{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T15:06:14Z","timestamp":1789830374826,"version":"4.0.1"},"update-to":[{"DOI":"10.12688\/f1000research.150769.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T00:00:00Z","timestamp":1725235200000}}],"reference-count":30,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T00:00:00Z","timestamp":1725235200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"No external Fund"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>\n                    Background In recent years, there has been a notable increase in the level of attention devoted to exploring capabilities of nanoparticles, specifically gold nanoparticles AuNPs, within context of modern times. AuNPs possess distinct biophysical properties, as a novel avenue as an antibacterial agent targeting Streptococcus Mutans and Candida Albicans. The aim of this study to create a nano-platform that has the potential to be environmentally sustainable, in addition to exhibiting exceptional antimicrobial properties against Streptococcus Mutans as well as Candida Albicans. Methods this study involved utilization of\n                    <ns3:italic>Pelargonium Graveolens<\/ns3:italic>\n                    leaves extract as a cost effective and environmentally sustainable approach for the green synthesis of AuNPs. Subsequently, physicochemical characteristics were assessed employing a variety of analytical methods, including as transmission electron microscopy, X-ray diffraction, Field Emission Scanning Electron Microscope, Zeta potential, Ultraviolet visible absorption spectroscopy, and Energy dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy. The antimicrobial efficacy against Streptococcus Mutans and Candida Albicans was evaluated. Nanoparticles of various shapes, including hexagonal, spherical, semi-spherical, and triangular, were synthesized. These nanoparticles exhibited a mean particle size of 294nm and demonstrated low degree of aggregation. These nanoparticles exhibited long-term stability and were capable of facilely combining with diverse bioactive compounds. Results The study demonstrated that AuNPs which is synthesized by green methods display potent antimicrobial properties. Conclusion Utilization of Pelargonium Graveolens AuNPs may exhibit a promising potential as an antibacterial agent against Streptococcus Mutans and Candida Albicans. Nanoparticles (NPs) have the potential to serve as a novel approach for addressing pathogen infections as well as for biomedical, dental and pharmaceutical purposes in the future.\n                  <\/ns3:p>","DOI":"10.12688\/f1000research.150769.2","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T10:40:06Z","timestamp":1725273606000},"page":"572","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Green synthesis of gold nanoparticles using Pelargonium Graveolens leaf extract: characterization and anti-microbial properties (An in-vitro study)"],"prefix":"10.12688","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-8038-3475","authenticated-orcid":false,"given":"Ahmed Yousif Mahdi","family":"Asker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aseel Haidar M.J.","family":"Al Haidar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2024,9,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"102064","DOI":"10.1016\/j.mtcomm.2021.102064","article-title":"Renal clearable nanoparticles: an expanding horizon for improving biomedical imaging and cancer therapy.","volume":"26","author":"M Sareh","year":"2021","journal-title":"Mater. 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I am very grateful and I agree with all your comments and revisions.  Warm&nbsp;regards,  Ahmed Yousif Mahdi Asker","URL":"https:\/\/f1000research.com\/articles\/13-572\/v2#referee-comment-11859","order":1,"name":"referee-comment-11859","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Ahmed Asker<\/b>; \n<i>Posted: 23 Jun 2024<\/i>; Dear Dr.&nbsp;Raya Abid,  \n<b>Below are the corresponding author comments with respect to your review report:<\/b>  1. Ok . I\u2019ll change the abbreviation ( AuNPs ) that appear in the abstract, by (gold nanoparticles).&nbsp;  2. Ok. I\u2019ll return to my article and will starting by capital letters.&nbsp;  3. The Reference for the isolation and preparation of microorganisms is&nbsp;( Baron E, Peteson L, Fingold S. Methods for Testing Antimicrobial Effectiveness. In: Bailey and Scotts diagnostic Microbiology. 9th ed. C.V. Mosby Co., St. Louis, USA; 1994.)&nbsp;.  4. In method the abbreviations are ,&nbsp; MIC ( minimum inhibitory concentration), MBC (minimum bactericidal concentration) and MFC ( minimum fungicidal concentration), MH-A ( Mueller-Hinton agar ) .  5. Ok dr. I\u2019ll check all the grammar and not use the pronoun 'we'.&nbsp;  6. Ok dr. I\u2019ll&nbsp;&nbsp;replace the&nbsp;FTIR with Fourier Transform Infrared.&nbsp;  7. Thanks Doctor. I\u2019ll summarize the conclusion to be should be more clear and precise as following:-&nbsp;  Conclusion&nbsp;  (The current investigation employed extracts of Pelargonium Graveolens for creating diverse shapes and dimensions of Au NPs. The successful synthesis of stable AuNPs has been supported by a range of characterization methods, including UV-Vis, Zeta potential, FESEM, EDX, XRD, TEM, and FTIR. The green synthetic gold nanoparticles form Pelargonium Graveolens leaves extract show low aggregation degree were obtained, exhibiting long-term stability. These nanoparticles can readily associate with diverse bioactive compounds. In addition, it is possible for Au NPs to combine with diverse bioactive compounds that adhere to the Pelargonium Graveolens extract, such as protein-based molecules, alkynes and phenolic compounds. This amalgamation could potentially enhance the biological activity of Au NPs that demonstrated effective antibacterial properties against Streptococcus Mutans and Candida Albicans in comparison with Chlorhexidine, while also maintaining a good level of safety. According to these results it can be considered as promising oral health care product in the future and it can be used as mouthwash as alternative of Chlorhexidine mouthwash that still had adverse effects over time.)","URL":"https:\/\/f1000research.com\/articles\/13-572\/v2#referee-comment-11862","order":2,"name":"referee-comment-11862","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.165366.r315545, Saraswathi Jaggali, Osmania University, Hyderabad, Telangana, 27 Aug 2024, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/13-572\/v1#referee-response-315545","order":3,"name":"referee-response-315545","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Ahmed Asker<\/b>; \n<i>Posted: 27 Aug 2024<\/i>; Thanks Dr.&nbsp;Saraswathi Jaggali for reviewing my manuscript.  I am very grateful and I agree with all your comments and revisions.  Warm&nbsp;regards,  Ahmed Yousif Mahdi Asker","URL":"https:\/\/f1000research.com\/articles\/13-572\/v2#referee-comment-12309","order":4,"name":"referee-comment-12309","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"The author(s) declared that no grants were involved in supporting this work","order":5,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}