{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:08:14Z","timestamp":1772168894862,"version":"3.50.1"},"reference-count":63,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:00:00Z","timestamp":1690329600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009104","name":"Department of Health Research, India","doi-asserted-by":"publisher","award":["1101-2021"],"award-info":[{"award-number":["1101-2021"]}],"id":[{"id":"10.13039\/501100009104","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background:<\/ns4:bold>\n                    Tongue tumors show intra and inter-tumoral heterogenicity with high incidence, relapse and mortality rates necessitating further research.\u00a0 Recurrence\/metastasis that occurs\u00a0 after surgical resection of primary cancer is often the reason for poor survival in these patients.\u00a0 Lymph nodes are the most common site of metastasis in tongue tumors. Therefore, premetastatic molecular changes can be best evaluated in lymph nodes which may epitomize the earliest events in the metastasis cascades. The presence of circulating tumor cells(CTCs) in the absence of nodal disease (N0) may represent tumor aggressiveness, suggesting an immune escape which may have high metastatic potential. This trial\u00a0 was developed\u00a0 to investigate the earliest pre-metastatic changes which may regulate tumor dormancy and predict metastasis. A better understanding of organotropism or pre-metastatic changes can help in theragnostic, thereby\u00a0 preventing the outbreak of overt metastasis.\u00a0\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    A single-institutional prospective observational cohort study. This trial will be conducted at a tertiary care Centre (Amrita Institute of Medical Sciences Kochi).\u00a0 Eligible patients will be enrolled after obtaining informed consent. The dissected lymph nodes will\u00a0 be subjected to histopathological and immunohistochemical analyses for premetastatic niche (PMN) formation. In addition, circulating tumor cells will be evaluated before treatment and 6 months after treatment. The patients will be followed\u00a0 up for a period of two years to correlate the findings with the recurrence-free survival.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Expected results:<\/ns4:bold>\n                    \u00a0The pre-metastatic changes, if detected will\u00a0 be\u00a0 a predictive biomarker. It may help to define future drug targets for metastasis chemoprevention\u00a0 \u00a0. CTCs may\u00a0 define the tumor aggressiveness ,there by \u00a0prognostication \u00a0and helps in better disease management.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Ethics and\u00a0dissemination:<\/ns4:bold>\n                    The study has received the following approval:\n                  <\/ns4:p>\n                  <ns4:p>Ethics Committee of Amrita School of Medicine (ECASM-AIMS-2022-048).Trial Registered Prospectively( CTRI\/2022\/03\/041256 ) on 22\/03\/2022 under Clinical Trial Registry of India<\/ns4:p>","DOI":"10.12688\/f1000research.138951.1","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T13:28:17Z","timestamp":1690378097000},"page":"889","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of\u00a0 premetastatic changes in lymph nodes(pN0) of oral tongue tumour: A prospective observational Study"],"prefix":"10.12688","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0917-8772","authenticated-orcid":false,"given":"Rajalakshmi","family":"Geetha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subramania","family":"Iyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavithran","family":"Keechilat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gopalakrishna Iyer","family":"N","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishna Kumar","family":"Thankappan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Smitha","family":"N V","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2023,7,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"209-249","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries.","volume":"71","author":"H Sung","year":"2021","journal-title":"CA Cancer J. 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