{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T03:01:35Z","timestamp":1784948495207,"version":"3.55.0"},"update-to":[{"DOI":"10.12688\/f1000research.173833.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000}}],"reference-count":30,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns5:p>Background The problem of transmissible human herpesvirus infections through blood donation has not been completely resolved by serological testing due to issues such as delayed in seroconversion or false-negative results. This study aimed to develop a cost-effective, In-house designed multiplex real-time PCR assay for the simultaneous detection of Epstein\u2013Barr virus (EBV) and cytomegalovirus (CMV) DNA in pooled blood donor samples. Methods A total of 1000 whole-blood samples were collected from apparently healthy Iraqi blood donors and pooled into mini pools 10(MP10) i.e. 100 MP10 tested by a commercial CMV and EBV multiplex- qPCR detection kit; then retested by In-house designed multiplex qPCR. Fifteen patients who were positive for CMV and fifteen for EBV were considered as positive groups and fifteen healthy individuals as negative groups used to estimate the validity and feasibility of the In-house designed kit. Results Blood donors who tested by the commercial kit were 42% MP10 positive for EBV and 4% positive for CMV while the In-house designed multiplex qPCR results were 40% and 6% for EBV and CMV, respectively. The specificity and sensitivity of the In-house designed kit for the detection of CMV were 97.92% (95% CI 92.6 to 99.7), 100% (95% CI 39.7 to 100), for EBV were 100% (95% CI 93.84 to100.00) and 95.24 % (95% CI 83.84 to 99.42), respectively. Conclusion The rate of EBV was high within Iraqi blood donors while the CMV was moderately low and the In-house designed multiplex qPCR was sensitive and specific for CMV and EBV DNA detection at a much lower cost than that of the commercial, about 10% of the commercial cost. Such a cost-effective and sensitive test is crucial in reducing blood transfusion-associated diseases.<\/ns5:p>","DOI":"10.12688\/f1000research.173833.2","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T11:24:07Z","timestamp":1782386647000},"page":"360","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["In-house designed Multiplex Real-Time PCR Assay Developed for Epstein - Barr virus and Cytomegalovirus DNA detection in Pooled Blood Samples from Iraqi blood donors"],"prefix":"10.12688","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0879-7512","authenticated-orcid":false,"given":"Ghinwa S.","family":"Majid","sequence":"first","affiliation":[{"name":"Biology Baghdad 10001 Alnahrain University, College of sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed S.","family":"Abdulamir","sequence":"additional","affiliation":[{"name":"Baghdad College of Medicine, Al-Nahrain University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iman M.","family":"Aufi","sequence":"additional","affiliation":[{"name":"Baghdad Central Public Health Laboratory"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1-16","DOI":"10.1177\/03000605211034656","article-title":"Cytomegalovirus seroprevalence among blood donors: a systematic review and meta-analysis.","volume":"49","author":"T Adane","year":"2021","journal-title":"J. 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Beast wishes Dr.Ghinwa on behalf on all authors","URL":"https:\/\/f1000research.com\/articles\/15-360\/v2#referee-comment-16746","order":1,"name":"referee-comment-16746","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.201804.r496644, Noor. Hameed Hanoush, University of Anbar, Ramadi, 07 Jul 2026, version 2, indexed","URL":"https:\/\/f1000research.com\/articles\/15-360\/v2#referee-response-496644","order":2,"name":"referee-response-496644","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Ghinwa Majid<\/b>; \n<i>Posted: 24 Jul 2026<\/i>; Dear reviewers, I sincerely appreciate your time and efforts spent for my manuscript evaluation.Thank you for your positive feedback and greatful for your recommendation. Best wishes Dr.Ghinwa On behalf on all authors","URL":"https:\/\/f1000research.com\/articles\/15-360\/v2#referee-comment-16747","order":3,"name":"referee-comment-16747","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"The author(s) declared that no grants were involved in supporting this work","order":4,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}