{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:50:58Z","timestamp":1790628658823,"version":"4.1.0"},"update-to":[{"DOI":"10.12688\/f1000research.134956.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000}}],"reference-count":83,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Second Century Fund (C2F) Postdoctoral Fellowship, Chulalongkorn University"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>\n                    Background The Zika virus (ZIKV) infection has emerged as a global health threat. The causal reasoning is that Zika infection is linked to the development of microcephaly in newborns and Guillain-Barr\u00e9 syndrome in adults. With no clinically approved antiviral treatment for ZIKV, the need for the development of potential inhibitors against the virus is essential. In this study, we aimed to screen phytochemicals from papaya (\n                    <ns3:italic>Carica papaya L.<\/ns3:italic>\n                    ) against NS5 protein domains of ZIKV. Methods Approximately 193 phytochemicals from an online database (IMPACT) were subjected to molecular docking using AutoDock Vina against the NS5-MTase protein domain (5WXB) and -RdRp domain (5U04). Results Our results showed that \u03b2-sitosterol, carpaine, violaxanthin, pseudocarpaine, \u03947-avenasterols, Rutin, and cis-\u03b2-carotene had the highest binding affinity to both protein domains, with \u03b2-sitosterol having the most favorable binding energy. Furthermore, ADMET analysis revealed that selected compounds had good pharmacokinetic properties and were nontoxic. Conclusions Our findings suggest that papaya-derived phytochemicals could be potential candidates for developing antiviral drugs against ZIKV. However, further experimental studies using cell lines and\n                    <ns3:italic>in vivo<\/ns3:italic>\n                    models are needed to validate their efficacy and safety.\n                  <\/ns3:p>","DOI":"10.12688\/f1000research.134956.2","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T13:57:07Z","timestamp":1719323827000},"page":"655","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["In silico screening for potential inhibitors from the phytocompounds of Carica papaya against Zika virus NS5 protein"],"prefix":"10.12688","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0693-6366","authenticated-orcid":false,"given":"Kishore Krishna","family":"Kumaree","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naga Venkata Anusha","family":"Anthikapalli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8884-3077","authenticated-orcid":false,"given":"Anchalee","family":"Prasansuklab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2024,6,25]]},"reference":[{"key":"ref3","doi-asserted-by":"publisher","first-page":"1-11","DOI":"10.1186\/s40709-020-00115-4","article-title":"A review on Zika virus outbreak, epidemiology, transmission and infection dynamics.","volume":"27","author":"S Kazmi","year":"2020","journal-title":"J. Biol. Res (Thessalon)."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"567-574","DOI":"10.1590\/1413-81232020252.30002017","article-title":"Analysis of the concept of the Zika Virus congenital syndrome\/Analise do conceito sindrome congenita pelo Zika virus.","volume":"25","author":"G Teixeira","year":"2020","journal-title":"Cien. Saude Colet."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"910-914","DOI":"10.15585\/mmwr.mm6534e1","article-title":"Guillain-Barr\u00e9 syndrome during ongoing zika virus transmission\u2014Puerto Rico, January 1\u2013July 31, 2016.","volume":"65","author":"E Dirlikov","year":"2016","journal-title":"Morb. Mortal. Wkly Rep."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"73-83","DOI":"10.1128\/JVI.72.1.73-83.1998","article-title":"Phylogeny of the genus Flavivirus.","volume":"72","author":"G Kuno","year":"1998","journal-title":"J. Virol."},{"key":"ref81","first-page":"54-56","article-title":"Ophthalmologic Findings in a Premature Infant leading to a Zika Diagnosis during the COVID-19 Pandemic: A Case Report.","volume":"43","author":"J Rosario-Falero","year":"2024","journal-title":"P. R. Health Sci. J."},{"key":"ref82","doi-asserted-by":"publisher","first-page":"576-579","DOI":"10.1111\/tmi.13900","article-title":"Zika virus infection in European travellers returning from Thailand in 2022: A GeoSentinel case series.","volume":"28","author":"T Seers","year":"2023","journal-title":"Trop. Med. Int. Health."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1552-1563","DOI":"10.1056\/NEJMra1602113","article-title":"Zika virus.","volume":"374","author":"L Petersen","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"16212-16215","DOI":"10.1021\/jacs.6b10399","article-title":"Structural insight into NS5 of Zika virus leading to the discovery of MTase inhibitors.","volume":"138","author":"P Stephen","year":"2016","journal-title":"J. Am. Chem. Soc."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"385-390","DOI":"10.1002\/cmdc.201900533","article-title":"Design, Synthesis and Discovery of N, N\u2019-Carbazoyl-aryl-urea Inhibitors of Zika NS5 Methyltransferase and Virus Replication.","volume":"15","author":"S Spizzichino","year":"2020","journal-title":"ChemMedChem."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"e00411-e00417","DOI":"10.1128\/AAC.00411-17","article-title":"The race to find antivirals for Zika virus.","volume":"61","author":"J Saiz","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"e02202-e02216","DOI":"10.1128\/JVI.02202-16","article-title":"Zika virus methyltransferase: structure and functions for drug design perspectives.","volume":"91","author":"B Coutard","year":"2017","journal-title":"J. Virol."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"502-506","DOI":"10.1016\/j.ijantimicag.2019.07.010","article-title":"Identification and characterization of Zika virus NS5 RNA-dependent RNA polymerase inhibitors.","volume":"54","author":"Y Lin","year":"2019","journal-title":"Int. J. Antimicrob. Agents."},{"key":"ref13","article-title":"IN-SILICO STUDY OF TOXICITY MECHANISMS FOR METABOLITES OF PHYTO-COMPOUNDS FROM MUSA SP. COMPARED TO SYNTHETIC MEDICINE RANITIDINE.","author":"D Choudhury"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"699-713","DOI":"10.1080\/19390211.2018.1472715","article-title":"The prevention and therapy of osteoporosis: a review on emerging trends from hormonal therapy to synthetic drugs to plant-based bioactives.","volume":"16","author":"T Gupta","year":"2019","journal-title":"J. Diet. Suppl."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"105649","DOI":"10.1016\/j.bioorg.2022.105649","article-title":"Chalcones from Angelica keiskei (ashitaba) inhibit key Zika virus replication proteins.","volume":"120","author":"M Mottin","year":"2022","journal-title":"Bioorg. Chem."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"1739-1780","DOI":"10.1002\/med.21891","article-title":"Natural products as Zika antivirals.","volume":"42","author":"Y Fong","year":"2022","journal-title":"Med. Res. 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Sci."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"217-238","DOI":"10.1007\/978-981-13-0347-0_13","article-title":"Pharmacokinetic and molecular docking studies of plant-derived natural compounds to exploring potential anti-Alzheimer activity.","author":"A Alam","year":"2018","journal-title":"In Silico Approach for Sustainable Agriculture."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"101297","DOI":"10.1016\/j.jksus.2020.101297","article-title":"Evaluating the potency of Sulawesi propolis compounds as ACE-2 inhibitors through molecular docking for COVID-19 drug discovery preliminary study.","volume":"33","author":"A Khayrani","year":"2021","journal-title":"J. King Saud Univ. Sci."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"529-555","DOI":"10.1007\/s12010-021-03698-3","article-title":"GC\u2013MS Analysis, molecular docking and pharmacokinetic properties of phytocompounds from Solanum torvum unripe fruits and its effect on breast cancer target protein.","volume":"194","author":"R Saravanan","year":"2022","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"5182-5208","DOI":"10.1080\/07391102.2020.1785944","article-title":"In silico docking of phytocompounds to identify potent inhibitors of signaling pathways involved in prostate cancer.","volume":"39","author":"B Jonnalagadda","year":"2021","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref24","first-page":"3460-3467","article-title":"Benefits of papaya fruit and its leaves to treat malaria or dengue and various other uses for human health.","volume":"8","author":"M Deshpande","year":"2021","journal-title":"Int. Res. J. Eng. Technol."},{"key":"ref83","first-page":"11-15","article-title":"Potential role of carica papaya and their active constituents in the prevention and treatment of diseases.","volume":"8","author":"A Rahmani","year":"2016","journal-title":"Int. J. Pharm. Pharm. Sci."},{"key":"ref25","first-page":"2201-2205","article-title":"Phytochemical and antioxidant nutrient constituents of Carica papaya and Parquetina nigrescens extracts.","volume":"5","author":"N Imaga","year":"2010","journal-title":"Sci. Res. Essays."},{"key":"ref84","doi-asserted-by":"publisher","first-page":"234-248","DOI":"10.30574\/gscbps.2021.14.3.0073","article-title":"Morphology, phytochemistry and pharmacological aspects of Carica papaya, an review.","volume":"14","author":"A Wadekar","year":"2021","journal-title":"GSC Biol. Pharm. Sci."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"1-17","DOI":"10.1007\/s12010-023-04473-2","article-title":"Discovery of Potential Phytochemicals from Carica papaya Targeting BRCA-1 in Breast Cancer Treatment.","author":"A Ibrahim","year":"2023","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"202-207","DOI":"10.5530\/ijpi.2020.2.37","article-title":"Spatial Long-Term Memory Retention by Banana and Papaya Peel Extract: In silico and in vivo Evaluation.","volume":"10","author":"K Reddy","year":"2020","journal-title":"Int. J. Pharm. Investig."},{"key":"ref31","first-page":"264-278","article-title":"Molecular docking and pharmacokinetics study for selected leaf phytochemicals from Carica papaya Linn. against dengue virus protein, NS2B\/NS3 protease.","volume":"124","author":"I Ghosh","year":"2019","journal-title":"World Sci. News."},{"key":"ref32","doi-asserted-by":"publisher","author":"K Kumaree","year":"2024","DOI":"10.5281\/zenodo.12057456"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"889-895","DOI":"10.6026\/97320630009889","article-title":"Flavonoid from Carica papaya inhibits NS2B-NS3 protease and prevents Dengue 2 viral assembly.","volume":"9","author":"P Senthilvel","year":"2013","journal-title":"Bioinformation."},{"key":"ref34","article-title":"Molecular docking analysis of Carica papaya Linn constituents as antiviral agent.","volume":"24","author":"N Radhakrishnan","year":"2017","journal-title":"Int. Food Res. J."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"584-590","DOI":"10.1016\/j.jfca.2007.03.009","article-title":"Gas chromatography\u2013mass spectrometry analysis of phenolic compounds from Carica papaya L. leaf.","volume":"20","author":"A Canini","year":"2007","journal-title":"J. Food Compos. Anal."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"243-250","DOI":"10.1007\/978-1-4939-2269-7_19","article-title":"Small-molecule library screening by docking with PyRx.","author":"S Dallakyan","year":"2015","journal-title":"Chemical biology: methods and protocols."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"14830-14834","DOI":"10.18632\/oncotarget.14780","article-title":"The conformational changes of Zika virus methyltransferase upon converting SAM to SAH.","volume":"8","author":"H Zhou","year":"2017","journal-title":"Oncotarget."},{"key":"ref85","doi-asserted-by":"publisher","first-page":"409-418","DOI":"10.1080\/1062936X.2018.1454981","article-title":"Molecular docking revealed the binding of nucleotide\/side inhibitors to Zika viral polymerase solved structures.","volume":"29","author":"A Elfiky","year":"2018","journal-title":"SAR QSAR Environ. Res."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"3958-3978","DOI":"10.1021\/acs.jmedchem.7b00259","article-title":"Nitrile in the hole: discovery of a small auxiliary pocket in neuronal nitric oxide synthase leading to the development of potent and selective 2-aminoquinoline inhibitors.","volume":"60","author":"M Cinelli","year":"2017","journal-title":"J. Med. Chem."},{"key":"ref86","doi-asserted-by":"publisher","first-page":"2091-2096","DOI":"10.1007\/s00705-017-3345-x","article-title":"Structural basis of Zika virus methyltransferase inhibition by sinefungin.","volume":"162","author":"K Hercik","year":"2017","journal-title":"Arch. Virol."},{"key":"ref88","doi-asserted-by":"publisher","first-page":"40920","DOI":"10.1038\/srep40920","article-title":"The clinically approved antiviral drug sofosbuvir inhibits Zika virus replication.","volume":"7","author":"C Sacramento","year":"2017","journal-title":"Sci. Rep."},{"key":"ref89","doi-asserted-by":"publisher","first-page":"994-1004","DOI":"10.1016\/j.ejmech.2018.08.057","article-title":"Design, synthesis and in vitro anti-Zika virus evaluation of novel Sinefungin derivatives.","volume":"157","author":"Z Tao","year":"2018","journal-title":"Eur. J. Med. Chem."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"12-21","DOI":"10.1107\/S0907444909042073","article-title":"MolProbity: all-atom structure validation for macromolecular crystallography.","volume":"66","author":"V Chen","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"3891-3898","DOI":"10.1021\/acs.jcim.1c00203","article-title":"AutoDock Vina 1.2. 0: New docking methods, expanded force field, and python bindings.","volume":"61","author":"J Eberhardt","year":"2021","journal-title":"J. Chem. Inf. Model."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"455-461","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading.","volume":"31","author":"O Trott","year":"2010","journal-title":"J. Comput. Chem."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"230","DOI":"10.12688\/f1000research.130618.1","article-title":"In silico molecular docking and molecular dynamic simulation of agarwood compounds with molecular targets of Alzheimer\u2019s disease.","volume":"12","author":"P Alugoju","year":"2023","journal-title":"F1000Res."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"3-25","DOI":"10.1016\/S0169-409X(96)00423-1","article-title":"Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings.","volume":"23","author":"C Lipinski","year":"1997","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"401-408","DOI":"10.1097\/QCO.0000000000000775","article-title":"Global health security as it pertains to Zika, Ebola, and COVID-19.","volume":"34","author":"J Pannu","year":"2021","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/v15010001","article-title":"A Glimpse on the Evolution of RNA Viruses: Implications and Lessons from SARS-CoV-2.","volume":"15","author":"P \u0160imi\u010di\u0107","year":"2022","journal-title":"Viruses."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"1531-1539","DOI":"10.1016\/S0140-6736(16)00562-6","article-title":"Guillain-Barr\u00e9 Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study.","volume":"387","author":"V Cao-Lormeau","year":"2016","journal-title":"Lancet."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"1481","DOI":"10.1016\/S0140-6736(16)00644-9","article-title":"Acute myelitis due to Zika virus infection.","volume":"387","author":"S M\u00e9charles","year":"2016","journal-title":"Lancet."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"1595-1596","DOI":"10.1056\/NEJMc1602964","article-title":"Zika virus associated with meningoencephalitis.","volume":"374","author":"G Carteaux","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"394-396","DOI":"10.1056\/NEJMc1603618","article-title":"Uveitis associated with Zika virus infection.","volume":"375","author":"J Furtado","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"330-333","DOI":"10.1016\/S2221-1691(11)60055-5","article-title":"Dengue fever treatment with Carica papaya leaves extracts.","volume":"1","author":"N Ahmad","year":"2011","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref52","doi-asserted-by":"crossref","first-page":"1-8","DOI":"10.1186\/s12906-019-2678-2","article-title":"Carica papaya extract in dengue: a systematic review and meta-analysis.","volume":"19","author":"S Rajapakse","year":"2019","journal-title":"BMC Complement. Altern. Med."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"1516","DOI":"10.3390\/nu13051516","article-title":"Anti-inflammatory and immunomodulatory properties of fermented plant foods.","volume":"13","author":"R Shahbazi","year":"2021","journal-title":"Nutrients."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"2043-2056","DOI":"10.1080\/13880209.2017.1346690","article-title":"Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leaves.","volume":"55","author":"V Anjum","year":"2017","journal-title":"Pharm. Biol."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"624-630","DOI":"10.1016\/j.bbrc.2016.11.098","article-title":"Structure of the NS5 methyltransferase from Zika virus and implications in inhibitor design.","volume":"492","author":"C Zhang","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"867-874","DOI":"10.3923\/pjbs.2022.867.874","article-title":"In silico Study Phytosterol Cymbopogon citratus and Curcuma longa as Inhibitor Agent 3C-Like Protease SARS-CoV-2.","volume":"25","author":"T Watuguly","year":"2022","journal-title":"Pak. J. Biol. Sci."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"57-67","DOI":"10.1016\/j.antiviral.2015.04.010","article-title":"The dengue virus NS5 protein as a target for drug discovery.","volume":"119","author":"S Lim","year":"2015","journal-title":"Antivir. Res."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"e12029","DOI":"10.1371\/journal.pone.0012029","article-title":"Optimized hydrophobic interactions and hydrogen bonding at the target-ligand interface leads the pathways of drug-designing.","volume":"5","author":"R Patil","year":"2010","journal-title":"PLoS One."},{"key":"ref91","doi-asserted-by":"publisher","first-page":"174-186","DOI":"10.1016\/j.pbiomolbio.2014.05.006","article-title":"Polypharmacology rescored: Protein\u2013ligand interaction profiles for remote binding site similarity assessment.","volume":"116","author":"S Salentin","year":"2014","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"919-933","DOI":"10.15252\/embj.201696241","article-title":"The crystal structure of Zika virus NS 5 reveals conserved drug targets.","volume":"36","author":"W Duan","year":"2017","journal-title":"EMBO J."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"14764","DOI":"10.1038\/ncomms14764","article-title":"Crystal structure of Zika virus NS5 RNA-dependent RNA polymerase.","volume":"8","author":"A Godoy","year":"2017","journal-title":"Nat. Commun."},{"key":"ref61","doi-asserted-by":"crossref","first-page":"1-13","DOI":"10.1038\/s41598-020-70094-y","article-title":"Identification of the viral RNA promoter stem loop A (SLA)-binding site on Zika virus polymerase NS5.","volume":"10","author":"P Bujalowski","year":"2020","journal-title":"Sci. Rep."},{"key":"ref62","first-page":"208-212","article-title":"Beta-sitosterol medicinal properties: A review article.","volume":"9","author":"K Rashed","year":"2020","journal-title":"J. Sci. Innov. Technol."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"228","DOI":"10.3390\/vaccines11020228","article-title":"Phytoestrogen \u03b2-Sitosterol Exhibits Potent in vitro Antiviral Activity against Influenza A Viruses.","volume":"11","author":"S Shokry","year":"2023","journal-title":"Vaccines."},{"key":"ref64","doi-asserted-by":"publisher","first-page":"193-210","DOI":"10.2174\/187152109788680207","article-title":"Sterols and triterpenoids with antiviral activity.","volume":"8","author":"T Rezanka","year":"2009","journal-title":"Anti-Infective Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Infective Agents)."},{"key":"ref65","doi-asserted-by":"publisher","first-page":"43-49","DOI":"10.1006\/cbir.2000.0676","article-title":"Flow cytometric analysis of the Th1\u2013Th2 balance in healthy individuals and patients infected with the human immunodeficiency virus (HIV) receiving a plant sterol\/sterolin mixture.","volume":"25","author":"U Breytenbach","year":"2001","journal-title":"Cell Biol. Int."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"11474-11489","DOI":"10.3390\/molecules200611474","article-title":"Natural plant alkaloid (emetine) inhibits HIV-1 replication by interfering with reverse transcriptase activity.","volume":"20","author":"A Valad\u00e3o","year":"2015","journal-title":"Molecules."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"696","DOI":"10.3390\/biom9110696","article-title":"Natural bis-benzylisoquinoline alkaloids-tetrandrine, fangchinoline, and cepharanthine, inhibit human coronavirus OC43 infection of MRC-5 human lung cells.","volume":"9","author":"D Kim","year":"2019","journal-title":"Biomolecules."},{"key":"ref68","doi-asserted-by":"publisher","first-page":"484-488","DOI":"10.1128\/AAC.39.2.484","article-title":"Michellamine B, a novel plant alkaloid, inhibits human immunodeficiency virus-induced cell killing by at least two distinct mechanisms.","volume":"39","author":"J McMahon","year":"1995","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref69","doi-asserted-by":"publisher","first-page":"653-655","DOI":"10.1016\/S0022-3476(97)70253-1","article-title":"Reduced plasma concentrations of alpha-tocopherol and beta-carotene in obese boys.","volume":"130","author":"T Decsi","year":"1997","journal-title":"J. Pediatr."},{"key":"ref70","doi-asserted-by":"publisher","first-page":"235-241","DOI":"10.1007\/BF00685083","article-title":"In vivo modulation of several anticancer agents by \u03b2-carotene.","volume":"34","author":"B Teicher","year":"1994","journal-title":"Cancer Chemother. Pharmacol."},{"key":"ref71","doi-asserted-by":"publisher","first-page":"531","DOI":"10.3390\/md19100531","article-title":"Anti-inflammatory and anticancer effects of microalgal carotenoids.","volume":"19","author":"J \u00c1vila-Rom\u00e1n","year":"2021","journal-title":"Mar. Drugs."},{"key":"ref72","doi-asserted-by":"publisher","first-page":"731-741","DOI":"10.1007\/s10811-011-9692-1","article-title":"Antiviral compounds obtained from microalgae commonly used as carotenoid sources.","volume":"24","author":"S Santoyo","year":"2012","journal-title":"J. Appl. Phycol."},{"key":"ref73","doi-asserted-by":"publisher","first-page":"3979-3988","DOI":"10.1007\/s00253-015-7242-5","article-title":"Antiviral activity and specific modes of action of bacterial prodigiosin against Bombyx mori nucleopolyhedrovirus in vitro.","volume":"100","author":"W Zhou","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref74","doi-asserted-by":"publisher","first-page":"2169-2175","DOI":"10.1016\/j.foodchem.2012.04.024","article-title":"Regulation of virus-induced inflammatory response by \u03b2-carotene in RAW264. 7 cells.","volume":"134","author":"H Lin","year":"2012","journal-title":"Food Chem."},{"key":"ref75","doi-asserted-by":"publisher","first-page":"241-261","DOI":"10.3746\/pnf.2021.26.3.241","article-title":"Carotenoids: Therapeutic strategy in the battle against viral emerging diseases, COVID-19: An overview.","volume":"26","author":"A Khalil","year":"2021","journal-title":"Prev. Nutr. Food Sci."},{"key":"ref76","first-page":"797-805","article-title":"Pharmacokinetic study with computational tools in the medicinal chemistry course.","volume":"47","author":"M Brito","year":"2011","journal-title":"Brazilian. J. Pharm. Sci."},{"key":"ref77","first-page":"403-449","article-title":"Role of pharmacokinetics and metabolism in drug discovery and development.","volume":"49","author":"J Lin","year":"1997","journal-title":"Pharmacol. Rev."},{"key":"ref78","doi-asserted-by":"publisher","first-page":"677-679","DOI":"10.3201\/eid1804.111522","article-title":"Dengue in patients with central nervous system manifestations, Brazil.","volume":"18","author":"F Ara\u00fajo","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref79","doi-asserted-by":"publisher","first-page":"194-204","DOI":"10.1124\/mi.3.4.194","article-title":"Cytochromes P450, drugs, and diseases.","volume":"3","author":"F Guengerich","year":"2003","journal-title":"Mol. Interv."},{"key":"ref80","doi-asserted-by":"crossref","first-page":"1-10","DOI":"10.1186\/1743-7075-5-27","article-title":"Role of cytochrome P450 in drug interactions.","volume":"5","author":"Z Bibi","year":"2008","journal-title":"Nutr. 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