{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:14:48Z","timestamp":1790072088095,"version":"4.0.1"},"reference-count":28,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T00:00:00Z","timestamp":1535068800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background<\/ns4:bold>\n                    <ns4:bold>:<\/ns4:bold>\n                    \u00a0Hepatocellular carcinoma (HCC) is vital medical issue in Egypt. It accounts for 70.48% of all liver tumors among Egyptians. The aim of this study was to determine the diagnostic role of plasma levels of mRNA of lamin B1 by RT-qPCR as an early marker of HCC.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    This study was conducted at the Clinical Pathology Department in collaboration with the Department of Tropical Medicine and Infectious Diseases at Ain Shams University Hospitals. It included 30 patients with primary HCC and viral cirrhosis (all were hepatitis C virus-positive) (Group I), in addition to 10 patients with chronic liver diseases (Group II) and 10 healthy age- and sex-matched subjects (Group III). Group I was further classified according to the Barcelona-Clinic Liver Cancer Staging System. Serum \u03b1-fetoprotein (AFP) chemiluminescent-immunoassays and RT-qPCR analysis of plasma lamin B1 mRNA levels were performed for all participants.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results:<\/ns4:bold>\n                    AFP and lamin B1 significantly elevated in patients with HCC compared to those in the other studied groups. AFP and lamin B1 status could discriminate group I from group II and III. A significant increase was found among the three Barcelona stages with regards to AFP and lamin B1 levels. A significant decrease was found between group II and stage 0, A and B with regards to AFP and lamin B1. Lamin B1 and AFP could both differentiate HCC patients with one tumor nodule (T1) from those with two or more tumor nodules (T2&amp;Tm), as well as between those with tumor sizes &gt;3 cm and \u22643 cm.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusion:<\/ns4:bold>\n                    Measurement of lamin B1 mRNA is recommended in patients with chronic liver disease with normal serum AFP, especially in known cirrhotic patients that deteriorate rapidly without any apparent etiology.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.14795.1","type":"journal-article","created":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T11:45:46Z","timestamp":1535111146000},"page":"1339","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Using Lamin B1 mRNA for the early diagnosis of hepatocellular carcinoma: a cross-sectional diagnostic accuracy 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Gastroenterol."},{"key":"ref-4","doi-asserted-by":"publisher","first-page":"1858-1870","DOI":"10.9734\/BJMMR\/2013\/4187","article-title":"Tissue biomarkers in the early detection of hepatocellular carcinoma among egyptian patients with chronic hepatitis C: A possible genetic profile.","volume":"3","author":"H El-Garem","year":"2013","journal-title":"Br J Med Med Res."},{"key":"ref-5","doi-asserted-by":"publisher","first-page":"645-646","DOI":"10.4161\/cc.9.4.10844","article-title":"New messages in the nuclear envelope.","volume":"9","author":"W Dauer","year":"2010","journal-title":"Cell Cycle."},{"key":"ref-6","doi-asserted-by":"publisher","first-page":"584-98","DOI":"10.1016\/j.cell.2013.01.009","article-title":"LBR and lamin A\/C sequentially tether peripheral heterochromatin and inversely regulate differentiation.","volume":"152","author":"I Solovei","year":"2013","journal-title":"Cell."},{"key":"ref-7","doi-asserted-by":"publisher","first-page":"967-1008","DOI":"10.1152\/physrev.00047.2005","article-title":"Nuclear lamins: laminopathies and their role in premature ageing.","volume":"86","author":"J Broers","year":"2006","journal-title":"Physiol Rev."},{"key":"ref-8","doi-asserted-by":"publisher","first-page":"3537-3544","DOI":"10.1093\/hmg\/ddr266","article-title":"An absence of both lamin B1 and lamin B2 in keratinocytes has no effect on cell proliferation or the development of skin and hair.","volume":"20","author":"S Yang","year":"2011","journal-title":"Hum Mol Genet."},{"key":"ref-9","doi-asserted-by":"publisher","first-page":"1706-10","DOI":"10.1126\/science.1211222","article-title":"Mouse B-type lamins are required for proper organogenesis but not by embryonic stem cells.","volume":"334","author":"Y Kim","year":"2011","journal-title":"Science."},{"key":"ref-10","doi-asserted-by":"publisher","first-page":"609-13","DOI":"10.3892\/or.15.3.609","article-title":"Differential expression of nuclear lamins in normal and cancerous prostate tissues.","volume":"15","author":"R Coradeghini","year":"2006","journal-title":"Oncol Rep."},{"key":"ref-11","doi-asserted-by":"publisher","first-page":"297-300","DOI":"10.1042\/BST0380297","article-title":"Lamins as cancer biomarkers.","volume":"38","author":"C Foster","year":"2010","journal-title":"Biochem Soc Trans."},{"key":"ref-12","doi-asserted-by":"publisher","first-page":"1020-1022","DOI":"10.1002\/hep.24199","article-title":"Management of hepatocellular carcinoma: an update.","volume":"53","author":"J Bruix","year":"2011","journal-title":"Hepatology."},{"key":"ref-13","doi-asserted-by":"publisher","first-page":"646-649","DOI":"10.1002\/bjs.1800600817","article-title":"Transection of the oesophagus for bleeding oesophageal varices.","volume":"60","author":"R Pugh","year":"1973","journal-title":"Br J Surg."},{"key":"ref-14","doi-asserted-by":"publisher","first-page":"995-1001","DOI":"10.1016\/j.jhep.2014.01.006","article-title":"Clinical and economical impact of 2010 AASLD guidelines for the diagnosis of hepatocellular carcinoma.","volume":"60","author":"M Manini","year":"2014","journal-title":"J Hepatol."},{"key":"ref-15","doi-asserted-by":"publisher","first-page":"1383-1393","DOI":"10.1100\/tsw.2011.124","article-title":"Linear methods for analysis and quality control of relative expression ratios from quantitative real-time polymerase chain reaction experiments.","volume":"11","author":"R Page","year":"2011","journal-title":"Scientific World Journal."},{"key":"ref-16","first-page":"25","article-title":"Shanghai, China Hepatobiliary.","volume":"5","author":"J Wei","year":"2006","journal-title":"Pancr Dis Int."},{"key":"ref-17","doi-asserted-by":"publisher","first-page":"295-310","DOI":"10.1007\/978-1-61779-959-4_19","article-title":"Discovery of lamin B1 and vimentin as circulating biomarkers for early hepatocellular carcinoma","volume":"909","author":"K Wong","year":"2012"},{"key":"ref-18","doi-asserted-by":"publisher","first-page":"70-78","DOI":"10.1021\/pr9002118","article-title":"Circulating Lamin B1 (LMNB1) biomarker detects early stages of liver cancer in patients.","volume":"9","author":"S Sun","year":"2010","journal-title":"J Proteome Res."},{"key":"ref-19","doi-asserted-by":"publisher","first-page":"82-88","DOI":"10.1016\/j.canep.2011.04.001","article-title":"Screening of autoantibodies as potential biomarkers for hepatocellular carcinoma by using T7 phase display system.","volume":"36","author":"H Liu","year":"2012","journal-title":"Cancer Epidemiol."},{"key":"ref-20","doi-asserted-by":"publisher","first-page":"44-50","DOI":"10.1002\/ijc.20279","article-title":"High alpha-fetoprotein level correlates with high stage, early recurrence and poor prognosis of hepatocellular carcinoma: significance of hepatitis virus infection, age, p53 and beta-catenin mutations.","volume":"112","author":"S Peng","year":"2004","journal-title":"Int J Cancer."},{"key":"ref-21","doi-asserted-by":"publisher","first-page":"81389-81401","DOI":"10.18632\/oncotarget.13232","article-title":"Incorporation of alpha-fetoprotein(AFP) into subclassification of BCLC C stage hepatocellular carcinoma according to a 5-year survival analysis based on the SEER database.","volume":"7","author":"N Zhang","year":"2016","journal-title":"Oncotarget."},{"key":"ref-22","doi-asserted-by":"publisher","first-page":"1031-1037","DOI":"10.1006\/bbrc.2002.6547","article-title":"Proteome analysis of hepatocellular carcinoma.","volume":"291","author":"S Lim","year":"2002","journal-title":"Biochem Biophys Res Commun."},{"key":"ref-23","doi-asserted-by":"publisher","first-page":"524-532","DOI":"10.1111\/j.1572-0241.2006.00443.x","article-title":"Diagnostic and prognostic role of alpha-fetoprotein in hepatocellular carcinoma: both or neither?","volume":"101","author":"F Farinati","year":"2006","journal-title":"Am J Gastroenterol."},{"key":"ref-24","first-page":"1705-1709","article-title":"Serum alpha-fetoprotein level per tumor volume reflects prognosis in patients with hepatocellular carcinoma after curative hepatectomy.","volume":"55","author":"T Furihata","year":"2008","journal-title":"Hepatogastroenterology."},{"key":"ref-25","doi-asserted-by":"publisher","first-page":"957-66","DOI":"10.1096\/fj.08-121244","article-title":"Involvement of nuclear PLCbeta1 in lamin B1 phosphorylation and G2\/M cell cycle progression.","volume":"23","author":"R Fiume","year":"2009","journal-title":"FASEB J."},{"key":"ref-26","first-page":"404-411","article-title":"[Optimal cut-off value of PIVKA-II for diagnosis of hepatocellular carcinoma--using ROC curve].","volume":"12","author":"M Kim","year":"2006","journal-title":"Korean J Hepatol."},{"key":"ref-27","doi-asserted-by":"publisher","first-page":"1155-1165","DOI":"10.1634\/theoncologist.2008-0081","article-title":"Osteopontin combined with CD44, a novel prognostic biomarker for patients with hepatocellular carcinoma undergoing curative resection.","volume":"13","author":"G Yang","year":"2008","journal-title":"Oncologist."},{"key":"ref-28","doi-asserted-by":"crossref","DOI":"10.12688\/f1000research.14795.1","article-title":"Dataset 1 in: Using Lamin B1 mRNA for the early diagnosis of hepatocellular carcinoma: a cross-sectional diagnostic accuracy study.","author":"A 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status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.16100.r37615, Dina Fekry, Department of Clinical Pathology, National Research Centre\u00a0(NRC), Cairo, Egypt, 06 Sep 2018, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/7-1339\/v1#referee-response-37615","order":0,"name":"referee-response-37615","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.16100.r39775, Sherief Abd-Elsalam, Department of Tropical Medicine\u00a0and\u00a0Infectious Diseases, Faculty of Medicine, Tanta University, Tanta, Egypt, 30 Oct 2018, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/7-1339\/v1#referee-response-39775","order":1,"name":"referee-response-39775","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"The author(s) declared that no grants were involved in supporting this work","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Data associated with the article are available under the terms of the Creative Commons Zero \"No rights reserved\" data waiver (CC0 1.0 Public domain dedication).","order":0,"name":"copyright-info","label":"Copyright"}]}}