{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:08:15Z","timestamp":1772168895409,"version":"3.50.1"},"reference-count":46,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T00:00:00Z","timestamp":1738195200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    Objective To evaluate the effect of local application of Sodium Alendronate incorporated in Carbapol gel together with LLLT on peri-implant tissue healing in Wistar rat femurs. Methods Twenty-four male Wistar rats were randomly divided into 4 groups: Group 1 (the control group), Group 2 (the Sodium Alendronate group), Group 3 (the Sodium Alendronate and LLLT group), and Group 4 (the LLLT group). Mini implants were placed in right and left femur bones in all the four groups. Implants of groups 2 and 3 were coated in 1mg Carbapol gel incorporated with 1 mg Sodium alendronate. Groups 3 and 4 were exposed to LLLT (CO2 laser, wavelength 830nm; 2.1J\/cm\n                    <ns4:sup>2<\/ns4:sup>\n                    ) on 1\n                    <ns4:sup>st,<\/ns4:sup>\n                    7\n                    <ns4:sup>th<\/ns4:sup>\n                    , 14\n                    <ns4:sup>th<\/ns4:sup>\n                    and 21\n                    <ns4:sup>st<\/ns4:sup>\n                    day. Animals were sacrificed on the 28th day, following which the femurs were dissected out and stored in 10% buffered formaldehyde for histopathological analysis. Results Groups 3 and 4 showed bony union and formation of reorganized spongiosa whereas Groups 1 and 2 showed fibrous union. The bone marrow from Group 3 had an adult-type fatty marrow, while that from Group 2 and 4 was occupied by red blood cells. Group 1 showed initial stages of bone healing in which the defect occupied more than half of the bone marrow. Conclusion LLLT given using CO2 laser therapy, together with a one-time application of Sodium Alendronate in Carbapol gel at the time of implant placement optimally enhances healing of peri-implant tissues.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.160479.1","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T11:35:10Z","timestamp":1738236910000},"page":"147","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A Randomized Controlled Trial Evaluating the Effect of Local Application of Sodium Alendronate Gel and Low-Level Laser Therapy (LLLT) On Peri-Implant Tissue Healing in Wistar Rats"],"prefix":"10.12688","volume":"14","author":[{"given":"Divya","family":"Mishra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arun S","family":"Urala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8029-6002","authenticated-orcid":false,"given":"Ashwath S","family":"Nayak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8957-8914","authenticated-orcid":false,"given":"Divya","family":"S","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajay","family":"Kamath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1828-6946","authenticated-orcid":false,"given":"Lakshmi Narayan","family":"Bairy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gargi","family":"S","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2025,1,30]]},"reference":[{"key":"ref2","doi-asserted-by":"publisher","first-page":"S43-S51","DOI":"10.1016\/j.ajodo.2006.04.027","article-title":"Risks and complications of orthodontic miniscrews.","volume":"131","author":"N Kravitz","year":"2007 Apr","journal-title":"Am. J. Orthod. Dentofacial Orthop."},{"key":"ref3","first-page":"63-69","article-title":"Peri-implantitis.","volume":"9","author":"M Sanchez-Garces","year":"2004","journal-title":"Med. Oral Patol. Oral Cir. Bucal."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"10-15","DOI":"10.1053\/j.sodo.2004.11.003","article-title":"Intraoral hard and soft tissue depths for temporary anchorage devices.","volume":"11","author":"A Costa","year":"2005","journal-title":"Semin. Orthod."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"361-366","DOI":"10.3109\/00016358909004804","article-title":"The effect of chlorhexidine supplementation in a periodontal dressing.","volume":"47","author":"S Othman","year":"1989 Dec","journal-title":"Acta Odontol. Scand."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"845-849","DOI":"10.1111\/j.1600-051x.2004.00567.x","article-title":"Spontaneous progression of experimentally induced periimplantitis.","volume":"31","author":"N Zitzmann","year":"2004 Oct","journal-title":"J. Clin. Periodontol."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"99-103","DOI":"10.1034\/j.1600-0501.1992.030301.x","article-title":"Long-standing plaque and gingivitis at implants and teeth in the dog.","volume":"3","author":"I Ericsson","year":"1992 Sep","journal-title":"Clin. Oral Implants Res."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"257-265","DOI":"10.1007\/s10103-013-1319-0","article-title":"Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice.","volume":"29","author":"A Gupta","year":"2014 Jan","journal-title":"Lasers Med. Sci."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"271-277","DOI":"10.1089\/104454703322564479","article-title":"Effects of pulse frequency of low-level laser therapy (LLLT) on bone nodule formation in rat calvarial cells.","volume":"21","author":"Y Ueda","year":"2003 Oct","journal-title":"J. Clin. Laser Med. Surg."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"288-292","DOI":"10.1034\/j.1600-0501.2002.130308.x","article-title":"Effect of low-power laser irradiation on bony implant sites.","volume":"13","author":"O D\u00f6rtbudak","year":"2002 Jun","journal-title":"Clin. Oral Implants Res."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"411-416","DOI":"10.1089\/pho.2008.2478","article-title":"Effect of biostimulation on healing of bone defects in diabetic rats.","volume":"28","author":"U Akyol","year":"2010 Jun","journal-title":"Photomed. Laser Surg."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"24-33","DOI":"10.1007\/s00774-007-0782-8","article-title":"Alendronate treatment promotes bone formation with a less anisotropic microstructure during intramembranous ossification in rats.","volume":"26","author":"M Kashii","year":"2008","journal-title":"J. Bone Miner. Metab."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"6941-6949","DOI":"10.1016\/j.biomaterials.2005.04.059","article-title":"Effects of bisphosphonates on proliferation and osteoblast differentiation of human bone marrow stromal cells.","volume":"26","author":"F Knoch","year":"2005 Dec","journal-title":"Biomaterials."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"733-759","DOI":"10.1007\/s00198-007-0540-8","article-title":"Mechanisms of action of bisphosphonates: similarities and differences and their potential influence on clinical efficacy.","volume":"19","author":"R Russell","year":"2008 Jun","journal-title":"Osteoporos Int."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"2345-2352","DOI":"10.1007\/s00198-013-2332-7","article-title":"Effectiveness of risedronate and alendronate on nonvertebral fractures: an observational study through 2 years of therapy.","volume":"24","author":"R Lindsay","year":"2013 Aug","journal-title":"Osteoporos Int."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"2042-2049","DOI":"10.1016\/j.joms.2008.06.023","article-title":"Effects of alendronate on rate of distraction in rabbit mandibles.","volume":"66","author":"U Tekin","year":"2008 Oct","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"75-85","DOI":"10.1016\/8756-3282(95)00445-9","article-title":"Bisphosphonates: a review of their pharmacokinetic properties.","volume":"18","author":"J Lin","year":"1996 Feb","journal-title":"Bone."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"984-988","DOI":"10.1302\/0301-620x.89b7.18980","article-title":"Locally administered low-dose alendronate increases bone mineral density during distraction osteogenesis in a rabbit model.","volume":"89-B","author":"H Omi","year":"2007 Jul","journal-title":"J. Bone Joint Surg. Br."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"1141-1146","DOI":"10.1007\/s10103-015-1720-y","article-title":"The influence of low-level laser therapy with alendronate irrigation on healing of bone defects in rats.","volume":"30","author":"U Akyol","year":"2015 Apr","journal-title":"Lasers Med. Sci."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1201\/9781584889830","author":"S Chow","year":"2008","journal-title":"Sample Size Calculations in Clinical Research."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"379-390","DOI":"10.1016\/j.biopha.2018.02.052","article-title":"Plumbagin ameliorates memory dysfunction in streptozotocin induced Alzheimer\u2019s disease via activation of Nrf2\/ARE pathway and inhibition of \u03b2-secretase.","volume":"101","author":"K Nakhate","year":"2018 May","journal-title":"Biomed. Pharmacother."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"72-80","DOI":"10.1016\/j.lfs.2013.12.014","article-title":"Involvement of the central melanocortin system in the effects of caffeine on anxiety-like behavior in mice.","volume":"95","author":"A Bhorkar","year":"2014 Jan 30","journal-title":"Life Sci."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"246-250","DOI":"10.1016\/j.vascn.2011.08.002","article-title":"A simple and inexpensive method to fabricate a cannula system for intracranial injections in rats and mice.","volume":"64","author":"D Kokare","year":"2011 Nov-Dec","journal-title":"J. Pharmacol. Toxicol. Methods."},{"key":"ref24","first-page":"73-79","article-title":"Proposal for access to the femur in rats.","volume":"2","author":"C Costa","year":"2011","journal-title":"Int. J. Biotechnol. Mol. Biol. Res."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"179-185","DOI":"10.1016\/S0030-5898(20)30381-3","article-title":"Biology of cancellous bone grafts.","volume":"18","author":"K Heiple","year":"1987 Apr","journal-title":"Orthop. Clin. North Am."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"18-25","DOI":"10.1016\/j.ajodo.2004.11.032","article-title":"Factors affecting the clinical success of screw implants used as orthodontic anchorage.","volume":"130","author":"H Park","year":"2006 Jul","journal-title":"Am. J. Orthod. Dentofacial Orthop."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"1423-1429","DOI":"10.1016\/j.ijom.2015.05.013","article-title":"Influence of the local application of sodium alendronate gel on osseointegration of titanium implants.","volume":"44","author":"M Guimar\u00e3es","year":"2015 Nov","journal-title":"Int. J. Oral Maxillofac. Surg."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"204-211","DOI":"10.1002\/lsm.1039","article-title":"Attenuation of infarct size in rats and dogs after myocardial infarction by low-energy laser irradiation.","volume":"28","author":"U Oron","year":"2001","journal-title":"Lasers Surg. Med."},{"key":"ref29","first-page":"61-77","article-title":"Laser stimulation of collagen synthesis in human skin fibroblast cultures.","volume":"1","author":"T Lam","year":"1989","journal-title":"Laser Life Sci."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"157-162","DOI":"10.1159\/000249691","article-title":"In vitro fibroblast and dermis fibroblast activation by laser irradiation at low energy. An electron microscopic study.","volume":"168","author":"M Bosatra","year":"1984","journal-title":"Dermatologica."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"297-300","DOI":"10.1007\/s002239900126","article-title":"Promotion of bone repair in the cortical bone of the tibia in rats by low energy laser (He-Ne) irradiation.","volume":"59","author":"T Yaakobi","year":"1996 Oct","journal-title":"Calcif. Tissue Int."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"191-199","DOI":"10.1002\/lsm.1900150207","article-title":"Effect of low-intensity argon laser irradiation on mitochondrial respiration.","volume":"15","author":"Y Morimoto","year":"1994","journal-title":"Lasers Surg. Med."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"866-871","DOI":"10.1111\/j.1751-1097.1997.tb03239.x","article-title":"Photomodulation of oxidative metabolism and electron chain enzymes in rat liver mitochondria.","volume":"66","author":"W Yu","year":"1997 Dec","journal-title":"Photochem. Photobiol."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"3503-3509","DOI":"10.1016\/j.biomaterials.2004.09.033","article-title":"Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material.","volume":"26","author":"M Khadra","year":"2005 Jun","journal-title":"Biomaterials."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"788-796","DOI":"10.1002\/lsm.20585","article-title":"Effect of low-level laser therapy on bone repair: histological study in rats.","volume":"39","author":"H Pretel","year":"2007 Dec","journal-title":"Lasers Surg. Med."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"884-891","DOI":"10.1016\/j.archoralbio.2012.01.009","article-title":"Evaluation of socket healing in irradiated rats after diode laser exposure (histological and morphometric studies).","volume":"57","author":"N Korany","year":"2012 Jul","journal-title":"Arch. Oral Biol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"693-700","DOI":"10.1016\/j.tripleo.2003.11.008","article-title":"Enhancement of bone formation in rat calvarial bone defects using low-level laser therapy.","volume":"97","author":"M Khadra","year":"2004 Jun","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"301-306","DOI":"10.1089\/104454703322564523","article-title":"Effect of 830-nm laser light on the repair of bone defects grafted with inorganic bovine bone and decalcified cortical osseous membrane.","volume":"21","author":"A Pinheiro","year":"2003 Oct","journal-title":"J. Clin. Laser Med. Surg."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"181-183","DOI":"10.1089\/clm.1997.15.181","article-title":"Low-level laser therapy in the management of disorders of the maxillofacial region.","volume":"15","author":"A Pinheiro","year":"1997","journal-title":"J. Clin. Laser Med. Surg."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"532-535","DOI":"10.1016\/0002-9610(71)90482-x","article-title":"Effect of laser rays on wound healing.","volume":"122","author":"E Mester","year":"1971 Oct","journal-title":"Am. J. Surg."},{"key":"ref41","first-page":"347-356","article-title":"Stimulation of wound healing by means of laser rays. (Clinical and electron microscopical study).","volume":"14","author":"E Mester","year":"1973","journal-title":"Acta Chir. Acad. Sci. Hung."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"50-58","DOI":"10.1002\/lsm.1900090111","article-title":"Effect of helium-neon and infrared laser irradiation on wound healing in rabbits.","volume":"9","author":"B Braverman","year":"1989","journal-title":"Lasers Surg. Med."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"281-287","DOI":"10.1002\/(sici)1096-9101(1998)22:5%3C281::aid-lsm4%3E3.0.co;2-l","article-title":"Laser photostimulation of collagen production in healing rabbit Achilles tendons.","volume":"22","author":"G Reddy","year":"1998","journal-title":"Lasers Surg. Med."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"56-63","DOI":"10.1002\/(sici)1096-9101(1997)20:1%3C56::aid-lsm9%3E3.0.co;2-y","article-title":"Effects of photostimulation on wound healing in diabetic mice.","volume":"20","author":"W Yu","year":"1997","journal-title":"Lasers Surg. Med."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"231-235","DOI":"10.1002\/(sici)1096-9101(1999)24:3%3C231::aid-lsm8%3E3.0.co;2-#","article-title":"Penetration of the laser light into the skin in vitro.","volume":"24","author":"H Kol\u00e1rov\u00e1","year":"1999","journal-title":"Lasers Surg. Med."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"331-342","DOI":"10.1002\/lsm.1900160404","article-title":"Low intensity laser therapy: still not an established clinical tool.","volume":"16","author":"J Basford","year":"1995","journal-title":"Lasers Surg. Med."},{"key":"ref47","doi-asserted-by":"publisher","author":"S Divya","year":"2024","DOI":"10.6084\/m9.figshare.28093763"}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/14-147\/v1\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/14-147\/v1\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/14-147\/v1\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T04:20:21Z","timestamp":1740630021000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/14-147\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,30]]},"references-count":46,"URL":"https:\/\/doi.org\/10.12688\/f1000research.160479.1","relation":{"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.176384.r364233","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.176384.r364236","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.176384.r364236","asserted-by":"object"},{"id-type":"doi","id":"10.5256\/f1000research.176384.r364233","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,30]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/14-147\/v1#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.176384.r364236, Goutham B, Department of Orthodontics and Dentofacial Orthopaedics, Coorg Institute of Dental Sciences, Virajpet, Karnataka, 12 Feb 2025, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/14-147\/v1#referee-response-364236","order":0,"name":"referee-response-364236","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.176384.r364233, Veerasathpurush Allareddy, Department of Orthodontics, University of Illinois Chicago, Chicago, Illinois, 27 Feb 2025, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/14-147\/v1#referee-response-364233","order":1,"name":"referee-response-364233","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"The author(s) declared that no grants were involved in supporting this work","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}