{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T04:58:51Z","timestamp":1785560331086,"version":"3.56.0"},"reference-count":17,"publisher":"Japanese Society of Animal Reproduction","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Reprod. Dev.","Journal of Reproduction and Development"],"published-print":{"date-parts":[[2026]]},"DOI":"10.1262\/jrd.2026-014","type":"journal-article","created":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T22:08:59Z","timestamp":1774217339000},"page":"719-722","source":"Crossref","is-referenced-by-count":0,"title":["Sex determination and genetic screening of equine embryos with whole genome amplification and real-time PCR"],"prefix":"10.1262","volume":"72","author":[{"given":"Gus R.","family":"MCFARLANE","sequence":"first","affiliation":[{"name":"NSW Department of Primary Industries and Regional Development, Elizabeth Macarthur Agricultural Institute, Menangle, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"BACKHOUSE","sequence":"additional","affiliation":[{"name":"Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agustin","family":"RUIZ","sequence":"additional","affiliation":[{"name":"Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendon A.","family":"O\u2019ROURKE","sequence":"additional","affiliation":[{"name":"NSW Department of Primary Industries and Regional Development, Elizabeth Macarthur Agricultural Institute, Menangle, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenin V.","family":"CORTEZ","sequence":"additional","affiliation":[{"name":"Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW, Australia"},{"name":"Catalina Genetics Pty Ltd, North Richmond, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher G.","family":"GRUPEN","sequence":"additional","affiliation":[{"name":"Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"349","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1. De Coster T, Zhao Y, T\u0161uiko O, Demyda-Peyr\u00e1s S, Van Soom A, Vermeesch JR, Smits K. Genome-wide equine preimplantation genetic testing enabled by simultaneous haplotyping and copy number detection. Sci Rep<\/i> 2024; 14: 2003.","DOI":"10.1038\/s41598-023-48103-7"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Barandalla M, Colleoni S, Perota A, Galli C, Lazzari G. Preimplantation genetic testing in horses: biopsy of Piezo-ICSI embryos for sex, coat color, and disease alleles. Theriogenology<\/i> 2025; 246: 117525.","DOI":"10.1016\/j.theriogenology.2025.117525"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. Ram\u00edrez-Ag\u00e1mez L, Castaneda C, Hern\u00e1ndez-Avil\u00e9s C, Grahn RA, Raudsepp T, Love CC. A study on methods for preimplantation genetic testing (PGT) on in vivo- and in vitro-produced equine embryos, with emphasis on embryonic sex determination. Theriogenology<\/i> 2024; 227: 41\u201348.","DOI":"10.1016\/j.theriogenology.2024.07.009"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. De Coster T, Van Poucke M, Bogado Pascottini O, Angel-Velez D, Van den Branden E, Peere S, Papas M, Gerits I, Govaere J, Peelman L, Vermeesch JR, Van Soom A, Smits K. Single closed-tube quantitative real-time PCR assay with dual-labelled probes for improved sex determination of equine embryos. Animal<\/i> 2023; 17: 100952.","DOI":"10.1016\/j.animal.2023.100952"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Resende HL, Carmo MT, Ramires Neto C, Alvarenga MA. Determination of equine fetal sex by Doppler ultrasonography of the gonads. Equine Vet J<\/i> 2014; 46: 756\u2013758.","DOI":"10.1111\/evj.12213"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Wood TC, White KL, Thompson DL Jr, Garza F Jr. Evaluation of the expression of a male-specific antigen on cells of equine blastocysts. J Reprod Immunol<\/i> 1988; 14: 1\u20138.","DOI":"10.1016\/0165-0378(88)90031-9"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Riera FL, Rold\u00e1n JE, Espinosa JM, Fernandez JE, Ortiz I, Hinrichs K. Application of embryo biopsy and sex determination via polymerase chain reaction in a commercial equine embryo transfer program in Argentina. Reprod Fertil Dev<\/i> 2019; 31: 1917\u20131925.","DOI":"10.1071\/RD19228"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Gibb Z, Grupen CG, Maxwell WM, Morris LH. Field fertility of liquid stored and cryopreserved flow cytometrically sex-sorted stallion sperm. Equine Vet J<\/i> 2017; 49: 160\u2013166.","DOI":"10.1111\/evj.12583"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Panarace M, Pellegrini RO, Basualdo MO, Bel\u00e9 M, Ursino DA, Cisterna R, Desimone G, Rodr\u00edguez E, Medina MJ. First field results on the use of stallion sex-sorted semen in a large-scale embryo transfer program. Theriogenology<\/i> 2014; 81: 520\u2013525.","DOI":"10.1016\/j.theriogenology.2013.10.021"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Valberg SJ. Genetics of equine muscle disease. Vet Clin North Am Equine Pract<\/i> 2020; 36: 353\u2013378.","DOI":"10.1016\/j.cveq.2020.03.012"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Grady JG, Elder SH, Ryan PL, Swiderski CE, Rashmir-Raven AM. Biomechanical and molecular characteristics of hereditary equine regional dermal asthenia in Quarter Horses. Vet Dermatol<\/i> 2009; 20: 591\u2013599.","DOI":"10.1111\/j.1365-3164.2009.00830.x"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Cortez JV, Hardwicke K, Cuervo-Arango J, Grupen CG. Cloning horses by somatic cell nuclear transfer: Effects of oocyte source on development to foaling. Theriogenology<\/i> 2023; 203: 99\u2013108.","DOI":"10.1016\/j.theriogenology.2023.03.018"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Lazzari G, Colleoni S, Crotti G, Turini P, Fiorini G, Barandalla M, Landriscina L, Dolci G, Benedetti M, Duchi R, Galli C. Laboratory production of equine embryos. J Equine Vet Sci<\/i> 2020; 89: 103097.","DOI":"10.1016\/j.jevs.2020.103097"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Thompson JGE, Simpson AC, Pugh PA, Donnelly PE, Tervit HR. Effect of oxygen concentration on in-vitro development of preimplantation sheep and cattle embryos. J Reprod Fertil<\/i> 1990; 89: 573\u2013578.","DOI":"10.1530\/jrf.0.0890573"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Cortez JV, Hardwicke K, M\u00e9ndez-Calder\u00f3n CE, Grupen CG. Effect of pre-IVM duration with cAMP modulators on the production of cloned equine embryos and foals. Animals (Basel)<\/i> 2025; 15: 1961. .","DOI":"10.3390\/ani15131961"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. Gibb Z, Morris LH, Maxwell WM, Grupen CG. Dimethyl formamide improves the postthaw characteristics of sex-sorted and nonsorted stallion sperm. Theriogenology<\/i> 2013; 79: 1027\u20131033.","DOI":"10.1016\/j.theriogenology.2013.01.013"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. Felix MR, Turner RM, Dobbie T, Hinrichs K. Successful in vitro fertilization in the horse: production of blastocysts and birth of foals after prolonged sperm incubation for capacitation\u2020. Biol Reprod<\/i> 2022; 107: 1551\u20131564.","DOI":"10.1093\/biolre\/ioac172"}],"container-title":["Journal of Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/4\/72_2026-014\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T04:23:56Z","timestamp":1785558236000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/4\/72_2026-014\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.1262\/jrd.2026-014","relation":{},"ISSN":["0916-8818","1348-4400"],"issn-type":[{"value":"0916-8818","type":"print"},{"value":"1348-4400","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"2026-014"}}