{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:07:56Z","timestamp":1772168876546,"version":"3.50.1"},"update-to":[{"DOI":"10.12688\/f1000research.164673.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:00:00Z","timestamp":1760054400000}},{"DOI":"10.12688\/f1000research.164673.2","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:00:00Z","timestamp":1760054400000}}],"reference-count":40,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:00:00Z","timestamp":1760054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad De San Mart\u00edn de Porres","award":["E21102023008"],"award-info":[{"award-number":["E21102023008"]}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>\n                    Introduction Diabetes mellitus (DM) is a chronic metabolic disease representing a global public health concern and is associated with severe complications such as cardiovascular and renal diseases. Although several species of the genus\n                    <ns3:italic>Acalypha<\/ns3:italic>\n                    have demonstrated biological activity, no prior studies have evaluated the hypoglycemic effect of\n                    <ns3:italic>Acalypha argomuelleri<\/ns3:italic>\n                    Briq., making this study relevant. Method The hypoglycemic effect of the aqueous leaf extract of\n                    <ns3:italic>A. argomuelleri<\/ns3:italic>\n                    Briq. (AAAE) was evaluated in an experimental model using\n                    <ns3:italic>Rattus r<\/ns3:italic>\n                    attus var. albinus (males). A randomized, prospective design was employed, consisting of a control group and three treatment groups receiving doses of 100, 150, and 300 mg\/kg of the extract, respectively. Hyperglycemia was induced via oral glucose administration. Results The qualitative phytochemical analysis of AAAE revealed the presence of flavonoids, phenols, cardiotonic glycosides, and diterpenes, with no reducing sugars. The 300 mg\/kg dose produced a significant and sustained reduction in blood glucose levels, reaching near-normal values at 90 minutes, demonstrating a dose- and time-dependent hypoglycemic effect. Discussion The study confirmed that AAAE has a dose-dependent hypoglycemic effect, with optimal efficacy at 300 mg\/kg. This dose showed a faster and more sustained reduction in glucose levels compared to 100 and 150 mg\/kg, suggesting higher efficacy at elevated concentrations. The identified flavonoids and phenols, associated with glucose metabolism modulation and pancreatic \u03b2-cell protection, likely explain the observed effect. The absence of reducing sugars indicates the hypoglycemic effect is linked to secondary metabolites. Conclusions The AAAE exhibited a significant dose- and time-dependent hypoglycemic effect, with optimal efficacy at 300 mg\/kg after 90 minutes. These findings support the potential of\n                    <ns3:italic>A. argomuelleri<\/ns3:italic>\n                    Briq. as a natural alternative for blood glucose control, though further studies are needed to assess its safety and efficacy in clinical models.\n                  <\/ns3:p>","DOI":"10.12688\/f1000research.164673.3","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:10:07Z","timestamp":1760112607000},"page":"800","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Hypoglycemic effect of the aqueous extract from Acalypha argomuelleri Briq. (Euphorbiaceae) 'Sweet stick' leaves in Rattus rattus var. albinus"],"prefix":"10.12688","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0012-1817","authenticated-orcid":false,"given":"Jorge Guillermo","family":"Morales Ramos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Berta","family":"Loja Herrera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00e9stor Manuel","family":"Rodr\u00edguez Alayo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doyle Isabel","family":"Benel Fern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Felipe","family":"P\u00e9rez Medina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7699-8514","authenticated-orcid":false,"given":"Carolina Susana","family":"Loayza Estrada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Ema Soledad","family":"Mocarro Willis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar","family":"S\u00e1nchez Mar\u00edn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2025,10,10]]},"reference":[{"key":"ref2","doi-asserted-by":"publisher","first-page":"2077-2093","DOI":"10.1016\/S0140-6736(24)02317-1","article-title":"Worldwide trends in diabetes prevalence and treatment from 1990 to 2022: a pooled analysis of 1108 population-representative studies with 141 million participants.","volume":"404","author":"B Zhou","year":"Nov. 2024","journal-title":"Lancet."},{"key":"ref4","article-title":"IDF Diabetes Atlas 2021, 10th Edition Committee.","author":"D Magliano","journal-title":"Online version of IDF Diabetes Atlas."},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.3139","author":"R Carrillo-Larco","year":"Mar. 04, 2019","journal-title":"The risk of mortality among people with type 2 diabetes in Latin America: A systematic review and meta-analysis of population-based cohort studies."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"509-511","DOI":"10.1016\/J.MEDCLE.2024.05.020","article-title":"Has the first treatment of type 2 diabetes mellitus changed?","volume":"163","author":"L Flores","year":"Nov. 2024","journal-title":"Medicina Cl\u00ednica (English Edition)."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"3709","DOI":"10.3390\/NU16213709","article-title":"Plant-Based Diets and Phytochemicals in the Management of Diabetes Mellitus and Prevention of Its Complications: A Review.","volume":"16","author":"P Ansari","year":"Oct. 2024","journal-title":"Nutrients."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"1022","DOI":"10.12688\/f1000research.55573.1","article-title":"Medicinal properties of \u2018Morus alba\u2019 for the control of type 2 diabetes mellitus: a systematic review.","volume":"10","author":"J Morales Ramos","year":"Oct. 2021","journal-title":"F1000Res."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"50-62","DOI":"10.1016\/J.EJPHAR.2018.05.021","article-title":"Analysis of mulberry leaf components in the treatment of diabetes using network pharmacology.","volume":"833","author":"Q Ge","year":"Aug. 2018","journal-title":"Eur. J. Pharmacol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"17-30","DOI":"10.3724\/SP.J.1009.2016.00017","article-title":"Phytochemistry, pharmacology, and clinical trials of Morus alba.","volume":"14","author":"E Chan","year":"Jan. 2016","journal-title":"Chin. J. Nat. Med."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"1441-1447","DOI":"10.13005\/BBRA\/2589","article-title":"Effect of Morus alba against Hyperglycemic and Hyperlipidemic Activities in Streptozotocin Induced Diabetic Nephropathy.","volume":"14","author":"P Swathi","year":"Dec. 2017","journal-title":"Biosci. Biotechnol. Res. Asia."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"159-163","DOI":"10.31254\/PHYTO.2022.11305","article-title":"Phytochemical screening and effects of aqueous extract of Acalypha wilkesiana M\u00fcll. Arg on isolated toad heart.","volume":"11","author":"W Mendame","year":"Jun. 2022","journal-title":"J. Phytopharmacol."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"e34726","DOI":"10.5812\/IJEM.34726","article-title":"Chemical Composition and the Effect of Walnut Hydrosol on Glycemic Control of Patients With Type 1 Diabetes.","volume":"14","author":"H Moravej","year":"Jan. 2016","journal-title":"Int. J. Endocrinol. Metab."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1186\/S12906-018-2268-8","article-title":"Effects of a hydroalcoholic extract of Juglans regia (walnut) leaves on blood glucose and major cardiovascular risk factors in type 2 diabetic patients: a double-blind, placebo-controlled clinical trial.","volume":"18","author":"K Rabiei","year":"Jul. 2018","journal-title":"BMC Complement. Altern. Med."},{"key":"ref15","first-page":"8-13","article-title":"Actividad antibacterial de extractos de Nogal Juglans regia.","volume":"18","author":"P Mar\u00eda","year":"Jul. 2023","journal-title":"Revista de Investigaci\u00f3n en Ciencias de la Salud."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"88-93","DOI":"10.18633\/BIOTECNIA.V25I1.1773","article-title":"Actividad antioxidante, t\u00f3xica y antimicrobiana de Rosmarinus officinalis, Ruta graveolens y Juglans regia contra Helicobacter pylori.","volume":"25","author":"J Perales-Flores","year":"Nov. 2023","journal-title":"Biotecnia."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"15-24","DOI":"10.55996\/DEKAMUAGROPEC.V2I1.46","article-title":"Efecto hipoglucemiante de las hojas y flores de Geranium ayavacense L. \u2018pasuchaca\u2019 sobre la glicemia en Rattus rattus var. Albinus.","volume":"2","author":"L Gonzales Llontop","year":"Sep. 2021","journal-title":"Revista Cient\u00edfica D\u00e9kamu Agropec."},{"key":"ref18","first-page":"40-45","article-title":"Efecto del extracto hidroetan\u00f3lico de geranium ruizii hieron sobre la hiperglicemia inducida en rattus rattus variedad albinus.","volume":"48","author":"J Diaz Ortega","year":"Jan. 2023","journal-title":"Interciencia: Revista de ciencia y tecnolog\u00eda de Am\u00e9rica."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"12-16","DOI":"10.26722\/RPMI.2016.12.14","article-title":"Efecto hipoglicemiante del infuso de planta total de Psoralea glandulosa \u2018cullen\u2019 En rattus Var albinus normoglic\u00e9micas.","volume":"1","author":"J Ram\u00edrez Ver\u00e1stegui","year":"Aug. 2016","journal-title":"Revista Peruana de Medicina Integrativa."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"87-98","DOI":"10.47606\/ACVEN\/MV0135","article-title":"Propiedades beneficiosas del yac\u00f3n (smallanthus sonchifolius) en la salud.","volume":"4","author":"K Minchola-Casta\u00f1eda","year":"Sep. 2022","journal-title":"M\u00e1s Vita."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"53-59","DOI":"10.1016\/J.HERMED.2017.03.005","article-title":"Antibiotic-potentiating activity, phytochemical profile, and cytotoxicity of Acalypha integrifolia Willd. (Euphorbiaceae).","volume":"11","author":"R Seebaluck-Sandoram","year":"Mar. 2018","journal-title":"J. Herb. Med."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"74-74","DOI":"10.21010\/AJIDV17I1.7","article-title":"ANTI-FUNGAL ACTIVITY OF ACALYPHA WILKESIANA: A PRELIMINARY STUDY OF FUNGAL ISOLATES OF CLINICAL SIGNIFICANCE.","volume":"17","author":"K Sherifat","year":"Dec. 2023","journal-title":"Afr. J. Infect. Dis."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"104-109","DOI":"10.1016\/J.JSPS.2016.05.004","article-title":"Bioactivity and chemical characterization of Acalypha fruticosa Forssk. growing in Saudi Arabia.","volume":"25","author":"G Fawzy","year":"Jan. 2017","journal-title":"Saudi Pharm. J."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"2326","DOI":"10.3390\/PLANTS10112326","article-title":"Cytotoxic Effect in vitro of Acalypha monostachya Extracts over Human Tumor Cell Lines.","volume":"10","author":"G Guill\u00e9n-Mel\u00e9ndez","year":"Oct. 2021","journal-title":"Plants."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"477-497","DOI":"10.1600\/036364422X16512572275034","article-title":"Phylogeny, Classification, and Character Evolution of Acalypha (Euphorbiaceae: Acalyphoideae).","volume":"47","author":"G Levin","year":"Jun. 2022","journal-title":"Syst. Bot."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"151-168","DOI":"10.18387\/POLIBOTANICA.48.12","article-title":"An\u00e1lisis fitoqu\u00edmico cualitativo de los extractos acuosos de Thalassia testudinum Banks ex K\u00f6ning et Sims de la localidad de Champot\u00f3n, Campeche, M\u00e9xico, durante el ciclo anual 2016-2017.","author":"R Garc\u00eda-Granados","year":"Jul. 2019","journal-title":"Polibotanica."},{"key":"ref28","first-page":"406-419","article-title":"Estudio fitoqu\u00edmico de la especie end\u00e9mica cubana Zanthoxylum pseudodumosum, una planta con potencial actividad antif\u00fangica.","volume":"32","author":"A Due\u00f1as-Dey\u00e1","year":"Dec. 2020","journal-title":"Revista Cubana de Qu\u00edmica."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"71-81","DOI":"10.1515\/jcim-2014-0011","article-title":"Pharmacognostical standardization and antidiabetic activity of Syzygium cumini (Linn.) barks (Myrtaceae) on streptozotocin-induced diabetic rats.","volume":"11","author":"A Tripathi","year":"2014 Jun","journal-title":"J. Complement. Integr. Med."},{"key":"ref43","first-page":"742-746","article-title":"Toxicological evaluation and oral glucose tolerance test of ethanolic leaf extract of Barleria cristata L. in wistar albino rats.","volume":"2","author":"R Narmadha","year":"2017 Feb. 2","journal-title":"Int. J. Basic Clin. Pharmacol [Internet]."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"e272","DOI":"10.51252\/rsayb.v1i1.272","article-title":"Flavonoides y Fenoles totales con actividad hipoglicemiante en semillas de Syzygium jambos.","volume":"1","author":"M Gonz\u00e1lez-Blas","year":"Jan. 2022","journal-title":"Revista Salud Amaz\u00f3nica y Bienestar."},{"key":"ref30","first-page":"23-30","article-title":"Perfil glic\u00e9mico durante el ayuno en ratas macho-Wistar con diabetes tipo 2.","volume":"83","author":"M Figueroa-Garc\u00eda","year":"Nov. 2016","journal-title":"Revista del Hospital Ju\u00e1rez de M\u00e9xico."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"474-484","DOI":"10.17843\/RPMESP.2023.404.12672","article-title":"Revisi\u00f3n exploratoria sobre la evidencia de cultivos andinos con efecto hipoglucemiante y de sus componentes bioactivos.","volume":"40","author":"D D\u00edaz-N\u00fa\u00f1ez","year":"Dec. 2023","journal-title":"Rev. Peru Med. Exp. Salud Publica."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"087-093","DOI":"10.30574\/WJBPHS.2022.12.2.0194","article-title":"Traditional uses, phytochemistry and pharmacology of Acalypha hispida burm: A systematic review.","volume":"12","author":"P Prajna","year":"Nov. 2022","journal-title":"World J. Biol. Pharm. Health Sci."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"113217","DOI":"10.1016\/J.BIOPHA.2022.113217","article-title":"Exploring the plant-derived bioactive substances as antidiabetic agent: An extensive review.","volume":"152","author":"M Rahman","year":"Aug. 2022","journal-title":"Biomed. Pharmacother."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"252","DOI":"10.3390\/BIOLOGY9090252","article-title":"Bioactive Compounds in Anti-Diabetic Plants: From Herbal Medicine to Modern Drug Discovery.","volume":"9","author":"N Tran","year":"Aug. 2020","journal-title":"Biology (Basel)."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"1-35","DOI":"10.3389\/FENDO.2022.800714\/PDF","article-title":"Antidiabetic Phytochemicals From Medicinal Plants: Prospective Candidates for New Drug Discovery and Development.","volume":"13","author":"S Alam","year":"Feb. 2022","journal-title":"Front. Endocrinol (Lausanne)."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"430","DOI":"10.3390\/BIOM9090430","article-title":"Flavonoids and Their Anti-Diabetic Effects: Cellular Mechanisms and Effects to Improve Blood Sugar Levels.","volume":"9","author":"R Al-Ishaq","year":"Sep. 2019","journal-title":"Biomolecules."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"25-32","DOI":"10.14642\/RENC.2018.24.1.5240","article-title":"Efecto de los compuestos fen\u00f3licos en el metabolismo de los carbohidratos.","volume":"24","author":"M Due\u00f1as Mart\u00edn","year":"2018","journal-title":"Revista Espa\u00f1ola de Nutrici\u00f3n Comunitaria = Spanish Journal of Community Nutrition."},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.26722\/RPMI.2022.V7N4.1","article-title":"Efecto reductor del extracto acuoso de hojas de Justicia spicigera contra la hiperglicemia en ratones BALB\/C.","volume":"7","author":"L N\u00fa\u00f1ez-Tuesta","year":"Dec. 2022","journal-title":"Revista Peruana de Medicina Integrativa."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"40-52","DOI":"10.18684\/rbsaa.v22.n1.2024.2244","article-title":"Regulaci\u00f3n de los niveles de glucosa mediante la inducci\u00f3n de extracto acuoso de Pseudoelephantopus spicatus.","volume":"22","author":"D Acevedo Correa","year":"2024","journal-title":"Biotecnolog\u00eda en el Sector Agropecuario y Agroindustrial: BSAA."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"275-283","DOI":"10.15171\/APB.2016.039","article-title":"Anti-Inflammatory and Antioxidant Activity of Acalypha hispida Leaf and Analysis of its Major Bioactive Polyphenols by HPLC.","volume":"6","author":"M Siraj","year":"Jun. 2016","journal-title":"Adv. Pharm. Bull."},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.16732470","author":"J Morales Ramos","year":"2025","journal-title":"Zenodo."}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/14-800\/v3\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/14-800\/v3\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/14-800\/v3\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:10:09Z","timestamp":1760112609000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/14-800\/v3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,10]]},"references-count":40,"URL":"https:\/\/doi.org\/10.12688\/f1000research.164673.3","relation":{"has-version":[{"id-type":"doi","id":"10.12688\/f1000research.164673.2","asserted-by":"subject"},{"id-type":"doi","id":"10.12688\/f1000research.164673.1","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.188409.r422492","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,10]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/14-800\/v3#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.187885.r414413, Antonio Gal\u00e1n de Mera, Universidad San Pablo-CEU, Madrid, 16 Sep 2025, version 2, indexed","URL":"https:\/\/f1000research.com\/articles\/14-800\/v2#referee-response-414413","order":0,"name":"referee-response-414413","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.181218.r407373, Aku Enam Motto, University of Lom\u00e9, Lom\u00e9, 08 Sep 2025, version 1, 1 approved, 1 not approved","URL":"https:\/\/f1000research.com\/articles\/14-800\/v1#referee-response-407373","order":1,"name":"referee-response-407373","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Jorge Guillermo Morales Ramos<\/b>; \n<i>Posted: 21 Sep 2025<\/i>; Abstract The sex of the rats was added to the summary. Introduction Reformulate It has been reformulated by adding Peruvian plants with hypoglycaemic effects (page 3, paragraph 10), article v2. Materials and methods Authors should follow the structure for greater clarity and understanding. Animals The reviewer's suggestion was accepted, incorporating the sex of the study animals and indicating that they were male. Plant collection and identification The methods of Cerrate and Romagosa et al. were used for the collection of botanical material. &nbsp;Cerrate E. Manera de preparar plantas para un herbario. Lima: Editorial UNMSM; 1969. Romagosa J, Rosales S, Rup&eacute;rez RF, Crespo R. Enciclopedia de medicina naturalista y alternativa; 2000.  Preparation of the aqueous extract Phytochemical screening The methodology mentioned the method followed by Garc&iacute;a Granados (paragraph 7, p.3, ref. 27), which describes the stages of the process. This is a qualitative test, so it is not necessary to write +++ or ---. Absence - and Presence + Version 2 of the article states that the test was qualitative with semi-quantitative estimation (p. 3, paragraph 1, Phytochemical analysis, ref. 28). Experimental design The authors should mention the dose of glucose administered to the rats. Paragraph 2 of the experimental design section indicates that the dose of glucose administered was 5 ml. The authors did not mention whether the manipulation was performed on fasted rats or not. The experimental design section mentions the conditions referred to by the reviewer (p. 7, paragraph 2). It is also necessary to specify the sex of the animals. We appreciate the reviewer's suggestion to include the sex of the rats, which were males paragraph 2(a).  Blood glucose measurement should be extended to 2 hours (30 min, 60 min, 60 min; 120 and 180 min). We appreciate the reviewer's suggestion regarding the inclusion of additional times (120 and 180 minutes). In our study, 30, 60, and 90 minutes were selected because these intervals are widely used in glucose tolerance tests in preclinical models with plant extracts, as they allow both the post-load peak and the early phase of blood glucose return to be captured (Tripathi et al., 2014; Narmadha, et al., 2017). With this design, it was possible to identify the maximum hypoglycaemic effect of the extract in the critical window where changes are most evident. While we recognise that extended measurements up to 120 or 180 minutes may provide additional information, the main objective of this exploratory study was to evaluate the early hypoglycaemic response, which is adequately reflected in the 90 minutes. Undoubtedly, future studies \u2014especially in diabetic models and chronic evaluations\u2014 will include longer times. Tripathi AK, Kohli S. Pharmacognostical standardization and antidiabetic activity of Syzygium cumini (Linn.) barks (Myrtaceae) on streptozotocin-induced diabetic rats. J Complement Integr Med. 2014 Jun;11(2):71-81. doi: 10.1515\/jcim-2014-0011. PMID: 24760764. Narmadha R, Devaki K. Toxicological evaluation and oral glucose tolerance test of ethanolic leaf extract of Barleria cristata L. in wistar albino rats. Int J Basic Clin Pharmacol [Internet]. 2017 Feb. 2 [cited 2025 Sep. 12];2(6):742-6. Available from: https:\/\/www.ijbcp.com\/index.php\/ijbcp\/article\/view\/1359","URL":"https:\/\/f1000research.com\/articles\/14-800\/v3#referee-comment-14637","order":2,"name":"referee-comment-14637","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.187885.r410404, MARIO ANTONIO BOLARTE ARTEAGA, Bolarte Artega Mario Antonio, UNIVERSIDAD CONTINENTAL, Lima, 24 Sep 2025, version 2, indexed","URL":"https:\/\/f1000research.com\/articles\/14-800\/v2#referee-response-410404","order":3,"name":"referee-response-410404","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Jorge Guillermo Morales Ramos<\/b>; \n<i>Posted: 25 Sep 2025<\/i>; We thank the reviewer for approving the article without comments.","URL":"https:\/\/f1000research.com\/articles\/14-800\/v3#referee-comment-14659","order":4,"name":"referee-comment-14659","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This research received institutional funding from the University of San Mart\u00edn de Porres with project code: E21102023008.","order":5,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}