{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T03:52:31Z","timestamp":1781927551265,"version":"3.54.5"},"reference-count":124,"publisher":"Japanese Society of Animal Reproduction","issue":"3","funder":[{"DOI":"10.13039\/100000865","name":"Bill & Melinda Gates Foundation","doi-asserted-by":"crossref","award":["INV-056260"],"award-info":[{"award-number":["INV-056260"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"crossref"}]},{"name":"JSPS KAKENHI","award":["22HP2009"],"award-info":[{"award-number":["22HP2009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Reprod. Dev.","Journal of Reproduction and Development"],"published-print":{"date-parts":[[2026]]},"DOI":"10.1262\/jrd.2026-021","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T22:11:03Z","timestamp":1781734263000},"page":"330-337","source":"Crossref","is-referenced-by-count":0,"title":["Towards cervical AI of sheep with stored semen: latest insights and future developments"],"prefix":"10.1262","volume":"72","author":[{"given":"Elie","family":"BARAKAT","sequence":"first","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, PRC, Nouzilly, F-37380, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo","family":"PRUDHOMME","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, PRC, Nouzilly, F-37380, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"ADRIAENSEN","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, PRC, Nouzilly, F-37380, France"},{"name":"INRAE, PIXANIM, Centre de Recherche de Tours, Nouzilly, F-37380, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Emilie","family":"LEBACHELIER DE LA RIVI\u00c8RE","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, PRC, Nouzilly, F-37380, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"BARBEY","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, PRC, Nouzilly, F-37380, France"},{"name":"INRAE, PIXANIM, Centre de Recherche de Tours, Nouzilly, F-37380, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"REYNAUD","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, PRC, Nouzilly, F-37380, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"TSIKIS","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, PRC, Nouzilly, F-37380, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"LASSERRE","sequence":"additional","affiliation":[{"name":"INRAE, UEPAO, Centre de Recherche de Tours, Nouzilly, F-37380, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon P.","family":"DE GRAAF","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"DRUART","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, PRC, Nouzilly, F-37380, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"349","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1. Gibbons AE, Fernandez J, Bruno-Galarraga MM, Spinelli MV, Cueto MI. Technical recommendations for artificial insemination in sheep. Anim Reprod<\/i> 2019; 16: 803\u2013809.","DOI":"10.21451\/1984-3143-AR2018-0129"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Raoul J, Elsen J-M. Effect of the rate of artificial insemination and paternity knowledge on the genetic gain for French meat sheep breeding programs. Livest Sci<\/i> 2020; 232: 103932.","DOI":"10.1016\/j.livsci.2020.103932"},{"key":"3","unstructured":"3. Gillan L, Maxwell WM. The functional integrity and fate of cryopreserved ram spermatozoa in the female tract. J Reprod Fertil Suppl<\/i> 1999; 54: 271\u2013283."},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Rickard JP, Pool KR, Druart X, de Graaf SP. The fate of spermatozoa in the female reproductive tract: A\u00a0comparative review. Theriogenology<\/i> 2019; 137: 104\u2013112.","DOI":"10.1016\/j.theriogenology.2019.05.044"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Fair S, Meade KG, Reynaud K, Druart X, de Graaf SP. The biological mechanisms regulating sperm selection by the ovine cervix. Reproduction<\/i> 2019; 158: R1\u2013R13.","DOI":"10.1530\/REP-18-0595"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Spanner EA, de Graaf SP, Rickard JP. Factors affecting the success of laparoscopic artificial insemination in sheep. Anim Reprod Sci<\/i> 2024; 264: 107453.","DOI":"10.1016\/j.anireprosci.2024.107453"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Spanner EA, de Graaf SP, Rickard JP. A multivariate model for the prediction of pregnancy following laparoscopic artificial insemination of sheep. Sci Rep<\/i> 2024; 14: 27556.","DOI":"10.1038\/s41598-024-79253-x"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. El Amiri B, Rahim A. Exploring endogenous and exogenous factors for successful artificial insemination in sheep: a global overview. Vet Sci<\/i> 2024; 11: 86.","DOI":"10.3390\/vetsci11020086"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Mikkola M, Desmet KLJ, Kommisrud E, Riegler MA. Recent advancements to increase success in assisted reproductive technologies in cattle. Anim Reprod<\/i> 2024; 21: e20240031.","DOI":"10.1590\/1984-3143-ar2024-0031"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Leboeuf B, Restall B, Salamon S. Production and storage of goat semen for artificial insemination. Anim Reprod Sci<\/i> 2000; 62: 113\u2013141.","DOI":"10.1016\/S0378-4320(00)00156-1"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Y\u00e1nez-Ortiz I, Catal\u00e1n J, Rodr\u00edguez-Gil JE, Mir\u00f3 J, Yeste M. Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep. Anim Reprod Sci<\/i> 2022; 246: 106904.","DOI":"10.1016\/j.anireprosci.2021.106904"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Pini T, Leahy T, de Graaf SP. Sublethal sperm freezing damage: Manifestations and solutions. Theriogenology<\/i> 2018; 118: 172\u2013181.","DOI":"10.1016\/j.theriogenology.2018.06.006"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Sun W, Jiang S, Su J, Zhang J, Bao X, Ding R, Shi P, Li S, Wu C, Zhao G, Cao G, Sun QY, Yu H, Li X. The effects of cryopreservation on the acrosome structure, enzyme activity, motility, and fertility of bovine, ovine, and goat sperm. Anim Reprod<\/i> 2021; 17: e20200219.","DOI":"10.1590\/1984-3143-ar2020-0219"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Khalil WA, Sharaf AE, Khalifa EI, El-Harairy MA, Swelum AA, Abdelnour SA. Recent approaches in the use of antioxidants and proteomic modifications in ram semen preservation. Reprod Domest Anim<\/i> 2023; 58: 1639\u20131653.","DOI":"10.1111\/rda.14485"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Allai L, Benmoula A, Marciane da Silva M, Nasser B, El Amiri B. Supplementation of ram semen extender to improve seminal quality and fertility rate. Anim Reprod Sci<\/i> 2018; 192: 6\u201317.","DOI":"10.1016\/j.anireprosci.2018.03.019"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. Jia B, Larbi A, Lv C, Liang J, Xiang D, Zhang B, Fang Y, Shen W, Wu G, Quan G. Identification and validation of ram sperm proteins associated with cryoinjuries caused by the cryopreservation process. Theriogenology<\/i> 2022; 184: 191\u2013203.","DOI":"10.1016\/j.theriogenology.2022.03.015"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. He Y, Wang K, Zhao X, Zhang Y, Ma Y, Hu J. Differential proteome association study of freeze-thaw damage in ram sperm. Cryobiology<\/i> 2016; 72: 60\u201368.","DOI":"10.1016\/j.cryobiol.2015.11.003"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18. Lv C, Larbi A, Memon S, Liang J, Zhao X, Shao Q, Wu G, Quan G. The proteomic characterization of ram sperm during cryopreservation analyzed by the two-dimensional electrophoresis coupled with mass spectrometry. Cryobiology<\/i> 2020; 97: 37\u201345.","DOI":"10.1016\/j.cryobiol.2020.10.011"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19. Zang S, Yang X, Ye J, Mo X, Zhou G, Fang Y. Quantitative phosphoproteomics explain cryopreservation-induced reductions in ram sperm motility. J Proteomics<\/i> 2024; 298: 105153.","DOI":"10.1016\/j.jprot.2024.105153"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20. Ren C, Sun Z, Chen Y, Chen J, Wang S, Liu Q, Wang P, Cheng X, Zhang Z, Wang Q. Identification of biomarkers affecting cryopreservation recovery ratio in ram spermatozoa using tandem mass tags (TMT)-based quantitative proteomics approach. Animals (Basel)<\/i> 2023; 13: 2368.","DOI":"10.3390\/ani13142368"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21. Ofosu J, Nartey MA, Mo X, Ye J, Zhang Y, Zeng C, Zhang M, Fang Y, Zhou G. Ram sperm cryopreservation disrupts metabolism of unsaturated fatty acids. Theriogenology<\/i> 2023; 204: 8\u201317.","DOI":"10.1016\/j.theriogenology.2023.03.023"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22. Colas G. Effect of initial freezing temperature, addition of glycerol and dilution on the survival and fertilizing ability of deep-frozen ram semen. J Reprod Fertil<\/i> 1975; 42: 277\u2013285.","DOI":"10.1530\/jrf.0.0420277"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23. Saha A, Asaduzzaman M, Bari FY. Cryopreservation techniques for ram sperm. Vet Med Int<\/i> 2022; 2022: 7378379.","DOI":"10.1155\/2022\/7378379"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24. Marco-Jim\u00e9nez F, Puchades S, Moc\u00e9 E, Viudes-de-Cartro MP, Vicente JS, Rodriguez M. Use of powdered egg yolk vs fresh egg yolk for the cryopreservation of ovine semen. Reprod Domest Anim<\/i> 2004; 39: 438\u2013441.","DOI":"10.1111\/j.1439-0531.2004.00537.x"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25. Kulaks\u0131z R, \u00c7ebi \u00c7, Ak\u00e7ay E, Da\u015fk\u0131n A. The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Rumin Res<\/i> 2010; 88: 12\u201315.","DOI":"10.1016\/j.smallrumres.2009.11.014"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26. Carro MLM, Pe\u00f1alva DA, Antollini SS, Hozbor FA, Buschiazzo J. Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality. Biochim Biophys Acta Biomembr<\/i> 2020; 1862: 183357.","DOI":"10.1016\/j.bbamem.2020.183357"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27. Carro MLM, Ram\u00edrez-Vasquez RRA, Pe\u00f1alva DA, Buschiazzo J, Hozbor FA. Desmosterol incorporation into ram sperm membrane before cryopreservtion improves in vitro and in vivo fertility. Front Cell Dev Biol<\/i> 2021; 9: 660165.","DOI":"10.3389\/fcell.2021.660165"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28. Pini T, Rickard JP, Leahy T, Crossett B, Druart X, de Graaf SP. Cryopreservation and egg yolk medium alter the proteome of ram spermatozoa. J Proteomics<\/i> 2018; 181: 73\u201382.","DOI":"10.1016\/j.jprot.2018.04.001"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29. Maxwell WM, de Graaf SP, Ghaoui R-H, Evans G. Seminal plasma effects on sperm handling and female fertility. Soc Reprod Fertil Suppl<\/i> 2007; 64: 13\u201338.","DOI":"10.5661\/RDR-VI-13"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30. Druart X, de Graaf S. Seminal plasma proteomes and sperm fertility. Anim Reprod Sci<\/i> 2018; 194: 33\u201340.","DOI":"10.1016\/j.anireprosci.2018.04.061"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31. Ben moula A, El Amiri B. Factors influencing seminal plasma composition and its relevance to succeed sperm technology in sheep: An updated review. Small Rumin Res<\/i> 2022; 215: 106759.","DOI":"10.1016\/j.smallrumres.2022.106759"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32. Leahy T, Rickard JP, Bernecic NC, Druart X, de Graaf SP. Ram seminal plasma and its functional proteomic assessment. Reproduction<\/i> 2019; 157: R243\u2013R256.","DOI":"10.1530\/REP-18-0627"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33. Mart\u00ednez-Fresneda L, Casta\u00f1o C, B\u00f3veda P, Tesfaye D, Schellander K, Santiago-Moreno J, Garc\u00eda-V\u00e1zquez FA. Epididymal and ejaculated sperm differ on their response to the cryopreservation and capacitation processes in mouflon (Ovis musimon). Sci Rep<\/i> 2019; 9: 15659.","DOI":"10.1038\/s41598-019-52057-0"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34. Tsikis G, Reynaud K, Ferchaud S, Druart X. Seminal plasma differentially alters the resistance of dog, ram and boar spermatozoa to hypotonic stress. Anim Reprod Sci<\/i> 2018; 193: 1\u20138.","DOI":"10.1016\/j.anireprosci.2018.01.012"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35. Pini T, Leahy T, Soleilhavoup C, Tsikis G, Labas V, Combes-Soia L, Harichaux G, Rickard JP, Druart X, de Graaf SP. Proteomic investigation of ram spermatozoa and the proteins conferred by seminal plasma. J Proteome Res<\/i> 2016; 15: 3700\u20133711.","DOI":"10.1021\/acs.jproteome.6b00530"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36. van Tilburg M, Sousa S, Lobo MDP, Monteiro-Azevedo ACOM, Azevedo RA, Ara\u00fajo AA, Moura AA. Mapping the major proteome of reproductive fluids and sperm membranes of rams: From the cauda epididymis to ejaculation. Theriogenology<\/i> 2021; 159: 98\u2013107.","DOI":"10.1016\/j.theriogenology.2020.10.003"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37. van Tilburg MF, Rodrigues MAM, Moreira RA, Moreno FB, Monteiro-Moreira ACO, C\u00e2ndido MJD, Moura AA. Membrane-associated proteins of ejaculated sperm from Morada Nova rams. Theriogenology<\/i> 2013; 79: 1247\u20131261.","DOI":"10.1016\/j.theriogenology.2013.03.013"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38. Leahy T, Rickard JP, Pini T, Gadella BM, de Graaf SP. Quantitative proteomic analysis of seminal plasma, sperm membrane proteins, and seminal extracellular vesicles suggests vesicular mechanisms aid in the removal and addition of proteins to the ram sperm membrane. Proteomics<\/i> 2020; 20: e1900289.","DOI":"10.1002\/pmic.201900289"},{"key":"39","doi-asserted-by":"crossref","unstructured":"39. Leahy T, de Graaf SP. Seminal plasma and its effect on ruminant spermatozoa during processing. Reprod Domest Anim<\/i> 2012; 47(Suppl 4): 207\u2013213.","DOI":"10.1111\/j.1439-0531.2012.02077.x"},{"key":"40","doi-asserted-by":"crossref","unstructured":"40. Pini T, de Graaf SP, Druart X, Tsikis G, Labas V, Teixeira-Gomes AP, Gadella BM, Leahy T. Binder of Sperm Proteins 1 and 5 have contrasting effects on the capacitation of ram spermatozoa. Biol Reprod<\/i> 2018; 98: 765\u2013775.","DOI":"10.1093\/biolre\/ioy032"},{"key":"41","doi-asserted-by":"crossref","unstructured":"41. Rodrigues MAM, Souza CEA, Martins JAM, Rego JPA, Oliveira JTA, Domont G, Nogueira FCS, Moura AA. Seminal plasma proteins and their relationship with sperm motility in Santa Ines rams. Small Rumin Res<\/i> 2013; 109: 94\u2013100.","DOI":"10.1016\/j.smallrumres.2012.07.032"},{"key":"42","doi-asserted-by":"crossref","unstructured":"42. Pini T, Farmer K, Druart X, Teixeira-Gomes AP, Tsikis G, Labas V, Leahy T, de Graaf SP. Binder of Sperm Proteins protect ram spermatozoa from freeze-thaw damage. Cryobiology<\/i> 2018; 82: 78\u201387.","DOI":"10.1016\/j.cryobiol.2018.04.005"},{"key":"43","doi-asserted-by":"crossref","unstructured":"43. \u00c1lvarez M, Tamayo-Canul J, Mart\u00ednez-Rodr\u00edguez C, L\u00f3pez-Urue\u00f1a E, Gomes-Alves S, Anel L, Mart\u00ednez-Pastor F, de Paz P. Specificity of the extender used for freezing ram sperm depends of the spermatozoa source (ejaculate, electroejaculate or epididymis). Anim Reprod Sci<\/i> 2012; 132: 145\u2013154.","DOI":"10.1016\/j.anireprosci.2012.05.006"},{"key":"44","doi-asserted-by":"crossref","unstructured":"44. Rickard JP, Pini T, Soleilhavoup C, Cogni\u00e9 J, Bathgate R, Lynch GW, Evans G, Maxwell WMC, Druart X, de Graaf SP. Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa. Reproduction<\/i> 2014; 148: 469\u2013478.","DOI":"10.1530\/REP-14-0285"},{"key":"45","doi-asserted-by":"crossref","unstructured":"45. Scott JL, Ketheesan N, Summers PM. Leucocyte population changes in the reproductive tract of the ewe in response to insemination. Reprod Fertil Dev<\/i> 2006; 18: 627\u2013634.","DOI":"10.1071\/RD05165"},{"key":"46","doi-asserted-by":"crossref","unstructured":"46. Scott JL, Ketheesan N, Summers PM. Spermatozoa and seminal plasma induce a greater inflammatory response in the ovine uterus at oestrus than dioestrus. Reprod Fertil Dev<\/i> 2009; 21: 817\u2013826.","DOI":"10.1071\/RD09012"},{"key":"47","doi-asserted-by":"crossref","unstructured":"47. Pini T, Leahy T, Paul de Graaf S. Seminal plasma and cryopreservation alter ram sperm surface carbohydrates and interactions with neutrophils. Reprod Fertil Dev<\/i> 2018; 30: 689\u2013702.","DOI":"10.1071\/RD17251"},{"key":"48","doi-asserted-by":"crossref","unstructured":"48. Warr S, Pini T, de Graaf SP, Rickard JP. Seminal plasma protects frozen-thawed ram spermatozoa from neutrophil attack: a complement mediated dynamic. Reproduction<\/i> 2025; 170: e240465.","DOI":"10.1530\/REP-24-0465"},{"key":"49","doi-asserted-by":"crossref","unstructured":"49. Mikhael L, Warr S, de Graaf SP, Rickard JP. Evaluating sperm-polymorphonuclear leukocyte interactions in vitro: the immunomodulatory role of seminal plasma and implications for future ovine studies. Reprod Fertil Dev<\/i> 2026; 38: RD25131.","DOI":"10.1071\/RD25131"},{"key":"50","doi-asserted-by":"crossref","unstructured":"50. Rickard JP, Warr S, de Graaf SP, Pini T. Ram sperm and seminal plasma alter immune transcriptome and cytokine secretion in ovine cervical explants ex vivo. Reproduction<\/i> 2025; 170: e250240.","DOI":"10.1530\/REP-25-0240"},{"key":"51","doi-asserted-by":"crossref","unstructured":"51. Ibrahim NM, Troedsson MH, Foster DN, Loseth KJ, Farris JA, Blaschuk O, Crabo BG. Reproductive tract secretions and bull spermatozoa contain different clusterin isoforms that cluster cells and inhibit complement-induced cytolysis. J Androl<\/i> 1999; 20: 230\u2013240.","DOI":"10.1002\/j.1939-4640.1999.tb02514.x"},{"key":"52","doi-asserted-by":"crossref","unstructured":"52. Moura AA, Memili E, Portela AMR, Viana AG, Velho ALC, Bezerra MJB, Vasconselos FR. Seminal plasma proteins and metabolites: effects on sperm function and potential as fertility markers. Anim Reprod<\/i> 2018; 15(Suppl 1): 691\u2013702.","DOI":"10.21451\/1984-3143-AR2018-0029"},{"key":"53","doi-asserted-by":"crossref","unstructured":"53. Rickard JP, Schmidt RE, Maddison JW, Bathgate R, Lynch GW, Druart X, de Graaf SP. Variation in seminal plasma alters the ability of ram spermatozoa to survive cryopreservation. Reprod Fertil Dev<\/i> 2016; 28: 516\u2013523.","DOI":"10.1071\/RD14123"},{"key":"54","doi-asserted-by":"crossref","unstructured":"54. Dayan\u0131kl\u0131 C, B\u00fclb\u00fcl B, Do\u011fan \u015e, \u015eeng\u00fcl E, K\u0131rba\u015f M, Kal Y, Ataman MB. Improving ram semen low cryotolerance by replacing the seminal plasma with that of high-cryotolerant rams or extender. Vet Med Sci<\/i> 2025; 11: e70381.","DOI":"10.1002\/vms3.70381"},{"key":"55","doi-asserted-by":"crossref","unstructured":"55. Rickard JP, Leahy T, Soleilhavoup C, Tsikis G, Labas V, Harichaux G, Lynch GW, Druart X, de Graaf SP. The identification of proteomic markers of sperm freezing resilience in ram seminal plasma. J Proteomics<\/i> 2015; 126: 303\u2013311.","DOI":"10.1016\/j.jprot.2015.05.017"},{"key":"56","doi-asserted-by":"crossref","unstructured":"56. Soleilhavoup C, Tsikis G, Labas V, Harichaux G, Kohnke PL, Dacheux JL, Gu\u00e9rin Y, Gatti JL, de Graaf SP, Druart X. Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa. J Proteomics<\/i> 2014; 109: 245\u2013260.","DOI":"10.1016\/j.jprot.2014.07.007"},{"key":"57","doi-asserted-by":"crossref","unstructured":"57. Maddison JW, Rickard JP, Mooney E, Bernecic NC, Soleilhavoup C, Tsikis G, Druart X, Leahy T, de Graaf SP. Oestrus synchronisation and superovulation alter the production and biochemical constituents of ovine cervicovaginal mucus. Anim Reprod Sci<\/i> 2016; 172: 114\u2013122.","DOI":"10.1016\/j.anireprosci.2016.07.008"},{"key":"58","unstructured":"58. Soleilhavoup C, Riou C, Tsikis G, Kohnke P, Labas V, Harichaux G, G\u00e9rard N, Druart X. Proteomic analysis of fluids from the genital tract of ewes during the oestrous cycle. 26. Colloque Biotechnocentre. Seillac; 2013."},{"key":"59","doi-asserted-by":"crossref","unstructured":"59. Abril-Parre\u00f1o L, Meade KG, Krogen\u00e6s AK, Druart X, Cormican P, Fair S. Ewe breed differences in the cervical transcriptome at the follicular phase of a synchronised oestrous cycle. BMC Genomics<\/i> 2022; 23: 363.","DOI":"10.1186\/s12864-022-08603-8"},{"key":"60","doi-asserted-by":"crossref","unstructured":"60. Donovan A, Hanrahan JP, Kummen E, Duffy P, Boland MP. Fertility in the ewe following cervical insemination with fresh or frozen-thawed semen at a natural or synchronised oestrus. Anim Reprod Sci<\/i> 2004; 84: 359\u2013368.","DOI":"10.1016\/j.anireprosci.2003.12.014"},{"key":"61","doi-asserted-by":"crossref","unstructured":"61. Paulenz H, Adn\u00f8y T, Fossen OH, S\u00f6derquist L, Berg KA. Effect of deposition site and sperm number on the fertility of sheep inseminated with liquid semen. Vet Rec<\/i> 2002; 150: 299\u2013302.","DOI":"10.1136\/vr.150.10.299"},{"key":"62","doi-asserted-by":"crossref","unstructured":"62. Paulenz H, S\u00f6derquist L, Adn\u00f8y T, Nordstoga AB, Andersen Berg K. Effect of vaginal and cervical deposition of semen on the fertility of sheep inseminated with frozen-thawed semen. Vet Rec<\/i> 2005; 156: 372\u2013375.","DOI":"10.1136\/vr.156.12.372"},{"key":"63","doi-asserted-by":"crossref","unstructured":"63. Abril-Parre\u00f1o L, Fair S. Cervical artificial insemination with frozen-thawed semen in sheep; the secret is in the cervix of Norwegian ewe breeds. Biol Reprod<\/i> 2026; 114: 432\u2013441.","DOI":"10.1093\/biolre\/ioaf084"},{"key":"64","doi-asserted-by":"crossref","unstructured":"64. Evans AC, Duffy P, Crosby TF, Hawken PA, Boland MP, Beard AP. Effect of ram exposure at the end of progestagen treatment on estrus synchronisation and fertility during the breeding season in ewes. Anim Reprod Sci<\/i> 2004; 84: 349\u2013358.","DOI":"10.1016\/j.anireprosci.2003.12.013"},{"key":"65","doi-asserted-by":"crossref","unstructured":"65. Ben Salem I, Rouatbi M, Mlika M, Getachew T, Govind A, Haile A, Raggem M, Lahsoumi R, Rekik M. Response of maiden ewes to the \u2018ram effect\u2019 is a robust management practice and a candidate selection trait for enhanced reproductive performance in drylands. Animal<\/i> 2024; 18: 101332.","DOI":"10.1016\/j.animal.2024.101332"},{"key":"66","doi-asserted-by":"crossref","unstructured":"66. Nakafeero A, Hassen A, Lehloenya KC. Investigation of ram effect and eCG usage in progesterone based oestrous synchronization protocols on fertility of ewes following fixed time artificial insemination. Small Rumin Res<\/i> 2020; 183: 106034.","DOI":"10.1016\/j.smallrumres.2019.106034"},{"key":"67","doi-asserted-by":"crossref","unstructured":"67. Alhamada M, Debus N, Bocquier F. An automated method for the evaluation of ram libido in real mating conditions. Animal<\/i> 2017; 11: 2036\u20132044.","DOI":"10.1017\/S1751731117000611"},{"key":"68","doi-asserted-by":"crossref","unstructured":"68. Alhamada M, Debus N, Lurette A, Bocquier F. Validation of automated electronic oestrus detection in sheep as an alternative to visual observation. Small Rumin Res<\/i> 2016; 134: 97\u2013104.","DOI":"10.1016\/j.smallrumres.2015.12.032"},{"key":"69","doi-asserted-by":"crossref","unstructured":"69. Halbert GW, Dobson H, Walton JS, Buckrell BC. The structure of the cervical canal of the ewe. Theriogenology<\/i> 1990; 33: 977\u2013992.","DOI":"10.1016\/0093-691X(90)90060-7"},{"key":"70","doi-asserted-by":"crossref","unstructured":"70. Kershaw CM, Khalid M, McGowan MR, Ingram K, Leethongdee S, Wax G, Scaramuzzi RJ. The anatomy of the sheep cervix and its influence on the transcervical passage of an inseminating pipette into the uterine lumen. Theriogenology<\/i> 2005; 64: 1225\u20131235.","DOI":"10.1016\/j.theriogenology.2005.02.017"},{"key":"71","doi-asserted-by":"crossref","unstructured":"71. Abril-Parre\u00f1o L, Krogen\u00e6s AK, Byrne CJ, Donovan A, Stuen S, Caldas E, Diskin M, Druart X, Fair S. Ewe breed differences in cervical anatomy and cervicovaginal mucus properties: An international study. Theriogenology<\/i> 2021; 160: 18\u201325.","DOI":"10.1016\/j.theriogenology.2020.10.038"},{"key":"72","doi-asserted-by":"crossref","unstructured":"72. Richardson L, Hanrahan JP, Tharmalingam T, Carrington SD, Lonergan P, Evans ACO, Fair S. Cervical mucus sialic acid content determines the ability of frozen-thawed ram sperm to migrate through the cervix. Reproduction<\/i> 2019; 157: 259\u2013271.","DOI":"10.1530\/REP-18-0547"},{"key":"73","doi-asserted-by":"crossref","unstructured":"73. Lacroix G, Gouyer V, Gottrand F, Desseyn JL. The cervicovaginal mucus barrier. Int J Mol Sci<\/i> 2020; 21: 8266.","DOI":"10.3390\/ijms21218266"},{"key":"74","doi-asserted-by":"crossref","unstructured":"74. Mart\u00edn-Maestro A, Abril-Parre\u00f1o L, Soler AJ, Fair S. Media viscosity affects post-thaw ram sperm rheotactic behaviour. Reprod Domest Anim<\/i> 2024; 59(Suppl 3): e14644.","DOI":"10.1111\/rda.14644"},{"key":"75","doi-asserted-by":"crossref","unstructured":"75. Abdel-Ghani MA, El-Sherry T, Mahmoud G, Nagano M. Implications of ram sperm rheotaxis analysed by microfluidics for fertility. Reprod Domest Anim<\/i> 2020; 55: 1541\u20131547.","DOI":"10.1111\/rda.13804"},{"key":"76","doi-asserted-by":"crossref","unstructured":"76. Woolley DM. Motility of spermatozoa at surfaces. Reproduction<\/i> 2003; 126: 259\u2013270.","DOI":"10.1530\/rep.0.1260259"},{"key":"77","doi-asserted-by":"crossref","unstructured":"77. Elgeti J, Kaupp UB, Gompper G. Hydrodynamics of sperm cells near surfaces. Biophys J<\/i> 2010; 99: 1018\u20131026.","DOI":"10.1016\/j.bpj.2010.05.015"},{"key":"78","doi-asserted-by":"crossref","unstructured":"78. Ishimoto K, Gaffney EA. A study of spermatozoan swimming stability near a surface. J Theor Biol<\/i> 2014; 360: 187\u2013199.","DOI":"10.1016\/j.jtbi.2014.06.034"},{"key":"79","doi-asserted-by":"crossref","unstructured":"79. Dabiri M, Vasilescu SA, Fang G, Hyatt J, de Graaf SP, Warkiani ME. Biomimetic angular microfluidic platform for onsite selection of high-quality animal sperm. Cell Reports Physical Science<\/i> 2026; 7: 103028.","DOI":"10.1016\/j.xcrp.2025.103028"},{"key":"80","doi-asserted-by":"crossref","unstructured":"80. Fernandez-Hermida Y, Grande G, Menarguez M, Astorri AL, Azagra R. Proteomic Markers in Cervical Mucus. Protein Pept Lett<\/i> 2018; 25: 463\u2013471.","DOI":"10.2174\/0929866525666180418122705"},{"key":"81","doi-asserted-by":"crossref","unstructured":"81. Gipson IK. Mucins of the human endocervix. Frontiers in Bioscience: a journal of virtual library<\/i> 2001; 6: 1245\u20131255.","DOI":"10.2741\/A677"},{"key":"82","doi-asserted-by":"crossref","unstructured":"82. Soleilhavoup C, Riou C, Tsikis G, Labas V, Harichaux G, Kohnke P, Reynaud K, de Graaf SP, G\u00e9rard N, Druart X. Proteomes of the female genital tract during the oestrous cycle. Mol Cell Proteomics<\/i> 2016; 15: 93\u2013108.","DOI":"10.1074\/mcp.M115.052332"},{"key":"83","doi-asserted-by":"crossref","unstructured":"83. Abril-Parre\u00f1o L, Morgan J, Krogen\u00e6s A, Druart X, Cormican P, Gallagher ME, Reid C, Meade K, Saldova R, Fair S. Biochemical and molecular characterization of sialylated cervical mucins in sheep\u2020. Biol Reprod<\/i> 2022; 107: 419\u2013431.","DOI":"10.1093\/biolre\/ioac077"},{"key":"84","doi-asserted-by":"crossref","unstructured":"84. Bornh\u00f6fft KF, Rebl A, Gallagher ME, Viergutz T, Zlatina K, Reid C, Galuska SP. Sialylated cervical mucins inhibit the activation of neutrophils to form neutrophil extracellular traps in bovine in vitro<\/i> model. Front Immunol<\/i> 2019; 10: 2478.","DOI":"10.3389\/fimmu.2019.02478"},{"key":"85","doi-asserted-by":"crossref","unstructured":"85. Abril-Parre\u00f1o L, Wilkinson H, Krogen\u00e6s A, Morgan J, Gallagher ME, Reid C, Druart X, Fair S, Saldova R. Identification and characterization of O-linked glycans in cervical mucus as biomarkers of sperm transport: A novel sheep model. Glycobiology<\/i> 2022; 32: 23\u201335.","DOI":"10.1093\/glycob\/cwab085"},{"key":"86","doi-asserted-by":"crossref","unstructured":"86. Abril-Parre\u00f1o L, Druart X, Fair S, Krogenaes A. Metabolic signature of cervical mucus in ewe breeds with divergent cervical sperm transport: a focus on metabolites involved in amino acid metabolism. Metabolomics<\/i> 2023; 19: 59.","DOI":"10.1007\/s11306-023-02021-x"},{"key":"87","doi-asserted-by":"crossref","unstructured":"87. N\u00f8rsteb\u00f8 SF, Rodriguez-Campos S, Umu \u00d6CO, Abril-Parre\u00f1o L, Dalland M, Gilfillan GD, Fair S, Krogenaes A. The cervical microbiome of ewe breeds with known divergent fertility following artificial insemination with frozen-thawed semen. Sci Rep<\/i> 2025; 15: 14614.","DOI":"10.1038\/s41598-025-97735-4"},{"key":"88","doi-asserted-by":"crossref","unstructured":"88. Reinoso-Pel\u00e1ez EL, Puente-S\u00e1nchez F, Serrano M, Calvo JH, Ram\u00f3n M, Saura M. Characterization of bacterial communities of ewe\u2019s vaginal tract and its potential impact on reproductive efficiency. Anim Microbiome<\/i> 2025; 7: 48.","DOI":"10.1186\/s42523-025-00383-2"},{"key":"89","doi-asserted-by":"crossref","unstructured":"89. De Souza IS, Cooke RF, Mackey SJ, Minela T, Seekford ZK, Copani G, Gresse R, Harvey KM, Pohler KG, Vendramini JMB, Cappellozza BI. Effects of feeding a Bacillus-based probiotic on nutrient utilization and microbiota profile in the rumen and reproductive tract of beef heifers receiving a forage-based diet. J Anim Sci<\/i> 2026; 104: skaf411.","DOI":"10.1093\/jas\/skaf411"},{"key":"90","doi-asserted-by":"crossref","unstructured":"90. Pickett AT, Cooke RF, Mackey SJ, Brand\u00e3o AP, Colombo EA, Oliveira Filho RV, de Melo GD, Pohler KG, Poole RK. Shifts in bacterial communities in the rumen, vagina, and uterus of beef heifers receiving different levels of concentrate. J Anim Sci<\/i> 2022; 100: skac338.","DOI":"10.1093\/jas\/skac338"},{"key":"91","doi-asserted-by":"crossref","unstructured":"91. Sagheer M, Hoorn QA, Maldonado MB, Schalich KM, Ashrafi N, A Mimi R, Graham SF, Selvaraj V, Hansen PJ. Metabolomic profiling of neat uterine luminal fluid in cows: local enrichment and nutritional modulation. Vet Q<\/i> 2026; 46: 2611849.","DOI":"10.1080\/01652176.2025.2611849"},{"key":"92","doi-asserted-by":"crossref","unstructured":"92. Davies-Strickleton H, Pudney PD, Georgiades P, Waigh TA, Hodson NW, Ridley CE, Blanch EW, Thornton DJ. Reorganisation of the salivary mucin network by dietary components: insights from green tea polyphenols. PLoS One<\/i> 2014; 9: e108372.","DOI":"10.1371\/journal.pone.0108372"},{"key":"93","doi-asserted-by":"crossref","unstructured":"93. Lehrer AR, Schindler H. Uterine motility of the ewe during the estrous cycle. J Anim Sci<\/i> 1974; 38: 86\u201388.","DOI":"10.2527\/jas1974.38186x"},{"key":"94","doi-asserted-by":"crossref","unstructured":"94. Garcia-Villar R, Toutain PL, More J, Ruckebusch Y. Spontaneous motility of the cervix in cyclic and ovariectomized ewes and changes induced by exogenous hormones. J Reprod Fertil<\/i> 1982; 66: 317\u2013326.","DOI":"10.1530\/jrf.0.0660317"},{"key":"95","unstructured":"95. Prud\u2019Homme MJ, Pele B. Electromyographic activity of the uterus in the ewe during the sexual season: comparison of natural estrus and estrus induced by progesterone alone or with PMSG supplmentation. Reprod Nutr Dev<\/i> 1980; 1984: 33\u201344."},{"key":"96","doi-asserted-by":"crossref","unstructured":"96. Hawk HW. Hormonal control of changes in the direction of uterine contractions in the estrous ewe. Biol Reprod<\/i> 1975; 12: 423\u2013430.","DOI":"10.1095\/biolreprod12.3.423"},{"key":"97","doi-asserted-by":"crossref","unstructured":"97. Lye SJ, Porter DG. Demonstration that progesterone \u2018blocks\u2019 uterine activity in the ewe in vivo by a direct action on the myometrium. J Reprod Fertil<\/i> 1978; 52: 87\u201394.","DOI":"10.1530\/jrf.0.0520087"},{"key":"98","doi-asserted-by":"crossref","unstructured":"98. Toutain PL, Marnet PG, Laurentie MP, Garcia-Villar R, Ruckebusch Y. Direction of uterine contractions during estrus in ewes: a reevaluation. Am J Physiol<\/i> 1985; 249: R410\u2013R416.","DOI":"10.1152\/ajpregu.1985.249.4.R410"},{"key":"99","doi-asserted-by":"crossref","unstructured":"99. Camerino C. The long way of oxytocin from the uterus to the heart in 70 years from its discovery. Int J Mol Sci<\/i> 2023; 24: 2556.","DOI":"10.3390\/ijms24032556"},{"key":"100","doi-asserted-by":"crossref","unstructured":"100. Fairclough RJ, Lau TM. Hormonal control of concentrations of endometrial oxytocin receptors in the ewe. Reprod Fertil Dev<\/i> 1992; 4: 313\u2013320.","DOI":"10.1071\/RD9920313"},{"key":"101","doi-asserted-by":"crossref","unstructured":"101. Sayre BL, Lewis GS. Cervical dilation with exogenous oxytocin does not affect sperm movement into the oviducts in ewes. Theriogenology<\/i> 1996; 45: 1523\u20131533.","DOI":"10.1016\/0093-691X(96)00120-3"},{"key":"102","doi-asserted-by":"crossref","unstructured":"102. Sayre BL, Lewis GS. Fertility and ovum fertilization rate after laparoscopic or transcervical intrauterine artificial insemination of oxytocin-treated ewes. Theriogenology<\/i> 1997; 48: 267\u2013275.","DOI":"10.1016\/S0093-691X(97)84074-5"},{"key":"103","doi-asserted-by":"crossref","unstructured":"103. Alvarez M, Anel-Lopez L, Boixo JC, Chamorro C, Neila-Montero M, Montes-Garrido R, de Paz P, Anel L. Current challenges in sheep artificial insemination: A particular insight. Reprod Domest Anim<\/i> 2019; 54(Suppl 4): 32\u201340.","DOI":"10.1111\/rda.13523"},{"key":"104","doi-asserted-by":"crossref","unstructured":"104. King ME, McKelvey WA, Dingwall WS, Matthews KP, Gebbie FE, Mylne MJ, Stewart E, Robinson JJ. Lambing rates and litter sizes following intrauterine or cervical insemination of frozen\/thawed semen with or without oxytocin administration. Theriogenology<\/i> 2004; 62: 1236\u20131244.","DOI":"10.1016\/j.theriogenology.2004.01.009"},{"key":"105","doi-asserted-by":"crossref","unstructured":"105. Falchi L, Taema M, La Clanche S, Scaramuzzi RJ. The pattern of cervical penetration and the effect of topical treatment with prostaglandin and\/or FSH and oxytocin on the depth of cervical penetration in the ewe during the peri-ovulatory period. Theriogenology<\/i> 2012; 78: 376\u2013384.","DOI":"10.1016\/j.theriogenology.2012.02.017"},{"key":"106","doi-asserted-by":"crossref","unstructured":"106. Houdeau E, Raynal P, Marnet PG, Germain G, Morm\u00e8de P, Rossano B, Monnerie R, Prud\u2019homme MJ. Plasma levels of cortisol and oxytocin, and uterine activity after cervical artificial insemination in the ewe. Reprod Nutr Dev<\/i> 2002; 42: 381\u2013392.","DOI":"10.1051\/rnd:2002033"},{"key":"107","doi-asserted-by":"crossref","unstructured":"107. Pichat J, Iglesias JE, Yousry T, Ourselin S, Modat M. A survey of methods for 3D histology reconstruction. Med Image Anal<\/i> 2018; 46: 73\u2013105.","DOI":"10.1016\/j.media.2018.02.004"},{"key":"108","doi-asserted-by":"crossref","unstructured":"108. Tielemans B, Marain NF, Kerstens A, Peredo N, Coll-Llad\u00f3 M, Gritti N, de Villemagne P, Dorval P, Geudens V, Orlitov\u00e1 M, Munck S, Leszczy\u0144ski B, Swoger J, Velde GV. Multiscale three-dimensional evaluation and analysis of murine lung vasculature from macro- to micro-structural level. Pulm Circ<\/i> 2025; 15: e70038.","DOI":"10.1002\/pul2.70038"},{"key":"109","doi-asserted-by":"crossref","unstructured":"109. Karimi A, Jiang X, Abbaspourrad A. Navigation and selection of spermatozoa in a radial flow microfluidic device. Lab Chip<\/i> 2026; 26: 403\u2013414.","DOI":"10.1039\/D5LC00778J"},{"key":"110","doi-asserted-by":"crossref","unstructured":"110. Wu T, Wu Y, Yan J, Zhang J, Wang S. Microfluidic chip as a promising evaluation method in assisted reproduction: A systematic review. Bioeng Transl Med<\/i> 2023; 9: e10625.","DOI":"10.1002\/btm2.10625"},{"key":"111","doi-asserted-by":"crossref","unstructured":"111. Hyakutake T, Higashiyama D, Tsuchiya T. Prediction of sperm motion behavior in microfluidic channel using sperm swimming model. J Biomech<\/i> 2024; 176: 112336.","DOI":"10.1016\/j.jbiomech.2024.112336"},{"key":"112","doi-asserted-by":"crossref","unstructured":"111. Kantsler V, Dunkel J, Blayney M, Goldstein RE. Rheotaxis facilitates upstream navigation of mammalian sperm cells. eLife<\/i> 2014; 3: e02403.","DOI":"10.7554\/eLife.03521"},{"key":"113","doi-asserted-by":"crossref","unstructured":"113. Singh RP. Progress and challenges in designing dynamic in vitro<\/i> gastric models to study food digestion. Front Nutr<\/i> 2024; 11: 1399534.","DOI":"10.3389\/fnut.2024.1399534"},{"key":"114","doi-asserted-by":"crossref","unstructured":"114. Li Y, Kong F. Simulating human gastrointestinal motility in dynamic in vitro models. Compr Rev Food Sci Food Saf<\/i> 2022; 21: 3804\u20133833.","DOI":"10.1111\/1541-4337.13007"},{"key":"115","doi-asserted-by":"crossref","unstructured":"115. Ingber DE. Human organs-on-chips for disease modelling, drug development and personalized medicine. Nat Rev Genet<\/i> 2022; 23: 467\u2013491.","DOI":"10.1038\/s41576-022-00466-9"},{"key":"116","doi-asserted-by":"crossref","unstructured":"116. Deng ZM, Dai FF, Wang RQ, Deng HB, Yin TL, Cheng YX, Chen GT. Organ-on-a-chip: future of female reproductive pathophysiological models. J Nanobiotechnology<\/i> 2024; 22: 455.","DOI":"10.1186\/s12951-024-02651-w"},{"key":"117","doi-asserted-by":"crossref","unstructured":"117. Pan M, Chen H, Deng K, Xiao K. Organ-on-a-chip models of the female reproductive system: current progress and future perspectives. Micromachines (Basel)<\/i> 2025; 16: 1125.","DOI":"10.3390\/mi16101125"},{"key":"118","doi-asserted-by":"crossref","unstructured":"118. Richards HA, Eastman AJ, Miller DR, Cliffel DE. Reproductive organ on-a-chip technologies and assessments of the fetal-maternal interface. Frontiers n Lab on a Chip Technologies<\/i> 2024; 3.","DOI":"10.3389\/frlct.2024.1449303"},{"key":"119","doi-asserted-by":"crossref","unstructured":"119. Tantengco OAG, Richardson LS, Medina PMB, Han A, Menon R. Organ-on-chip of the cervical epithelial layer: A platform to study normal and pathological cellular remodeling of the cervix. FASEB J<\/i> 2021; 35: e21463.","DOI":"10.1096\/fj.202002590RRR"},{"key":"120","doi-asserted-by":"crossref","unstructured":"120. Papadopoulos Z. The role of the cytokine TNF-\u03b1 in choroidal neovascularization: a systematic review. Eye (Lond)<\/i> 2024; 38: 25\u201332.","DOI":"10.1038\/s41433-023-02634-5"},{"key":"121","doi-asserted-by":"crossref","unstructured":"121. Izadifar Z, Cotton J, Chen S, Horvath V, Stejskalova A, Gulati A, LoGrande NT, Budnik B, Shahriar S, Doherty ER, Xie Y, To T, Gilpin SE, Sesay AM, Goyal G, Lebrilla CB, Ingber DE. Mucus production, host-microbiome interactions, hormone sensitivity, and innate immune responses modeled in human cervix chips. Nat Commun<\/i> 2024; 15: 4578.","DOI":"10.1038\/s41467-024-48910-0"},{"key":"122","doi-asserted-by":"crossref","unstructured":"122. Izadifar Z, Ingber DE. A Human Cervix Chip for Preclinical Studies of Female Reproductive Biology. Bio Protoc<\/i> 2025; 15: e5262.","DOI":"10.21769\/BioProtoc.5262"},{"key":"123","doi-asserted-by":"crossref","unstructured":"123. McKinley JP, Montes AR, Wang MN, Kamath AR, Jimenez G, Lim J, Marathe SA, Mofrad MRK, O\u2019Connell GD. Design of a flexing organ-chip to model in situ<\/i> loading of the intervertebral disc. Biomicrofluidics<\/i> 2022; 16: 054111.","DOI":"10.1063\/5.0103141"},{"key":"124","doi-asserted-by":"crossref","unstructured":"124. Mohamed R, John R. Production and use of plastinated anatomical specimens as teaching and learning tools in veterinary gross anatomy in the Caribbean. J Adv Vet Anim Res<\/i> 2018; 5: 44\u201352.","DOI":"10.5455\/javar.2018.e245"}],"container-title":["Journal of Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/3\/72_2026-021\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T03:27:30Z","timestamp":1781926050000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jrd\/72\/3\/72_2026-021\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":124,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.1262\/jrd.2026-021","relation":{},"ISSN":["0916-8818","1348-4400"],"issn-type":[{"value":"0916-8818","type":"print"},{"value":"1348-4400","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"2026-021"}}