{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T01:17:14Z","timestamp":1790212634909,"version":"4.1.0"},"reference-count":38,"publisher":"Japanese Society of Microbial Ecology","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb. Environ.","Microbes Environ.","Microbes and environments"],"published-print":{"date-parts":[[2013]]},"DOI":"10.1264\/jsme2.me12154","type":"journal-article","created":{"date-parts":[[2013,1,29]],"date-time":"2013-01-29T22:59:32Z","timestamp":1359500372000},"page":"135-140","source":"Crossref","is-referenced-by-count":76,"title":["Pathogenic and Saprophytic &lt;i&gt;Leptospira&lt;\/i&gt; Species in Water and Soils from Selected Urban Sites in Peninsular Malaysia"],"prefix":"10.1264","volume":"28","author":[{"given":"Douadi","family":"Benacer","sequence":"first","affiliation":[{"name":"Laboratory of Biomedical Science and Molecular Microbiology, Institute of Graduate Studies, University of Malaya"},{"name":"Institute of Biological Sciences, Faculty of Science, University of Malaya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei Yee","family":"Woh","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Faculty of Science, University of Malaya"},{"name":"Laboratory of Biomedical Science and Molecular Microbiology, Institute of Graduate Studies, University of Malaya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siti Nursheena","family":"Mohd Zain","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Faculty of Science, University of Malaya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fairuz","family":"Amran","sequence":"additional","affiliation":[{"name":"Bacteriology Unit, Institute for Medical Research"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwai Lin","family":"Thong","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Faculty of Science, University of Malaya"},{"name":"Laboratory of Biomedical Science and Molecular Microbiology, Institute of Graduate Studies, University of Malaya"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"336","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1. Alexander, A.D., L.B. Evans, M.F. Baker, H.J. Baker, D. Ellison, and M. Marriapan. 1975. Pathogenic leptospires isolated from Malaysian surface waters. Appl. Environ. Microbiol. 29:30&ndash;33.","DOI":"10.1128\/AEM.29.1.30-33.1975"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Bahaman, A.R., and A.L. Ibrahim. 1988. A review of leptospirosis in Malaysia. Vet. Res. Commun. 12:179&ndash;189.","DOI":"10.1007\/BF00362799"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. Baker, M.F., and H.J. Baker. 1970. Pathogenic <i>Leptospira<\/i> in Malaysian surface waters. Am. Trop. Med. Hyg. 19:485&ndash;492.","DOI":"10.4269\/ajtmh.1970.19.485"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Barragan, V.A., M.E. Mejia, A. Travez, S. Zapata, R.A. Hartskeerl, D.A. Haake, and G.A. Trueba. 2011. Interactions of <i>Leptospira<\/i> with environmental bacteria from surface water. J. Curr. Microbiol. 62:1802&ndash;1806.","DOI":"10.1007\/s00284-011-9931-3"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Bharti, A.R., J.E. Nally, J.N. Ricaldi, et al. 2003. Leptospirosis: a zoonotic disease of global importance. Lancet Infect. Dis. 3:757&ndash;771.","DOI":"10.1016\/S1473-3099(03)00830-2"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Bomfim, M.R.Q., E.F. Barbosa-Stancioli, and M.C. Koury. 2008. Detection of pathogenic leptospires in urine from naturally infected cattle by nested PCR. Vet. J. 178:251&ndash;256.","DOI":"10.1016\/j.tvjl.2007.07.029"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Chakraborty, A., S. Miyahara, S.Y.A.M. Villanueva, M. Saito, N.G. Gloriani, and S. Yoshida. 2011. A novel combination of selective agents for isolation of <i>Leptospira<\/i> species. Microbiol. Immun. 55:494&ndash;501.","DOI":"10.1111\/j.1348-0421.2011.00347.x"},{"key":"8","unstructured":"8. Fletcher, W. 1928. Recent work on leptospirosis, tustsugamushi disease and tropical typhus in the Federal Malay States. T. Roy. Soc. Trop. Med. H. 21:265&ndash;288."},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Fonseca, C.A., M.M.G. Teixeira, E.C. Romero, F.M. Tengan, M.V. da Silva, and M.A. Shikanai-Yasuda. 2006. <i>Leptospira<\/i> DNA detection for the diagnosis of human leptospirosis. J. Infect. 52:15&ndash;22.","DOI":"10.1016\/j.jinf.2005.02.022"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Galloway, R.L., and P.N. Levett. 2008. Evaluation of a modified pulsed-field gel electrophoresis approach for the identification of <i>Leptospira<\/i> serovars. Am. J. Trop. Med. Hyg. 78:628&ndash;632.","DOI":"10.4269\/ajtmh.2008.78.628"},{"key":"11","unstructured":"11. Gordon-Smith, C.E., L.H. Turner, J.L. Harrison, and J.C. Broom. 1961 Animal leptospirosis in Malaya: 1. Methods, zoogeographical background, and broad analysis of results. B. World. Health Organ. 24:5&ndash;21."},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Gravekamp, C., H. Van de Kemp, M. Franzen, D. Carrington, G.J. Schoone, G.J.J.M. Van Eys, C.O.R. Everard, R.A. Hartskeerl, and W.J. Terpstra. 1993. Detection of seven species of pathogenic leptospires by PCR using two sets of primers. J. Gen. Microbiol. 139:1691&ndash;1700.","DOI":"10.1099\/00221287-139-8-1691"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Henry, R.A., and R.C. Johnson. 1978. Distribution of the genus <i>Leptospira<\/i> in soil and water. Appl. Environ. Microbiol. 35:492&ndash;499.","DOI":"10.1128\/AEM.35.3.492-499.1978"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Herrmann, J.L., E. Bellenger, P. Perolat, G. Baranton, and I. Saint Girons. 1992. Pulsed fieldgelelectrophoresis of <i>Not<\/i>I digests of leptospiral DNA: a new rapid method of serovar identification. J. Clin. Microbiol. 30:1696&ndash;1702.","DOI":"10.1128\/JCM.30.7.1696-1702.1992"},{"key":"15","unstructured":"15. Johnson, M.A.S., H. Smith, P. Joseph, et al. 2004. Environmental exposure and leptospirosis, Peru. Emerg. Infect. Dis. 10:1016&ndash;1022."},{"key":"16","unstructured":"16. Khairani-Bejo, S., A.R. Bahaman, M. Zamri-Saad, and A.R. Mutalib. 2004. The survival of <i>Leptospira interrogans<\/i> serovar Hardjo in the Malaysian environment. J. Anim. Vet. Adv. 3:123&ndash;129."},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. Ko, A.I., M. Galvao Reis, C.M. Ribeiro Dourado, W.D. Johnson, and L.W. Riley. 1999. Urban epidemic of severe leptospirosis in Brazil. Lancet. 354:820&ndash;825.","DOI":"10.1016\/S0140-6736(99)80012-9"},{"key":"18","unstructured":"18. Koay, T.K., S. Nirmal, L. Noitie, and E. Tan. 2004. An epidemiological investigation of an outbreak of leptospirosis associated with swimming, Beaufort, Sabah. Med. J. Malaysia 59:455&ndash;459."},{"key":"19","doi-asserted-by":"crossref","unstructured":"19. Levett, P.N. 2001. Leptospirosis. Clin. Microbiol. Rev. 14:296&ndash;326.","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"key":"20","unstructured":"20. Lim, J.K., V.A. Murugaiyah, A. Ramli, H. Abdul Rahman, N. Mohamed, N. Shamsudin, and J.C. Tan. 2011. A case study: leptospirosis in Malaysia. Webmed Central Infect. Dis. 2:1&ndash;12."},{"key":"21","doi-asserted-by":"crossref","unstructured":"21. Merien, F., P. Amouriaux, P. Perolat, G. Baranton, and I. Saint Girons. 1992. Polymerase Chain Reaction for detection of <i>Leptospira<\/i> spp. in clinical samples. J. Clin. Microbiol. 30:2219&ndash;2224.","DOI":"10.1128\/JCM.30.9.2219-2224.1992"},{"key":"22","unstructured":"22. Mohammed, H., C. Nozha, K. Hakim, F. Abdelaziz, and B. Rekia. 2011. <i>Leptospira<\/i>: morphology, classification and pathogenesis. J. Bacteriol. Parasitol. 2:120&ndash;123."},{"key":"23","doi-asserted-by":"crossref","unstructured":"23. Murgia, R., N. Riquelme, G. Baranton, and M. Cinco. 1997. Oligonucleotides specific for pathogenic and saprophytic <i>Leptopsira<\/i> occurring in water. FEMS Microbiol. Lett. 148:27&ndash;34.","DOI":"10.1016\/S0378-1097(97)00008-6"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24. Rahelinirina, S., A. Leon, R.A. Hartskeerl, et al. 2010. First isolation and direct evidence for the existence of large small-mammal reservoirs of <i>Leptospira<\/i> sp. in Madagascar. PLoS ONE 5:e14111.","DOI":"10.1371\/journal.pone.0014111"},{"key":"25","unstructured":"25. Ridzlan, F.R., A.R. Bahaman, S. Khairani-Bejo, and A.R. Mutalib. 2010. Detection of pathogenic <i>Leptospira<\/i> from selected environment in Kelantan and Terengganu, Malaysia. Trop. Biomed. 27:632&ndash;638."},{"key":"26","unstructured":"26. Sapian, M., M.T. Khairi, S.H. How, R. Rajalingam, K. Sahhir, A. Norazah, V. Khebir, and A.R. Jamalludin. 2012. Outbreak of melioidosis and leptospirosis co-infection following a rescue operation. Med. J. Malaysia 67:293&ndash;297."},{"key":"27","unstructured":"27. Sejvar, J., E. Bancroft, K. Winthrop, et al. 2003. Leptospirosis in &ldquo;Eco-Challenge&rdquo; athletes, Malaysian Borneo. Emerg. Infect. Dis. 9:702&ndash;707."},{"key":"28","unstructured":"28. Sharma, M., and A.Yadav. 2008. Leptospirosis: epidemiology, diagnosis, and control. J Infect. Dis. Antimicrob. Agents. 25:93&ndash;103."},{"key":"29","unstructured":"29. Shafei, M.N., M.R. Sulong, N.A. Yaacob, H. Hassan, W.M.Z. Wan Mohammad, A. Daud, Z. Ismail, and M.R Abdullah. 2012. Seroprevalence of leptospirosis among town service workers on northeastern State of Malaysia. IJCRIMPH. 4:395&ndash;403."},{"key":"30","unstructured":"30. Slack, A.T., S. Khairani-Bejo, M.L. Symonds, et al. 2009. <i>Leptospira kmetyi<\/i> sp. nov., isolated from an environmental source in Malaysia. Int. J. Syst. Evol. Microbiol. 59:705&ndash;708."},{"key":"31","unstructured":"31. Tan, D.S.K. 1970. Clinical leptospirosis in West Malaysia (1958&ndash;1968). Southeast Asian J. Trop. Med. Publ. Health. 1:102&ndash;111."},{"key":"32","unstructured":"32. Tan, D.S.K., A. Suleiman, and S. Jeyaindran. 1986. 16 cases of acute renal failure due to leptospirosis. Med. J. Malaysia 41:152&ndash;155."},{"key":"33","doi-asserted-by":"crossref","unstructured":"33. Thaipadungpanit, J., V. Wuthiekanun, W. Chierakul, et al. 2007. A dominant clone of <i>Leptospira interrogans<\/i> associated with an outbreak of human leptospirosis in Thailand. PLoS Negl. Trop. Dis. 1:e56.","DOI":"10.1371\/journal.pntd.0000056"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34. Thaipadungpanit, J., W. Chierakul, V. Wuthiekanun, et al. 2011. Diagnostic accuracy of real-time PCR assays targeting 16S rRNA and lipl32 genes for human leptospirosis in Thailand: a case-control study. PLoS ONE 6:e16236.","DOI":"10.1371\/journal.pone.0016236"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35. Victoriano, A.F.B., L.D. Smythe, N. Gloriani-Barzaga, et al. 2009. Leptospirosis in the Asia Pacific region. BMC Infect. Dis. 9:147&ndash;156.","DOI":"10.1186\/1471-2334-9-147"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36. Vital-Brazil, J., I. Balassiano, F. Oliveira, A. Costa, L. Hillen, and M. Pereira. 2012. Multiplex PCR-based detection of <i>Leptospira<\/i> in environmental water samples obtained from a slum settlement. Mem. Inst. Oswaldo Cruz. 105:353&ndash;355.","DOI":"10.1590\/S0074-02762010000300020"},{"key":"37","unstructured":"37. Wangroongsarb, P., W. Petkanchanapong, S. Yasaeng, A. Imvithaya, and P. Naigovit. 2002. Survey of leptospirosis among rodents in epidemic areas of Thailand. J. Trop. Med. 25:55&ndash;58."},{"key":"38","unstructured":"38. World Health Organization. 2003. Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control. Malta: World Health Organization and International Leptospirosis Society."}],"container-title":["Microbes and Environments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jsme2\/28\/1\/28_ME12154\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,26]],"date-time":"2021-03-26T02:35:34Z","timestamp":1616726134000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jsme2\/28\/1\/28_ME12154\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013]]}},"URL":"https:\/\/doi.org\/10.1264\/jsme2.me12154","relation":{},"ISSN":["1342-6311","1347-4405"],"issn-type":[{"value":"1342-6311","type":"print"},{"value":"1347-4405","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013]]}}}