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Theriogenology<\/i> 2016; 85: 83\u201393.","DOI":"10.1016\/j.theriogenology.2015.07.009"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Casas I, Flores E. Gene Banking: The freezing strategy. In: Bonet S, Casas I, Holt W V, Yeste M, editors. Boar Reproduction, Berlin, Heidelberg: Springer Berlin Heidelberg; 2013, p. 551\u201388.","DOI":"10.1007\/978-3-642-35049-8_11"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. Yeste M. State-of-the-art of boar sperm preservation in liquid and frozen state. Anim Reprod<\/i> 2017; 14.","DOI":"10.21451\/1984-3143-AR895"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Nerin C, Ubeda JL, Alfaro P, Dahmani Y, Aznar M, Canellas E, Ausejo R. Compounds from multilayer plastic bags cause reproductive failures in artificial insemination. Sci Rep<\/i> 2014; 4: 4913.","DOI":"10.1038\/srep04913"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Lacalle E, Mislei B, Algieri C, Spinaci M, Pastor FM, Nesci S, Morrell JM, Ortiz-Rodriguez JM, Bucci D. Mitochondrial bioenergetics analysis on SLC-selected boar spermatozoa during liquid storage. Anim Reprod Sci<\/i> 2025; 278: 107875.","DOI":"10.1016\/j.anireprosci.2025.107875"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Prieto OB, Algieri C, Spinaci M, Trombetti F, Nesci S, Bucci D. Cell bioenergetics and ATP production of boar spermatozoa. Theriogenology<\/i> 2023; 210: 162\u2013168.","DOI":"10.1016\/j.theriogenology.2023.07.018"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Medrano A, Garc\u00eda-Gil N, Rami\u00f3 L, Montserrat Rivera M, Fern\u00e1ndez-Novell JM, Ram\u00edrez A, Pe\u00f1a A, Dolors Briz M, Pinart E, Concha II, Bonet S, Rigau T, Rodr\u00edguez-Gil JE. Hexose-specificity of hexokinase and ADP-dependence of pyruvate kinase play important roles in the control of monosaccharide utilization in freshly diluted boar spermatozoa. Mol Reprod Dev<\/i> 2006; 73: 1179\u20131194.","DOI":"10.1002\/mrd.20480"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Johnson LA, Aalbers JG, Grooten HJG. Artificial insemination of swine: fecundity of boar semen stored in beltsville TS (BTS), modified modena (MM), or MR\u2010A and inseminated on one, three and four days after collection 1 2<\/sup>. Reprod Domest Anim<\/i> 1988; 23: 49\u201355.","DOI":"10.1111\/j.1439-0531.1988.tb01080.x"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Gadea J. Review: semen extenders used in the artificial inseminarion of swine. Span J Agric Res<\/i> 2003; 1: 17\u201327.","DOI":"10.5424\/sjar\/2003012-17"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Medrano A, Pe\u00f1a A, Rigau T, Rodr\u00ecguez-Gil JE. Variations in the proportion of glycolytic\/non-glycolytic energy substrates modulate sperm membrane integrity and function in diluted boar samples stored at 15-17 \u00b0 C. Reprod Domest Anim<\/i> 2005; 40: 448\u2013453.","DOI":"10.1111\/j.1439-0531.2005.00599.x"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Cheng J, Dong Q, Nie S, Hao X, Mo S, Liu Y, Zhu Z, Lu H, Zhang T, Zeng W. G6PD lactylation is involved in regulating redox balance of boar sperm in low glucose extender. Theriogenology<\/i> 2025; 239: 117388.","DOI":"10.1016\/j.theriogenology.2025.117388"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Zhang XG, Yan GJ, Hong JY, Su ZZ, Yang GS, Li QW, Hu JH. Effects of bovine serum albumin on boar sperm quality during liquid storage at 17\u00b0C. Reprod Domest Anim<\/i> 2015; 50: 263\u2013269.","DOI":"10.1111\/rda.12481"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. van den Berg BM, Reesink J, Reesink W. TRIXcell+, a new long-term boar semen extender containing whey protein with higher preservation capacity and litter size. Open Vet J<\/i> 2014; 4: 20\u201325.","DOI":"10.5455\/OVJ.2014.v4.i1.p20"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Park Y-J, Shin D-H, Pang W-K, Ryu D-Y, Rahman MS, Adegoke EO, Pang M-G. Short-term storage of semen samples in acidic extender increases the proportion of females in pigs. BMC Vet Res<\/i> 2021; 17: 362.","DOI":"10.1186\/s12917-021-03078-3"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Dub\u00e9 C, Beaulieu M, Reyes-Moreno C, Guillemette C, Bailey JL. Boar sperm storage capacity of BTS and Androhep Plus: viability, motility, capacitation, and tyrosine phosphorylation. Theriogenology<\/i> 2004; 62: 874\u2013886.","DOI":"10.1016\/j.theriogenology.2003.12.006"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. De Ambrogi M, Ballester J, Saravia F, Caballero I, Johannisson A, Wallgren M, Andersson M, Rodriguez-Martinez H. Effect of storage in short--and long-term commercial semen extenders on the motility, plasma membrane and chromatin integrity of boar spermatozoa. Int J Androl<\/i> 2006; 29: 543\u2013552.","DOI":"10.1111\/j.1365-2605.2006.00694.x"},{"key":"17","unstructured":"17. Estienne MJ, Harper AF, Day JL. Characteristics of sperm motility in boar semen diluted in different extenders and stored for seven days at 18 degrees C. Reprod Biol<\/i> 2007; 7: 221\u2013231."},{"key":"18","doi-asserted-by":"crossref","unstructured":"18. Haugan T, Gaustad AH, Reksen O, Gr\u00f6hn YT, Hofmo PO. Fertility results of artificial inseminations performed with liquid boar semen stored in X-cell vs BTS extender. Reprod Domest Anim<\/i> 2007; 42: 94\u201399.","DOI":"10.1111\/j.1439-0531.2006.00738.x"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19. Pinart E, Yeste M, Prieto-Mart\u00ednez N, Reixach J, Bonet S. 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A comparison of wild boar and domestic pig microbiota does not reveal a loss of microbial species but an increase in alpha diversity and opportunistic genera in domestic pigs. Microbiol Spectr<\/i> 2024; 12: e0084324.","DOI":"10.1128\/spectrum.00843-24"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23. Henneberg S, Riedel A, Jung M, Schulze M. Risk factors for Serratia marcescens contamination in liquid preserved boar semen - A cross-laboratory comparative study. Anim Reprod Sci<\/i> 2025; 279: 107955.","DOI":"10.1016\/j.anireprosci.2025.107955"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24. Kuster CE, Althouse GC. The impact of bacteriospermia on boar sperm storage and\u00a0reproductive performance. Theriogenology<\/i> 2016; 85: 21\u201326.","DOI":"10.1016\/j.theriogenology.2015.09.049"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25. Althouse GC. Contaminant toxicity of concern for boars and semen used in assisted reproduction programs. Anim Reprod Sci<\/i> 2024; 269: 107519.","DOI":"10.1016\/j.anireprosci.2024.107519"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26. Sone M, Ohmura K, Bamba K. Effects of various antibiotics on the control of bacteria in boar semen. Vet Rec<\/i> 1982; 111: 11\u201314.","DOI":"10.1136\/vr.111.1.11"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27. Tvrd\u00e1 E, Bu\u010dko O, Rojkov\u00e1 K, \u010eura\u010dka M, Kunov\u00e1 S, Kov\u00e1\u010d J, Benko F, Ka\u010d\u00e1niov\u00e1 M. The efficiency of selected extenders against bacterial contamination of boar semen in a swine breeding facility in Western Slovakia. Animals (Basel)<\/i> 2021; 11: 3320.","DOI":"10.3390\/ani11113320"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28. Luther A-M, Nguyen TQ, Verspohl J, Waberski D. Antimicrobially active semen extenders allow the reduction of antibiotic use in pig insemination. Antibiotics (Basel)<\/i> 2021; 10: 1319.","DOI":"10.3390\/antibiotics10111319"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29. Schulze M, Junkes C, Mueller P, Speck S, Ruediger K, Dathe M, Mueller K. Effects of cationic antimicrobial peptides on liquid-preserved boar spermatozoa. PLoS One<\/i> 2014; 9: e100490.","DOI":"10.1371\/journal.pone.0100490"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30. Speck S, Courtiol A, Junkes C, Dathe M, M\u00fcller K, Schulze M. Cationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen. PLoS One<\/i> 2014; 9: e105949.","DOI":"10.1371\/journal.pone.0105949"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31. Bussalleu E, Sancho S, Briz MD, Yeste M, Bonet S. Do antimicrobial peptides PR-39, PMAP-36 and PMAP-37 have any effect on bacterial growth and quality of liquid-stored boar semen? Theriogenology<\/i> 2017; 89: 235\u2013243.","DOI":"10.1016\/j.theriogenology.2016.11.017"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32. Puig-Timonet A, Castillo-Mart\u00edn M, Pereira BA, Pinart E, Bonet S, Yeste M. Evaluation of porcine beta defensins-1 and -2 as antimicrobial peptides for liquid-stored boar semen: Effects on bacterial growth and sperm quality. Theriogenology<\/i> 2018; 111: 9\u201318.","DOI":"10.1016\/j.theriogenology.2018.01.014"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33. Hensel B, Jakop U, Scheinpflug K, M\u00fchldorfer K, Schr\u00f6ter F, Sch\u00e4fer J, Greber K, Jung M, Schulze M. Low temperature preservation of porcine semen: influence of short antimicrobial lipopeptides on sperm quality and bacterial load. Sci Rep<\/i> 2020; 10: 13225.","DOI":"10.1038\/s41598-020-70180-1"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34. Ros-Santaella JL, Nov\u00fd P, Scaringi M, Pintus E. Antimicrobial peptides and proteins as alternative antibiotics for porcine semen preservation. BMC Vet Res<\/i> 2024; 20: 257.","DOI":"10.1186\/s12917-024-04105-9"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35. Keeratikunakorn K, Chanapiwat P, Aunpad R, Ngamwongsatit N, Kaeoket K. Effect of antimicrobial peptide BiF2_5K7K on contaminated bacteria isolated from boar semen and semen qualities during preservation and subsequent fertility test on pig farm. Antibiotics (Basel)<\/i> 2024; 13: 579.","DOI":"10.3390\/antibiotics13070579"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36. Luther A-M, Varzandeh M, Beckermann C, Feyer L, Maa\u00dfen IK, Oldenhof H, Hackbarth S, Waberski D. Fertility after photodynamic inactivation of bacteria in extended boar semen. Front Microbiol<\/i> 2024; 15: 1429749.","DOI":"10.3389\/fmicb.2024.1429749"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37. Nagahara M, Namula Z, Lin Q, Takebayashi K, Torigoe N, Liu B, Aihara M, Nii M, Otoi T. Effects of curcumin supplementation on quality of porcine spermatozoa irradiated with ultraviolet-C at 228\u2009nm during liquid preservation. Reprod Domest Anim<\/i> 2023; 58: 882\u2013887.","DOI":"10.1111\/rda.14362"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38. Lacalle E, Mart\u00ednez-Mart\u00ednez S, Fern\u00e1ndez-Alegre E, Soriano-\u00dabeda C, Morrell JM, Mart\u00ednez-Pastor F. Low-density colloid centrifugation removes bacteria from boar semen doses after spiking with selected species. Res Vet Sci<\/i> 2023; 158: 215\u2013225.","DOI":"10.1016\/j.rvsc.2023.03.024"},{"key":"39","doi-asserted-by":"crossref","unstructured":"39. Ngo C, Suwimonteerabutr J, Morrell JM, Tummaruk P. Sow reproductive performance following artificial insemination with semen doses processed using Single Layer Centrifugation without antibiotics in the tropics. Theriogenology<\/i> 2024; 226: 194\u2013201.","DOI":"10.1016\/j.theriogenology.2024.06.016"},{"key":"40","doi-asserted-by":"crossref","unstructured":"40. Drago J, Bosch-Ru\u00e9 E, Akrim N, Yeste M, Ribas-Maynou J. Addition of copper chloride and zinc chloride to liquid-stored pig semen reduces bacterial growth without impairing sperm quality. Int J Mol Sci<\/i> 2026; 27: 773.","DOI":"10.3390\/ijms27020773"},{"key":"41","doi-asserted-by":"crossref","unstructured":"41. Ciornei \u0218G, Drugociu D, Ro\u015fca P. Candida genus maximum incidence in boar semen even after preservation, is it not a risk for AI though? Molecules<\/i> 2022; 27: 7539.","DOI":"10.3390\/molecules27217539"},{"key":"42","doi-asserted-by":"crossref","unstructured":"42. Quirino M, Pereira VN, Tamanini MSC, Ulguim RDR, Schulze M, Mellagi APG, Bortolozzo FP. Sperm concentration of boar semen doses and sperm quality: Novel perspectives based on the extender type and sperm resilience. Anim Reprod Sci<\/i> 2023; 255: 107293.","DOI":"10.1016\/j.anireprosci.2023.107293"},{"key":"43","doi-asserted-by":"crossref","unstructured":"43. Wysoki\u0144ska A, Szablicka D, Dzieko\u0144ska A, W\u00f3jcik E. Analysis of changes in the morphological structures of sperm during preservation of liquid boar semen in two different seasons of the year. Anim Reprod Sci<\/i> 2023; 256: 107297.","DOI":"10.1016\/j.anireprosci.2023.107297"},{"key":"44","doi-asserted-by":"crossref","unstructured":"44. Gogol P, Szcze\u015bniak-Fabia\u0144czyk B, Wierzcho\u015b-Hilczer A. The photon emission, ATP level and motility of boar spermatozoa during liquid storage. Reprod Biol<\/i> 2009; 9: 39\u201349.","DOI":"10.1016\/S1642-431X(12)60093-X"},{"key":"45","doi-asserted-by":"crossref","unstructured":"45. Henning H, Nguyen QT, Luther AM, Wallner U, Beyerbach M, Waberski D. In vitro storage of boar spermatozoa increases the demand of adenosine triphosphate for reactivation of motility. Andrology<\/i> 2022; 10: 1426\u20131440.","DOI":"10.1111\/andr.13226"},{"key":"46","doi-asserted-by":"crossref","unstructured":"46. Boe-Hansen GB, Ersb\u00f8ll AK, Greve T, Christensen P. Increasing storage time of extended boar semen reduces sperm DNA integrity. Theriogenology<\/i> 2005; 63: 2006\u20132019.","DOI":"10.1016\/j.theriogenology.2004.09.006"},{"key":"47","doi-asserted-by":"crossref","unstructured":"47. Parrilla I, del Olmo D, Sijses L, Martinez-Alborcia MJ, Cuello C, Vazquez JM, Martinez EA, Roca J. Differences in the ability of spermatozoa from individual boar ejaculates to withstand different semen-processing techniques. Anim Reprod Sci<\/i> 2012; 132: 66\u201373.","DOI":"10.1016\/j.anireprosci.2012.04.003"},{"key":"48","doi-asserted-by":"crossref","unstructured":"48. Kameni SL, Dlamini NH, Feugang JM. Differential metabolomic signatures in boar sperm with varying liquid preservation capacities at 17 \u00b0C. Animals (Basel)<\/i> 2025; 15: 2163.","DOI":"10.3390\/ani15152163"},{"key":"49","doi-asserted-by":"crossref","unstructured":"49. Song C, Zhang Z, Wei Y, Dou Y, Qi K, Li X, Yang F, Li X, Wang K, Qiao R, Han X. Proteomic analysis of boar sperm with differential ability of liquid preservation at 17\u00a0\u00b0C. Theriogenology<\/i> 2024; 215: 103\u2013112.","DOI":"10.1016\/j.theriogenology.2023.11.017"},{"key":"50","doi-asserted-by":"crossref","unstructured":"50. Song C, Chang L, Wang B, Zhang Z, Wei Y, Dou Y, Qi K, Yang F, Li X, Li X, Wang K, Qiao R, Han X. Seminal plasma metabolomics analysis of differences in liquid preservation ability of boar sperm. J Anim Sci<\/i> 2023; 101: skad392.","DOI":"10.1093\/jas\/skad392"},{"key":"51","doi-asserted-by":"crossref","unstructured":"51. Lucca MS, Bustamante-Filho IC, Ulguim RR, Gianluppi RDF, Evaristo JAM, Nogueira FCS, Timmers LFSM, Mellagi APG, Wentz I, Bortolozzo FP. Proteomic analysis of boar seminal plasma: Putative markers for fertility based on capacity of semen preservation at 17\u00b0C. Mol Reprod Dev<\/i> 2024; 91: e23735.","DOI":"10.1002\/mrd.23735"},{"key":"52","doi-asserted-by":"crossref","unstructured":"52. Kou Z, Hu B, Li Y, Cai R, Gao L, Chu G, Yang G, Pang W. Boar seminal plasma improves sperm quality by enhancing its antioxidant capacity during liquid storage at 17\u00b0C. Zygote<\/i> 2022; 30: 695\u2013703.","DOI":"10.1017\/S096719942200017X"},{"key":"53","doi-asserted-by":"crossref","unstructured":"53. Wang Y, Liu Q, Sun Q, Zheng L, Jin T, Cao H, Zhu C, Li L, Gong Y, Yang F, Dong W. Exosomes from porcine serum as endogenous additive maintain function of boar sperm during liquid preservation at 17\u00a0\u00b0C in vitro. Theriogenology<\/i> 2024; 219: 147\u2013156.","DOI":"10.1016\/j.theriogenology.2024.02.015"},{"key":"54","doi-asserted-by":"crossref","unstructured":"54. Huang J, Li S, Yang Y, Li C, Zuo Z, Zheng R, Chai J, Jiang S. GPX5-enriched exosomes improve sperm quality and fertilization ability. Int J Mol Sci<\/i> 2024; 25: 10569.","DOI":"10.3390\/ijms251910569"},{"key":"55","doi-asserted-by":"crossref","unstructured":"55. Chankitisakul V, Saiyamanon H, Boonkum W, Wangkahart E, Ratchamak R. Dose-dependent effects of l-serine supplementation on boar sperm quality during chilled and cryopreserved storage. Animals (Basel)<\/i> 2025; 15: 2670.","DOI":"10.3390\/ani15182670"},{"key":"56","doi-asserted-by":"crossref","unstructured":"56. Zhang H, Qin X, Bojan N, Cao C, Chai J, Pang W. Nicotinamide mononucleotide enhances porcine sperm quality by activating the SIRT3-SOD2\/ROS pathway and promoting oxidative phosphorylation. Anim Reprod Sci<\/i> 2025; 275: 107797.","DOI":"10.1016\/j.anireprosci.2025.107797"},{"key":"57","doi-asserted-by":"crossref","unstructured":"57. Zhang XG, Liu Q, Wang LQ, Yang GS, Hu JH. Effects of glutathione on sperm quality during liquid storage in boars. Anim Sci J<\/i> 2016; 87: 1195\u20131201.","DOI":"10.1111\/asj.12545"},{"key":"58","doi-asserted-by":"crossref","unstructured":"58. Li R, Wu X, Zhu Z, Lv Y, Zheng Y, Lu H, Zhou K, Wu D, Zeng W, Dong W, Zhang T. Polyamines protect boar sperm from oxidative stress in vitro. J Anim Sci<\/i> 2022; 100: skac069.","DOI":"10.1093\/jas\/skac069"},{"key":"59","doi-asserted-by":"crossref","unstructured":"59. Li R, Wu X, Cheng J, Zhu Z, Guo M, Hou G, Li T, Zheng Y, Ma H, Lu H, Chen X, Zhang T, Zeng W. Polyamines protect porcine sperm from lipopolysaccharide-induced mitochondrial dysfunction and apoptosis via casein kinase 2 activation. J Anim Sci<\/i> 2025; 103: skae383.","DOI":"10.1093\/jas\/skae383"},{"key":"60","doi-asserted-by":"crossref","unstructured":"60. Hu Y, Hu J, Pang W, Yang G, Chu G. Astaxanthin improves preserved semen quality at 17\u00b0C by enhancing sperm antioxidant and motility properties. Reprod Domest Anim<\/i> 2022; 57: 1187\u20131197.","DOI":"10.1111\/rda.14190"},{"key":"61","doi-asserted-by":"crossref","unstructured":"61. Lu N, Jiang X, Zhang C, Li B, Tu W, Lei H, Yao W, Xia D. Melatonin mediates via melatonin receptor 1 in a temperature-dependent manner regulating ATP metabolism and antioxidative enzyme activity of boar spermatozoa in\u00a0vitro. Theriogenology<\/i> 2022; 188: 1\u201312.","DOI":"10.1016\/j.theriogenology.2022.05.008"},{"key":"62","doi-asserted-by":"crossref","unstructured":"62. Li Y, Xiao H, Qin X, Zhang H, Zheng Y, Cai R, Pang W. Carboxyfullerene C60 preserves porcine sperm by enhancing antioxidant capacity and inhibiting apoptosis and harmful bacteria. J Anim Sci<\/i> 2024; 102: skae196.","DOI":"10.1093\/jas\/skae196"},{"key":"63","doi-asserted-by":"crossref","unstructured":"63. Katiyar R, Chakravarty H, Deori S, Singh M, Abedin SN, Rautela R, Khargharia G, Mishra VK. Oxyrase-mediated improvement in the quality and fertility of crossbred boar spermatozoa during liquid storage. Reprod Domest Anim<\/i> 2024; 59: e14735.","DOI":"10.1111\/rda.14735"},{"key":"64","doi-asserted-by":"crossref","unstructured":"64. Pantecostoma RR, Jiwakanon J, Sirisathien S. Effect of idebenone supplementation in a semen extender on boar spermatozoa quality during liquid storage. Vet World<\/i> 2025; 18: 1479\u20131486.","DOI":"10.14202\/vetworld.2025.1479-1486"},{"key":"65","doi-asserted-by":"crossref","unstructured":"65. Li C, Liu C, Chen Y, Zhao Y, Tan M, He B. Protective effects of betaine on boar sperm quality during liquid storage and transport. Animals (Basel)<\/i> 2024; 14: 2711.","DOI":"10.3390\/ani14182711"},{"key":"66","doi-asserted-by":"crossref","unstructured":"66. Li C, Oh HJ, Liu H, Kim MK. Schisandrin B protects boar spermatozoa against oxidative damage and increases their fertilization ability during in\u00a0vitro storage. Theriogenology<\/i> 2023; 198: 194\u2013201.","DOI":"10.1016\/j.theriogenology.2022.12.041"},{"key":"67","doi-asserted-by":"crossref","unstructured":"67. Li D, Zhang W, Tian X, He Y, Xiao Z, Zhao X, Fan L, Du R, Yang G, Yu T. Hydroxytyrosol effectively improves the quality of pig sperm at 17\u00a0\u00b0C. Theriogenology<\/i> 2022; 177: 172\u2013182.","DOI":"10.1016\/j.theriogenology.2021.10.018"},{"key":"68","doi-asserted-by":"crossref","unstructured":"68. Li Y, Gaxihamu , Zhang M, Zhang Y, Li W, Du J, Qiu R, Chen H. The effect of sodium phytate on the liquid preservation of porcine semen. Reprod Domest Anim<\/i> 2025; 60: e70089.","DOI":"10.1111\/rda.70089"},{"key":"69","doi-asserted-by":"crossref","unstructured":"69. Liu W, Li W, Lin R, Wang Y, Zhou P, Pang W, Li R, Zheng Y. Arbutin preserves boar sperm during storage at 17\u00b0C by enhancing the antioxidant capacity via the NRF2\/GPX4 signalling. Reprod Domest Anim<\/i> 2025; 60: e70103.","DOI":"10.1111\/rda.70103"},{"key":"70","doi-asserted-by":"crossref","unstructured":"70. Cao C, Qin X, Sha Y, Xiao H, Lai Z, Zheng Y, Cai R, Pang W. Vitamin E nanoemulsion enhances porcine sperm quality and reproductive performance via antioxidant enhancement and fatty acid metabolism regulation. Anim Reprod Sci<\/i> 2025; 279: 107954.","DOI":"10.1016\/j.anireprosci.2025.107954"},{"key":"71","doi-asserted-by":"crossref","unstructured":"71. Restrepo G, Zapata K, Colorado P, Rojano B. Cooling of porcine semen in an extender supplemented with carvacrol. Reprod Domest Anim<\/i> 2023; 58: 860\u2013866.","DOI":"10.1111\/rda.14359"},{"key":"72","doi-asserted-by":"crossref","unstructured":"72. Shepherd MJ, Minton AM, Schwab C, Herickhoff LA. Effects of boar sperm antioxidant supplementation on fertility. Anim Reprod Sci<\/i> 2022; 237: 106923.","DOI":"10.1016\/j.anireprosci.2022.106923"},{"key":"73","doi-asserted-by":"crossref","unstructured":"73. Zapparoli H, Pedrosa AC, Pradiee J, da Silva GF, Nieto RDP, Cabral LMC, Dos Santos Gomes F, Tonon RV, Martins SMMK, de Andrade AFC. Effect of the simultaneous addition of cyanidin 3-glucoside and cyanidin 3-rutinoside in boar semen preserved under refrigeration at 17\u00b0C for 168\u202fhours. Anim Reprod Sci<\/i> 2025; 276: 107799.","DOI":"10.1016\/j.anireprosci.2025.107799"},{"key":"74","doi-asserted-by":"crossref","unstructured":"74. Jawad A, Oh D, Choi H, Kim M, Cai L, Lee J, Hyun S-H. Myo-inositol improves the viability of boar sperm during liquid storage. Front Vet Sci<\/i> 2023; 10: 1150984.","DOI":"10.3389\/fvets.2023.1150984"},{"key":"75","doi-asserted-by":"crossref","unstructured":"75. Wang S, Wang Q, Zhang H, Zeng X, Min L, Adetunji AO, Zhu Z. Succinic acid improves boar semen quality by reducing ROS stress during liquid preservation. Theriogenology<\/i> 2026; 251: 117757.","DOI":"10.1016\/j.theriogenology.2025.117757"},{"key":"76","doi-asserted-by":"crossref","unstructured":"76. Hu B, Zhang H, Li Y, Xue Q, Yang M, Cao C, Gao L, Chu G, Cai R, Zheng Y, Pang W. Kojic acid inhibits pig sperm apoptosis and improves capacitated sperm state during liquid preservation at 17\u00b0C. Mol Reprod Dev<\/i> 2024; 91: e23738.","DOI":"10.1002\/mrd.23738"},{"key":"77","doi-asserted-by":"crossref","unstructured":"77. Garriga F, Mart\u00ednez-Hern\u00e1ndez J, Gener-Velasco N, Rodr\u00edguez-Gil JE, Yeste M. Voltage-dependent anion channels are involved in the maintenance of pig sperm quality during liquid preservation. Theriogenology<\/i> 2024; 224: 26\u201333.","DOI":"10.1016\/j.theriogenology.2024.05.003"},{"key":"78","doi-asserted-by":"crossref","unstructured":"78. Garriga F, Maside C, Padilla L, Recuero S, Rodr\u00edguez-Gil JE, Yeste M. Heat shock protein 70\u00a0kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17\u00a0\u00b0C. Sci Rep<\/i> 2024; 14: 13383.","DOI":"10.1038\/s41598-024-64488-5"},{"key":"79","doi-asserted-by":"crossref","unstructured":"79. Guo Z, Zhang Y, Huang A, Ni Q, Zeng C. Phenylbutyrate and Dichloroacetate Enhance the Liquid-Stored Boar Sperm Quality via PDK1 and PDK3. Int J Mol Sci<\/i> 2023; 24: 17091.","DOI":"10.3390\/ijms242317091"},{"key":"80","doi-asserted-by":"crossref","unstructured":"80. Garriga F, Codina-Benaiges J, Yeste M, Llavanera M. Calcium homeostasis role in preserving sperm function and metabolic activity during liquid storage of pig semen. Theriogenology<\/i> 2026; 249: 117638.","DOI":"10.1016\/j.theriogenology.2025.117638"},{"key":"81","doi-asserted-by":"crossref","unstructured":"81. Lee SH, Park CK. Effect of magnetized extender on sperm membrane integrity and development of oocytes in vitro fertilized with liquid storage boar semen. Anim Reprod Sci<\/i> 2015; 154: 86\u201394.","DOI":"10.1016\/j.anireprosci.2014.12.015"},{"key":"82","doi-asserted-by":"crossref","unstructured":"82. Schmid S, Henning H, Petrunkina AM, Weitze KF, Waberski D. Response to capacitating stimuli indicates extender-related differences in boar sperm function. J Anim Sci<\/i> 2013; 91: 5018\u20135025.","DOI":"10.2527\/jas.2013-6287"},{"key":"83","doi-asserted-by":"crossref","unstructured":"83. Waberski D, Luther A-M. Boar semen storage at 5 \u00b0C for the reduction of antibiotic use in pig insemination: Pathways from science into practice. Anim Reprod Sci<\/i> 2024; 269: 107486.","DOI":"10.1016\/j.anireprosci.2024.107486"},{"key":"84","doi-asserted-by":"crossref","unstructured":"84. Luther A-M, Nguyen TQ, Verspohl J, Waberski D. Update of the cooling protocol for antibiotic-free storage of boar semen at 5\u00b0C improves sperm quality and maintains low bacterial counts. PLoS One<\/i> 2024; 19: e0305280.","DOI":"10.1371\/journal.pone.0305280"},{"key":"85","doi-asserted-by":"crossref","unstructured":"85. Grudzinska P, Luongo C, Quintero-Moreno A, Garc\u00eda-V\u00e1zquez FA. Implications of different hypothermic preservation conditions on boar sperm quality during storage: Evaluation in sperm-rich fraction and whole ejaculate. Res Vet Sci<\/i> 2025; 197: 105936.","DOI":"10.1016\/j.rvsc.2025.105936"},{"key":"86","doi-asserted-by":"crossref","unstructured":"86. Hensel B, Pieper L, Jung M, Schulze M. Influence of age, breed, and season on the quality of boar semen stored at low-temperature. Theriogenology<\/i> 2023; 208: 102\u2013108.","DOI":"10.1016\/j.theriogenology.2023.06.010"},{"key":"87","doi-asserted-by":"crossref","unstructured":"87. Maa\u00dfen IK, Luther A-M, Verspohl J, Waberski D. Storage of extended boar semen at 5 \u00b0C inhibits growth of multi-drug resistant Serratia marcescens<\/i> and Klebsiella oxytoca<\/i> while maintaining high sperm quality. Antibiotics (Basel)<\/i> 2023; 12: 857.","DOI":"10.3390\/antibiotics12050857"},{"key":"88","doi-asserted-by":"crossref","unstructured":"88. Reckinger F, Luther A-M, Verspohl J, Artavia JL, Waberski D. Pre-cooling of boar semen before transport in a hot environment enhances biosafety. Front Microbiol<\/i> 2025; 16: 1611562.","DOI":"10.3389\/fmicb.2025.1611562"},{"key":"89","doi-asserted-by":"crossref","unstructured":"89. Gonzalez-Castro RA, Pe\u00f1a FJ, Herickhoff LA. Spermatozoa cooled to 5\u00b0C one\u00a0day after collection from porcine commercial semen doses retain sperm functionality with reduced bacterial load. Andrology<\/i> 2024; 12: 186\u2013197.","DOI":"10.1111\/andr.13441"},{"key":"90","doi-asserted-by":"crossref","unstructured":"90. Jochems R, Kristiansen CO, Kommisrud E, Morrell JM. Preliminary study of pregnancy rates and litter sizes following artificial insemination of boar spermatozoa prepared by colloid centrifugation and hypothermic storage. Sci Rep<\/i> 2025; 15: 40863.","DOI":"10.1038\/s41598-025-24586-4"},{"key":"91","doi-asserted-by":"crossref","unstructured":"91. Yang D, Yu X, Li X, Yu B, Peng H. Protective effects of l-cysteine and N-acetyl-l-cysteine on boar sperm quality during hypothermic liquid storage with bovine serum albumin as a protectant. Theriogenology<\/i> 2024; 216: 185\u2013195.","DOI":"10.1016\/j.theriogenology.2023.12.030"},{"key":"92","doi-asserted-by":"crossref","unstructured":"92. dos Santos G, Tamanini MSC, Leal LA, Wolf LM, Christ TS, Piton YV, Arbo MD, Bernardi ML, Ulguim RDR, Bortolozzo FP, Mellagi APG. L-cysteine improves boar semen motility at 5 \u00ba<\/sup>C but does not affect the oxidative status. Anim Reprod Sci<\/i> 2024; 260: 107384.","DOI":"10.1016\/j.anireprosci.2023.107384"},{"key":"93","doi-asserted-by":"crossref","unstructured":"93. Wang L, Xiong M, Li S, Ma S, Jiang S, Wang H, Zhang J, Li X. Beneficial effects of EGCG on boar sperm quality during liquid storage at 4\u00a0\u00b0C are mediated by DRD2 receptor. Theriogenology<\/i> 2025; 234: 174\u2013185.","DOI":"10.1016\/j.theriogenology.2024.12.019"},{"key":"94","doi-asserted-by":"crossref","unstructured":"94. Schulze M, R\u00fcdiger K, Waberski D. Rotation of boar semen doses during storage affects sperm quality. Reprod Domest Anim<\/i> 2015; 50: 684\u2013687.","DOI":"10.1111\/rda.12532"},{"key":"95","doi-asserted-by":"crossref","unstructured":"95. Paschoal AFL, Luther A-M, Jakop U, Schulze M, Bortolozzo FP, Waberski D. Factors influencing the response of spermatozoa to agitation stress: Implications for transport of extended boar semen. Theriogenology<\/i> 2021; 175: 54\u201360.","DOI":"10.1016\/j.theriogenology.2021.08.033"},{"key":"96","doi-asserted-by":"crossref","unstructured":"96. Hensel B, Henneberg S, Riesenbeck A, Jung M, Schulze M. Effects of vibrations during boar semen transport: Low-temperature transport as a new management tool. Anim Reprod Sci<\/i> 2024; 261: 107413.","DOI":"10.1016\/j.anireprosci.2024.107413"},{"key":"97","doi-asserted-by":"crossref","unstructured":"97. Tamanini MSC, Dos Santos G, Leal LA, Wolf LM, Schulze M, Christ TS, Bortolozzo FP, Ulguim RR, Wentz I, Mellagi APG. Impact of agitation time of boar semen doses on sperm traits in short- and long-term extenders. Anim Reprod Sci<\/i> 2022; 247: 107159.","DOI":"10.1016\/j.anireprosci.2022.107159"},{"key":"98","doi-asserted-by":"crossref","unstructured":"98. Hensel B, Schulze M. Transport-related influences on the quality of liquid-preserved boar sperm: A review. Anim Reprod Sci<\/i> 2025; 272: 107657.","DOI":"10.1016\/j.anireprosci.2024.107657"},{"key":"99","doi-asserted-by":"crossref","unstructured":"99. Bona GA, Rech RD, Leal LA, Wolf LM, Christ TS, Schulze M, Bortolozzo FP, Ulguim RR, Mellagi APG. Influence of vibration duration on the quality of boar semen doses packed in bags or tubes. Anim Reprod Sci<\/i> 2025; 272: 107658.","DOI":"10.1016\/j.anireprosci.2024.107658"},{"key":"100","doi-asserted-by":"crossref","unstructured":"100. Yeste M. Recent advances in boar sperm cryopreservation: state of the art and current perspectives. Reprod Domest Anim<\/i> 2015; 50(Suppl 2): 71\u201379.","DOI":"10.1111\/rda.12569"},{"key":"101","doi-asserted-by":"crossref","unstructured":"101. Yeste M. Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenology<\/i> 2016; 85: 47\u201364.","DOI":"10.1016\/j.theriogenology.2015.09.047"},{"key":"102","doi-asserted-by":"crossref","unstructured":"102. Y\u00e1nez-Ortiz I, Catal\u00e1n J, Rodr\u00edguez-Gil JE, Mir\u00f3 J, Yeste M. Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep. Anim Reprod Sci<\/i> 2022; 246: 106904.","DOI":"10.1016\/j.anireprosci.2021.106904"},{"key":"103","doi-asserted-by":"crossref","unstructured":"103. \u00c1lvarez-Rodr\u00edguez M, Tom\u00e1s-Almenar C, Nieto-Crist\u00f3bal H, de Mercado E. Evaluation of different thawing protocols on iberian boar sperm preserved for 10 years at different liquid nitrogen levels. Animals (Basel)<\/i> 2024; 14: 914.","DOI":"10.3390\/ani14060914"},{"key":"104","doi-asserted-by":"crossref","unstructured":"104. Bolarin A, Berndtson J, Tejerina F, Cobos S, Pomarino C, D\u2019Alessio F, Blackburn H, Kaeoket K. Boar semen cryopreservation: State of the art, and international trade vision. Anim Reprod Sci<\/i> 2024; 269: 107496.","DOI":"10.1016\/j.anireprosci.2024.107496"},{"key":"105","doi-asserted-by":"crossref","unstructured":"105. Delgado-Berm\u00fadez A. Insights into crucial molecules and protein channels involved in pig sperm cryopreservation. Anim Reprod Sci<\/i> 2024; 269: 107547.","DOI":"10.1016\/j.anireprosci.2024.107547"},{"key":"106","doi-asserted-by":"crossref","unstructured":"106. Monteiro MS, Torres MA, Passarelli MDS, Martins MP, Ravagnani GM, Papa FO, Alvarenga MA, Dell\u2019Aqua J\u00fanior JA, Yasui GS, Martins SMMK, de Andrade AFC. Impact of cryopreservation protocols (one- and two-step) on boar semen quality at 5\u00a0\u00b0C and post-thawing. Anim Reprod Sci<\/i> 2022; 247: 107093.","DOI":"10.1016\/j.anireprosci.2022.107093"},{"key":"107","doi-asserted-by":"crossref","unstructured":"107. Passarelli MDS, Pinoti Pavaneli AP, Mouro Ravagnani G, Pasini Martins M, Pedrosa AC, Maria Massami Kitamura Martins S, Rocha NRA, Bittar Rigo VH, Yasui G, Yeste M, de Andrade AFC. Effects of different equilibration times at 5\u202f\u00b0C on boar sperm cryotolerance. Anim Reprod Sci<\/i> 2020; 219: 106547.","DOI":"10.1016\/j.anireprosci.2020.106547"},{"key":"108","doi-asserted-by":"crossref","unstructured":"108. Shepherd MJ, Herickhoff LA. A novel experimental design for boar sperm cryopreservation. J Anim Sci<\/i> 2022; 100: skac169.","DOI":"10.1093\/jas\/skac169"},{"key":"109","doi-asserted-by":"crossref","unstructured":"109. Yeste M, Estrada E, Rivera Del \u00c1lamo M-M, Bonet S, Rigau T, Rodr\u00edguez-Gil J-E. The increase in phosphorylation levels of serine residues of protein HSP70 during holding time at 17\u00b0C is concomitant with a higher cryotolerance of boar spermatozoa. PLoS One<\/i> 2014; 9: e90887.","DOI":"10.1371\/journal.pone.0090887"},{"key":"110","doi-asserted-by":"crossref","unstructured":"110. Torres MA, Pedrosa AC, Novais FJ, Alkmin DV, Cooper BR, Yasui GS, Fukumasu H, Machaty Z, de Andrade AFC. Metabolomic signature of spermatozoa established during holding time is responsible for differences in boar sperm freezability. Biol Reprod<\/i> 2022; 106: 213\u2013226.","DOI":"10.1093\/biolre\/ioab200"},{"key":"111","doi-asserted-by":"crossref","unstructured":"111. Torres P, Malcervelli DM, Cartelle JH, Fischman ML, Cisale HO. Cold-induced damage in boar sperm cooled to 5\u00b0C in Lactose Egg Yolk (LEY) medium with different Glycerol concentrations. Anim Reprod Sci<\/i> 2024; 264: 107455.","DOI":"10.1016\/j.anireprosci.2024.107455"},{"key":"112","doi-asserted-by":"crossref","unstructured":"112. Kim S, Lee Y-J, Ji D-B, Kim Y-J. Evaluation of Different Cryoprotectants (CPAs) in Boar Semen Cryopreservation. vol. 73. 2011.","DOI":"10.1292\/jvms.10-0345"},{"key":"113","doi-asserted-by":"crossref","unstructured":"113. Kong Y, He M, Gao J, Xu J, Lu N, Wu C, Sun L, Dai J. Comparative analysis of classic semen extenders for frozen-thawed boar semen. Animals (Basel)<\/i> 2025; 15: 1885.","DOI":"10.3390\/ani15131885"},{"key":"114","doi-asserted-by":"crossref","unstructured":"114. De Andrade A, Grossfeld R, Knox RV. In vitro effects of two different commercial freezing and thawing extenders on boar sperm quality. Anim Reprod Sci<\/i> 2022; 236: 106906.","DOI":"10.1016\/j.anireprosci.2021.106906"},{"key":"115","doi-asserted-by":"crossref","unstructured":"115. Jiang ZL, Li QW, Hu JH, Li WY, Zhao HW, Zhang SS. Improvement of the quality of boar cryopreservation semen by supplementing with low density lipoprotein in diluents. Cryobiology<\/i> 2007; 54: 301\u2013304.","DOI":"10.1016\/j.cryobiol.2007.03.001"},{"key":"116","doi-asserted-by":"crossref","unstructured":"116. Hu JH, Li QW, Jiang ZL, Li WY. Effects of different extenders on DNA integrity of boar spermatozoa following freezing-thawing. Cryobiology<\/i> 2008; 57: 257\u2013262.","DOI":"10.1016\/j.cryobiol.2008.09.004"},{"key":"117","doi-asserted-by":"crossref","unstructured":"117. Vadnais ML, Althouse GC. Characterization of capacitation, cryoinjury, and the role of seminal plasma in porcine sperm. Theriogenology<\/i> 2011; 76: 1508\u20131516.","DOI":"10.1016\/j.theriogenology.2011.06.021"},{"key":"118","doi-asserted-by":"crossref","unstructured":"118. Yeste M, Flores E, Estrada E, Bonet S, Rigau T, Rodr\u00edguez-Gil JE. Reduced glutathione and procaine hydrochloride protect the nucleoprotein structure of boar spermatozoa during freeze-thawing by stabilising disulfide bonds. Reprod Fertil Dev<\/i> 2013; 25: 1036\u20131050.","DOI":"10.1071\/RD12230"},{"key":"119","doi-asserted-by":"crossref","unstructured":"119. Estrada E, Rodr\u00edguez-Gil JE, Rocha LG, Balasch S, Bonet S, Yeste M. Supplementing cryopreservation media with reduced glutathione increases fertility and prolificacy of sows inseminated with frozen-thawed boar semen. Andrology<\/i> 2014; 2: 88\u201399.","DOI":"10.1111\/j.2047-2927.2013.00144.x"},{"key":"120","doi-asserted-by":"crossref","unstructured":"120. Yeste M, Estrada E, Pinart E, Bonet S, Mir\u00f3 J, Rodr\u00edguez-Gil JE. The improving effect of reduced glutathione on boar sperm cryotolerance is related with the intrinsic ejaculate freezability. Cryobiology<\/i> 2014; 68: 251\u2013261.","DOI":"10.1016\/j.cryobiol.2014.02.004"},{"key":"121","doi-asserted-by":"crossref","unstructured":"121. Yeste M, Estrada E, Casas I, Bonet S, Rodr\u00edguez-Gil JE. Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels. Theriogenology<\/i> 2013; 79: 929\u2013939.","DOI":"10.1016\/j.theriogenology.2013.01.008"},{"key":"122","doi-asserted-by":"crossref","unstructured":"122. Alkmin DV, Martinez-Alborcia MJ, Parrilla I, Vazquez JM, Martinez EA, Roca J. The nuclear DNA longevity in cryopreserved boar spermatozoa assessed using the Sperm-Sus-Halomax. Theriogenology<\/i> 2013; 79: 1294\u20131300.","DOI":"10.1016\/j.theriogenology.2013.02.026"},{"key":"123","doi-asserted-by":"crossref","unstructured":"123. Flores E, Rami\u00f3-Lluch L, Bucci D, Fern\u00e1ndez-Novell JM, Pe\u00f1a A, Rodr\u00edguez-Gil JE. Freezing-thawing induces alterations in histone H1-DNA binding and the breaking of protein-DNA disulfide bonds in boar sperm. Theriogenology<\/i> 2011; 76: 1450\u20131464.","DOI":"10.1016\/j.theriogenology.2011.05.039"},{"key":"124","doi-asserted-by":"crossref","unstructured":"124. Awda BJ, Mackenzie-Bell M, Buhr MM. Reactive oxygen species and boar sperm function. Biol Reprod<\/i> 2009; 81: 553\u2013561.","DOI":"10.1095\/biolreprod.109.076471"},{"key":"125","doi-asserted-by":"crossref","unstructured":"125. Guthrie HD, Welch GR. Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry. J Anim Sci<\/i> 2006; 84: 2089\u20132100.","DOI":"10.2527\/jas.2005-766"},{"key":"126","doi-asserted-by":"crossref","unstructured":"126. Kim S, Lee Y-J, Kim Y-J. Changes in sperm membrane and ROS following cryopreservation of liquid boar semen stored at 15 \u00b0C. Anim Reprod Sci<\/i> 2011; 124: 118\u2013124.","DOI":"10.1016\/j.anireprosci.2011.01.014"},{"key":"127","doi-asserted-by":"crossref","unstructured":"127. Flores E, Fern\u00e1ndez-Novell JM, Pe\u00f1a A, Rigau T, Rodr\u00edguez-Gil JE. Cryopreservation-induced alterations in boar spermatozoa mitochondrial function are related to changes in the expression and location of midpiece mitofusin-2 and actin network. Theriogenology<\/i> 2010; 74: 354\u2013363.","DOI":"10.1016\/j.theriogenology.2010.02.018"},{"key":"128","doi-asserted-by":"crossref","unstructured":"128. Sancho S, Casas I, Ekwall H, Saravia F, Rodriguez-Martinez H, Rodriguez-Gil JE, Flores E, Pinart E, Briz M, Garcia-Gil N, Bassols J, Pruneda A, Bussalleu E, Yeste M, Bonet S. Effects of cryopreservation on semen quality and the expression of sperm membrane hexose transporters in the spermatozoa of Iberian pigs. Reproduction<\/i> 2007; 134: 111\u2013121.","DOI":"10.1530\/REP-07-0118"},{"key":"129","doi-asserted-by":"crossref","unstructured":"129. Flores E, Cifuentes D, Fern\u00e1ndez-Novell JM, Medrano A, Bonet S, Briz MD, Pinart E, Pe\u00f1a A, Rigau T, Rodr\u00edguez-Gil JE. Freeze-thawing induces alterations in the protamine-1\/DNA overall structure in boar sperm. Theriogenology<\/i> 2008; 69: 1083\u20131094.","DOI":"10.1016\/j.theriogenology.2008.01.022"},{"key":"130","doi-asserted-by":"crossref","unstructured":"130. Kumaresan A, Siqueira AP, Hossain MS, Bergqvist AS. Cryopreservation-induced alterations in protein tyrosine phosphorylation of spermatozoa from different portions of the boar ejaculate. Cryobiology<\/i> 2011; 63: 137\u2013144.","DOI":"10.1016\/j.cryobiol.2011.08.002"},{"key":"131","doi-asserted-by":"crossref","unstructured":"131. Ali MA, Qin Z, Dou S, Huang A, Wang Y, Yuan X, Zhang Y, Ni Q, Azmat R, Zeng C. Cryopreservation induces acetylation of metabolism-related proteins in boar sperm. Int J Mol Sci<\/i> 2023; 24: 10983.","DOI":"10.3390\/ijms241310983"},{"key":"132","doi-asserted-by":"crossref","unstructured":"132. Zhang Y, Chen X, Cao L, Zhang J, Wang J, Yao Z, Zhao K, Jin Y. SUMO1 modification reduces oxidative stress and SUMO1ylated AKAP4 degradation affects frozen-thawed boar sperm quality. Anim Reprod Sci<\/i> 2025; 273: 107759.","DOI":"10.1016\/j.anireprosci.2024.107759"},{"key":"133","doi-asserted-by":"crossref","unstructured":"133. Parrilla I, Cambra JM, Cuello C, Rodriguez-Martinez H, Gil MA, Martinez EA. Cryopreservation of highly extended pig spermatozoa remodels its proteome and counteracts polyspermic fertilization in vitro. Andrology<\/i> 2024; 12: 1356\u20131372.","DOI":"10.1111\/andr.13575"},{"key":"134","doi-asserted-by":"crossref","unstructured":"134. Zeng C, Tang K, He L, Peng W, Ding L, Fang D, Zhang Y. Effects of glycerol on apoptotic signaling pathways during boar spermatozoa cryopreservation. Cryobiology<\/i> 2014; 68: 395\u2013404.","DOI":"10.1016\/j.cryobiol.2014.03.008"},{"key":"135","doi-asserted-by":"crossref","unstructured":"135. Knox RV. The fertility of frozen boar sperm when used for artificial insemination. Reprod Domest Anim<\/i> 2015; 50(Suppl 2): 90\u201397.","DOI":"10.1111\/rda.12552"},{"key":"136","doi-asserted-by":"crossref","unstructured":"136. Casas I, Sancho S, Briz M, Pinart E, Bussalleu E, Yeste M, Bonet S. Fertility after post-cervical artificial insemination with cryopreserved sperm from boar ejaculates of good and poor freezability. Anim Reprod Sci<\/i> 2010; 118: 69\u201376.","DOI":"10.1016\/j.anireprosci.2009.06.003"},{"key":"137","doi-asserted-by":"crossref","unstructured":"137. Bathgate R, Eriksson BM, Thomson PC, Maxwell WMC, Evans G. Field fertility of frozen-thawed boar sperm at low doses using non-surgical, deep uterine insemination. Anim Reprod Sci<\/i> 2008; 103: 323\u2013335.","DOI":"10.1016\/j.anireprosci.2007.01.008"},{"key":"138","doi-asserted-by":"crossref","unstructured":"138. Spencer KW, Purdy PH, Blackburn HD, Spiller SF, Stewart TS, Knox RV. Effect of number of motile, frozen-thawed boar sperm and number of fixed-time inseminations on fertility in estrous-synchronized gilts. Anim Reprod Sci<\/i> 2010; 121: 259\u2013266.","DOI":"10.1016\/j.anireprosci.2010.07.002"},{"key":"139","doi-asserted-by":"crossref","unstructured":"139. Fraser L, Zasiadczyk \u0141, Mogielnicka-Brzozowska M. Boar-to-boar variations in quality characteristics of sperm from different ejaculates following freezing-thawing. Cells<\/i> 2025; 14: 212.","DOI":"10.3390\/cells14030212"},{"key":"140","doi-asserted-by":"crossref","unstructured":"140. Casas I, Sancho S, Briz M, Pinart E, Bussalleu E, Yeste M, Bonet S. Freezability prediction of boar ejaculates assessed by functional sperm parameters and sperm proteins. Theriogenology<\/i> 2009; 72: 930\u2013948.","DOI":"10.1016\/j.theriogenology.2009.07.001"},{"key":"141","doi-asserted-by":"crossref","unstructured":"141. Casas I, Sancho S, Ballester J, Briz M, Pinart E, Bussalleu E, Yeste M, F\u00e0brega A, Rodr\u00edguez-Gil JE, Bonet S. The HSP90AA1 sperm content and the prediction of the boar ejaculate freezability. Theriogenology<\/i> 2010; 74: 940\u2013950.","DOI":"10.1016\/j.theriogenology.2010.04.021"},{"key":"142","doi-asserted-by":"crossref","unstructured":"142. Vilagran I, Castillo J, Bonet S, Sancho S, Yeste M, Estanyol JM, Oliva R. Acrosin-binding protein (ACRBP) and triosephosphate isomerase (TPI) are good markers to predict boar sperm freezing capacity. Theriogenology<\/i> 2013; 80: 443\u2013450.","DOI":"10.1016\/j.theriogenology.2013.05.006"},{"key":"143","doi-asserted-by":"crossref","unstructured":"143. Vilagran I, Yeste M, Sancho S, Casas I, Rivera del \u00c1lamo MM, Bonet S. Relationship of sperm small heat-shock protein 10 and voltage-dependent anion channel 2 with semen freezability in boars. Theriogenology<\/i> 2014; 82: 418\u2013426.","DOI":"10.1016\/j.theriogenology.2014.04.023"},{"key":"144","doi-asserted-by":"crossref","unstructured":"144. Prieto-Mart\u00ednez N, Vilagran I, Morat\u00f3 R, Rivera Del \u00c1lamo MM, Rodr\u00edguez-Gil JE, Bonet S, Yeste M. Relationship of aquaporins 3 (AQP3), 7 (AQP7), and 11 (AQP11) with boar sperm resilience to withstand freeze-thawing procedures. Andrology<\/i> 2017; 5: 1153\u20131164.","DOI":"10.1111\/andr.12410"},{"key":"145","doi-asserted-by":"crossref","unstructured":"145. Delgado-Berm\u00fadez A, Llavanera M, Fern\u00e1ndez-Bastit L, Recuero S, Mateo-Otero Y, Bonet S, Barranco I, Fern\u00e1ndez-Fuertes B, Yeste M. Aquaglyceroporins but not orthodox aquaporins are involved in the cryotolerance of pig spermatozoa. J Anim Sci Biotechnol<\/i> 2019; 10: 77.","DOI":"10.1186\/s40104-019-0388-8"},{"key":"146","doi-asserted-by":"crossref","unstructured":"146. Delgado-Berm\u00fadez A, Llavanera M, Recuero S, Mateo-Otero Y, Bonet S, Barranco I, Fernandez-Fuertes B, Yeste M. Effect of AQP inhibition on boar sperm cryotolerance depends on the intrinsic freezability of the ejaculate. Int J Mol Sci<\/i> 2019; 20: 6255.","DOI":"10.3390\/ijms20246255"},{"key":"147","doi-asserted-by":"crossref","unstructured":"147. Delgado-Berm\u00fadez A, Mateo-Otero Y, Llavanera M, Bonet S, Yeste M, Pinart E. Hvcn1 but not potassium channels are related to mammalian sperm cryotolerance. Int J Mol Sci<\/i> 2021; 22: 1646.","DOI":"10.3390\/ijms22041646"},{"key":"148","doi-asserted-by":"crossref","unstructured":"148. Llavanera M, Delgado-Berm\u00fadez A, Fernandez-Fuertes B, Recuero S, Mateo Y, Bonet S, Barranco I, Yeste M. GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm. J Anim Sci Biotechnol<\/i> 2019; 10: 61.","DOI":"10.1186\/s40104-019-0370-5"},{"key":"149","doi-asserted-by":"crossref","unstructured":"149. Tong S, Yin C, Ge Y, Ren Z, Tao J, Liu Y. Albumin (ALB) and protein disulfide isomerase family A member 4 (PDIA4) are novel markers to predict sperm freezability of Erhualian boar. Cryobiology<\/i> 2022; 109: 37\u201343.","DOI":"10.1016\/j.cryobiol.2022.09.006"},{"key":"150","doi-asserted-by":"crossref","unstructured":"150. Ma\u0144kowska A, Gilun P, Zasiadczyk \u0141, Sobiech P, Fraser L. Expression of TXNRD1, HSPA4L<\/i> and ATP1B1<\/i> Genes Associated with the Freezability of Boar Sperm. Int J Mol Sci<\/i> 2022; 23: 9320.","DOI":"10.3390\/ijms23169320"},{"key":"151","doi-asserted-by":"crossref","unstructured":"151. Pinart E, Yeste M, Bonet S. Acrosin activity is a good predictor of boar sperm freezability. Theriogenology<\/i> 2015; 83: 1525\u20131533.","DOI":"10.1016\/j.theriogenology.2015.02.005"},{"key":"152","doi-asserted-by":"crossref","unstructured":"152. Estrada E, Rodr\u00edguez-Gil JE, Rivera Del \u00c1lamo MM, Pe\u00f1a A, Yeste M. Effects of reduced glutathione on acrosin activity in frozen-thawed boar spermatozoa. Reprod Fertil Dev<\/i> 2017; 29: 283\u2013293.","DOI":"10.1071\/RD15118"},{"key":"153","doi-asserted-by":"crossref","unstructured":"153. Vilagran I, Yeste M, Sancho S, Castillo J, Oliva R, Bonet S. Comparative analysis of boar seminal plasma proteome from different freezability ejaculates and identification of Fibronectin 1 as sperm freezability marker. Andrology<\/i> 2015; 3: 345\u2013356.","DOI":"10.1111\/andr.12009"},{"key":"154","doi-asserted-by":"crossref","unstructured":"154. Wysocki P, Orzo\u0142ek A, Strze\u017cek J, Koziorowska-Gilun M, Zasiadczyk \u0141, Kordan W. The activity of N-acetyl-\u03b2-hexosaminidase in boar seminal plasma is linked with semen quality and its suitability for cryopreservation. Theriogenology<\/i> 2015; 83: 1194\u20131202.","DOI":"10.1016\/j.theriogenology.2014.12.025"},{"key":"155","doi-asserted-by":"crossref","unstructured":"155. Wang K, Jiao H, Cheng X, Zhang L, Zhang S, Liu G, Meng F, Zhan F, Yang F. Proteomic analysis of differences in the freezability of porcine sperm identifies \u03b1-amylase as a key protein. J Proteome Res<\/i> 2024; 23: 2641\u20132650.","DOI":"10.1021\/acs.jproteome.4c00367"},{"key":"156","doi-asserted-by":"crossref","unstructured":"156. Wang W, Bai Y, Wang X, Zhang J, Li B, Zhang H, Zhou X, Wang H, Liu B. Identification of key genes and variants associated with boar sperm freezability using whole genome resequencing. Int J Biol Macromol<\/i> 2025; 294: 139268.","DOI":"10.1016\/j.ijbiomac.2024.139268"},{"key":"157","doi-asserted-by":"crossref","unstructured":"157. Ma\u0144kowska A, Brym P, Sobiech P, Fraser L. Promoter polymorphisms in STK35 and IFT27 genes and their associations with boar sperm freezability. Theriogenology<\/i> 2022; 189: 199\u2013208.","DOI":"10.1016\/j.theriogenology.2022.06.023"},{"key":"158","doi-asserted-by":"crossref","unstructured":"158. Li M, Bai Y, Zhang J, Wang H, Li J, Wang W. Sperm metabolomics identifies freezability markers in Duroc, Landrace, and Large White boars. Theriogenology<\/i> 2025; 240: 117395.","DOI":"10.1016\/j.theriogenology.2025.117395"},{"key":"159","doi-asserted-by":"crossref","unstructured":"159. Hern\u00e1ndez M, Roca J, Calvete JJ, Sanz L, Mui\u00f1o-Blanco T, Cebri\u00e1n-P\u00e9rez JA, V\u00e1zquez JM, Mart\u00ednez EA. Cryosurvival and in vitro fertilizing capacity postthaw is improved when boar spermatozoa are frozen in the presence of seminal plasma from good freezer boars. J Androl<\/i> 2007; 28: 689\u2013697.","DOI":"10.2164\/jandrol.107.002725"},{"key":"160","doi-asserted-by":"crossref","unstructured":"160. Li J, Barranco I, Tvarijonaviciute A, Molina MF, Martinez EA, Rodriguez-Martinez H, Parrilla I, Roca J. Seminal plasma antioxidants are directly involved in boar sperm cryotolerance. Theriogenology<\/i> 2018; 107: 27\u201335.","DOI":"10.1016\/j.theriogenology.2017.10.035"},{"key":"161","doi-asserted-by":"crossref","unstructured":"161. G\u00f3mez-Fern\u00e1ndez J, G\u00f3mez-Izquierdo E, Tom\u00e1s C, Gonz\u00e1lez-Bulnes A, S\u00e1nchez-S\u00e1nchez R, de Mercado E. Inclusion of seminal plasma in sperm cryopreservation of Iberian pig. Anim Reprod Sci<\/i> 2012; 130: 82\u201390.","DOI":"10.1016\/j.anireprosci.2011.12.017"},{"key":"162","doi-asserted-by":"crossref","unstructured":"162. Rodriguez-Martinez H, Martinez EA, Calvete JJ, Pe\u00f1a Vega FJ, Roca J. Seminal plasma: relevant for fertility? Int J Mol Sci<\/i> 2021; 22: 4368.","DOI":"10.3390\/ijms22094368"},{"key":"163","doi-asserted-by":"crossref","unstructured":"163. Parrilla I, Perez-Pati\u00f1o C, Li J, Barranco I, Padilla L, Rodriguez-Martinez H, Martinez EA, Roca J. Boar semen proteomics and sperm preservation. Theriogenology<\/i> 2019; 137: 23\u201329.","DOI":"10.1016\/j.theriogenology.2019.05.033"},{"key":"164","doi-asserted-by":"crossref","unstructured":"164. Blanch E, Tom\u00e1s C, Graham JK, Moc\u00e9 E. Response of boar sperm to the treatment with cholesterol-loaded cyclodextrins added prior to cryopreservation. Reprod Domest Anim<\/i> 2012; 47: 959\u2013964.","DOI":"10.1111\/j.1439-0531.2012.01999.x"},{"key":"165","doi-asserted-by":"crossref","unstructured":"165. Tom\u00e1s C, Blanch E, Hern\u00e1ndez M, Gil MA, Roca J, V\u00e1zquez JM, Mart\u00ednez EA, Moc\u00e9 E. Treating boar sperm with cholesterol-loaded cyclodextrins widens the sperm osmotic tolerance limits and enhances the in vitro sperm fertilising ability. Anim Reprod Sci<\/i> 2011; 129: 209\u2013220.","DOI":"10.1016\/j.anireprosci.2011.12.003"},{"key":"166","doi-asserted-by":"crossref","unstructured":"166. Tom\u00e1s C, Blanch E, Fazeli A, Moc\u00e9 E. Effect of a pre-freezing treatment with cholesterol-loaded cyclodextrins on boar sperm longevity, capacitation dynamics, ability to adhere to porcine oviductal epithelial cells in vitro and DNA fragmentation dynamics. Reprod Fertil Dev<\/i> 2013; 25: 935\u2013946.","DOI":"10.1071\/RD12079"},{"key":"167","doi-asserted-by":"crossref","unstructured":"167. Wang J, Rao Y, Du C, Wang A, Wu Y, Lin R, Xiao T, Lin W. Sodium alginate and bovine serum albumin co-combined improved the cryopreservation quality of boar sperm through the PI3K-AKT pathway. Reprod Fertil Dev<\/i> 2025; 37: RD24127.","DOI":"10.1071\/RD24127"},{"key":"168","doi-asserted-by":"crossref","unstructured":"168. Masuda Y, Kheawkanha T, Nagahama A, Kawasaki K, Konno T, Yamanaka K, Tatemoto H. Antifreeze protein type III addition to freezing extender comprehensively improves post-thaw sperm properties in Okinawan native Agu pig. Anim Reprod Sci<\/i> 2023; 252: 107232.","DOI":"10.1016\/j.anireprosci.2023.107232"},{"key":"169","doi-asserted-by":"crossref","unstructured":"169. Kaeoket K, Chanapiwat P, Tummaruk P, Techakumphu M. Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen. Asian J Androl<\/i> 2010; 12: 760\u2013765.","DOI":"10.1038\/aja.2010.48"},{"key":"170","doi-asserted-by":"crossref","unstructured":"170. Liu C, Feng H, Han J, Zhou H, Yuan L, Pan H, Wang X, Han X, Qiao R, Yang F, Li X. Effect of L-proline on sperm quality during cryopreservation of boar semen. Anim Reprod Sci<\/i> 2023; 258: 107359.","DOI":"10.1016\/j.anireprosci.2023.107359"},{"key":"171","doi-asserted-by":"crossref","unstructured":"171. Jin H, Choi W, Matsumura K, Hyon S-H, Gen Y, Hayashi M, Kawabata T, Ijiri M, Miyoshi K. Cryopreservation of pig spermatozoa using carboxylated poly-L-lysine as cryoprotectant. J Reprod Dev<\/i> 2022; 68: 312\u2013317.","DOI":"10.1262\/jrd.2022-058"},{"key":"172","doi-asserted-by":"crossref","unstructured":"172. Chanapiwat P, Kaeoket K, Tummaruk P. Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen. Asian J Androl<\/i> 2009; 11: 600\u2013608.","DOI":"10.1038\/aja.2009.40"},{"key":"173","doi-asserted-by":"crossref","unstructured":"173. Jeong YJ, Kim MK, Song HJ, Kang EJ, Ock SA, Kumar BM, Balasubramanian S, Rho GJ. Effect of \u03b1-tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes. Cryobiology<\/i> 2009; 58: 181\u2013189.","DOI":"10.1016\/j.cryobiol.2008.12.004"},{"key":"174","doi-asserted-by":"crossref","unstructured":"174. Satorre MM, Breininger E, Beconi MT. Cryopreservation with \u03b1-tocopherol and Sephadex filtration improved the quality of boar sperm. Theriogenology<\/i> 2012; 78: 1548\u20131556.","DOI":"10.1016\/j.theriogenology.2012.06.023"},{"key":"175","doi-asserted-by":"crossref","unstructured":"175. Roca J, Gil MA, Hernandez M, Parrilla I, Vazquez JM, Martinez EA. Survival and fertility of boar spermatozoa after freeze-thawing in extender supplemented with butylated hydroxytoluene. J Androl<\/i> 2004; 25: 397\u2013405.","DOI":"10.1002\/j.1939-4640.2004.tb02806.x"},{"key":"176","doi-asserted-by":"crossref","unstructured":"176. Giaretta E, Estrada E, Bucci D, Spinaci M, Rodr\u00edguez-Gil JE, Yeste M. Combining reduced glutathione and ascorbic acid has supplementary beneficial effects on boar sperm cryotolerance. Theriogenology<\/i> 2015; 83: 399\u2013407.","DOI":"10.1016\/j.theriogenology.2014.10.002"},{"key":"177","doi-asserted-by":"crossref","unstructured":"177. Estrada E, Rivera Del \u00c1lamo MM, Rodr\u00edguez-Gil JE, Yeste M. The addition of reduced glutathione to cryopreservation media induces changes in the structure of motile subpopulations of frozen-thawed boar sperm. Cryobiology<\/i> 2017; 78: 56\u201364.","DOI":"10.1016\/j.cryobiol.2017.07.002"},{"key":"178","doi-asserted-by":"crossref","unstructured":"178. de Andrade AFC, Balogun K, Machaty Z, Knox RV. Effects of supplemental antioxidants on in\u00a0vitro fertility measures for cryopreserved boar spermatozoa. Theriogenology<\/i> 2023; 200: 33\u201342.","DOI":"10.1016\/j.theriogenology.2023.01.025"},{"key":"179","doi-asserted-by":"crossref","unstructured":"179. Li J, Wang H, Guo M, Li T, Zhang H, Zhang Q, Wang Q, Song Y, Feng H, Wei G. Exogenous spermidine effectively improves the quality of cryopreserved boar sperm. Anim Sci J<\/i> 2023; 94: e13859.","DOI":"10.1111\/asj.13859"},{"key":"180","doi-asserted-by":"crossref","unstructured":"180. Wang H, Guo M, Li T, Zhang H, Wang Q, Zhang Q, Song Y, Feng H, Li Y, Wei G, Li J. Phosphocreatine addition to extender enhances the quality and antioxidant capacity of cryopreserved boar sperm. Reprod Domest Anim<\/i> 2023; 58: 1087\u20131096.","DOI":"10.1111\/rda.14404"},{"key":"181","doi-asserted-by":"crossref","unstructured":"181. Shi L, Zhang Y, Huang X, Shi M, Sun D, Zhang Y, Li W, Jin T, Feng J, Xing J, Li B, Cao G. Effects of mitoquinone (MitoQ) supplementation during boar semen cryopreservation on sperm quality, antioxidant status and mitochondrial proteomics. Anim Reprod Sci<\/i> 2022; 247: 107099.","DOI":"10.1016\/j.anireprosci.2022.107099"},{"key":"182","doi-asserted-by":"crossref","unstructured":"182. Almubarak A, Lee S, Yu I-J, Jeon Y. Effects of Nobiletin supplementation on the freezing diluent on porcine sperm cryo-survival and subsequent in vitro embryo development. Theriogenology<\/i> 2024; 214: 314\u2013322.","DOI":"10.1016\/j.theriogenology.2023.11.006"},{"key":"183","doi-asserted-by":"crossref","unstructured":"183. Ma S, Liang Q, Li Z, He F, Wang Y, Zhuang Z, Zhou J, Zhao Y, Chen F. The cryoprotective effect of virgin coconut oil on boar sperm: Enhancing sperm quality and reproductive performance. Anim Reprod Sci<\/i> 2025; 282: 107998.","DOI":"10.1016\/j.anireprosci.2025.107998"},{"key":"184","doi-asserted-by":"crossref","unstructured":"184. Zhang G, Wen F, Li Y, Sun P, Li Y, Hu Z, Wang H, Ma Y, Liang G, Chen L, Yang K, Hu J. Sulforaphane acts through the NFE2L2\/AMPK signaling pathway to protect boar spermatozoa from cryoinjury by activating antioxidant defenses. Theriogenology<\/i> 2024; 230: 330\u2013340.","DOI":"10.1016\/j.theriogenology.2024.09.030"},{"key":"185","doi-asserted-by":"crossref","unstructured":"185. Mart\u00edn-San Juan A, de Mercado E, Nieto-Crist\u00f3bal H, Cabero A, Silvestre M\u00c1, Morrell JM, \u00c1lvarez-Rodr\u00edguez M. Protective effect of supplementation with water soluble \u03b2-carotene and \u03b1-tocopherol in boar sperm cooling-freezing extender, but not in the thawing extender. Anim Reprod Sci<\/i> 2025; 274: 107792.","DOI":"10.1016\/j.anireprosci.2025.107792"},{"key":"186","doi-asserted-by":"crossref","unstructured":"186. Balogun KB, Nicholls G, Sokunbi OA, Stewart KR. Cryoprotectant effects of natural honey on spermatozoa quality of pre-freezing and frozen-thawed boar semen. J Anim Sci<\/i> 2023; 101: skac384.","DOI":"10.1093\/jas\/skac384"},{"key":"187","doi-asserted-by":"crossref","unstructured":"187. Kalita MK, Baruah PM, Ahmed K, Sinha S, Tamuly S, Deori S, Katiyar R, Abedin SN, Patowary P, Dewry R, Das J, Sarkar B, Khargharia G, Kadirvel G. Supplemental effect of metallic nanoparticles on cryopreserved semen quality, lipid peroxidation, sperm head ultrastructure and field fertility levels in crossbred (Hampshire x Niang Megha) boars. Cryobiology<\/i> 2025; 120: 105277.","DOI":"10.1016\/j.cryobiol.2025.105277"},{"key":"188","doi-asserted-by":"crossref","unstructured":"188. \u00c1lvarez-Rodr\u00edguez M, Nieto-Cristobal H, de Mercado E. Bovine serum albumin inclusion in the thawing extender improves boar sperm membrane and acrosomal integrity. Reprod Domest Anim<\/i> 2024; 59(Suppl 3): e14592.","DOI":"10.1111\/rda.14592"},{"key":"189","doi-asserted-by":"crossref","unstructured":"189. Zhang H, Wang S, Wang Q, Min L, Bastaw EM, Zhu Z. Long-chain fatty acids promote ATP production in post-thaw boar sperm through mitochondrial \u03b2-oxidation. Anim Reprod Sci<\/i> 2025; 281: 107985.","DOI":"10.1016\/j.anireprosci.2025.107985"},{"key":"190","doi-asserted-by":"crossref","unstructured":"190. Johannisson A, Morrell JM, Wallgren M. Enrichment of thawed boar spermatozoa with an intact membrane using Magnetic Activated Cell Sorting. Anim Reprod Sci<\/i> 2024; 265: 107493.","DOI":"10.1016\/j.anireprosci.2024.107493"},{"key":"191","doi-asserted-by":"crossref","unstructured":"191. Nguyen ST, Taniguchi M, Ono T, Takagi M, Lin Q, Torigoe N, Liu B, Namula Z, Nagahara M, Otoi T. Quality and fertilizing ability of frozen-thawed porcine sperm separated using a migration sedimentation method. Reprod Domest Anim<\/i> 2024; 59: e14648.","DOI":"10.1111\/rda.14648"},{"key":"192","doi-asserted-by":"crossref","unstructured":"192. Li J, Wang H, Guo M, Guo Q, Li Y. Combination of exogenous spermidine and phosphocreatine efficiently improved the quality and antioxidant capacity of cryopreserved boar sperm and reduced apoptosis-like changes. Mol Reprod Dev<\/i> 2024; 91: e70003.","DOI":"10.1002\/mrd.70003"}],"container-title":["Journal of Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/3\/72_2026-005\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T03:26:34Z","timestamp":1781925994000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/3\/72_2026-005\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":192,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.1262\/jrd.2026-005","relation":{},"ISSN":["0916-8818","1348-4400"],"issn-type":[{"value":"0916-8818","type":"print"},{"value":"1348-4400","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"2026-005"}}