{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T12:32:06Z","timestamp":1777984326770,"version":"3.51.4"},"update-to":[{"DOI":"10.12688\/f1000research.149829.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000}}],"reference-count":16,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["P30AG024409"],"award-info":[{"award-number":["P30AG024409"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>\n                    Background Vitamin D may play an important role in later-life physical and cognitive health. Vitamin D status is standardly assessed in serum and plasma; however, collection, transport, and storage costs make large epidemiologic studies challenging. We assessed the agreement of 25-hydroxyvitamin D\n                    <ns3:sub>3<\/ns3:sub>\n                    (25(OH)D\n                    <ns3:sub>3<\/ns3:sub>\n                    ) quantification from dried blood spots (DBS) as compared to standard plasma assessment among older Indian adults. Methods A total of 58 adults over 45 years of age who resided in Pune, India were enrolled in the study from July 2020 to June 2021. Liquid chromatography-tandem mass spectrometry (LC-MS\/MS) was used to assess 25(OH)D\n                    <ns3:sub>3<\/ns3:sub>\n                    concentrations in paired plasma and DBS samples. Results Plasma and DBS 25(OH)D\n                    <ns3:sub>3<\/ns3:sub>\n                    concentrations were highly correlated (Pearson\u2019s correlation = 0.976). The median 25(OH)D\n                    <ns3:sub>3<\/ns3:sub>\n                    concentration of the study population assessed by plasma was 14.6 ng\/mL (Q1=12.0, Q3= 18.1) while the median concentration assessed in DBS was 12.8 ng\/mL (Q1=11.0, Q3= 16.6). 25(OH)D\n                    <ns3:sub>3<\/ns3:sub>\n                    concentrations measured from DBS were on average 6% (95% CI: 2-13%) lower than concentrations assessed by plasma across the observed 25(OH)D\n                    <ns3:sub>3<\/ns3:sub>\n                    distribution. Conclusions We found good agreement between 25(OH)D\n                    <ns3:sub>3<\/ns3:sub>\n                    quantification between DBS and plasma and our findings indicate that DBS can be used in epidemiologic studies of vitamin D among Indian adults.\n                  <\/ns3:p>","DOI":"10.12688\/f1000research.149829.2","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T08:10:07Z","timestamp":1751271007000},"page":"512","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantification of 25-hydroxyvitamin D3 in dried blood spots as compared to plasma among Indian adults"],"prefix":"10.12688","volume":"13","author":[{"given":"Ashwini V","family":"Shete","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1702-3631","authenticated-orcid":false,"given":"Jyoti","family":"Sawant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajani","family":"Bagul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ujjwala","family":"Ghule","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarang S.","family":"Saluke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3203-3638","authenticated-orcid":false,"given":"Christopher R.","family":"Sudfeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2025,6,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"297-300","DOI":"10.1016\/j.jsbmb.2010.02.021","article-title":"Worldwide status of vitamin D nutrition.","volume":"121","author":"P Lips","year":"2010","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"S153-S164","DOI":"10.1111\/j.1753-4887.2008.00100.x","article-title":"Vitamin D deficiency: a global perspective.","volume":"66","author":"A Prentice","year":"2008","journal-title":"Nutr. Rev."},{"key":"ref3","first-page":"207-216","article-title":"Assessment and management of nutrition in older people and its importance to health.","volume":"5","author":"T Ahmed","year":"2010","journal-title":"Clin. Interv. Aging."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"23-45","DOI":"10.1017\/S0007114513001840","article-title":"A systematic review of vitamin D status in populations worldwide.","volume":"111","author":"J Hilger","year":"2014","journal-title":"Br. J. Nutr."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"266-281","DOI":"10.1056\/NEJMra070553","article-title":"Vitamin D deficiency.","volume":"357","author":"M Holick","year":"2007","journal-title":"N. Engl. J. Med."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"73-78","DOI":"10.1016\/j.annepidem.2007.12.001","article-title":"Vitamin D status: measurement, interpretation, and clinical application.","volume":"19","author":"M Holick","year":"2009","journal-title":"Ann. Epidemiol."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"3319-3324","DOI":"10.1210\/jc.2014-1269","article-title":"Measurements of 25-hydroxyvitamin D concentrations in archived dried blood spots are reliable and accurately reflect those in plasma.","volume":"99","author":"A Heath","year":"2014","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"639-646","DOI":"10.1373\/clinchem.2015.251538","article-title":"Minimizing Matrix Effects for the Accurate Quantification of 25-Hydroxyvitamin D Metabolites in Dried Blood Spots by LC-MS\/MS.","volume":"62","author":"D Kvaskoff","year":"2016","journal-title":"Clin. Chem."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"19873","DOI":"10.1038\/s41598-020-76955-w","article-title":"A LC-MS method for 25-hydroxy-vitamin D3 measurements from dried blood spots for an epidemiological survey in India.","volume":"10","author":"R Lote-Oke","year":"2020","journal-title":"Sci. Rep."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"159-184","DOI":"10.1007\/s11154-013-9241-0","article-title":"Vitamin D and metabolites measurement by tandem mass spectrometry.","volume":"14","author":"J Ouweland","year":"2013","journal-title":"Rev. Endocr. Metab. Disord."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"105472","DOI":"10.1016\/j.jsbmb.2019.105472","article-title":"Agreement between measurement of 25-hydroxyvitamin D3 in dried blood spot samples and serum in a Chinese population in the Netherlands.","volume":"195","author":"P Man","year":"2019","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"e16602","DOI":"10.1371\/journal.pone.0016602","article-title":"Agreement of blood spot card measurements of vitamin D levels with serum, whole blood specimen types and a dietary recall instrument.","volume":"6","author":"E Larkin","year":"2011","journal-title":"PLoS One."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"156-162","DOI":"10.1177\/193229680900300118","article-title":"A liquid chromatography\/tandem mass spectrometry method for determination of 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3 in dried blood spots: a potential adjunct to diabetes and cardiometabolic risk screening.","volume":"3","author":"M Newman","year":"2009","journal-title":"J. Diabetes Sci. Technol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"2291-2300","DOI":"10.1007\/s00198-016-3537-3","article-title":"Associations of total and free 25OHD and 1,25(OH)2D with serum markers of inflammation in older men.","volume":"27","author":"P Srikanth","year":"2016","journal-title":"Osteoporos. Int."},{"key":"ref15","article-title":"Guidance for Industry, Bioanalytical Method Validation, U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER), Center for Veterinary Medicine (CMV).","year":"May 2001"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.6084\/m9.figshare.25665483.v3","author":"C Sudfeld","year":"2024","journal-title":"figshare."}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/13-512\/v2\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/13-512\/v2\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/13-512\/v2\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T04:54:10Z","timestamp":1770785650000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/13-512\/v2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,6]]},"references-count":16,"URL":"https:\/\/doi.org\/10.12688\/f1000research.149829.2","relation":{"has-version":[{"id-type":"doi","id":"10.12688\/f1000research.149829.1","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.182852.r390095","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.182852.r435234","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.182852.r390095","asserted-by":"object"},{"id-type":"doi","id":"10.5256\/f1000research.182852.r435234","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,6]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/13-512\/v2#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.164335.r300657, Christopher T Sempos, National Institutes of Health (NIH), Bethesda, MD, 13 Aug 2024, version 1, 2 approved with reservations","URL":"https:\/\/f1000research.com\/articles\/13-512\/v1#referee-response-300657","order":0,"name":"referee-response-300657","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.182852.r390095, Katarzyna Dzik, University of Gdansk, Gda\u0144sk, 30 Jun 2025, version 2, indexed","URL":"https:\/\/f1000research.com\/articles\/13-512\/v2#referee-response-390095","order":1,"name":"referee-response-390095","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.182852.r435234, Y S Phaneendra Mallimoggala, Manipal Academy of Higher Education, Manipal, Karnataka, 11 Feb 2026, version 2, indexed","URL":"https:\/\/f1000research.com\/articles\/13-512\/v2#referee-response-435234","order":2,"name":"referee-response-435234","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"The research was funded by  National Institute on Aging  Grant Number (P30AG024409). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":3,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}