{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T04:45:38Z","timestamp":1776487538533,"version":"3.51.2"},"reference-count":39,"publisher":"Japanese Society of Animal Reproduction","issue":"2","funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K08805"],"award-info":[{"award-number":["23K08805"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Reprod. Dev.","Journal of Reproduction and Development"],"published-print":{"date-parts":[[2026]]},"DOI":"10.1262\/jrd.2025-140","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:08:10Z","timestamp":1771625290000},"page":"73-79","source":"Crossref","is-referenced-by-count":0,"title":["Niacin improves developmental ability by increasing glutathione levels and promoting male pronuclear formation in porcine oocytes grown &lt;i&gt;in vitro&lt;\/i&gt;"],"prefix":"10.1262","volume":"72","author":[{"given":"Takayuki","family":"YAMOCHI","sequence":"first","affiliation":[{"name":"Reproductive Science, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, Japan"},{"name":"HORAC Grand Front Osaka Clinic, Osaka 530-0011, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu","family":"HASHIMOTO","sequence":"additional","affiliation":[{"name":"Reproductive Science, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiharu","family":"MORIMOTO","sequence":"additional","affiliation":[{"name":"HORAC Grand Front Osaka Clinic, Osaka 530-0011, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"349","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1.  Xiao S, Zhang J, Romero MM, Smith KN, Shea LD, Woodruff TK. <i>In vitro<\/i> follicle growth supports human oocyte meiotic maturation. <i>Sci Rep<\/i> 2015; 5: 17323.","DOI":"10.1038\/srep17323"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2.  Malo C, Oliv\u00e1n S, Ochoa I, Shikanov A. <i>In vitro<\/i> growth of human follicles: current and future perspectives. <i>Int J Mol Sci<\/i> 2024; 25: 1510.","DOI":"10.3390\/ijms25031510"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3.  Smitz J, Dolmans MM, Donnez J, Fortune JE, Hovatta O, Jewgenow K, Picton HM, Plancha C, Shea LD, Stouffer RL, Telfer EE, Woodruff TK, Zelinski MB. Current achievements and future research directions in ovarian tissue culture, <i>in vitro<\/i> follicle development and transplantation: implications for fertility preservation. <i>Hum Reprod Update<\/i> 2010; 16: 395\u2013414.","DOI":"10.1093\/humupd\/dmp056"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4.  Hirao Y. Conditions affecting growth and developmental competence of mammalian oocytes <i>in vitro<\/i>. <i>Anim Sci J<\/i> 2011; 82: 187\u2013197.","DOI":"10.1111\/j.1740-0929.2010.00870.x"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5.  Phuong LDT, Thien LC, Su Pham CD, Minh NU, Huy Bao NT, Thien Truc LN, Huyen TTN, Minh DT, Nguyen NT, Van Thuan N, Bui HT. Melatonin and cyclic adenosine monophosphate enhance the meiotic and developmental competence of porcine oocytes from early antral follicles during <i>in vitro<\/i> growth and pre-maturation culture. <i>Theriogenology<\/i> 2025; 237: 129\u2013142.","DOI":"10.1016\/j.theriogenology.2025.02.026"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6.  Valckx SD, Van Hoeck V, Arias-Alvarez M, Maillo V, Lopez-Cardona AP, Gutierrez-Adan A, Berth M, Cortvrindt R, Bols PE, Leroy JL. Elevated non-esterified fatty acid concentrations during <i>in vitro<\/i> murine follicle growth alter follicular physiology and reduce oocyte developmental competence. <i>Fertil Steril<\/i> 2014; 102: 1769\u201376.e1.","DOI":"10.1016\/j.fertnstert.2014.08.018"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7.  Shibahara H, Ishiguro A, Inoue Y, Koumei S, Kuwayama T, Iwata H. Mechanism of palmitic acid-induced deterioration of <i>in vitro<\/i> development of porcine oocytes and granulosa cells. <i>Theriogenology<\/i> 2020; 141: 54\u201361.","DOI":"10.1016\/j.theriogenology.2019.09.006"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8.  Kanaya H, Hashimoto S, Teramura T, Morimoto Y, Matsumoto K, Saeki K, Iritani A, Hosoi Y. Mitochondrial dysfunction of <i>in vitro<\/i> grown rabbit oocytes results in preimplantation embryo arrest after activation. <i>J Reprod Dev<\/i> 2007; 53: 631\u2013637.","DOI":"10.1262\/jrd.19010"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9.  Takashima T, Fujimaru T, Obata Y. Effect of in vitro growth on mouse oocyte competency, mitochondria and transcriptome. <i>Reproduction<\/i> 2021; 162: 307\u2013318.","DOI":"10.1530\/REP-21-0209"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10.  Van Blerkom J. Mitochondria in human oogenesis and preimplantation embryogenesis: engines of metabolism, ionic regulation and developmental competence. <i>Reproduction<\/i> 2004; 128: 269\u2013280.","DOI":"10.1530\/rep.1.00240"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11.  St John JC, Okada T, Andreas E, Penn A. The role of mtDNA in oocyte quality and embryo development. <i>Mol Reprod Dev<\/i> 2023; 90: 621\u2013633.","DOI":"10.1002\/mrd.23640"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12.  Reynier P, May-Panloup P, Chr\u00e9tien MF, Morgan CJ, Jean M, Savagner F, Barri\u00e8re P, Malthi\u00e8ry Y. Mitochondrial DNA content affects the fertilizability of human oocytes. <i>Mol Hum Reprod<\/i> 2001; 7: 425\u2013429.","DOI":"10.1093\/molehr\/7.5.425"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13.  Santos TA, El Shourbagy S, St John JC. Mitochondrial content reflects oocyte variability and fertilization outcome. <i>Fertil Steril<\/i> 2006; 85: 584\u2013591.","DOI":"10.1016\/j.fertnstert.2005.09.017"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14.  Mahrous E, Yang Q, Clarke HJ. Regulation of mitochondrial DNA accumulation during oocyte growth and meiotic maturation in the mouse. <i>Reproduction<\/i> 2012; 144: 177\u2013185.","DOI":"10.1530\/REP-12-0113"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15.  Cant\u00f3 C, Menzies KJ, Auwerx J. NAD(+) metabolism and the control of energy homeostasis: a balancing act between mitochondria and the nucleus. <i>Cell Metab<\/i> 2015; 22: 31\u201353.","DOI":"10.1016\/j.cmet.2015.05.023"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16.  Zhang T, Berrocal JG, Frizzell KM, Gamble MJ, DuMond ME, Krishnakumar R, Yang T, Sauve AA, Kraus WL. Enzymes in the NAD+ salvage pathway regulate SIRT1 activity at target gene promoters. <i>J Biol Chem<\/i> 2009; 284: 20408\u201320417.","DOI":"10.1074\/jbc.M109.016469"},{"key":"17","unstructured":"17.  Ganji S, Kamanna S, Kamanna VS, Kashyap ML. Niacin increases human aortic endothelial Sirt1 activity and nitric oxide: effect on endothelial function and vascular aging. <i>Am J Transl Res<\/i> 2023; 15: 6771\u20136778."},{"key":"18","doi-asserted-by":"crossref","unstructured":"18.  Ye L, Cao Z, Lai X, Shi Y, Zhou N. Niacin ameliorates hepatic steatosis by inhibiting de novo lipogenesis via a GPR109A-mediated PKC-ERK1\/2-AMPK signaling pathway in C57BL\/6 mice fed a high-fat diet. <i>J Nutr<\/i> 2020; 150: 672\u2013684.","DOI":"10.1093\/jn\/nxz303"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19.  Guo W, Liu J, Li W, Ma H, Gong Q, Kan X, Cao Y, Wang J, Fu S. Niacin alleviates dairy cow mastitis by regulating the GPR109A\/AMPK\/NRF2 signaling pathway. <i>Int J Mol Sci<\/i> 2020; 21: 3321.","DOI":"10.3390\/ijms21093321"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20.  Kov\u00e1cs Z, Brunner B, Ari C. Beneficial effects of exogenous ketogenic supplements on aging processes and age-related neurodegenerative diseases. <i>Nutrients<\/i> 2021; 13: 2197.","DOI":"10.3390\/nu13072197"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21.  Yamochi T, Hashimoto S, Morimoto Y. Mural granulosa cells support to maintain the viability of growing porcine oocytes and its developmental competence after insemination. <i>J Assist Reprod Genet<\/i> 2021; 38: 2591\u20132599.","DOI":"10.1007\/s10815-021-02212-2"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22.  Hashimoto S. Application of <i>in vitro<\/i> maturation to assisted reproductive technology. <i>J Reprod Dev<\/i> 2009; 55: 1\u201310.","DOI":"10.1262\/jrd.20127"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23.  Yoshioka K, Suzuki C, Onishi A. Defined system for <i>in vitro<\/i> production of porcine embryos using a single basic medium. <i>J Reprod Dev<\/i> 2008; 54: 208\u2013213.","DOI":"10.1262\/jrd.20001"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24.  Kageyama M, Ito J, Shirasuna K, Kuwayama T, Iwata H. Mitochondrial reactive oxygen species regulate mitochondrial biogenesis in porcine embryos. <i>J Reprod Dev<\/i> 2021; 67: 141\u2013147.","DOI":"10.1262\/jrd.2020-111"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25.  Abeydeera LR, Day BN. In vitro penetration of pig oocytes in a modified Tris-buffered medium: effect of BSA, caffeine and calcium. <i>Theriogenology<\/i> 1997; 48: 537\u2013544.","DOI":"10.1016\/S0093-691X(97)00270-7"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26.  Lapenna D. Glutathione and glutathione-dependent enzymes: From biochemistry to gerontology and successful aging. <i>Ageing Res Rev<\/i> 2023; 92: 102066.","DOI":"10.1016\/j.arr.2023.102066"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27.  Hardie DG. AMP-activated protein kinase: an energy sensor that regulates all aspects of cell function. <i>Genes Dev<\/i> 2011; 25: 1895\u20131908.","DOI":"10.1101\/gad.17420111"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28.  Chaube B, Malvi P, Singh SV, Mohammad N, Viollet B, Bhat MK. AMPK maintains energy homeostasis and survival in cancer cells via regulating p38\/PGC-1\u03b1-mediated mitochondrial biogenesis. <i>Cell Death Discov<\/i> 2015; 1: 15063.","DOI":"10.1038\/cddiscovery.2015.63"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29.  Choi HI, Kim HJ, Park JS, Kim IJ, Bae EH, Ma SK, Kim SW. PGC-1\u03b1 attenuates hydrogen peroxide-induced apoptotic cell death by upregulating Nrf-2 via GSK3\u03b2 inactivation mediated by activated p38 in HK-2 Cells. <i>Sci Rep<\/i> 2017; 7: 4319.","DOI":"10.1038\/s41598-017-04593-w"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30.  Khan M, Couturier A, Kubens JF, Most E, Mooren FC, Kr\u00fcger K, Ringseis R, Eder K. Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep. <i>Acta Vet Scand<\/i> 2013; 55: 85.","DOI":"10.1186\/1751-0147-55-85"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31.  Khan M, Ringseis R, Mooren FC, Kr\u00fcger K, Most E, Eder K. Niacin supplementation increases the number of oxidative type I fibers in skeletal muscle of growing pigs. <i>BMC Vet Res<\/i> 2013; 9: 177.","DOI":"10.1186\/1746-6148-9-177"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32.  Ringseis R, Rosenbaum S, Gessner DK, Herges L, Kubens JF, Mooren FC, Kr\u00fcger K, Eder K. Supplementing obese Zucker rats with niacin induces the transition of glycolytic to oxidative skeletal muscle fibers. <i>J Nutr<\/i> 2013; 143: 125\u2013131.","DOI":"10.3945\/jn.112.164038"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33.  Ringseis R, Gessner DK, Beer AM, Albrecht Y, Wen G, Most E, Kr\u00fcger K, Eder K. Nicotinic acid improves endurance performance of mice subjected to treadmill exercise. <i>Metabolites<\/i> 2020; 10: 138.","DOI":"10.3390\/metabo10040138"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34.  Perreault SD, Barbee RR, Slott VL. Importance of glutathione in the acquisition and maintenance of sperm nuclear decondensing activity in maturing hamster oocytes. <i>Dev Biol<\/i> 1988; 125: 181\u2013186.","DOI":"10.1016\/0012-1606(88)90070-X"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35.  Sutovsky P, Schatten G. Depletion of glutathione during bovine oocyte maturation reversibly blocks the decondensation of the male pronucleus and pronuclear apposition during fertilization. <i>Biol Reprod<\/i> 1997; 56: 1503\u20131512.","DOI":"10.1095\/biolreprod56.6.1503"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36.  Funahashi H, Cantley TC, Stumpf TT, Terlouw SL, Day BN. Use of low-salt culture medium for in vitro maturation of porcine oocytes is associated with elevated oocyte glutathione levels and enhanced male pronuclear formation after in vitro fertilization. <i>Biol Reprod<\/i> 1994; 51: 633\u2013639.","DOI":"10.1095\/biolreprod51.4.633"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37.  Maedomari N, Kikuchi K, Ozawa M, Noguchi J, Kaneko H, Ohnuma K, Nakai M, Shino M, Nagai T, Kashiwazaki N. Cytoplasmic glutathione regulated by cumulus cells during porcine oocyte maturation affects fertilization and embryonic development in vitro. <i>Theriogenology<\/i> 2007; 67: 983\u2013993.","DOI":"10.1016\/j.theriogenology.2006.11.012"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38.  Hu D, Zhang L, Jiang R, Liao C, Xu J, Jiang S, Yang Y, Lin L, Huang J, Shen Y, Tang L, Li L. Nicotinic acid against acetaminophen-induced hepatotoxicity via Sirt1\/Nrf2 antioxidative pathway in mice. <i>J Nutr Sci Vitaminol (Tokyo)<\/i> 2021; 67: 145\u2013152.","DOI":"10.3177\/jnsv.67.145"},{"key":"39","doi-asserted-by":"crossref","unstructured":"39.  Almubarak A, Osman R, Lee J, Yu IJ, Jeon Y. Effects of niacin supplementation during in vitro culture on the developmental competence of porcine embryos. <i>Reprod Domest Anim<\/i> 2023; 58: 1685\u20131694.","DOI":"10.1111\/rda.14483"}],"container-title":["Journal of Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/2\/72_2025-140\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T04:08:35Z","timestamp":1776485315000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/2\/72_2025-140\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.1262\/jrd.2025-140","relation":{},"ISSN":["0916-8818","1348-4400"],"issn-type":[{"value":"0916-8818","type":"print"},{"value":"1348-4400","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"2025-140"}}