{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:41:48Z","timestamp":1791236508390,"version":"4.1.0"},"update-to":[{"DOI":"10.12688\/f1000research.166336.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000}}],"reference-count":32,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Not applicable"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>\n                    Background Bisphenol A (BPA), a ubiquitous environmental pollutant, is known to induce oxidative stress and disrupt male reproductive function. This study evaluated the ameliorative effects of the ethyl-acetate extract of\n                    <ns3:italic>Bidens pilosa L., Asteraceae<\/ns3:italic>\n                    (EABP) on oxidative stress and reproductive impairment in BPA-exposed male mice. Method GC-MS phytocompounds from EABP were subjected to\n                    <ns3:italic>in-silico<\/ns3:italic>\n                    pharmacokinetic, drug-likeness, and toxicological screening using the SwissAdme and PkCSM web servers. Antioxidant capacity of EABP was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Over a 28-day period, male mice were assigned to six groups. The control group (Group 1) received distilled water, while Group 2 administered BPA at a dose of 100 mg\/kg\/day. Groups 3, 4, and 5 were treated with BPA in combination with EABP at doses of 250, 500, and 1000 mg\/kg\/day, respectively. Group 6 received BPA along with vitamin C at 100 mg\/kg\/day. Various parameters, including body weight, malondialdehyde, catalase, and superoxide dismutase level were assessed. Result Molecular docking analysis revealed that 9,12,15-octadecatrienoic acid, a key phytoconstituent of EABP, exhibited strong binding affinity for both the androgen receptor (AR) and nuclear factor erythroid 2-related factor 2 (NRF2). Its interaction with AR was comparable to that of testosterone, suggesting potential androgenic activity, while its binding to NRF2 mirrored that of vitamin C. The extract\u2019s antioxidant activity was further supported by the DPPH radical scavenging assay, where it demonstrated moderate potency with an IC\u2085\u2080 of 6.11\u03bcg\/mL, compared to 1.25\u03bcg\/mL for vitamin C. in vivo findings showed that treatment with EABP at 250 mg\/kg, 500 mg\/kg, and 1000 mg\/kg restored SOD activity and significantly reduced MDA levels. Catalase (CAT) activity also improved, particularly at the higher dose. Conclusion Collectively, these results indicate that the EABP offers protective effects against BPA-induced oxidative stress, metabolic disturbance, and reproductive dysfunction, possibly through its combined antioxidant and hormone-like properties.\n                  <\/ns3:p>","DOI":"10.12688\/f1000research.166336.2","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T11:10:07Z","timestamp":1764673807000},"page":"771","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Ameliorative Effects of Ethyl-Acetate Extract of Bidens pilosa on Oxidative Stress and Reproductive Impairment in Bisphenol A-Exposed Male Mice: Insight from In-silico, Invitro and In-vivo Studies"],"prefix":"10.12688","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3279-2711","authenticated-orcid":false,"given":"Ismahil Adekunle","family":"Adeniyi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Owu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olufunke","family":"Onaadepo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umar Uthman","family":"Shehu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ishak Abdi","family":"Jama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua Ojodale","family":"Aruwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theophilus","family":"Pius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6624-1286","authenticated-orcid":false,"given":"Ibe Michael","family":"Usman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2025,12,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"240-241","DOI":"10.1016\/j.fertnstert.2023.05.166","article-title":"Options after a failed microsurgical testicular sperm extraction.","volume":"120","author":"M Eisenberg","year":"2023","journal-title":"Fertil. 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Suggestion: why not dock with all the Phyto-compounds from the GC\/MS analysis and from that limit the subsequent protocols to the compounds that show preliminary activity. \n<b>Author\u2019s response<\/b> \u2013We appreciate your comment and acknowledge the limitation of our GC\/MS analysis method, which may have excluded some potentially active compounds. However, given the scope and objectives of our current study, we focused on the most abundant compounds (those above the 2% threshold) to prioritize computational resources and streamline our analysis. While docking all phyto-compounds from the GC\/MS analysis is an excellent approach, it would significantly expand the computational workload, which wasn't feasible within our current project's constraints. Our goal was to identify potential leads from the most prominent compounds, recognizing that further studies can explore a broader range of compounds, including non-volatile ones, in the future.  \n<b>Others<\/b> \n<b>Reviewer Comment <\/b>\n<b>II<\/b> \u2013 Provide the authority for the plant and family at first mention. \n<b>Author\u2019s response<\/b> \u2013 The authority for the plant and family have been captured at first mention \n<b>Reviewer Comment <\/b>\n<b>II<\/b> \u2013 How long was the plant material dried? \n<b>Author\u2019s response<\/b> \u2013 The plant material was dried for 5 days \n<b>Reviewer Comment <\/b>\n<b>II<\/b> \u2013 Suspension of fine powder in 400 mL of water, was for how long? \n<b>Author\u2019s response<\/b> - Suspension of fine powder in 400 mL of water, was for 48 hours \n<b>Reviewer Comment <\/b>\n<b>II<\/b> \u2013 Partitioning of the aqueous extract in different solvent three-times does not guaranty exhaustive extraction \n<b>Author\u2019s response<\/b> \u2013 This is well noted \n<b>Reviewer Comment <\/b>\n<b>II<\/b> \u2013 DPPH assay: The first 6 sentences are not part of the methodology \n<b>Author\u2019s response<\/b> \u2013 The first 6 sentences have been deleted \n<b>Reviewer Comment <\/b>\n<b>II<\/b> \u2013 Statistical analysis: the sentence should be recast to make sense. \n<b>Author\u2019s response<\/b> - The sentence have been rewritten to make sense \n<b>Reviewer Comment <\/b>\n<b>II<\/b> \u2013 Not quite clear why the study was limited to Ethyl acetate fraction following initial partitioning into different solvent system. \n<b>Author\u2019s response<\/b> \u2013 The preference for Ethyl acetate fraction was due to its oil-like composition. \n<b>Reviewer Comment <\/b>\n<b>II<\/b> \u2013 Was the crude EXTRACT used for the GC\/MS? \n<b>Author\u2019s response<\/b> \u2013 The crude extract was not used for GC\/MS due to financial constrain at the time study was conducted","URL":"https:\/\/f1000research.com\/articles\/14-771\/v2#referee-comment-14981","order":1,"name":"referee-comment-14981","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.183308.r410192, Parween Abdulsamad Ismail, University of Salahaddin, Erbil, 24 Sep 2025, version 1, 1 approved, 1 approved with reservations","URL":"https:\/\/f1000research.com\/articles\/14-771\/v1#referee-response-410192","order":2,"name":"referee-response-410192","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Ibe Michael Usman<\/b>; \n<i>Posted: 22 Nov 2025<\/i>; \n<b>Reviewer Comment <\/b>I - This discussion could be expanded to explain the Ameliorative Effects of Ethyl-Acetate Extract of \n<i>Bidens pilosa<\/i> on Oxidative Stress and Reproductive Impairment \n<b>Author\u2019s Response:<\/b> The discussion is now expanded and mechanism of action for \n<i>Bidens pilosa<\/i> involving direct antioxidant activity has now been integrated with reproductive impairment.  \n<b>Reviewer Comment <\/b>I - The conclusions are need to be rewritten to highlight the key contributions of this manuscript \n<b>Author\u2019s Response: <\/b>The conclusion section has been rewritten and the contributions of the study have been stated in the manuscript.","URL":"https:\/\/f1000research.com\/articles\/14-771\/v2#referee-comment-14982","order":3,"name":"referee-comment-14982","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.192058.r449875, Mustofa Ahda, Universitas Ahmad Dahlan, Yogyakarta, Special Region of Yogyakarta, 16 Jan 2026, version 2, indexed","URL":"https:\/\/f1000research.com\/articles\/14-771\/v2#referee-response-449875","order":4,"name":"referee-response-449875","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"The author(s) declared that no grants were involved in supporting this work","order":5,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}