                  197\u2013216.","DOI":"10.1146\/annurev.immunol.20.083001.084359"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13.  Akira                     S,                        Takeda                     K,                        Kaisho                     T.                  Toll-like receptors: critical proteins linking innate and acquired                  immunity. <i>Nat Immunol<\/i>               2001; 2:               675\u2013680.","DOI":"10.1038\/90609"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14.  Schaefer                     TM,                        Desouza                     K,                        Fahey                     JV,                        Beagley                     KW,                        Wira                     CR.                  Toll-like receptor (TLR) expression and TLR-mediated                  cytokine\/chemokine production by human uterine epithelial cells.                     <i>Immunology<\/i>               2004; 112:                  428\u2013436.","DOI":"10.1111\/j.1365-2567.2004.01898.x"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15.  Gabler                     C,                        Fischer                     C,                        Drillich                     M,                        Einspanier                     R,                        Heuwieser                     W.                  Time-dependent mRNA expression of selected pro-inflammatory factors                  in the endometrium of primiparous cows postpartum.                     <i>Reprod Biol Endocrinol<\/i>               2010; 8: 152.","DOI":"10.1186\/1477-7827-8-152"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16.  Davies                     D,                        Meade                     KG,                        Herath                     S,                        Eckersall                     PD,                        Gonzalez                     D,                        White                     JO,                        Conlan                     RS,                        O\u2019Farrelly                     C,                        Sheldon                     IM.                  Toll-like receptor and antimicrobial peptide expression in the                  bovine endometrium. <i>Reprod Biol                     Endocrinol<\/i>               2008; 6: 53.","DOI":"10.1186\/1477-7827-6-53"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17.  Mogensen                     TH.                  Pathogen recognition and inflammatory signaling in innate immune                  defenses. <i>Clin Microbiol Rev<\/i>               2009; 22:                  240\u2013273.","DOI":"10.1128\/CMR.00046-08"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18.  Shimada                     M,                        Yanai                     Y,                        Okazaki                     T,                        Noma                     N,                        Kawashima                     I,                        Mori                     T,                        Richards                     JS.                  Hyaluronan fragments generated by sperm-secreted hyaluronidase                  stimulate cytokine\/chemokine production via the TLR2 and TLR4 pathway in cumulus                  cells of ovulated COCs, which may enhance fertilization.                     <i>Development<\/i>               2008; 135:                  2001\u20132011.","DOI":"10.1242\/dev.020461"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19.  Akthar                     I,                        Kim                     Y,                        Umehara                     T,                        Kanno                     C,                        Sasaki                     M,                        Marey                     MA,                        Yousef                     MS,                        Haneda                     S,                        Shimada                     M,                        Miyamoto                     A.                  Activation of sperm toll-like receptor 2 induces hyperactivation to                  enhance the penetration to mucus and uterine glands: a trigger for the uterine                  inflammatory cascade in cattle. <i>Front                     Immunol<\/i>               2023; 14: 1319572.","DOI":"10.3389\/fimmu.2023.1319572"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20.  Ma                     D,                        Marey                     MA,                        Shimada                     M,                        Miyamoto                     A.                  Toll-like receptor 2 is involved in calcium influx and acrosome                  reaction to facilitate sperm penetration to oocytes during in vitro fertilization                  in cattle. <i>Front Cell Dev Biol<\/i>               2022; 10: 810961.","DOI":"10.3389\/fcell.2022.810961"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21.  Gillan                     L,                        Evans                     G,                        Maxwell                     WM.                  Capacitation status and fertility of fresh and frozen-thawed ram                  spermatozoa. <i>Reprod Fertil Dev<\/i>               1997; 9:               481\u2013487.","DOI":"10.1071\/R96046"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22.  Bailey                     JL,                        Bilodeau                     JF,                        Cormier                     N.                  Semen cryopreservation in domestic animals: a damaging and                  capacitating phenomenon. <i>J Androl<\/i>               2000; 21: 1\u20137.","DOI":"10.1002\/j.1939-4640.2000.tb03268.x"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23.  Cormier                     N,                        Bailey                     JL. A                  differential mechanism is involved during heparin- and cryopreservation-induced                  capacitation of bovine spermatozoa. <i>Biol                     Reprod<\/i>               2003; 69:                  177\u2013185.","DOI":"10.1095\/biolreprod.102.011056"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24.  Elesh                     IF,                        Marey                     MA,                        Zinnah                     MA,                        Akthar                     I,                        Kawai                     T,                        Naim                     F,                        Goda                     W,                        Rawash                     ARA,                        Sasaki                     M,                        Shimada                     M,                        Miyamoto                     A.                  Peptidoglycan switches off the TLR2-mediated sperm recognition and                  triggers sperm localization in the bovine endometrium.                     <i>Front Immunol<\/i>               2021; 11: 619408.","DOI":"10.3389\/fimmu.2020.619408"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25.  Mansouri                     A,                        Yousef                     MS,                        Kowsar                     R,                        Usui                     N,                        Akthar                     I,                        Miyamoto                     A.                  Sperm activate TLR2\/TLR1 heterodimerization to induce a weak                  proinflammatory response in the bovine uterus.                     <i>Front Immunol<\/i>               2023; 14: 1158090.","DOI":"10.3389\/fimmu.2023.1158090"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26.  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Mansouri                     A,                        Akthar                     I,                        Miyamoto                     A. TLR2                  and TLR4 bridge physiological and pathological inflammation in the reproductive                  system. <i>Commun Biol<\/i>               2025; 8: 1008.","DOI":"10.1038\/s42003-025-08424-x"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28.  Monlish                     DA,                        Greenberg                     ZJ,                        Bhatt                     ST,                        Leonard                     KM,                        Romine                     MP,                        Dong                     Q,                        Bendesky                     L,                        Duncavage                     EJ,                        Magee                     JA,                        Schuettpelz                     LG.                  TLR2\/6 signaling promotes the expansion of premalignant                  hematopoietic stem and progenitor cells in the NUP98-HOXD13 mouse model of                  MDS. <i>Exp Hematol<\/i>               2020; 88: 42\u201355.","DOI":"10.1016\/j.exphem.2020.07.001"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29.  Erridge                     C.                  Endogenous ligands of TLR2 and TLR4: agonists or                  assistants? <i>J Leukoc Biol<\/i>               2010; 87:                  989\u2013999.","DOI":"10.1189\/jlb.1209775"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30.  Vuorio                     J,                        Vattulainen                     I,                        Martinez-Seara                     H.                  Atomistic fingerprint of hyaluronan-CD44 binding.                     <i>PLOS Comput Biol<\/i>               2017; 13: e1005663.","DOI":"10.1371\/journal.pcbi.1005663"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31.  Ezz                     MA,                        Mansouri                     A,                        Akthar                     I,                        Yousef                     MS,                        Kowsar                     R,                        Miyamoto                     A.                  Hyaluronan regulates sperm-induced inflammatory response by                  enhancing sperm attachment to bovine endometrial epithelial cells                     <i>via<\/i> CD44: <i>in-silico<\/i> and                     <i>in-vitro<\/i> approaches. <i>Front                     Endocrinol (Lausanne)<\/i>               2023; 14: 1134868.","DOI":"10.3389\/fendo.2023.1134868"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32.  Appiah                     MO,                        Wang                     J, Lu                     W.                  Microflora in the reproductive tract of cattle.                     <i>Rev Agric (Piracicaba)<\/i>               2020; 10: 232.","DOI":"10.3390\/agriculture10060232"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33.  Ballas                     P,                        Reinl\u00e4nder                     U,                        Schlegl                     R,                        Ehling-Schulz                     M,                        Drillich                     M,                        Wagener                     K.                  Characterization of intrauterine cultivable aerobic microbiota at                  the time of insemination in dairy cows with and without mild                  endometritis. <i>Theriogenology<\/i>               2021; 159:               28\u201334.","DOI":"10.1016\/j.theriogenology.2020.10.018"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34.  Vishwanath                     R,                        Shannon                     P.                  Storage of bovine semen in liquid and frozen state.                     <i>Anim Reprod Sci<\/i>               2000; 62: 23\u201353.","DOI":"10.1016\/S0378-4320(00)00153-6"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35.  Hulugalla                     M,                        Mansouri                     A,                        Waehama                     E,                        Akthar                     I,                        Miyamoto                     A.                  Semen extender triggers a mild physiological inflammatory response                  in the uterus without disrupting sperm-uterine immune crosstalk in vitro in                  cattle. <i>J Reprod Dev<\/i>               2025; 71: 24\u201334.","DOI":"10.1262\/jrd.2024-093"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36.  Akthar                     I,                        Hulugalla                     M,                        Sakai                     K,                        Waehama                     E,                        Mansouri                     A,                        Miyamoto                     A.                  Seminal plasma and semen extender: TLR2-mediated immune modulation                  in bovine endometrium in vitro. <i>J Reprod                     Dev<\/i>               2025; 71(Suppl):                  72.","DOI":"10.1262\/jrd.2024-093"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37.  Abeyrathne                     EDNS,                        Nam                     KC,                        Huang                     X,                        Ahn                     DU. Egg                  yolk lipids: separation, characterization, and utilization.                     <i>Food Sci Biotechnol<\/i>               2022; 31:                  1243\u20131256.","DOI":"10.1007\/s10068-022-01138-4"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38.  Kuss-Duerkop                     SK,                        Keestra-Gounder                     AM.                  NOD1 and NOD2 activation by diverse stimuli: A possible role for                  sensing pathogen-induced endoplasmic reticulum stress.                     <i>Infect Immun<\/i>               2020; 88:                  e00898\u2013e19.","DOI":"10.1128\/IAI.00898-19"},{"key":"39","doi-asserted-by":"crossref","unstructured":"39.  Almeida-da-Silva                     CLC,                        Savio                     LEB,                        Coutinho-Silva                     R,                        Ojcius                     DM. The                  role of NOD-like receptors in innate immunity.                     <i>Front Immunol<\/i>               2023; 14: 1122586.","DOI":"10.3389\/fimmu.2023.1122586"},{"key":"40","doi-asserted-by":"crossref","unstructured":"40.  Sheldon                     IM,                        Lewis                     GS,                        LeBlanc                     S,                        Gilbert                     RO.                  Defining postpartum uterine disease in cattle.                     <i>Theriogenology<\/i>               2006; 65:                  1516\u20131530.","DOI":"10.1016\/j.theriogenology.2005.08.021"},{"key":"41","doi-asserted-by":"crossref","unstructured":"41.  Oliveira-Nascimento                     L,                        Massari                     P,                        Wetzler                     LM. The                  Role of TLR2 in Infection and Immunity. <i>Front                     Immunol<\/i>               2012; 3: 79.","DOI":"10.3389\/fimmu.2012.00079"},{"key":"42","doi-asserted-by":"crossref","unstructured":"42.  Wu                     Z,                        Pan                     D,                        Guo                     Y,                        Sun                     Y,                        Zeng                     X.                  Peptidoglycan diversity and anti-inflammatory capacity in                  Lactobacillus strains. <i>Carbohydr Polym<\/i>               2015; 128:                  130\u2013137.","DOI":"10.1016\/j.carbpol.2015.04.026"},{"key":"43","doi-asserted-by":"crossref","unstructured":"43.  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Akthar                     I,                        Yousef                     MS,                        Mansouri                     A,                        Shimada                     M,                        Miyamoto                     A.                  Sperm hyperactivation in the uterus and oviduct: a double-edged                  sword for sperm and maternal innate immunity toward fertility.                     <i>Anim Reprod<\/i>               2024; 21: e20240043.","DOI":"10.1590\/1984-3143-ar2024-0043"},{"key":"46","doi-asserted-by":"crossref","unstructured":"46.  Brackett                     BG,                        Bousquet                     D,                        Boice                     ML,                        Donawick                     WJ,                        Evans                     JF,                        Dressel                     MA.                  Normal development following in vitro fertilization in the                  cow. <i>Biol Reprod<\/i>               1982; 27:                  147\u2013158.","DOI":"10.1095\/biolreprod27.1.147"},{"key":"47","doi-asserted-by":"crossref","unstructured":"47.  Ma                     D,                        Akthar                     I,                        Hashimoto                     T,                        Marey                     MA,                        Kusama                     K,                        Imakawa                     K,                        Shimada                     M,                        Miyamoto                     A.                  Toll-like receptor 2 activation of early divided bovine embryo                  promotes its viability and development competence in vitro.                     <i>Sci Rep<\/i>               2025; 15: 24678.","DOI":"10.1038\/s41598-025-09570-2"},{"key":"48","doi-asserted-by":"crossref","unstructured":"48.  Stachecki                     JJ,                        Yelian                     FD,                        Leach                     RE,                        Armant                     DR.                  Mouse blastocyst outgrowth and implantation rates following                  exposure to ethanol or A23187 during culture in vitro.                     <i>J Reprod Fertil<\/i>               1994; 101:                  611\u2013617.","DOI":"10.1530\/jrf.0.1010611"},{"key":"49","doi-asserted-by":"crossref","unstructured":"49.  Stachecki                     JJ,                        Yelian                     FD,                        Schultz                     JF,                        Leach                     RE,                        Armant                     DR.                  Blastocyst cavitation is accelerated by ethanol- or                  ionophore-induced elevation of intracellular calcium.                     <i>Biol Reprod<\/i>               1994; 50: 1\u20139.","DOI":"10.1095\/biolreprod50.1.1"},{"key":"50","doi-asserted-by":"crossref","unstructured":"50.  Bettegowda                     A,                        Patel                     OV,                        Lee                     KB,                        Park                     KE,                        Salem                     M,                        Yao                     J,                        Ireland                     JJ,                        Smith                     GW.                  Identification of novel bovine cumulus cell molecular markers                  predictive of oocyte competence: functional and diagnostic                  implications. <i>Biol Reprod<\/i>               2008; 79:                  301\u2013309.","DOI":"10.1095\/biolreprod.107.067223"},{"key":"51","doi-asserted-by":"crossref","unstructured":"51.  Balboula                     AZ,                        Yamanaka                     K,                        Sakatani                     M,                        Hegab                     AO,                        Zaabel                     SM,                        Takahashi                     M.                  Intracellular cathepsin B activity is inversely correlated with the                  quality and developmental competence of bovine preimplantation                  embryos. <i>Mol Reprod Dev<\/i>               2010; 77:                  1031\u20131039.","DOI":"10.1002\/mrd.21250"},{"key":"52","doi-asserted-by":"crossref","unstructured":"52.  Shen                     X,                        Zhang                     N,                        Wang                     Z,                        Bai                     G,                        Zheng                     Z, Gu                     Y, Wu                     Y,                        Liu                     H,                        Zhou                     D,                        Lei                     L.                  Induction of autophagy improves embryo viability in cloned mouse                  embryos. <i>Sci Rep<\/i>               2015; 5: 17829.","DOI":"10.1038\/srep17829"}],"container-title":["Journal of Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/3\/72_2025-124\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T03:24:23Z","timestamp":1781925863000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/3\/72_2025-124\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":52,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.1262\/jrd.2025-124","relation":{},"ISSN":["0916-8818","1348-4400"],"issn-type":[{"value":"0916-8818","type":"print"},{"value":"1348-4400","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"2025-124"}}