{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T23:44:03Z","timestamp":1782258243805,"version":"3.54.5"},"update-to":[{"DOI":"10.12688\/f1000research.132106.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:00:00Z","timestamp":1687305600000}}],"reference-count":41,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:00:00Z","timestamp":1687305600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","award":["80002324"],"award-info":[{"award-number":["80002324"]}],"id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Center of Excellence in Green Materials for Industrial Application","award":["GCE 6508223006-1"],"award-info":[{"award-number":["GCE 6508223006-1"]}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background:<\/ns4:bold>\n                    A significant area of clinical research is the development of natural wound healing products and the management of chronic wounds. Healing wounds with medicinal plants has been a practice of ancient civilizations for centuries.\n                    <ns4:italic>Nigella sativa<\/ns4:italic>\n                    L (\n                    <ns4:italic>N. sativa<\/ns4:italic>\n                    ) is a medicinal plant that has several pharmacological properties.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    The present study evaluated the wound healing properties of\n                    <ns4:italic>Nigella sativa<\/ns4:italic>\n                    L. (\n                    <ns4:italic>N. sativa<\/ns4:italic>\n                    ) seed extracts using normal cell lines such as normal human dermal fibroblasts (NHDFs) and human umbilical vein endothelial cells (HUVECs). The expression levels of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) were analyzed through western blot analysis. Furthermore, computational analyses were carried out to screen the potential bioactive compounds for wound healing applications.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results:<\/ns4:bold>\n                    The results of the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay revealed that, all the tested solvent extracts of\n                    <ns4:italic>N. sativa<\/ns4:italic>\n                    seeds (including ethanol, ethyl acetate, chloroform, and petroleum ether) did not exert any cytotoxic effects at the tested concentrations. Furthermore, the western blot analysis showed elevated levels of VEGF and PDGF upon treatment with\n                    <ns4:italic>N. sativa<\/ns4:italic>\n                    seed extracts. Gas chromatography-mass spectrometry (GC-MS) analysis of\n                    <ns4:italic>N. sativa<\/ns4:italic>\n                    extracts identified 268 phytocompounds. Molecular docking studies revealed that three phytocompounds of\n                    <ns4:italic>N. sativa<\/ns4:italic>\n                    extracts, including tricyclo[20.8.0.0(7,16)]triacontane, 1(22),7(16)-diepoxy-, adaphostin and obeticholic acid had strong binding affinity with wound healing-related target proteins, showing docking scores ranging from -5.5 to -10.9 Kcal\/mol. These compounds had acceptable Absorption, Distribution, Metabolism, and Excretion (ADME) properties.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusions:<\/ns4:bold>\n                    Based on these results,\n                    <ns4:italic>N. sativa<\/ns4:italic>\n                    seed extracts might possess potential wound healing properties owing to the presence of a wide range of bioactive components.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.132106.2","type":"journal-article","created":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T07:20:10Z","timestamp":1687332010000},"page":"436","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Nigella sativa L. seed extracts promote wound healing progress by activating VEGF and PDGF signaling pathways: An in vitro and in silico study"],"prefix":"10.12688","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3527-3477","authenticated-orcid":false,"given":"Chella Perumal","family":"Palanisamy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phaniendra","family":"Alugoju","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selvaraj","family":"Jayaraman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirilux","family":"Poompradub","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2023,6,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"155290","DOI":"10.1016\/j.apsusc.2022.155290","article-title":"Bioactive composite Janus nanofibrous membranes loading Ciprofloxacin and Astaxanthin for enhanced healing of full-thickness skin defect wounds.","volume":"610","author":"W Zhang","year":"2023","journal-title":"Appl. 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Center of Excellence in Green Materials for Industrial Application, Ratchedaphiseksomphot endowment fund (grant no. GCE 6508223006-1) granted to Professor Sirilux Poompradub. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":4,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}