{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:08:08Z","timestamp":1772168888464,"version":"3.50.1"},"update-to":[{"DOI":"10.12688\/f1000research.17584.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T00:00:00Z","timestamp":1554854400000}}],"reference-count":43,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T00:00:00Z","timestamp":1554854400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009509","name":"Kementerian Riset Teknologi Dan Pendidikan Tinggi Republik Indonesia","doi-asserted-by":"publisher","award":["PenelitianDasarUnggulanPerguruanTinggiNo:24\/UN1\/DITLIT\/DIT-LIT\/LT\/2018"],"award-info":[{"award-number":["PenelitianDasarUnggulanPerguruanTinggiNo:24\/UN1\/DITLIT\/DIT-LIT\/LT\/2018"]}],"id":[{"id":"10.13039\/501100009509","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background:<\/ns4:bold>\n                    Breast cancer is the second leading cause of death in women. Alternative medicine with high efficacy is needed for breast cancer treatments, for example induction of apoptosis using natural products. It has been found that many natural apoptosis-inducing compounds are isolated from marine sponge. The objective of this study is to analyze the ability of extracts of the sponge\n                    <ns4:italic>Ancorina<\/ns4:italic>\n                    sp. to induce apoptosis on human breast cancer T47D cell line and find out its mechanism.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    T47D cells were treated with crude extracts of methanol, dichloromethane:methanol (1:1) and dichloromethane\n                    <ns4:italic>Ancorina<\/ns4:italic>\n                    sp. for 24 h, and doxorubicin was used as a positive control. Methods used for this study were MTT assay to examine cell viability and determine IC\n                    <ns4:sub>50<\/ns4:sub>\n                    of the three extracts, while the percentage of apoptosis and caspase-3 were investigated by flow cytometry.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results:<\/ns4:bold>\n                    IC\n                    <ns4:sub>50<\/ns4:sub>\n                    values of methanol, dichloromethane:methanol (1:1), and dichloromethane extract were 84.25, 121.45, and 99.85\u03bcg\/mL respectively. The percentages of apoptotic cells after treatment with methanol, dichloromethane:methanol (1:1), and dichloromethane extracts were 88.68, 27.54 and 53.63% respectively, whereas the percentage of caspase-3 was 77.87, 12.66 and 12.97%, respectively.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusions:<\/ns4:bold>\n                    These results revealed that all extracts of\n                    <ns4:italic>Ancorina<\/ns4:italic>\n                    sp. have strong or moderate cytotoxicity and have the ability to induce apoptosis on T47D human breast cancer cell line. However, methanol crude extract has high efficacy to induce apoptosis through caspase-3 activation compared to the other extracts. Hence methanol extract warrants further investigation as a natural medicine for human breast cancer.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.17584.2","type":"journal-article","created":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T11:55:23Z","timestamp":1554897323000},"page":"168","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Apoptosis induction on human breast cancer T47D cell line by extracts of Ancorina sp."],"prefix":"10.12688","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2644-8949","authenticated-orcid":false,"given":"Woro Anindito Sri","family":"Tunjung","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Puspa Restu","family":"Sayekti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2019,4,10]]},"reference":[{"key":"ref-1","article-title":"International Agency for Research on Cancer (IARC) Official Website","year":"2018"},{"key":"ref-2","doi-asserted-by":"crossref","first-page":"513-519","DOI":"10.1158\/1535-7163.513.3.4","article-title":"G2 checkpoint abrogators as anticancer drugs.","volume":"3","author":"T Kawabe","year":"2004","journal-title":"Mol Cancer Ther."},{"key":"ref-3","doi-asserted-by":"publisher","first-page":"29-44","DOI":"10.1016\/j.critrevonc.2004.02.002","article-title":"The role of targeted therapy in non-small cell lung cancer.","volume":"51","author":"P Maione","year":"2004","journal-title":"Crit Rev Oncol Hematol."},{"key":"ref-4","doi-asserted-by":"publisher","first-page":"471-94","DOI":"10.1007\/s10456-014-9420-y","article-title":"Anti-angiogenic therapy for cancer: current progress, unresolved questions and future directions.","volume":"17","author":"N Vasudev","year":"2014","journal-title":"Angiogenesis."},{"key":"ref-5","doi-asserted-by":"publisher","first-page":"36-43","DOI":"10.3747\/co.v16i2.428","article-title":"Development of cell-cycle inhibitors for cancer therapy.","volume":"16","author":"M Dickson","year":"2009","journal-title":"Curr Oncol."},{"key":"ref-6","first-page":"173-5","article-title":"[Photodynamic therapy in the treatment of breast cancer].","volume":"33","author":"J P\u0142onka","year":"2012","journal-title":"Pol Merkur Lekarski."},{"key":"ref-7","doi-asserted-by":"publisher","first-page":"263-270","DOI":"10.1093\/carcin\/bgh283","article-title":"Apoptosis in the development and treatment of cancer.","volume":"26","author":"R Gerl","year":"2005","journal-title":"Carcinogenesis."},{"key":"ref-8","doi-asserted-by":"publisher","first-page":"S78-S103","DOI":"10.1016\/j.semcancer.2015.03.001","article-title":"Broad targeting of resistance to apoptosis in cancer.","volume":"35","author":"R Mohammad","year":"2015","journal-title":"Semin Cancer Biol."},{"key":"ref-9","first-page":"212-218","article-title":"Messengers of cell death: apoptotic signaling in health and disease.","volume":"88","author":"D Rossi","year":"2003","journal-title":"Haematologica."},{"key":"ref-10","doi-asserted-by":"publisher","first-page":"1213-1220","DOI":"10.1016\/j.yexcr.2012.02.013","article-title":"The central role of initiator caspase-9 in apoptosis signal transduction and the regulation of its activation and activity on the apoptosome.","volume":"318","author":"M W\u00fcrstle","year":"2012","journal-title":"Exp Cell Res."},{"key":"ref-11","doi-asserted-by":"publisher","first-page":"1580-1606","DOI":"10.3390\/md9091580","article-title":"Recently confirmed apoptosis-inducing lead compounds isolated from marine sponge of potential relevance in cancer treatment.","volume":"9","author":"M Essack","year":"2011","journal-title":"Mar Drugs."},{"key":"ref-12","doi-asserted-by":"publisher","first-page":"456-465","DOI":"10.1007\/s00044-015-1491-9","article-title":"Induction of apoptosis and cell cycle arrest by Negombata magnifica sponge in hepatocellular carcinoma.","volume":"25","author":"H Rady","year":"2016","journal-title":"Med Chem Res."},{"key":"ref-13","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/717959","article-title":"Apoptosis-Inducing Activity of Marine Sponge Haliclona sp. 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Tunjung","year":"2019","journal-title":"OSF."}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/8-168\/v2\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/8-168\/v2\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/8-168\/v2\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T14:11:57Z","timestamp":1663251117000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/8-168\/v2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,10]]},"references-count":43,"URL":"https:\/\/doi.org\/10.12688\/f1000research.17584.2","relation":{"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.20632.r47034","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.20632.r47035","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.20632.r47035","asserted-by":"object"},{"id-type":"doi","id":"10.5256\/f1000research.20632.r47034","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,10]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/8-168\/v2#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.20632.r47034, Sutiman Bambang Sumitro, Department of Biology, Faculty of Sciences, Brawijaya University, Malang, Indonesia, 16 Apr 2019, version 2, indexed","URL":"https:\/\/f1000research.com\/articles\/8-168\/v2#referee-response-47034","order":0,"name":"referee-response-47034","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.20632.r47035, R. Luke Daniels, Department of Biology, The College of Idaho, Caldwell, ID, USA, 12 Apr 2019, version 2, indexed","URL":"https:\/\/f1000research.com\/articles\/8-168\/v2#referee-response-47035","order":1,"name":"referee-response-47035","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was financially supported by Penelitian Dasar UnggulanPerguruan Tinggi No: 24\/UN1\/DITLIT\/DIT-LIT\/LT\/2018 from Indonesia Ministry of Research, Technology and Higher education (to W.A.S.T). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}