{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:07:20Z","timestamp":1772168840866,"version":"3.50.1"},"update-to":[{"DOI":"10.12688\/f1000research.125272.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000}}],"reference-count":51,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["CA211187"],"award-info":[{"award-number":["CA211187"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background:<\/ns4:bold>\n                    Disruption of natural light cycles, as experienced by shift workers, is linked to enhanced cancer incidence. Several mouse models of cancer develop more severe disease when exposed to irregular light\/dark cycles, supporting the connection between circadian disruption and increased cancer risk. Cryptochrome 2 (CRY2), a repressive component of the molecular circadian clock, facilitates turnover of the oncoprotein c-MYC, one mechanism that may link the molecular clock to tumorigenesis. In E\u03bc-MYC mice, which express transgenic\n                    <ns4:italic>c-MYC<\/ns4:italic>\n                    in B cells and develop aggressive lymphomas and leukemia, global\n                    <ns4:italic>Cry2<\/ns4:italic>\n                    deletion reduces survival and enhances tumor formation. Lighting conditions that mimic the disruption experienced by shift workers dampen\n                    <ns4:italic>Cry2<\/ns4:italic>\n                    transcripts in peripheral tissues of C57BL\/6J mice. Although it is milder than homozygous deletion of\n                    <ns4:italic>Cry2<\/ns4:italic>\n                    , we hypothesized that reduced\n                    <ns4:italic>Cry2<\/ns4:italic>\n                    rhythmicity could alter MYC protein accumulation and contribute to enhanced cancer risk caused by circadian disruption. We tested this hypothesis in MYC-driven lymphoma.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    We housed E\u03bc-MYC mice in light-tight boxes set to either control (continuous cycles of 12-hours of light followed by 12-hours of dark, LD12:12) or chronic jetlag (eight-hour light phase advances every two to three days, CJL) lighting conditions and assessed the impact of disrupted light cycles on survival and tumor formation in E\u03bc-MYC mice.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results:<\/ns4:bold>\n                    Environmental disruption of circadian rhythms did not alter tumor location, tumor growth, or survival in E\u03bc-MYC mice.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusions:<\/ns4:bold>\n                    Dampened rhythms of\n                    <ns4:italic>Cry2<\/ns4:italic>\n                    following disruption of circadian light exposures is milder than deletion of\n                    <ns4:italic>Cry2<\/ns4:italic>\n                    . The lack of phenotype caused by altered circadian gene expression in contrast to enhanced tumorigenesis caused by homozygous deletion of\n                    <ns4:italic>Cry2<\/ns4:italic>\n                    suggests that CRY2 dosage impacts this model. Importantly, these findings indicate that increased cancer risk associated with circadian disruption arises from one or more mechanisms that are not recapitulated here, and may be different in distinct tumor types.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.125272.2","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T11:55:30Z","timestamp":1695902130000},"page":"49","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Circadian disruption does not alter tumorigenesis in a mouse model of lymphoma"],"prefix":"10.12688","volume":"12","author":[{"given":"Rebecca M","family":"Mello","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2325-6394","authenticated-orcid":false,"given":"Marie","family":"Pariollaud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9533-0499","authenticated-orcid":false,"given":"Katja A","family":"Lamia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2023,9,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"935-941","DOI":"10.1038\/nature00965","article-title":"Coordination of circadian timing in mammals.","volume":"418","author":"S Reppert","year":"2002","journal-title":"Nature."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"994-999","DOI":"10.1126\/science.aah4965","article-title":"Circadian time signatures of fitness and disease.","volume":"354","author":"J Bass","year":"2016","journal-title":"Science (New York, N.Y.)."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"2148-2151","DOI":"10.1002\/ijc.23566","article-title":"Night-time work predisposes to non-Hodgkin lymphoma.","volume":"123","author":"T Lahti","year":"2008","journal-title":"Int. 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