{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T16:02:48Z","timestamp":1778860968961,"version":"3.51.4"},"update-to":[{"DOI":"10.12688\/f1000research.131507.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T00:00:00Z","timestamp":1690502400000}}],"reference-count":21,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T00:00:00Z","timestamp":1690502400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["221640"],"award-info":[{"award-number":["221640"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["InstitutionalStrategicSupportFund"],"award-info":[{"award-number":["InstitutionalStrategicSupportFund"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010757","name":"National Centre for the Replacement Refinement and Reduction of Animals in Research","doi-asserted-by":"publisher","award":["NC\/ECA0001\/1"],"award-info":[{"award-number":["NC\/ECA0001\/1"]}],"id":[{"id":"10.13039\/501100010757","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background:<\/ns4:bold>\n                    Sleeping sickness is caused by the extracellular parasite\n                    <ns4:italic>Trypanosoma brucei<\/ns4:italic>\n                    and is associated with neuroinflammation and neuropsychiatric disorders, including disruption of sleep\/wake patterns, and is now recognised as a circadian disorder. Sleeping sickness is traditionally studied using murine models of infection due to the lack of alternative\n                    <ns4:italic>in vitro<\/ns4:italic>\n                    systems that fully recapitulate the cellular diversity and functionality of the human brain. The aim of this study is to develop a much-needed\n                    <ns4:italic>in vitro<\/ns4:italic>\n                    system that reduces and replaces live animals for the study of infections in the central nervous system, using sleeping sickness as a model infection.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    We developed a co-culture system using induced pluripotent stem cell (iPSC)-derived cortical human brain organoids and the human pathogen\n                    <ns4:italic>T. b. gambiense<\/ns4:italic>\n                    to model host-pathogen interactions\n                    <ns4:italic>in vitro<\/ns4:italic>\n                    . Upon co-culture, we analysed the transcriptional responses of the brain organoids to\n                    <ns4:italic>T. b. gambiense<\/ns4:italic>\n                    over two time points.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results:<\/ns4:bold>\n                    We detected broad transcriptional changes in brain organoids exposed to\n                    <ns4:italic>T. b. gambiense<\/ns4:italic>\n                    , mainly associated with innate immune responses, chemotaxis, and blood vessel differentiation compared to untreated organoids.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusions:<\/ns4:bold>\n                    Our co-culture system provides novel, more ethical avenues to study host-pathogen interactions in the brain as alternative models to experimental infections in mice. Although our data support the use of brain organoids to model host-pathogen interactions during\n                    <ns4:italic>T. brucei<\/ns4:italic>\n                    infection as an alternative to\n                    <ns4:italic>in vivo<\/ns4:italic>\n                    models, future work is required to increase the complexity of the organoids (\n                    <ns4:italic>e.g.<\/ns4:italic>\n                    , addition of microglia and vasculature). We envision that the adoption of organoid systems is beneficial to researchers studying mechanisms of brain infection by protozoan parasites. Furthermore, organoid systems have the potential to be used to study other parasites that affect the brain significantly reducing the number of animals undergoing moderate and\/or severe protocols associated with the study of neuroinflammation and brain infections.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.131507.2","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T09:15:06Z","timestamp":1690535706000},"page":"437","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Modelling host-Trypanosoma brucei gambiense interactions in vitro using human induced pluripotent stem cell-derived cortical brain organoids"],"prefix":"10.12688","volume":"12","author":[{"given":"Praveena","family":"Chandrasegaran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agatha","family":"Nabilla Lestari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew C.","family":"Sinton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jay","family":"Gopalakrishnan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5092-5576","authenticated-orcid":false,"given":"Juan F.","family":"Quintana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2023,7,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"951-961.e5","DOI":"10.1016\/j.stem.2020.10.001","article-title":"SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids.","volume":"27","author":"L Pellegrini","year":"2020","journal-title":"Cell Stem Cell."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"e106230","DOI":"10.15252\/embj.2020106230","article-title":"SARS-CoV-2 targets neurons of 3D human brain organoids.","volume":"39","author":"A Ramani","year":"2020","journal-title":"EMBO J."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"952-957","DOI":"10.1242\/dev.140707","article-title":"Using brain organoids to understand Zika virus-induced microcephaly.","volume":"144","author":"X Qian","year":"2017","journal-title":"Dev. 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As requested in the previous round of feedback, we removed Epcam from the list of endothelial markers, not Pecam1. This was a typo and we apologise for this. So, to clarify, we referred to removing all the references to Epcam in the manuscript.","URL":"https:\/\/f1000research.com\/articles\/12-437\/v2#referee-comment-10071","order":2,"name":"referee-comment-10071","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was funded by an NC3Rs Early Career Support Award (NC\/ECA0001\/1 to JFQ), a Wellcome Trust Sir Henry Wellcome postdoctoral fellowship (221640, <a href=https:\/\/doi.org\/10.35802\/221640>https:\/\/doi.org\/10.35802\/221640<\/a>) to JFQ, and Wellcome trust University of Glasgow Institutional Strategic Support Fund (ISSF) feasibility and catalyst grants, all to JFQ.","order":3,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}