{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T11:46:25Z","timestamp":1785325585444,"version":"3.55.0"},"reference-count":0,"publisher":"Pakistan Journal of Medical Sciences","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pak J Med Sci"],"abstract":"<jats:p>Objective: To correlate the results of HER2\/neu protein overexpression on immunohistochemistry (IHC) and gene amplification on fluorescence in situ hybridization (FISH) and to document the problems faced in performing FISH procedure.\r\nMethods: This was an observational retrospective study covering five years from January 1st, 2015 - December 31st, 2019 at Histopathology Department of Shifa International Hospital (SIH), Islamabad. All cases of breast cancer that underwent florescence in situ hybridization (FISH) were retrieved. Correlation between HER2\/neu overexpression on IHC and its amplification on FISH was analyzed. Problems in application of FISH were recorded.\r\nResults: Out of 451 cases submitted for HER2\/neu testing by FISH, 68 cases (15%) were rejected. Gene amplification was seen in 139 (36.29%) cases. Total cases with HER2\/neu IHC score of 2+ were 330 cases and out of which gene amplification was seen in 98 cases (29.69%) whereas 93.1% (41\/44) 3+ IHC cases were amplified. Poor fixation, inadequate amount of tumor with crushing artefacts and dye application to the biopsy fragments were causes of sample rejection.\r\nConclusions: Her2\/neu amplification was seen in most Her2\/neu 3+ cases and approximately one-third of Her2neu 2+ cases. Proper fixation, adequate biopsy material with standardized processing is required to yield useful results on FISH.\r\ndoi: https:\/\/doi.org\/10.12669\/pjms.39.6.6704\r\nHow to cite this: Mukhtar Z, Faisal A, Mudassir G, Mamoon N. Correlation between HER2\/neu protein overexpression on Immunohistochemistry and Fluorescent in Situ Hybridization (FISH) in breast carcinoma: Problems in developing countries. Pak J Med Sci. 2023;39(6):1814-1817.\u00a0 doi: https:\/\/doi.org\/10.12669\/pjms.39.6.6704\r\nThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http:\/\/creativecommons.org\/licenses\/by\/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.<\/jats:p>","DOI":"10.12669\/pjms.39.6.6704","type":"journal-article","created":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T16:51:15Z","timestamp":1694623875000},"source":"Crossref","is-referenced-by-count":2,"title":["Correlation between HER2\/neu protein overexpression on Immunohistochemistry and Fluorescent in Situ Hybridization (FISH) in breast carcinoma: Problems in developing countries"],"prefix":"10.12669","volume":"39","author":[{"family":"Zubaria Mukhtar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Amina Faisal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghazala","family":"Mudassir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Nadira Mamoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4935","published-online":{"date-parts":[[2023,9,23]]},"container-title":["Pakistan Journal of Medical Sciences"],"original-title":[],"link":[{"URL":"https:\/\/pjms.org.pk\/index.php\/pjms\/article\/download\/6704\/1925","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pjms.org.pk\/index.php\/pjms\/article\/download\/6704\/1925","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T09:49:10Z","timestamp":1778320150000},"score":1,"resource":{"primary":{"URL":"https:\/\/pjms.org.pk\/index.php\/pjms\/article\/view\/6704"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,23]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,9,8]]}},"URL":"https:\/\/doi.org\/10.12669\/pjms.39.6.6704","relation":{},"ISSN":["1681-715X","1682-024X"],"issn-type":[{"value":"1681-715X","type":"electronic"},{"value":"1682-024X","type":"print"}],"subject":[],"published":{"date-parts":[[2023,9,23]]}}}