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Effective vitrification and warming of porcine embryos using a pH-stable, chemically defined medium. Sci Rep<\/i> 2016; 6: 33915.","DOI":"10.1038\/srep33915"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Martinez CA, Cambra JM, Nohalez A, Parrilla I, Roca J, Vazquez JL, Rodriguez-Martinez H, Gil MA, Martinez EA, Cuello C. Prevention of hatching of porcine morulae and blastocysts by liquid storage at 20 \u00b0C. Sci Rep<\/i> 2019; 9: 6219.","DOI":"10.1038\/s41598-019-42712-x"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. Gonzalez-Plaza A, Cambra JM, Garcia-Canovas M, Parrilla I, Gil MA, Martinez EA, Rodriguez-Martinez H, Martinez CA, Cuello C. Cryotop vitrification of large batches of pig embryos simultaneously provides excellent postwarming survival rates and minimal interference with gene expression. Theriogenology<\/i> 2023; 206: 1\u201310.","DOI":"10.1016\/j.theriogenology.2023.04.011"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Gil MA, Parrilla I, Cuello C, Martinez EA. Current status of nonsurgical embryo transfer in swine. Reprod Fertil Dev<\/i> 2024; 37.","DOI":"10.1071\/RD24137"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Martinez EA, Martinez CA, Cambra JM, Maside C, Lucas X, Vazquez JL, Vazquez JM, Roca J, Rodriguez-Martinez H, Gil MA, Parrilla I, Cuello C. Achievements and future perspectives of embryo transfer technology in pigs. Reprod Domest Anim<\/i> 2019; 54(Suppl 4): 4\u201313.","DOI":"10.1111\/rda.13465"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Martinez-Serrano CA, Martinez EA. The swine revolution: Can embryo transfer transform the industry? Livest Sci<\/i> 2025; 300: 105793.","DOI":"10.1016\/j.livsci.2025.105793"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Br\u00fcssow KP, Torner H, Kanitz W, R\u00e1tky J. In vitro technologies related to pig embryo transfer. 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Animal Model Exp Med<\/i> 2022; 5: 141\u2013152.","DOI":"10.1002\/ame2.12223"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Perleberg C, Kind A, Schnieke A. Genetically engineered pigs as models for human disease. Dis Model Mech<\/i> 2018; 11: dmm030783.","DOI":"10.1242\/dmm.030783"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Walters EM, Wells KD, Bryda EC, Schommer S, Prather RS. Swine models, genomic tools and services to enhance our understanding of human health and diseases. Lab Anim (NY)<\/i> 2017; 46: 167\u2013172.","DOI":"10.1038\/laban.1215"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Lei T, Chen L, Wang K, Du S, Gonelle-Gispert C, Wang Y, Buhler LH. Genetic engineering of pigs for xenotransplantation to overcome immune rejection and physiological incompatibilities: The first clinical steps. Front Immunol<\/i> 2022; 13: 1031185.","DOI":"10.3389\/fimmu.2022.1031185"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Briski O, La Motta GE, Ratner LD, Allegroni FA, Pillado S, \u00c1lvarez G, Gutierrez B, Tarragona L, Zaccagnini A, Acerbo M, Ciampi C, Fern\u00e1ndez-Martin R, Salamone DF. Comparison of ICSI, IVF, and in vivo derived embryos to produce CRISPR-Cas9 gene-edited pigs for xenotransplantation. Theriogenology<\/i> 2024; 220: 43\u201355.","DOI":"10.1016\/j.theriogenology.2024.02.028"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. Garcia-Canovas M, Parrilla I, Cuello C, Gil MA, Martinez EA. Swine in vitro embryo production: Potential, challenges, and advances. Anim Reprod Sci<\/i> 2024; 270: 107600.","DOI":"10.1016\/j.anireprosci.2024.107600"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. Fowler KE, Mandawala AA, Griffin DK, Walling GA, Harvey SC. The production of pig preimplantation embryos in vitro: Current progress and future prospects. 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Angel MA, Gil MA, Cuello C, Sanchez-Osorio J, Gomis J, Parrilla I, Vila J, Colina I, Diaz M, Reixach J, Vazquez JL, Vazquez JM, Roca J, Martinez EA. The effects of superovulation of donor sows on ovarian response and embryo development after nonsurgical deep-uterine embryo transfer. Theriogenology<\/i> 2014; 81: 832\u2013839.","DOI":"10.1016\/j.theriogenology.2013.12.017"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22. Hazeleger W, Bouwman EG, Noordhuizen JPTM, Kemp B. Effect of superovulation induction on embryonic development on day 5 and subsequent development and survival after nonsurgical embryo transfer in pigs. Theriogenology<\/i> 2000; 53: 1063\u20131070.","DOI":"10.1016\/S0093-691X(00)00252-1"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23. Patterson J, Wellen A, Hahn M, Pasternak A, Lowe J, DeHaas S, Kraus D, Williams N, Foxcroft G. Responses to delayed estrus after weaning in sows using oral progestagen treatment. 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Gonzalez-Ramiro H, Cuello C, Cambra JM, Gonzalez-Plaza A, Vazquez JM, Vazquez JL, Rodriguez-Martinez H, Gil MA, Lucas-Sanchez A, Parrilla I, Martinez EA. A short-term altrenogest treatment post-weaning followed by superovulation reduces pregnancy rates and embryo production efficiency in multiparous sows. Front Vet Sci<\/i> 2021; 8: 771573.","DOI":"10.3389\/fvets.2021.771573"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27. Gonzalez-Ramiro H, Gil MA, Cuello C, Cambra JM, Gonzalez-Plaza A, Vazquez JM, Vazquez JL, Rodriguez-Martinez H, Lucas-Sanchez A, Parrilla I, Martinez CA, Martinez EA. The use of a brief synchronization treatment after weaning, combined with superovulation, has moderate effects on the gene expression of surviving pig blastocysts. Animals (Basel)<\/i> 2023; 13: 1568.","DOI":"10.3390\/ani13091568"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28. Gonzalez-Ramiro H, Gil MA, Cuello C, Cambra JM, Gonzalez-Plaza A, Vazquez JM, Vazquez JL, Rodriguez-Martinez H, Lucas-Sanchez A, Parrilla I, Martinez CA, Martinez EA. The use of a brief synchronization treatment after weaning, combined with superovulation, has moderate effects on the gene expression of surviving pig blastocysts. Animals (Basel)<\/i> 2023; 13: 1568.","DOI":"10.3390\/ani13091568"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29. Martinat-Bott\u00e9 F, Bariteau F, Forgerit Y, Macar C, Poirier P, Terqui M. Synchronization of oestrus in gilts with altrenogest: effects on ovulation rate and foetal survival. Anim Reprod Sci<\/i> 1995; 39: 267\u2013274.","DOI":"10.1016\/0378-4320(95)01396-H"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30. Wang Z, Liu BS, Wang XY, Wei QH, Tian H, Wang LQ. Effects of altrenogest on reproductive performance of gilts and sows: A meta-analysis. 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Cuello C, Gonz\u00e1lez-Plaza A, Cambra JM, Garcia-Canovas M, Parrilla I, Rodriguez-Martinez H, Gil MA, Martinez EA. Vitrification of pig embryos dysregulates the microRNA transcriptome profile. Theriogenology<\/i> 2024; 226: 243\u2013252.","DOI":"10.1016\/j.theriogenology.2024.06.001"},{"key":"41","doi-asserted-by":"crossref","unstructured":"41. Cuello C, Martinez CA, Cambra JM, Parrilla I, Rodriguez-Martinez H, Gil MA, Martinez EA. Effects of vitrification on the blastocyst gene expression profile in a porcine model. Int J Mol Sci<\/i> 2021; 22: 1\u201314.","DOI":"10.3390\/ijms22031222"},{"key":"42","doi-asserted-by":"crossref","unstructured":"42. Gonzalez-Plaza A, Cambra JM, Parrilla I, Gil MA, Martinez EA, Martinez CA, Cuello C. The open cryotop system is effective for the simultaneous vitrification of a large number of porcine embryos at different developmental stages. Front Vet Sci<\/i> 2022; 9: 936753.","DOI":"10.3389\/fvets.2022.936753"},{"key":"43","doi-asserted-by":"crossref","unstructured":"43. Martinez CA, Nohalez A, Parrilla I, Vazquez JL, Roca J, Cuello C, Rodriguez-Martinez H, Martinez EA, Gil MA. Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows. Theriogenology<\/i> 2017; 87: 316\u2013320.","DOI":"10.1016\/j.theriogenology.2016.09.009"},{"key":"44","doi-asserted-by":"publisher","unstructured":"44. James JE, Reeser PD. Embryo recovery in swine. Theriogenology<\/i> 1979; 11: 47\u201350. https:\/\/doi.org\/https:\/\/doi.org\/10.1016\/S0093-691X(79)80017-5.","DOI":"10.1016\/S0093-691X(79)80017-5"},{"key":"45","doi-asserted-by":"publisher","unstructured":"45. Besenfelder U, M\u00f6dl J, M\u00fcller M, Brem G. Endoscopic embryo collection and embryo transfer into the oviduct and the uterus of pigs. 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Vitrification effects on the transcriptome of in vivo<\/i>-derived porcine morulae. Front Vet Sci<\/i> 2021; 8: 771996.","DOI":"10.3389\/fvets.2021.771996"},{"key":"56","doi-asserted-by":"crossref","unstructured":"56. Funahashi H, Ekwall H, Rodriguez-Martinez H. Zona reaction in porcine oocytes fertilized in vivo and in vitro as seen with scanning electron microscopy. Biol Reprod<\/i> 2000; 63: 1437\u20131442.","DOI":"10.1095\/biolreprod63.5.1437"},{"key":"57","doi-asserted-by":"crossref","unstructured":"57. Cuello C, Gonzalez-Plaza A, Parrilla I, Gil MA, Martinez EA. Vitrification: A reliable method for cryopreservation of animal embryos. In: Zsolt PN, Varghese AC, Agarwal A, editors. 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Whitworth KM, Lee K, Benne JA, Beaton BP, Spate LD, Murphy SL, Samuel MS, Mao J, O\u2019Gorman C, Walters EM, Murphy CN, Driver J, Mileham A, McLaren D, Wells KD, Prather RS. Use of the CRISPR\/Cas9 system to produce genetically engineered pigs from in vitro-derived oocytes and embryos. 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