{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:37:12Z","timestamp":1788953832652,"version":"build-2803163510"},"update-to":[{"DOI":"10.12688\/f1000research.160250.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000}},{"DOI":"10.12688\/f1000research.160250.2","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000}}],"reference-count":59,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005068","name":"Jimma University","doi-asserted-by":"publisher","award":["RPG\/183-2020"],"award-info":[{"award-number":["RPG\/183-2020"]}],"id":[{"id":"10.13039\/501100005068","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>\n                    Background Yeasts are unicellular fungi that inhabit a variety of environments including plant surfaces, water, soil, and animal hosts. However, limited research has been conducted on soil and plant associated yeasts in Africa, with most studies originating from developed regions. Methods This study explored the diversity, distribution, and phenotypic characterization of cultivable wild yeast in samples from rhizosphere soil, leaves, litter, and tree bark collected from South West Ethiopia. Yeast isolates were characterized using morphological, physiological and biochemical methods, Stress-tolerant yeast species were identified using Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF). Results Based on morphological, physiological, and biochemical analyses, a total of 15 yeast genera were identified from 23 plant species. Predominant yeast species included\n                    <ns3:italic>Candida<\/ns3:italic>\n                    spp.,\n                    <ns3:italic>Saccharomyces<\/ns3:italic>\n                    spp.,\n                    <ns3:italic>Meyerozyma<\/ns3:italic>\n                    spp.,\n                    <ns3:italic>Pichia<\/ns3:italic>\n                    spp.,\n                    <ns3:italic>Geotrichum<\/ns3:italic>\n                    spp., and\n                    <ns3:italic>Hanseniaspora<\/ns3:italic>\n                    spp. Plant species with the highest yeast diversity were\n                    <ns3:italic>Ficus vasta<\/ns3:italic>\n                    ,\n                    <ns3:italic>Ficus exasperata<\/ns3:italic>\n                    ,\n                    <ns3:italic>Ficus sycomorus<\/ns3:italic>\n                    ,\n                    <ns3:italic>Cordia africana<\/ns3:italic>\n                    , and\n                    <ns3:italic>Ritchiea albersii.<\/ns3:italic>\n                    Bark samples yielded more yeast isolates than rhizosphere soil, litter, and leaves. Stress-tolerant species such as\n                    <ns3:italic>Saccharomyces cerevisiae<\/ns3:italic>\n                    ,\n                    <ns3:italic>Candida pelliculosa<\/ns3:italic>\n                    ,\n                    <ns3:italic>Meyerozyma guilliermondii<\/ns3:italic>\n                    ,\n                    <ns3:italic>Pichia kluyveri<\/ns3:italic>\n                    , and\n                    <ns3:italic>Trichosporon asahii<\/ns3:italic>\n                    were identified using MALDI-TOF. Correlation analysis revealed no significant relationship between yeast populations in bark and leaf samples or between rhizosphere soil and leaves, though a weak positive correlation was found between rhizosphere soil and bark or litter. Seasonal analysis showed a strong positive correlation between yeast abundance in spring and summer, but no association between autumn and spring. Conclusion Ethiopian forests are home for various yeast species including the stress-tolerant wild yeasts. This study highlights the significant yeast diversity in Ethiopian forests, with potential applications in improving industrial fermentation processes that operate under stressful conditions.\n                  <\/ns3:p>","DOI":"10.12688\/f1000research.160250.3","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T12:39:38Z","timestamp":1744115978000},"page":"105","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Diversity, Distribution, and Phenotypic Characterization of Cultivable Wild Yeasts Isolated from Natural Forest"],"prefix":"10.12688","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0685-0669","authenticated-orcid":false,"given":"Teshome","family":"Tadesse","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Degife","family":"Dese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anbessa","family":"Dabassa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6286-5689","authenticated-orcid":false,"given":"Ketema","family":"Bacha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2025,4,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"587-601","DOI":"10.1007\/s00284-014-0755-9","article-title":"Fonseca, Yeast Biogeography and the Effects of Species Recognition Approaches: The Case Study of Widespread Basidiomycetous Species from Birch Forests in Russia.","volume":"70","author":"J Yurkov","year":"2015","journal-title":"Curr. Microbiol."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1007\/s11274-018-2518-4","article-title":"Improved bioethanol production using CRISPR\/Cas9 to disrupt the ADH2 gene in Saccharomyces cerevisiae.","volume":"34","author":"T Xue","year":"2018","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiw223","article-title":"Drivers of yeast community composition in the litter and soil of a temperate forest.","volume":"93","author":"T Ma\u0161\u00ednov\u00e1","year":"2017","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"9-19","DOI":"10.1111\/j.1567-1364.2011.00756.x","article-title":"Ecological structuring of yeasts associated with trees around.","volume":"12","author":"H Maganti","year":"2011"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.3390\/F10121096","article-title":"Estimation of fungal diversity and identification of major abiotic drivers influencing fungal richness and communities in northern temperate and boreal quebec forests.","volume":"10","author":"L Genevieve","year":"2019","journal-title":"Forests."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"1-13","DOI":"10.3390\/fermentation4040090","article-title":"Fermented foods and beverages in human diet and their influence on gut microbiota and health.","volume":"4","author":"N Carvalho","year":"2018","journal-title":"Fermentation."},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation4020036","article-title":"Citric acid production by Yarrowia lipolytica Yeast on different renewable raw materials.","volume":"4","author":"I Morgunov","year":"2018","journal-title":"Fermentation."},{"key":"ref8","first-page":"460-464","article-title":"Selection of Pichia kudriavzevii Strain for the Production of Single-Cell Protein from Cassava Processing Waste Biorefinery of Agricultural by-products View project Herbal Product Development View project.","volume":"9","author":"P Rachamontree","year":"2015","journal-title":"Int. J. Biol. Food, Vet. Agric. Eng."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"307-329","DOI":"10.1002\/elsc.201400199","article-title":"Biotechnological production of ethanol: Biochemistry, processes and technologies.","volume":"16","author":"D Sarris","year":"2016","journal-title":"Eng. Life Sci."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1-14","DOI":"10.1093\/femsyr\/fow076","article-title":"Physiology of Saccharomyces cerevisiae strains isolated from Brazilian biomes: New insights into biodiversity and industrial applications.","volume":"16","author":"F Beato","year":"2016","journal-title":"FEMS Yeast Res."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"78-85","DOI":"10.11648\/j.aas.20160103.15","article-title":"Isolation and Characterization of Yeast Inhabiting Alcohol Processing Environment in Bayelsa State, Nigeria To cite this article.","volume":"1","author":"G Rose","year":"2016"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"1-16","DOI":"10.3390\/fermentation8100515","article-title":"Lychee-Derived, Thermotolerant Yeasts for Second-Generation Bioethanol Production.","volume":"8","author":"P Van Nguyen","year":"2022","journal-title":"Fermentation."},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/7086047","article-title":"Optimization of Bioethanol Production Using Response Surface Methodology for Stress-Tolerant Wild Yeasts Isolated from Natural Forests.","volume":"2024","author":"T Tadesse","year":"2024","journal-title":"Int. J. Energy Res."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"1-15","DOI":"10.3390\/microorganisms7120633","article-title":"Occurrence and molecular identification of wild yeasts from Jimma Zone, South West Ethiopia.","volume":"7","author":"A Koricha","year":"2019","journal-title":"Microorganisms."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"913-921","DOI":"10.2147\/IDR.S352307","article-title":"Comparison of Autof Ms1000 and EXS3000 MALDI-TOF MS Platforms for Routine Identification of Microorganisms.","volume":"16","author":"L Xiong","year":"2023","journal-title":"Infect. Drug Resist."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"474-481","DOI":"10.1134\/S0026261708040140","article-title":"Characterization of yeast groupings in the phyllosphere of Sphagnum mosses.","volume":"77","author":"A Kachalkin","year":"2008","journal-title":"Microbiology."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"73-100","DOI":"10.1007\/978-3-319-61575-2","article-title":"Yeast Community Composition and Structure.","author":"A Yurkov","year":"2017"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"24-35","DOI":"10.1016\/j.funeco.2011.07.004","article-title":"Assessment of yeast diversity in soils under different management regimes.","volume":"5","author":"A Yurkov","year":"2011","journal-title":"Fungal Ecol."},{"key":"ref19","first-page":"1-18","article-title":"Patterns of yeast diversity distribution and its drivers in rhizosphere soil of Hami melon orchards in different regions of Xinjiang.","author":"S Zhu","year":"2021"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"489-494","DOI":"10.1007\/s00284-008-9108-x","article-title":"Yeasts and Yeast-Like Fungi Associated with Tree Bark: Diversity and Identification of Yeasts Producing Extracellular Endoxylanases.","author":"S Bhadra","year":"2008"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"97-103","DOI":"10.21660\/2019.61.4796","article-title":"Stress tolerance Yeast strain from papaya wastes for bioethanol production.","volume":"17","author":"G Utama","year":"2019","journal-title":"Int. J. GEOMATE."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"811-827","DOI":"10.1042\/EBC20220233","article-title":"Evolution and functional diversification of yeast sugar transporters.","volume":"67","author":"L Donzella","year":"2023","journal-title":"Essays Biochem."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"229-242","DOI":"10.1016\/0378-1097(93)90598-V","article-title":"Sugar transport in Saccharomyces cerevisiae.","volume":"104","author":"R Lagunas","year":"1993","journal-title":"FEMS Microbiol. Lett."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"390-399","DOI":"10.1016\/j.bcab.2018.09.006","article-title":"Biochemical evaluation, molecular characterization and identification of novel yeast strains isolated from Brazilian savannah fruits, chicken litter and a sugar and alcohol mill with biotechnological potential for biofuel and food industries.","volume":"16","author":"J Camargo","year":"2018","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"3828-3833","DOI":"10.1021\/jf400463r","article-title":"A novel approach for the quantitation of carbohydrates in mash, wort, and beer with RP-HPLC using 1-naphthylamine for precolumn derivatization.","volume":"61","author":"S Rakete","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"7176-7182","DOI":"10.1128\/AEM.01704-06","article-title":"Fermentation of high concentrations of maltose by Saccharomyces cerevisiae is limited by the compass methylation complex.","volume":"72","author":"J Houghton-Larsen","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref27","first-page":"325-334","article-title":"Isolation of xylose-assimilating yeasts and optimization of xylitol production by a new Meyerozyma guilliermondii strain.","author":"F Augusto","year":"2020"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"e43135","DOI":"10.1371\/journal.pone.0043135","article-title":"Diversity and physiological characterization of D-xylose-fermenting yeasts isolated from the Brazilian Amazonian Forest.","volume":"7","author":"R Cadete","year":"2012","journal-title":"PLoS One."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"77-100","DOI":"10.1016\/b978-044481312-1\/50014-9","article-title":"Methods for the isolation, maintenance and identification of yeasts.","author":"D Yarrow","year":"1998","journal-title":"The Yeasts."},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.21203\/rs.3.rs-138293\/v1","article-title":"Patterns of Yeast Diversity Distribution and its Drivers in Rhizosphere Soil of Hami Melon Orchards in Different Regions of Xinjiang.","author":"S Zhu","year":"2021"},{"key":"ref31","first-page":"226-232","article-title":"Seasonal dynamics in a yeast population on the common wood sorrel Oxalis acetosella L. leaves.","volume":"73","author":"A Glushakova","year":"2004","journal-title":"Mikrobiologiya."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"430-436","DOI":"10.1002\/jobm.200310277","article-title":"The diversity of yeasts in the agricultural soil.","volume":"43","author":"E Sl\u00e1vikov\u00e1","year":"2003","journal-title":"J. Basic Microbiol."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"012012","DOI":"10.1088\/1755-1315\/368\/1\/012012","article-title":"Distribution features of yeasts in soils of South Vietnam (case study of the biogeocenoses of the National Park C\u00e1t Tien).","volume":"368","author":"A Glushakova","year":"2019","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"19-24","DOI":"10.1111\/j.1472-765X.2008.02380.x","article-title":"Isolation and characterization of ethanol-producing yeasts from fruits and tree barks.","volume":"47","author":"R Rao","year":"2008","journal-title":"Lett. Appl. Microbiol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"303-314","DOI":"10.1111\/jam.12807","article-title":"Production of arabitol by yeasts: Current status and future prospects.","volume":"119","author":"M Kordowska-Wiater","year":"2015","journal-title":"J. Appl. Microbiol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"2917-2930","DOI":"10.1111\/mec.12155","article-title":"Mixing of vineyard and oak-tree ecotypes of Saccharomyces cerevisiae in North American vineyards.","volume":"22","author":"K Hyma","year":"2013","journal-title":"Mol. Ecol."},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-52149-1.00007-0","author":"C Kurtzman","year":"2011","journal-title":"Methods for isolation, phenotypic characterization and maintenance of yeasts."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"575-594","DOI":"10.3934\/microbiol.2022037","article-title":"Diversity of fermentative yeasts with probiotic potential isolated from Thai fermented food products.","volume":"8","author":"S Punyauppa-Path","year":"2022","journal-title":"AIMS Microbiol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"1-17","DOI":"10.3390\/microorganisms8071086","article-title":"Functional biodiversity of yeasts isolated from Colombian fermented and dry Cocoa beans.","volume":"8","author":"J Delgado-Ospina","year":"2020","journal-title":"Microorganisms."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"75-83","DOI":"10.1111\/lam.12514","article-title":"High temperature alcoholic fermentation of orange peel by the newly isolated thermotolerant Pichia kudriavzevii KVMP10.","volume":"62","author":"M Koutinas","year":"2016","journal-title":"Lett. Appl. Microbiol."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"461-475","DOI":"10.1016\/j.bjm.2017.01.006","article-title":"High-temperature ethanol production using thermotolerant yeast newly isolated from Greater Mekong Subregion, Brazilian.","volume":"48","author":"A Techaparin","year":"2017","journal-title":"J. Microbiol."},{"key":"ref43","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.908628","article-title":"Genetic, Phenotypic and Metabolic Diversity of Yeasts From Wheat Flag Leaves.","volume":"13","author":"L Gouka","year":"2022"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"478-491","DOI":"10.1016\/B978-012373944-5.00335-7","article-title":"Yeasts.","author":"G Walker","year":"2009","journal-title":"Encycl. Microbiol."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"617-631","DOI":"10.1002\/yea.3430","article-title":"Diversity of yeasts in the soil adjacent to fruit trees of the Rosaceae family.","volume":"36","author":"R Vadkertiov\u00e1","year":"2019","journal-title":"Yeast."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"10-86","DOI":"10.1186\/s13068-021-01940-y","article-title":"Bioethanolic yeasts from dung beetles: tapping the potential of extremophilic yeasts for improvement of lignocellulolytic feedstock fermentation.","volume":"14","author":"A Nwaefuna","year":"2021","journal-title":"Biotechnol. Biofuels."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"1236-1250","DOI":"10.1002\/ece3.1919","article-title":"Summer temperature can predict the distribution of wild yeast populations.","volume":"6","author":"H Robinson","year":"2016","journal-title":"Ecol. Evol."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"155-157","DOI":"10.1007\/s12223-013-0278-y","article-title":"First evidence for seasonal fluctuations in lichen- and bark-colonising fungal communities.","volume":"59","author":"A Beck","year":"2014","journal-title":"Folia Microbiol. (Praha)."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"845-859","DOI":"10.1007\/s11557-016-1206-8","article-title":"Yeast Diversity and Species Recovery Rates from Beech Forest Soils.","volume":"15","author":"A Yurkov","year":"2016","journal-title":"Mycol. Prog."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"216-219","DOI":"10.1186\/s13068-017-0899-5","article-title":"Phenotypic landscape of non-conventional yeast species for different stress tolerance traits desirable in bioethanol fermentation.","volume":"10","author":"V Mukherjee","year":"2017","journal-title":"Biotechnol. Biofuels."},{"key":"ref51","first-page":"1-19","article-title":"Isolation of wild yeasts from Olympic National Park and Moniliella megachiliensis ONP131 physiological characterization for beer fermentation Authors.","author":"R Piraine","year":"2021"},{"key":"ref52","doi-asserted-by":"publisher","first-page":"109-130","DOI":"10.1093\/femsre\/fuw040","article-title":"Forest microbiome: Diversity, complexity and dynamics.","volume":"41","author":"P Baldrian","year":"2017","journal-title":"FEMS Microbiol. Rev."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"1-12","DOI":"10.1016\/j.funeco.2017.12.005","article-title":"Decaying Picea abies log bark hosts diverse fungal communities.","volume":"33","author":"I Kazartsev","year":"2018","journal-title":"Fungal Ecol."},{"key":"ref54","article-title":"How plants communicate with their biotic environment.","author":"P Lemanceau","year":"2017"},{"key":"ref55","doi-asserted-by":"publisher","first-page":"309-321","DOI":"10.1002\/yea","article-title":"Osmotolerant yeast species differ in basic physiological parameters and in tolerance of non-osmotic stresses.","volume":"31","author":"M Bubnov\u00e1","year":"2014","journal-title":"Yeast."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"179-187","DOI":"10.1016\/J.IJFOODMICRO.2009.01.024","article-title":"Molecular identification and osmotolerant profile of wine yeasts that ferment a high sugar grape must.","volume":"130","author":"R Tofalo","year":"2009","journal-title":"Int. J. Food Microbiol."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"1875-1883","DOI":"10.1128\/AEM.69.4.1875-1883.2003","article-title":"Microbiology of the phyllosphere.","volume":"69","author":"S Lindow","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"1344-1352","DOI":"10.1139\/cjm-2012-0468","article-title":"Yeasts and yeast-like organisms associated with fruits and blossoms of different fruit trees.","volume":"58","author":"R Vadkertiov\u00e1","year":"2012","journal-title":"Can. J. Microbiol."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"9483-9498","DOI":"10.1007\/s00253-014-6090-z","article-title":"Phenotypic evaluation of natural and industrial Saccharomyces yeasts for different traits desirable in industrial bioethanol production.","volume":"98","author":"V Mukherjee","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref60","doi-asserted-by":"publisher","DOI":"10.6084\/m9.figshare.28078370","author":"T Tadesse","year":"2024","journal-title":"figshare."}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/14-105\/v3\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/14-105\/v3\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/14-105\/v3\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T08:20:08Z","timestamp":1745655608000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/14-105\/v3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,8]]},"references-count":59,"URL":"https:\/\/doi.org\/10.12688\/f1000research.160250.3","relation":{"has-version":[{"id-type":"doi","id":"10.12688\/f1000research.160250.2","asserted-by":"subject"},{"id-type":"doi","id":"10.12688\/f1000research.160250.1","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.179817.r376733","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.179817.r376733","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,8]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/14-105\/v3#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.179817.r376733, Pablo Villarreal, Facultad de Qu\u00edmica y Biolog\u00eda, Departamento de Biolog\u00eda, University of Santiago Chile, Santiago, Santiago Metropolitan Region, 26 Apr 2025, version 3, indexed","URL":"https:\/\/f1000research.com\/articles\/14-105\/v3#referee-response-376733","order":0,"name":"referee-response-376733","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.176114.r362747, Lydia R Heasley, Colorado State University, Fort Collins, Colorado, 14 Feb 2025, version 1, 1 approved, 1 not approved","URL":"https:\/\/f1000research.com\/articles\/14-105\/v1#referee-response-362747","order":1,"name":"referee-response-362747","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Teshome Tadesse<\/b>; \n<i>Posted: 27 Feb 2025<\/i>; We thank the anonymous reviewer for the very constructive and positive comments.  Reviewer\u2019s Suggestion: The importance of adding some pictures to enhance the readability of the manuscript:  Author Response:  Some pictures of the yeast isolates (morphology) as seen under the microscope and on solid media are included (Figures, 2 and 4). The same figures were incorporated into the manuscript.&nbsp;&nbsp;&nbsp;","URL":"https:\/\/f1000research.com\/articles\/14-105\/v3#referee-comment-13432","order":2,"name":"referee-comment-13432","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"Facilities and financial support for this project are provided by Jimma University, College of Natural Sciences Research Fund (Grant number RPG\/183-2020). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":3,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}