{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T13:08:34Z","timestamp":1780664914638,"version":"3.54.1"},"update-to":[{"DOI":"10.12688\/f1000research.172513.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000}}],"reference-count":52,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"I confirm that I have not received any funding to support this work."}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>\n                    Background Green synthesis of silver nanoparticles (AgNPs) using plant extracts has gained increasing attention as an environmentally friendly alternative to conventional chemical methods.\n                    <ns3:italic>Salix alba L<\/ns3:italic>\n                    contains bioactive phytochemicals that can act as reducing and stabilizing agents during nanoparticle synthesis. This study aimed to synthesize AgNPs using an aqueous bark extract of\n                    <ns3:italic>S. alba<\/ns3:italic>\n                    and to evaluate their antimicrobial and cytogenetic effects. Methods Silver nanoparticles were synthesized by mixing\n                    <ns3:italic>S. alba<\/ns3:italic>\n                    aqueous extract with silver nitrate solution. Nanoparticle formation and surface characteristics were assessed using ultraviolet visible spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Antimicrobial activity against\n                    <ns3:italic>Escherichia coli<\/ns3:italic>\n                    ,\n                    <ns3:italic>Staphylococcus aureus<\/ns3:italic>\n                    , and\n                    <ns3:italic>Candida albicans<\/ns3:italic>\n                    were evaluated using the agar well diffusion method. Cytogenetic effects were assessed in mice bone marrow cells by measuring the mitotic index. Results AFM and SEM analyses showed that AgNP size and morphology varied with silver nitrate concentration, with smaller and more uniform nanoparticles observed at lower concentrations. The synthesized AgNPs exhibited antimicrobial activity against all tested microorganisms, with inhibition zones increasing in a dose dependent manner. Cytogenetic analysis demonstrated a significant reduction in mitotic index in AgNP treated groups at higher concentrations, while\n                    <ns3:italic>S. alba<\/ns3:italic>\n                    extract alone did not induce genotoxic effects and maintained normal or slightly elevated mitotic index values. Conclusions\n                    <ns3:italic>Salix alba L<\/ns3:italic>\n                    mediated silver nanoparticles demonstrated antimicrobial activity and concentration dependent cytogenetic effects. These findings indicate the importance of careful dose optimization when considering biologically synthesized AgNPs for potential applications.\n                  <\/ns3:p>","DOI":"10.12688\/f1000research.172513.2","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T14:04:06Z","timestamp":1780495446000},"page":"331","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Green biosynthesized silver nanoparticles using aqueous extract of Salix alba: antimicrobial and cytogenetic effects on mitosis"],"prefix":"10.12688","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0657-9271","authenticated-orcid":false,"given":"Worood Kamil","family":"Shalash Al Maliky","sequence":"first","affiliation":[{"name":"Biology Department, College of Education for Pure Sciences (Ibn Al-Haitham) Baghdad University of Baghdad"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6618-3557","authenticated-orcid":false,"given":"Jalal","family":"Hasan Mohammed","sequence":"additional","affiliation":[{"name":"College of pharmacy Karbala Karbala Governorate University of Karbala"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamednoruldin","family":"Dh Hazim","sequence":"additional","affiliation":[{"name":"College of Biotechnology, Medical and Molecular Department Baghdad Al-Nahrain University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"982-994","DOI":"10.5530\/ijper.20251041","article-title":"Topical Chitosan-Based Nanogel of Filipendula ulmaria (Meadowsweet) Extract against Bacterial Infections: Development, Characterization, Optimization and in vitro Studies.","volume":"59","author":"G Agrawal","year":"2025","journal-title":"Indian Journal of Pharmaceutical Education and Research."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1-14","DOI":"10.1007\/s12668-025-01977-5","article-title":"Synthesis and Evaluation of PVP-Stabilized Platinum-Doped ZnO Nanoparticles: A Dual Approach for Photocatalytic and Antibacterial Applications.","volume":"15","author":"A Ahmed","year":"2025","journal-title":"BioNanoScience."},{"key":"ref3","first-page":"1-17","article-title":"Eco-friendly fabrication of silver nanoparticles via Acacia mangium: Exploring antioxidant and antibacterial potentials.","author":"A Aji","year":"2025","journal-title":"Chem. 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