{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T09:39:00Z","timestamp":1767173940937,"version":"build-2238731810"},"update-to":[{"DOI":"10.12688\/f1000research.128978.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T00:00:00Z","timestamp":1692316800000}},{"DOI":"10.12688\/f1000research.128978.2","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T00:00:00Z","timestamp":1692316800000}}],"reference-count":76,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T00:00:00Z","timestamp":1692316800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009736","name":"Freiwillige Akademische Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009736","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Introduction:<\/ns4:bold>\n                    Growing scientific evidence indicates that sphingolipids predict cardiometabolic risk, independently of and beyond traditional biomarkers such as low-density lipoprotein cholesterol. To date, it remains largely unknown if and how exercise, a simple, low-cost, and patient-empowering modality to optimise cardiometabolic health, influences sphingolipid levels. The SphingoHIIT study aims to assess the response of circulating sphingolipid species to a single session of high-intensity interval training (HIIT).\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    This single-centre randomised controlled trial (RCT) will last 11 days per participant and aim to include 32 young and healthy individuals aged 20-29 (50% females). Participants will be randomly allocated to the HIIT (n= 16) or control groups (physical rest, n= 16). Participants will self-sample fasted dried blood spots for three consecutive days before the intervention (HIIT versus rest) to determine baseline sphingolipid levels. Dried blood spots will also be collected at five time points (2, 15, 30, 60min, and 24h) following the intervention (HIIT versus rest). To minimise the dietary influence, participants will receive a standardised diet for four days, starting 24 hours before the first dried blood sampling. For females, interventions will be timed to fall within the early follicular phase to minimise the menstrual cycle's influence on sphingolipid levels. Finally, physical activity will be monitored for the whole study duration using a wrist accelerometer.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Ethics and dissemination:<\/ns4:bold>\n                    The Ethics Committee of Northwest and Central Switzerland approved this protocol (ID 2022\u201300513). Findings will be disseminated in scientific journals and meetings.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Trial Registration<\/ns4:bold>\n                    The trial was registered on www.clinicaltrials.gov (NCT05390866,\n                    <ns4:ext-link xmlns:ns5=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" ns5:href=\"https:\/\/clinicaltrials.gov\/ct2\/show\/NCT05390866\">https:\/\/clinicaltrials.gov\/ct2\/show\/NCT05390866<\/ns4:ext-link>\n                    ) on May 25, 2022.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.128978.3","type":"journal-article","created":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T06:45:24Z","timestamp":1692341124000},"page":"1565","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigating the circulating sphingolipidome response to a single high-intensity interval training session within healthy females and males in their twenties (SphingoHIIT): Protocol for a randomised controlled trial"],"prefix":"10.12688","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2380-105X","authenticated-orcid":false,"given":"Justin","family":"Carrard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Angst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadia","family":"Weber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00eblle","family":"Bienvenue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Infanger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukas","family":"Streese","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timo","family":"Hinrichs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilaria","family":"Croci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Schmied","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hector","family":"Gallart-Ayala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"H\u00f6chsmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9618-2069","authenticated-orcid":false,"given":"Karsten","family":"Koehler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henner","family":"Hanssen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julijana","family":"Ivanisevic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arno","family":"Schmidt-Trucks\u00e4ss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2023,8,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"2095-2128","DOI":"10.1016\/S0140-6736(12)61728-0","article-title":"Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010.","volume":"380","author":"R Lozano","year":"2012","journal-title":"Lancet."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"393-397","DOI":"10.1016\/j.gheart.2016.10.024","article-title":"The Global Burden of Disease Study and the Preventable Burden of NCD.","volume":"11","author":"C Benziger","year":"2016","journal-title":"Glob. Heart."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"111-188","DOI":"10.1093\/eurheartj\/ehz455","article-title":"2019 ESC\/EAS Guidelines for the management of dyslipidaemias: lipid modification to reduce cardiovascular risk: The Task Force for the management of dyslipidaemias of the European Society of Cardiology (ESC) and European Atherosclerosis Society (EAS).","volume":"41","author":"F Mach","year":"2019","journal-title":"Eur. Heart J."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"83-108","DOI":"10.1016\/j.plipres.2015.12.002","article-title":"Novel advances in shotgun lipidomics for biology and medicine.","volume":"61","author":"M Wang","year":"2016","journal-title":"Prog. 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Chem."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"2588-2597","DOI":"10.2337\/db10-1221","article-title":"Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: another paradox in endurance-trained athletes?","volume":"60","author":"F Amati","year":"2011","journal-title":"Diabetes."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"1082-1095","DOI":"10.1016\/j.tips.2021.10.001","article-title":"Cholesterol \u2013 the devil you know; ceramide \u2013 the devil you don\u2019t.","volume":"42","author":"T Tippetts","year":"2021","journal-title":"Trends Pharmacol. Sci."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"1451-1462","DOI":"10.1136\/bjsports-2020-102955","article-title":"World Health Organization 2020 guidelines on physical activity and sedentary behaviour.","volume":"54","author":"F Bull","year":"2020","journal-title":"Br. J. Sports Med."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"59-64","DOI":"10.1016\/S0140-6736(12)60865-4","article-title":"Sport and exercise as contributors to the health of nations.","volume":"380","author":"K Khan","year":"2012","journal-title":"Lancet."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"294-305","DOI":"10.1016\/S0140-6736(12)60898-8","article-title":"The pandemic of physical inactivity: global action for public health.","volume":"380","author":"H Kohl","year":"2012","journal-title":"Lancet."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"17-96","DOI":"10.1093\/eurheartj\/ehaa605","article-title":"2020 ESC Guidelines on sports cardiology and exercise in patients with cardiovascular disease.","volume":"42","author":"A Pelliccia","year":"2021","journal-title":"Eur. Heart J."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"731-743","DOI":"10.1038\/s41569-018-0065-1","article-title":"Exercise benefits in cardiovascular disease: beyond attenuation of traditional risk factors.","volume":"15","author":"C Fiuza-Luces","year":"2018","journal-title":"Nat. Rev. Cardiol."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"255-323","DOI":"10.1093\/eurheartj\/ehz486","article-title":"2019 ESC Guidelines on diabetes, pre-diabetes, and cardiovascular diseases developed in collaboration with the EASD.","volume":"41","author":"F Cosentino","year":"2020","journal-title":"Eur. Heart J."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"545-555","DOI":"10.1038\/s41574-020-0381-5","article-title":"Diet and exercise in the prevention and treatment of type 2 diabetes mellitus.","volume":"16","author":"F Magkos","year":"2020","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"e001008","DOI":"10.1136\/bmjsem-2020-001008","article-title":"The Metabolic Signature of Cardiorespiratory Fitness: A Systematic Review.","volume":"7","author":"J Carrard","year":"2021","journal-title":"Sports Med."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"E398-E408","DOI":"10.1152\/ajpendo.00134.2015","article-title":"Serum sphingolipids: relationships to insulin sensitivity and changes with exercise in humans.","volume":"309","author":"B Bergman","year":"2015","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"1414-1421","DOI":"10.1002\/oby.21117","article-title":"Improved insulin sensitivity after exercise training is linked to reduced plasma C14:0 ceramide in obesity and type 2 diabetes.","volume":"23","author":"T Kasumov","year":"2015","journal-title":"Obesity (Silver Spring)."},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01550","article-title":"Inflammatory Effects of High and Moderate Intensity Exercise\u2014A Systematic Review.","volume":"10","author":"E Cerqueira","year":"2020","journal-title":"Front. Physiol."},{"key":"ref75","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.117.007931","article-title":"Ceramide Remodeling and Risk of Cardiovascular Events and Mortality.","volume":"7","author":"L Peterson","year":"2018","journal-title":"J. Am. Heart Assoc."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"e009305","DOI":"10.1161\/JAHA.118.009305","article-title":"High-Intensity Interval Training for Patients With Cardiovascular Disease-Is It Safe? A Systematic Review.","volume":"7","author":"M Wewege","year":"2018","journal-title":"J. Am. Heart Assoc."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"140-146","DOI":"10.1016\/j.pcad.2019.01.004","article-title":"Guidelines for the delivery and monitoring of high intensity interval training in clinical populations.","volume":"62","author":"J Taylor","year":"2019","journal-title":"Prog. Cardiovasc. Dis."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"1227-1234","DOI":"10.1136\/bjsports-2013-092576","article-title":"High-intensity interval training in patients with lifestyle-induced cardiometabolic disease: a systematic review and meta-analysis.","volume":"48","author":"K Weston","year":"2014","journal-title":"Br. J. Sports Med."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"e000143","DOI":"10.1136\/bmjsem-2016-000143","article-title":"Update on the effects of physical activity on insulin sensitivity in humans.","volume":"2","author":"S Bird","year":"2017","journal-title":"BMJ Open Sport Exercise Medicine."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"1220-1226","DOI":"10.1249\/MSS.0000000000001934","article-title":"High-Intensity Interval Training for Cardiometabolic Disease Prevention.","volume":"51","author":"W Campbell","year":"2019","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref76","doi-asserted-by":"publisher","first-page":"692-701","DOI":"10.1177\/2047487319887828","article-title":"High-intensity interval training has a greater effect on reverse cholesterol transport elements compared with moderate-intensity continuous training in obese male rats.","volume":"28","author":"S Rahmati-Ahmadabad","year":"2021","journal-title":"Eur. J. Prev. Cardiol."},{"key":"ref77","doi-asserted-by":"publisher","first-page":"1513-1523","DOI":"10.1007\/s00421-019-04138-1","article-title":"Acute cardiopulmonary responses to strength training, high-intensity interval training and moderate-intensity continuous training.","volume":"119","author":"R Falz","year":"2019","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"14468-14473","DOI":"10.1073\/pnas.1222647110","article-title":"Extensive diversity in circadian regulation of plasma lipids and evidence for different circadian metabolic phenotypes in humans.","volume":"110","author":"E Chua","year":"2013","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"440-450","DOI":"10.1017\/S0029665116000288","article-title":"Circadian regulation of lipid metabolism.","volume":"75","author":"J Gooley","year":"2016","journal-title":"Proc. Nutr. Soc."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"4973-4993","DOI":"10.1007\/s00216-015-8633-2","article-title":"Optimizing the lipidomics workflow for clinical studies\u2014practical considerations.","volume":"407","author":"T Hy\u00f6tyl\u00e4inen","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"1112-30.e16","DOI":"10.1016\/j.cell.2020.04.043","article-title":"Molecular Choreography of Acute Exercise.","volume":"181","author":"K Contrepois","year":"2020","journal-title":"Cell."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"1905-1924","DOI":"10.1161\/CIRCULATIONAHA.120.050281","article-title":"Metabolic Architecture of Acute Exercise Response in Middle-Aged Adults in the Community.","volume":"142","author":"M Nayor","year":"2020","journal-title":"Circulation."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"273-289","DOI":"10.1038\/s41574-022-00641-2","article-title":"Exerkines in health, resilience and disease.","volume":"18","author":"L Chow","year":"2022","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"eaar5551","DOI":"10.1126\/science.aar5551","article-title":"Sphingosine 1-phosphate: Lipid signaling in pathology and therapy.","volume":"366","author":"A Cartier","year":"2019","journal-title":"Science."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"e7586","DOI":"10.1136\/bmj.e7586","article-title":"SPIRIT 2013 explanation and elaboration: guidance for protocols of clinical trials.","volume":"346","author":"A Chan","year":"2013","journal-title":"BMJ: Br. Med. J."},{"key":"ref53","first-page":"3-17","article-title":"The physical activity readiness questionnaire for everyone (PAR-Q+) and electronic physical activity readiness medical examination (ePARmed-X+).","volume":"4","author":"D Warburton","year":"2011","journal-title":"The Health & Fitness Journal of Canada."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"834-851","DOI":"10.1176\/appi.ajp.2013.12060782","article-title":"DSM-5 criteria for substance use disorders: recommendations and rationale.","volume":"170","author":"D Hasin","year":"2013","journal-title":"Am. J. Psychiatry."},{"key":"ref55","article-title":"Division of Population Health, National Center for Chronic Disease Prevention and Health Promotion, Prevention CfDCa. Alcohol Use and Your Health: Centers for Disease Control and Prevention.","year":"2021"},{"key":"ref56","doi-asserted-by":"publisher","first-page":"2473-2479","DOI":"10.1249\/MSS.0000000000000664","article-title":"Updating ACSM\u2019s Recommendations for Exercise Preparticipation Health Screening.","volume":"47","author":"D Riebe","year":"2015","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref57","first-page":"15-20","article-title":"Blocked randomization with randomly selected block sizes.","volume":"8","author":"J Efird","year":"2011","journal-title":"Int. J. Environ. Res. Public Health."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"479-485","DOI":"10.1016\/j.nutres.2012.05.009","article-title":"Utility of multifrequency bioelectrical impedance compared with dual-energy x-ray absorptiometry for assessment of total and regional body composition varies between men and women.","volume":"32","author":"L Anderson","year":"2012","journal-title":"Nutr. Res."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1186\/s12891-015-0510-9","article-title":"Concordance between muscle mass assessed by bioelectrical impedance analysis and by dual energy X-ray absorptiometry: a cross-sectional study.","volume":"16","author":"F Buckinx","year":"2015","journal-title":"BMC Musculoskelet. Disord."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"S49-S55","DOI":"10.1164\/arrd.1984.129.2P2.S49","article-title":"Predicted values for clinical exercise testing.","volume":"129","author":"J Hansen","year":"1984","journal-title":"Am. Rev. Respir. Dis."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"147-155","DOI":"10.1007\/s00421-012-2421-x","article-title":"Associations between Borg\u2019s rating of perceived exertion and physiological measures of exercise intensity.","volume":"113","author":"J Scherr","year":"2013","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"67-77","DOI":"10.1016\/j.pcad.2017.03.006","article-title":"High Intensity Interval Training for Maximizing Health Outcomes.","volume":"60","author":"T Karlsen","year":"2017","journal-title":"Prog. Cardiovasc. Dis."},{"key":"ref63","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00019","article-title":"A Multi-Center Comparison of O2peak Trainability Between Interval Training and Moderate Intensity Continuous Training.","volume":"10","author":"C Williams","year":"2019","journal-title":"Front. Physiol."},{"key":"ref64","doi-asserted-by":"publisher","first-page":"241-247","DOI":"10.1093\/ajcn\/51.2.241","article-title":"A new predictive equation for resting energy expenditure in healthy individuals.","volume":"51","author":"M Mifflin","year":"1990","journal-title":"Am. J. Clin. Nutr."},{"key":"ref65","doi-asserted-by":"publisher","first-page":"826-837","DOI":"10.1016\/S0140-6736(11)60812-X","article-title":"Quantification of the effect of energy imbalance on bodyweight.","volume":"378","author":"K Hall","year":"2011","journal-title":"Lancet."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"66-74","DOI":"10.3945\/ajcn.111.014399","article-title":"Estimating changes in free-living energy intake and its confidence interval.","volume":"94","author":"K Hall","year":"2011","journal-title":"Am. J. Clin. Nutr."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"104895","DOI":"10.1016\/j.psyneuen.2020.104895","article-title":"How to study the menstrual cycle: Practical tools and recommendations.","volume":"123","author":"K Schmalenberger","year":"2021","journal-title":"Psychoneuroendocrinology."},{"key":"ref68","doi-asserted-by":"publisher","first-page":"843-861","DOI":"10.1007\/s40279-021-01435-8","article-title":"Methodological Considerations for Studies in Sport and Exercise Science with Women as Participants: A Working Guide for Standards of Practice for Research on Women.","volume":"51","author":"K Elliott-Sale","year":"2021","journal-title":"Sports Med."},{"key":"ref69","article-title":"A crash course in good and bad controls. Available at SSRN 3689437.","author":"C Cinelli","year":"2020"},{"key":"ref74","doi-asserted-by":"publisher","DOI":"10.17605\/OSF.IO\/53QND","article-title":"Investigating the circulating sphingolipidome response to a single high-intensity interval training session (SphingoHIIT): Protocol for a randomised controlled trial.","author":"J Carrard","year":"2022, November 27"},{"key":"ref70","first-page":"1887-1894","article-title":"Robust causal inference using directed acyclic graphs: the R package \u2018dagitty\u2019.","volume":"45","author":"J Textor","year":"2017","journal-title":"Int. J. Epidemiol."},{"key":"ref71","doi-asserted-by":"publisher","first-page":"289-300","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing.","volume":"57","author":"Y Benjamini","year":"1995","journal-title":"Journal of the Royal Statistical Society Series B (Methodological)."},{"key":"ref72","doi-asserted-by":"publisher","first-page":"c332","DOI":"10.1136\/bmj.c332","article-title":"CONSORT 2010 Statement: updated guidelines for reporting parallel group randomised trials.","volume":"340","author":"K Schulz","year":"2010","journal-title":"BMJ."},{"key":"ref73","article-title":"Clinical safety data management: definitions and standards for expedited reporting E2A. 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These foundations were not involved in the study conception or manuscript writing. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}