{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T10:29:25Z","timestamp":1793788165955,"version":"4.6.1"},"reference-count":38,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009392","name":"Prince Sattam bin Abdulaziz University","doi-asserted-by":"publisher","award":["PSAU\/2025\/01\/4950"],"award-info":[{"award-number":["PSAU\/2025\/01\/4950"]}],"id":[{"id":"10.13039\/100009392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>Background Dentinal hypersensitivity is an enigmatic problem that has long bewildered the dental profession and is one domain where man has not yet achieved the elusive \u201cgold standards\u201d of management. Recently, lasers have been used owning to their various modes of action to reduce hypersensitivity. Thus, the aim of the present in vitro study is to evaluate and compare the effect diode laser along with and without Nanocrystalline Hydroxyapatite powder on occlusion of dentinal tubules under scanning electron microscope (SEM). Methodology Twenty specimens were obtained from dentin discs of 2\u00a0mm thickness prepared from the cemento-enamel junction (CEJ) portion of 20 extracted human third molar teeth. The specimens were divided in to four groups of 5 specimens each. The specimens were etched in 37% phosphoric acid for 60\u00a0seconds to simulate hypersensitivity condition. Specimens in Group 1 (control) received no further treatment. Group 2 (DL) specimens received irradiation with 810\u00a0nm diode laser at an output power of 1\u00a0W for 60\u00a0seconds in continuous wave, non-contact mode. Specimens in Group 3 received treatment with a single application of nanocrystalline hydroxyapatite powder alone, whereas, Group 4 received a combination treatment of Diode laser irradiation followed by application of nanocrystalline hydroxyapatite powder. Results The average tubular diameter of dentinal tubules observed in the specimens belonging to Group 1 (control) was 3.14 microns, and in the Group 2 (diode laser) the average dentinal tubular diameter to be 2.28 microns. Mean diameter of dentinal tubules in Group 3 (nanoHA alone) was 2.41 microns. The specimens belonging to Group 4 (DL\u00a0+\u00a0nanoHA) showed average dentinal tubular diameter were 1.74 microns. Conclusion The single application of nanocrystalline hydroxyapatite powder and diode laser irradiation, showed significantly greater tubular occlusion and increased reduction in dentinal tubular diameters showing greater potential in occluding open dentinal tubules when compared to diode laser alone.<\/ns3:p>","DOI":"10.12688\/f1000research.173988.1","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T11:29:18Z","timestamp":1778585358000},"page":"720","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Energy Dispersive Spectroscopy of Open Dentinal Tubules on Application of Nanocrystalline Hydroxyapatite: An In Vitro Study"],"prefix":"10.12688","volume":"15","author":[{"given":"Tripti","family":"Rawat","sequence":"first","affiliation":[{"name":"Periodontics Plot No -107, Pocket - 27, Rohini Sector 24, New Delhi 110085 Clove Dental"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashmi","family":"Srinath","sequence":"additional","affiliation":[{"name":"Department of Periodontics Davangere Karnataka 577004 College of Dental Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N C","family":"Praveen","sequence":"additional","affiliation":[{"name":"Department of Periodontics Davangere Karnataka 577004 College of Dental Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B S","family":"Amisha","sequence":"additional","affiliation":[{"name":"Periodontics 8th main , 11th Cross, Wilson garden Bengaluru Karnataka 560027 Clove Dental"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3920-8082","authenticated-orcid":false,"given":"Inderjit","family":"Murugendrappa Gowdar","sequence":"additional","affiliation":[{"name":"Department of Preventive Dental Sciences,College of Dentistry Al Kharj Riyadh Province 11942 Prince Sattam bin Abdulaziz University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3369-9925","authenticated-orcid":false,"given":"Sultan A.","family":"Almalki","sequence":"additional","affiliation":[{"name":"Department of Preventive Dental Sciences,College of Dentistry Al Kharj Riyadh Province 11942 Prince Sattam bin Abdulaziz University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9623-559X","authenticated-orcid":false,"given":"Khalid","family":"Gufran","sequence":"additional","affiliation":[{"name":"Department of Preventive Dental Sciences,College of Dentistry Al Kharj Riyadh Province 11942 Prince Sattam bin Abdulaziz University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3194-2626","authenticated-orcid":false,"given":"Usha","family":"G V","sequence":"additional","affiliation":[{"name":"Department of Public Health Dentistry Davangere Karnataka 577004 Bapuji Dental College and Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9267-9297","authenticated-orcid":false,"given":"Bhuvaneshwari","family":"Nadar","sequence":"additional","affiliation":[{"name":"Public Health Dentistry Navi Mumbai Maharashtra 400706 Terna Dental College and Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2026,5,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"153-166","DOI":"10.17219\/dmp\/151482","article-title":"Treatment outcome for dentin hypersensitivity with laser therapy: Systematic review and meta-analysis.","volume":"60","author":"L Pion","year":"2023 Jan-Mar","journal-title":"Dent. 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The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}