{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:07:32Z","timestamp":1772168852640,"version":"3.50.1"},"reference-count":21,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007698","name":"University of Florida","doi-asserted-by":"publisher","award":["GlobalFellowsProgramAward"],"award-info":[{"award-number":["GlobalFellowsProgramAward"]}],"id":[{"id":"10.13039\/100007698","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013702","name":"College of Agricultural and Life Sciences, University of Florida","doi-asserted-by":"publisher","award":["Dean\u2019sAward"],"award-info":[{"award-number":["Dean\u2019sAward"]}],"id":[{"id":"10.13039\/100013702","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013702","name":"College of Agricultural and Life Sciences, University of Florida","doi-asserted-by":"publisher","award":["MatchingAssistantship"],"award-info":[{"award-number":["MatchingAssistantship"]}],"id":[{"id":"10.13039\/100013702","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015283","name":"Bloomberg Philanthropies","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015283","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["2U19AI089680"],"award-info":[{"award-number":["2U19AI089680"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32AI-138953"],"award-info":[{"award-number":["T32AI-138953"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011780","name":"Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>\n                    Despite efforts to minimize the impacts of malaria and reduce the number of primary vectors, malaria has yet to be eliminated in Zambia. Understudied vector species may perpetuate malaria transmission in pre-elimination settings.\n                    <ns3:italic>Anopheles squamosus<\/ns3:italic>\n                    is one of the most abundantly caught mosquito species in southern Zambia and has previously been found with\n                    <ns3:italic>Plasmodium falciparum<\/ns3:italic>\n                    sporozoites, a causal agent of human malaria. This species may be a critical vector of malaria transmission, however, there is a lack of genetic information available for\n                    <ns3:italic>An. squamosus<\/ns3:italic>\n                    . We report the first genome data and the first complete mitogenome (Mt) sequence of\n                    <ns3:italic>An. squamosus<\/ns3:italic>\n                    . The sequence was extracted from one individual mosquito from the Chidakwa area in Macha, Zambia. The raw reads were obtained using Illumina Novaseq 6000 and assembled through NOVOplasty alignment with related species. The length of the\n                    <ns3:italic>An. squamosus<\/ns3:italic>\n                    Mt was 15,351 bp, with 77.9 % AT content. The closest match to the whole mitochondrial genome in the phylogenetic tree is the African malaria mosquito,\n                    <ns3:italic>Anopheles gambiae<\/ns3:italic>\n                    . Its genome data is available through National Center for Biotechnology Information (NCBI) Sequencing Reads Archive (SRA) with accession number\n                    <ns3:ext-link xmlns:ns4=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" ns4:href=\"https:\/\/identifiers.org\/insdc.sra:SRR22114392\">SRR22114392<\/ns3:ext-link>\n                    . The mitochondrial genome was deposited in NCBI GenBank with the accession number\n                    <ns3:ext-link xmlns:ns4=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" ns4:href=\"https:\/\/identifiers.org\/ncbi\/insdc:OP776919\">OP776919<\/ns3:ext-link>\n                    . The ITS2 containing contig sequence was deposited in GenBank with the accession number\n                    <ns3:ext-link xmlns:ns4=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" ns4:href=\"https:\/\/identifiers.org\/ncbi\/insdc:OQ241725\">OQ241725<\/ns3:ext-link>\n                    . Mitogenome annotation and a phylogenetic tree with related\n                    <ns3:italic>Anopheles<\/ns3:italic>\n                    mosquito species are provided.\n                  <\/ns3:p>","DOI":"10.12688\/f1000research.130734.1","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T08:40:14Z","timestamp":1679647214000},"page":"330","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["The first genome sequence of Anopheles squamous from Macha, Zambia"],"prefix":"10.12688","volume":"12","author":[{"given":"Valerie T.","family":"Nguyen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Travis C.","family":"Collier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangwoo","family":"Seok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monicah M.","family":"Mburu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Limonty","family":"Simubali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary E.","family":"Gebhardt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4631-2777","authenticated-orcid":false,"given":"Douglas E.","family":"Norris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoosook","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"key":"ref2","doi-asserted-by":"publisher","first-page":"1482-1487","DOI":"10.1093\/jme\/tjw091","article-title":"Detection of Plasmodium falciparum Infection in Anopheles squamosus (Diptera: Culicidae) in an Area Targeted for Malaria Elimination, Southern Zambia.","volume":"53","author":"J Stevenson","year":"2016 Nov","journal-title":"J. Med. Entomol."},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3390\/insects12020146","article-title":"Phylogenetic Complexity of Morphologically Identified Anopheles squamosus in Southern Zambia.","volume":"12","author":"J Hoffman","year":"2021 Feb 8","journal-title":"Insects."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1173-1179","DOI":"10.1089\/vbz.2010.0082","article-title":"Unexpected anthropophily in the potential secondary malaria vectors Anopheles coustani s.l. and Anopheles squamosus in Macha, Zambia.","volume":"11","author":"C Fornadel","year":"2011 Aug","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref5","first-page":"1-18","article-title":"Implicating cryptic and novel anophelines as malaria vectors in Africa.","volume":"8","author":"J Stevenson","year":"2017","journal-title":"Insects."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"5145-5157","DOI":"10.1111\/mec.13382","article-title":"Complex genome evolution in Anopheles coluzzii associated with increased insecticide usage in Mali.","volume":"24","author":"B Main","year":"2015 Oct","journal-title":"Mol. 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Extensive introgression in a malaria vector species complex revealed by phylogenomics.","volume":"347","author":"M Fontaine","year":"2015 Jan 2","journal-title":"Science."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"14","DOI":"10.3390\/insects14010014","article-title":"Population genetics of Anopheles pretoriensis in Grande Comore Island.","volume":"14","author":"M Campos","year":"2023","journal-title":"Insects."},{"key":"ref21","article-title":"The Mosquitoes of Macha, Zambia.","author":"R Kent","year":"2006 cited 2022 Nov 8","journal-title":"The third International Malaria Research Conference."},{"key":"ref22","article-title":"Anopheles squamosus Theobald.","year":"1901. cited 2022 Nov 3"}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/12-330\/v1\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/12-330\/v1\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/12-330\/v1\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T06:20:46Z","timestamp":1697005246000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/12-330\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,24]]},"references-count":21,"URL":"https:\/\/doi.org\/10.12688\/f1000research.130734.1","relation":{"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.143510.r200578","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.143510.r167805","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.143510.r167805","asserted-by":"object"},{"id-type":"doi","id":"10.5256\/f1000research.143510.r200578","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,24]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/12-330\/v1#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.143510.r167805, Megan A. Riddin, UP Institute for Sustainable Malaria Control (UP ISMC), Faculty of Health Sciences, University of Pretoria, Pretoria, 05 Apr 2023, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/12-330\/v1#referee-response-167805","order":0,"name":"referee-response-167805","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.143510.r200578, Tara Roth, San Mateo County Mosquito and Vector Control District, Burlingame, California, 11 Oct 2023, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/12-330\/v1#referee-response-200578","order":1,"name":"referee-response-200578","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This study was supported by University of Florida College of Agricultural and Life Sciences Dean\u2019s Award to VTN, University of Florida Department of Entomology and Nematology Matching Assistantship to SS, University of Florida International Center Global Fellows Program Award to YL, NIH T32 Training Grant (T32 AI-138953) support to MEG, NIH support through the International Centers of Excellence for Malaria Research (2U19AI089680) for DEN, MEG, MMM and LS, and support to DEN and MEG from the Bloomberg Philanthropies and the Johns Hopkins Malaria Research Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}