{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T05:09:47Z","timestamp":1789189787323,"version":"build-2803163510"},"reference-count":50,"publisher":"Japanese Society of Microbial Ecology","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb. Environ.","Microbes Environ.","Microbes and environments"],"published-print":{"date-parts":[[2026]]},"DOI":"10.1264\/jsme2.me26022","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:13:21Z","timestamp":1788992001000},"page":"n\/a","source":"Crossref","is-referenced-by-count":0,"title":["Inoculation with &lt;i&gt;Bradyrhizobium ottawaense&lt;\/i&gt; Effectively Reduces N&lt;sub&gt;2&lt;\/sub&gt;O Emissions from Degraded Soybean Nodules in Andosols Dominated by &lt;i&gt;nosZ&lt;\/i&gt;-lacking Bradyrhizobia"],"prefix":"10.1264","volume":"41","author":[{"given":"Misa","family":"Tsubokura","sequence":"first","affiliation":[{"name":"Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shintaro","family":"Hara","sequence":"additional","affiliation":[{"name":"Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsubasa","family":"Ohbayashi","sequence":"additional","affiliation":[{"name":"Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Takeda","sequence":"additional","affiliation":[{"name":"Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuma","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruka","family":"Ukita","sequence":"additional","affiliation":[{"name":"Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miho","family":"Fujimori","sequence":"additional","affiliation":[{"name":"Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Kakizaki","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences, Tohoku University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Itakura","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences, Tohoku University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiwamu","family":"Minamisawa","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences, Tohoku University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Akiyama","sequence":"additional","affiliation":[{"name":"Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"336","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"Akiyama, H., Hoshino, Y.T., Itakura, M., Shimomura, Y., Wang, Y., Yamamoto, A., et al. (2016) Mitigation of soil N<sub>2<\/sub>O emission by inoculation with a mixed culture of indigenous <i>Bradyrhizobium diazoefficiens<\/i>. Sci Rep               6: 32869.","DOI":"10.1038\/srep32869"},{"key":"2","doi-asserted-by":"crossref","unstructured":"Akiyama, H., Yamamoto, A., Uchida, Y., Hoshino, Y.T., Tago, K., Wang, Y., and Hayatsu, M. (2020) Effect of low C\/N crop residue input on N<sub>2<\/sub>O, NO, and CH<sub>4<\/sub> fluxes from Andosol and Fluvisol fields. Sci Total Environ               713: 136677.","DOI":"10.1016\/j.scitotenv.2020.136677"},{"key":"3","doi-asserted-by":"crossref","unstructured":"Akiyama, H. (2024) Methane and nitrous oxide emissions from agricultural fields in Japan and mitigation options: a review. Soil Sci Plant Nutr               70: 79\u201387.","DOI":"10.1080\/00380768.2023.2298782"},{"key":"4","doi-asserted-by":"crossref","unstructured":"Alves, B.J.R., Smith, K.A., Flores, R.A., Cardoso, A.S., Oliveira, W.R.D., Jantalia, C.P., et al. (2012) Selection of the most suitable sampling time for static chambers for the estimation of daily mean N<sub>2<\/sub>O flux from soils. Soil Biol Biochem               46: 129\u2013135.","DOI":"10.1016\/j.soilbio.2011.11.022"},{"key":"5","doi-asserted-by":"crossref","unstructured":"Arachchige, P.S.P., Rosso, L.H.M., Hansel, F.D., Ramundo, B., Torres, A.R., Asebedo, R., et al. (2020) Temporal biological nitrogen fixation pattern in soybean inoculated with <i>Bradyrhizobium<\/i>. Agrosyst Geosci Environ               3: e20079.","DOI":"10.1002\/agg2.20079"},{"key":"6","doi-asserted-by":"crossref","unstructured":"Cole, M.A., and Elkan, G.H. (1973) Transmissible resistance to penicillin G, neomycin, and chloramphenicol in <i>Rhizobium japonicum<\/i>. Antimicrob Agents Chemother               4: 248\u2013253.","DOI":"10.1128\/AAC.4.3.248"},{"key":"7","doi-asserted-by":"crossref","unstructured":"Davidson, E.A. (1993) Soil water content and the ratio of nitrous oxide to nitric oxide emitted from soil. In <i>Biogeochemistry of Global Change: Radiatively Active Trace Gases<\/i>. Oremland, R.S. (ed). Boston, MA: Springer US, pp. 369\u2013386.","DOI":"10.1007\/978-1-4615-2812-8_20"},{"key":"8","unstructured":"Forster, P., Storelvmo, T., Armour, K., Collins, W., Dufresne, J-L., Frame, D., et al. (2021) The Earth\u2019s energy budget, climate feedbacks, and climate sensitivity. In <i>Climate Change 2021: The Physical Science Basis. Contribution of Working Group I to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change<\/i>. Masson-Delmotte, V., Zhai, P., Pirani, A., Connors, S.L., P\u00e9an, C., Berger, S., et al. (eds). Cambridge, United Kingdom and New York, NY: Cambridge University Press, pp. 923\u20131054."},{"key":"9","doi-asserted-by":"crossref","unstructured":"Hara, S., Kakizaki, K., Bamba, M., Itakura, M., Sugawara, M., Suzuki, A., et al. (2024) Does rhizobial inoculation change the microbial community in field soils? A comparison with agricultural land-use changes. Microbes Environ               39: ME24006.","DOI":"10.1264\/jsme2.ME24006"},{"key":"10","doi-asserted-by":"crossref","unstructured":"Hassan, M.U., Aamer, M., Mahmood, A., Awan, M.I., Barbanti, L., Seleiman, M.F., et al. (2022) Management strategies to mitigate N<sub>2<\/sub>O emissions in agriculture. Life (Basel)               12: 439.","DOI":"10.3390\/life12030439"},{"key":"11","doi-asserted-by":"crossref","unstructured":"H\u00e9nault, C., Barbier, E., Hartmann, A., and Revellin, C. (2022) New insights into the use of rhizobia to mitigate soil N<sub>2<\/sub>O emissions. Agriculture (Basel, Switz)               12: 271.","DOI":"10.3390\/agriculture12020271"},{"key":"12","doi-asserted-by":"crossref","unstructured":"Hou, H., Chen, H., Cai, H., Yang, F., Li, D., and Wang, F. (2016) CO<sub>2<\/sub> and N<sub>2<\/sub>O emissions from Lou soils of greenhouse tomato fields under aerated irrigation. Atmos Environ               132: 69\u201376.","DOI":"10.1016\/j.atmosenv.2016.02.027"},{"key":"13","doi-asserted-by":"crossref","unstructured":"Huang, Y., Zou, J., Zheng, X., Wang, Y., and Xu, X. (2004) Nitrous oxide emissions as influenced by amendment of plant residues with different C: N ratios. Soil Biol Biochem               36: 973\u2013981.","DOI":"10.1016\/j.soilbio.2004.02.009"},{"key":"14","doi-asserted-by":"crossref","unstructured":"Inaba, S., Ikenishi, F., Itakura, M., Kikuchi, M., Eda, S., Chiba, N., et al. (2012) N<sub>2<\/sub>O emission from degraded soybean nodules depends on denitrification by <i>Bradyrhizobium japonicum<\/i> and other microbes in the rhizosphere. Microbes Environ               27: 470\u2013476.","DOI":"10.1264\/jsme2.ME12100"},{"key":"15","doi-asserted-by":"crossref","unstructured":"Itakura, M., Uchida, Y., Akiyama, H., Hoshino, Y.T., Shimomura, Y., Morimoto, S., et al. (2013) Mitigation of nitrous oxide emissions from soils by <i>Bradyrhizobium japonicum<\/i> inoculation. Nat Clim Change               3: 208\u2013212.","DOI":"10.1038\/nclimate1734"},{"key":"16","doi-asserted-by":"crossref","unstructured":"Itakura, M., Kakizaki, K., Suzuki, A., Okubo, S., Kato, H., Sugawara, M., et al. (2026) Screening of <i>Bradyrhizobium ottawaense<\/i> with high N<sub>2<\/sub>O-reducing activity from soybean nodules in Japan. Microbes Environ               41: ME25062.","DOI":"10.1264\/jsme2.ME25062"},{"key":"17","doi-asserted-by":"crossref","unstructured":"Itakura, M., and Minamisawa, K. (2026) Kinetics of Nitrous Oxide (N<sub>2<\/sub>O)-reducing Activity of <i>Bradyrhizobium ottawaense<\/i> by an Automated Analysis. Microbes Environ               41: ME25070.","DOI":"10.1264\/jsme2.ME25070"},{"key":"18","doi-asserted-by":"crossref","unstructured":"Kim, Y.H., Nakayama, N., Nakamura, T., Takahashi, M., Shimada, S., and Arihara, J. (2005) NO and N<sub>2<\/sub>O emissions from fields in the different nodulated genotypes of soybean. Jpn J Crop Sci               74: 427\u2013430.","DOI":"10.1626\/jcs.74.427"},{"key":"19","doi-asserted-by":"crossref","unstructured":"Melissa, O., Cristian, A., Sheila, C., Vanina, M., Daniela, T., Gabriela, P., et al. (2022) Evaluation of nitrous oxide emission by soybean inoculated with <i>Bradyrhizobium<\/i> strains commonly used as inoculants in South America. Plant Soil               472: 311\u2013328.","DOI":"10.1007\/s11104-021-05242-y"},{"key":"20","doi-asserted-by":"crossref","unstructured":"Menegat, S., Ledo, A., and Tirado, R. (2022) Greenhouse gas emissions from global production and use of nitrogen synthetic fertilisers in agriculture. Sci Rep               12: 14490.","DOI":"10.1038\/s41598-022-18773-w"},{"key":"21","doi-asserted-by":"crossref","unstructured":"Minakata, C., Wasai-Hara, S., Fujioka, S., Sano, S., and Matsumura, A. (2023) Unique rhizobial communities dominated by <i>Bradyrhizobium liaoningense<\/i> and <i>Bradyrhizobium ottawaense<\/i> were found in vegetable soybean nodules in Osaka Prefecture, Japan. Microbes Environ               38: ME22081.","DOI":"10.1264\/jsme2.ME22081"},{"key":"22","doi-asserted-by":"crossref","unstructured":"Moriuchi, M., Kuzunuki, K., Ikenishi, F., Sameshima, R., Nakagiri, A., Toyoda, S., et al. (2025) <i>Fusarium<\/i> fungi produce nitrous oxide (N<sub>2<\/sub>O) from nitrite (NO<sub>2<\/sub><sup>\u2013<\/sup>) in a model pot system simulating the soybean rhizosphere. Microbes Environ               40: ME24092.","DOI":"10.1264\/jsme2.ME24092"},{"key":"23","doi-asserted-by":"crossref","unstructured":"Nishida, H., Itakura, M., Win, K.T., Li, F., Kakizaki, K., Suzuki, A., et al. (2025) Genetic design of soybean hosts and bradyrhizobial endosymbionts reduces N<sub>2<\/sub>O emissions from soybean rhizosphere. Nat Commun               16: 8023.","DOI":"10.1038\/s41467-025-63223-6"},{"key":"24","unstructured":"R Core Team (2023) R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing. URL https:\/\/www.R-project.org\/"},{"key":"25","doi-asserted-by":"crossref","unstructured":"Rochette, P., Angers, D.A., B\u00e9langer, G., Chantigny, M.H., Pr\u00e9vost, D., and L\u00e9vesque, G. (2004) Emissions of N<sub>2<\/sub>O from alfalfa and soybean crops in eastern Canada. Soil Sci Soc Am J               68: 493\u2013506.","DOI":"10.2136\/sssaj2004.4930"},{"key":"26","doi-asserted-by":"crossref","unstructured":"Saeki, Y., Aimi, N., Tsukamoto, S., Yamakawa, T., Nagatomo, Y., and Akao, S. (2006) Diversity and geographical distribution of indigenous soybean-nodulating bradyrhizobia in Japan. Soil Sci Plant Nutr               52: 418\u2013426.","DOI":"10.1111\/j.1747-0765.2006.00050.x"},{"key":"27","doi-asserted-by":"crossref","unstructured":"Saeki, Y., Shiro, S., Tajima, T., Yamamoto, A., Sameshima-Saito, R., Sato, T., and Yamakawa, T. (2013) Mathematical ecology anal\u00adysis of geographical distribution of soybean-nodulating bradyrhizobia in Japan. Microbes Environ               28: 470\u2013478.","DOI":"10.1264\/jsme2.ME13079"},{"key":"28","doi-asserted-by":"crossref","unstructured":"Shiina, Y., Itakura, M., Choi, H., Saeki, Y., Hayatsu, M., and Minamisawa, K. (2014) Relationship between soil type and N<sub>2<\/sub>O reductase genotype (<i>nosZ<\/i>) of indigenous soybean bradyrhizobia: <i>nosZ<\/i>-minus populations are dominant in Andosols. Microbes Environ               29: 420\u2013426.","DOI":"10.1264\/jsme2.ME14130"},{"key":"29","doi-asserted-by":"crossref","unstructured":"Shinjo, H., and Takata, Y. (2021) Soil classification and distribution. In <i>The Soils of Japan<\/i>. Hatano, R., Shinjo, H., and Takata, Y. (eds). Singapore: Springer Singapore. pp. 53\u201368.","DOI":"10.1007\/978-981-15-8229-5_3"},{"key":"30","unstructured":"Sudo, S., and Yamamoto, A. (2017) Three-component simultaneous anal\u00adysis device and three-component simultaneous anal\u00adysis method. Japan Patent WO2017138646A1, World Intellectual Property Organization. URL https:\/\/patents.google.com\/patent\/WO2017138646A1\/ja"},{"key":"31","doi-asserted-by":"crossref","unstructured":"Takahashi, M., Shimada, S., Nakayama, N., and Arihara, J. (2005) Characteristics of nodulation and nitrogen fixation in the improved supernodulating soybean (<i>Glycine max<\/i> L. Merr.) cultivar \u2018Sakukei 4\u2019. Plant Prod Sci               8: 405\u2013411.","DOI":"10.1626\/pps.8.405"},{"key":"32","doi-asserted-by":"crossref","unstructured":"Takata, Y., Kawahigashi, M., Kida, K., Tani, M., Kinoshita, R., Ito, T., et al. (2021) Major soil types. In <i>The Soils of Japan<\/i>. Hatano, R., Shinjo, H., and Takata, Y. (eds). Singapore: Springer Singapore, pp. 69\u2013132.","DOI":"10.1007\/978-981-15-8229-5_4"},{"key":"33","doi-asserted-by":"crossref","unstructured":"Takeguchi, Y., Shibuya, R., Kondo, M., Betsuyaku, E., Itakura, M., Minamisawa, K., et al. (2025) Unipolar polysaccharide-mediated attachment of the N<sub>2<\/sub>O-reducing bacterium <i>Bradyrhizobium ottawaense<\/i> SG09 to plant roots. Microbes Environ               40: ME25043.","DOI":"10.1264\/jsme2.ME25043"},{"key":"34","doi-asserted-by":"crossref","unstructured":"Tian, H., Pan, N., Thompson, R.L., Canadell, J.G., Suntharalingam, P., Regnier, P., et al. (2024) Global nitrous oxide budget (1980\u20132020). Earth Syst Sci Data               16: 2543\u20132604.","DOI":"10.5194\/essd-16-2543-2024"},{"key":"35","doi-asserted-by":"crossref","unstructured":"Toyoda, S., Damak, F., Hattori, S., Takeda, M., Akiyama, H., Sasaki, Y., and Minamisawa, K. (2024) Dynamics of N<sub>2<\/sub>O production and reduction processes in a soybean field revealed by isotopocule anal\u00adyses. Soil Biol Biochem               191: 109358.","DOI":"10.1016\/j.soilbio.2024.109358"},{"key":"36","doi-asserted-by":"crossref","unstructured":"Uchida, Y., and Akiyama, H. (2013) Mitigation of postharvest nitrous oxide emissions from soybean ecosystems: A review. Soil Sci Plant Nutr               59: 477\u2013487.","DOI":"10.1080\/00380768.2013.805433"},{"key":"37","doi-asserted-by":"crossref","unstructured":"Usyskin-Tonne, A., Hadar, Y., and Minz, D. (2019) Altering N<sub>2<\/sub>O emissions by manipulating wheat root bacterial community. Sci Rep               9: 7613.","DOI":"10.1038\/s41598-019-44124-3"},{"key":"38","doi-asserted-by":"crossref","unstructured":"Wang, C., Amon, B., Schulz, K., and Mehdi, B. (2021a) Factors that influence nitrous oxide emissions from agricultural soils as well as their representation in simulation models: A review. Agronomy (Basel, Switz)               11: 770.","DOI":"10.3390\/agronomy11040770"},{"key":"39","doi-asserted-by":"crossref","unstructured":"Wang, T., Guo, J., Peng, Y., Lyu, X., Liu, B., Sun, S., and Wang, X. (2021b) Light-induced mobile factors from shoots regulate rhizobium-triggered soybean root nodulation. Science               374: 65\u201371.","DOI":"10.1126\/science.abh2890"},{"key":"40","doi-asserted-by":"crossref","unstructured":"Wasai-Hara, S., Hara, S., Morikawa, T., Sugawara, M., Takami, H., Yoneda, J., et al. (2020) Diversity of <i>Bradyrhizobium<\/i> in non-leguminous sorghum plants: <i>B. ottawaense<\/i> isolates unique in genes for N<sub>2<\/sub>O reductase and lack of the type VI secretion system. Microbes Environ               35: ME19102.","DOI":"10.1264\/jsme2.ME19102"},{"key":"41","doi-asserted-by":"crossref","unstructured":"Wasai-Hara, S., Itakura, M., Fernandes Siqueira, A., Takemoto, D., Sugawara, M., Mitsui, H., et al. (2023) <i>Bradyrhizobium ottawaense<\/i> efficiently reduces nitrous oxide through high <i>nosZ<\/i> gene expression. Sci Rep               13: 18862.","DOI":"10.1038\/s41598-023-46019-w"},{"key":"42","doi-asserted-by":"crossref","unstructured":"Weil, R.R., and Ohlrogge, A.J. (1975) Seasonal development of, and the effect of inter-plant competition on, soybean nodules. Agron J               67: 487\u2013490.","DOI":"10.2134\/agronj1975.00021962006700040009x"},{"key":"43","doi-asserted-by":"crossref","unstructured":"Win, K.T., Wasai-Hara, S., Tanaka, F., Oo, A.Z., Minamisawa, K., Shimoda, Y., and Imaizumi-Anraku, H. (2023) Synergistic N<sub>2<\/sub>-fixation and salt stress mitigation in soybean through dual inoculation of ACC deaminase-producing <i>Pseudomonas<\/i> and <i>Bradyrhizobium<\/i>. Sci Rep               13: 17050.","DOI":"10.1038\/s41598-023-43891-4"},{"key":"44","doi-asserted-by":"publisher","unstructured":"Win, K.T., Tanaka, F., Minamisawa, K., and Imaizumi-Anraku, H. (2024) Growth and yield dynamics in three Japanese soybean cultivars with plant growth-promoting <i>Pseudomonas<\/i> spp. and <i>Bradyrhizobium ottawaense<\/i> co-inoculation. Microorganisms               12: 1478.","DOI":"10.3390\/microorganisms12071478"},{"key":"45","unstructured":"World Meteorological Organization (WMO). (2022) <i>Scientific Assessment of Ozone Depletion: 2022<\/i>. GAW Report No. 278.               Geneva: WMO."},{"key":"46","doi-asserted-by":"crossref","unstructured":"Wrage, N., Velthof, G.L., Van Beusichem, M.L., and Oenema, O. (2001) Role of nitrifier denitrification in the production of nitrous oxide. Soil Biol Biochem               33: 1723\u20131732.","DOI":"10.1016\/S0038-0717(01)00096-7"},{"key":"47","doi-asserted-by":"crossref","unstructured":"Xia, F., Mei, K., Xu, Y., Zhang, C., Dahlgren, R.A., and Zhang, M. (2020) Response of N<sub>2<\/sub>O emission to manure application in field trials of agricultural soils across the globe. Sci Total Environ               733: 139390.","DOI":"10.1016\/j.scitotenv.2020.139390"},{"key":"48","doi-asserted-by":"crossref","unstructured":"Yang, L., and Cai, Z. (2005) The effect of growing soybean (<i>Glycine max<\/i> L.) on N<sub>2<\/sub>O emission from soil. Soil Biol Biochem               37: 1205\u20131209.","DOI":"10.1016\/j.soilbio.2004.08.027"},{"key":"49","doi-asserted-by":"crossref","unstructured":"Yoon, S., Song, B., Phillips, R.L., Chang, J., and Song, M.J. (2019) Ecological and physiological implications of nitrogen oxide reduction pathways on greenhouse gas emissions in agroecosystems. FEMS Microbiol Ecol               95: fiz066.","DOI":"10.1093\/femsec\/fiz066"},{"key":"50","doi-asserted-by":"crossref","unstructured":"Zumft, W.G. (1997) Cell biology and molecular basis of denitrification. Microbiol Mol Biol Rev               61: 533\u2013616.","DOI":"10.1128\/mmbr.61.4.533-616.1997"}],"container-title":["Microbes and Environments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jsme2\/41\/3\/41_ME26022\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:54:56Z","timestamp":1789188896000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jsme2\/41\/3\/41_ME26022\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":50,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.1264\/jsme2.me26022","relation":{},"ISSN":["1342-6311","1347-4405"],"issn-type":[{"value":"1342-6311","type":"print"},{"value":"1347-4405","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"ME26022"}}